(Circular) (Six-base) MAPSORT of: pFN21AB3017.seq Check: 7762 from: 1 to: 7099 pFN21AB3017 7099 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4909 278 Size: 53 83 186 936 3373 2468 Fragments arranged by size: 3373 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3069 4442 1937 Size: 1132 1373 4594 Fragments arranged by size: 4594 1373 1132 Acc65I G'GTAC_C Cuts at: 4420 4420 Size: 7099 AclI AA'CG_TT Cuts at: 1563 5226 5599 1563 Size: 3663 373 3063 Fragments arranged by size: 3663 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2799 2850 3958 5162 6546 6814 1632 Size: 1167 51 1108 1204 1384 268 1917 Fragments arranged by size: 1917 1384 1204 1167 1108 268 51 AfeI AGC'GCT Cuts at: 2669 2669 Size: 7099 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6902 AflIII A'CryG_T Cuts at: 1203 2512 5998 6975 1203 Size: 1309 3486 977 1327 Fragments arranged by size: 3486 1327 1309 977 AgeI A'CCGG_T Cuts at: 5930 5930 Size: 7099 AhdI GACnn_n'nnGTC Cuts at: 5828 5828 Size: 7099 AleI CACnn'nnGTG Cuts at: 1166 3468 4133 1166 Size: 2302 665 4132 Fragments arranged by size: 4132 2302 665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2933 3811 4151 1184 Size: 1749 878 340 4132 Fragments arranged by size: 4132 1749 878 340 AlwNI CAG_nnn'CTG Cuts at: 3079 4842 6414 3079 Size: 1763 1572 3764 Fragments arranged by size: 3764 1763 1572 ApaI G_GGCC'C Cuts at: 3382 3609 3382 Size: 227 6872 ApaLI G'TGCA_C Cuts at: 5156 6312 5156 Size: 1156 5943 ApoI r'AATT_y Cuts at: 1493 4408 4634 4786 6895 1493 Size: 2915 226 152 2109 1697 Fragments arranged by size: 2915 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5653 160 Size: 872 4621 1606 Fragments arranged by size: 4621 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7099 AvaI C'yCGr_G Cuts at: 1870 1943 2268 2525 3827 4424 1870 Size: 73 325 257 1302 597 4545 Fragments arranged by size: 4545 1302 597 325 257 73 AvrII C'CTAG_G Cuts at: 3523 3523 Size: 7099 BamHI G'GATC_C Cuts at: 4429 4796 4429 Size: 367 6732 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2345 2360 2374 2745 3582 Size: 325 217 627 558 15 14 371 837 Cuts at: 3582 4420 5875 618 Size: 838 1455 1842 Fragments arranged by size: 1842 1455 838 837 627 558 371 325 217 15 14 BanII G_rGCy'C Cuts at: 1391 2301 2415 3360 3382 3592 3609 3668 3840 Size: 910 114 945 22 210 17 59 172 Cuts at: 3840 4418 1391 Size: 578 4072 Fragments arranged by size: 4072 945 910 578 210 172 114 59 22 17 BbeI G_GCGC'C Cuts at: 2349 2378 2749 2349 Size: 29 371 6699 Fragments arranged by size: 6699 371 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3165 928 Size: 971 1266 4862 Fragments arranged by size: 4862 1266 971 BbvCI CC'TCA_GC Cuts at: 2445 3270 2445 Size: 825 6274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3267 3301 5290 5324 1903 Size: 34 1330 34 1989 34 3678 Fragments arranged by size: 3678 1989 1330 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2444 4991 6201 2444 Size: 2547 1210 3342 Fragments arranged by size: 3342 2547 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7099 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5710 136 Size: 107 122 71 631 605 4038 1525 Fragments arranged by size: 4038 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7094 7094 Size: 7099 BlpI GC'TnA_GC Cuts at: 2336 4516 2336 Size: 2180 4919 Bme1580I G_kGCm'C Cuts at: 1790 2532 2934 3382 3514 3609 4018 5160 6316 Size: 742 402 448 132 95 409 1142 1156 Cuts at: 6316 1790 Size: 2573 Fragments arranged by size: 2573 1156 1142 742 448 409 402 132 95 BmgBI CAC'GTC Cuts at: 1318 3295 1318 Size: 1977 5122 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2916 3229 4830 Size: 416 53 83 399 24 1499 313 1601 Cuts at: 4830 5788 442 Size: 958 1753 Fragments arranged by size: 1753 1601 1499 958 416 399 313 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7099 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3577 3577 Size: 7099 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 2312 2408 2684 2854 3112 5759 1239 Size: 1064 9 96 276 170 258 2647 2579 Fragments arranged by size: 2647 2579 1064 276 258 170 96 9 Bpu10I CC'TnA_GC Cuts at: 2045 2445 2483 3270 2045 Size: 400 38 787 5874 Fragments arranged by size: 5874 787 400 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5224 6089 6387 5224 Size: 865 298 5936 Fragments arranged by size: 5936 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4141 5762 882 Size: 617 2642 1621 2219 Fragments arranged by size: 2642 2219 1621 617 BsaAI yAC'GTr Cuts at: 493 1204 4890 493 Size: 711 3686 2702 Fragments arranged by size: 3686 2702 711 BsaBI GATnn'nnATC Cuts at: 7093 7093 Size: 7099 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2346 2375 2746 Size: 53 83 186 936 537 276 29 371 Cuts at: 2746 4119 4906 5288 5923 275 Size: 1373 787 382 635 1451 Fragments arranged by size: 1451 1373 936 787 635 537 382 371 276 186 83 53 29 BsaWI w'CCGG_w Cuts at: 5532 5930 6204 6351 6762 5532 Size: 398 274 147 411 5869 Fragments arranged by size: 5869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6874 Fragments arranged by size: 6874 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2300 2309 3410 1186 Size: 1084 30 9 1101 4875 Fragments arranged by size: 4875 1101 1084 30 9 BseYI C'CCAG_C Cuts at: 2128 2180 2367 2415 2888 3447 4189 4222 6302 Size: 52 187 48 473 559 742 33 2080 Cuts at: 6302 6798 2128 Size: 496 2429 Fragments arranged by size: 2429 2080 742 559 496 473 187 52 48 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2793 3634 3978 1163 Size: 573 1057 841 344 4284 Fragments arranged by size: 4284 1057 841 573 344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4134 4549 5310 6338 1540 Size: 275 186 2133 415 761 1028 2301 Fragments arranged by size: 2301 2133 1028 761 415 275 186 BsiHKAI G_wGCw'C Cuts at: 2624 3545 3668 3904 4140 5160 5245 6316 2624 Size: 921 123 236 236 1020 85 1071 3407 Fragments arranged by size: 3407 1071 1020 921 236 236 123 85 BsmI GAATG_Cn' Cuts at: 3800 4610 4703 3800 Size: 810 93 6196 Fragments arranged by size: 6196 810 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2415 2532 2624 2934 3360 3382 Size: 399 511 114 117 92 310 426 22 Cuts at: 3382 3514 3545 3592 3609 3668 3840 3904 4018 Size: 132 31 47 17 59 172 64 114 Cuts at: 4018 4140 4418 5160 5245 6316 1391 Size: 122 278 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 511 426 399 310 278 172 132 122 117 114 114 92 85 64 59 47 31 22 17 BspEI T'CCGG_A Cuts at: 6762 6762 Size: 7099 BspHI T'CATG_A Cuts at: 4988 6718 4988 Size: 1730 5369 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4454 844 Size: 3610 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2335 2734 4075 4986 1394 Size: 429 512 399 1341 911 3507 Fragments arranged by size: 3507 1341 911 512 429 399 BsrDI GCAATG_nn' Cuts at: 65 1248 2956 5594 5768 65 Size: 1183 1708 2638 174 1396 Fragments arranged by size: 2638 1708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4242 5743 5930 1700 Size: 110 2432 1501 187 2869 Fragments arranged by size: 2869 2432 1501 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7099 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7099 BssSI C'ACGA_G Cuts at: 5159 6171 5159 Size: 1012 6087 BstAPI GCAn_nnn'nTGC Cuts at: 794 2317 2536 3723 3789 3873 794 Size: 1523 219 1187 66 84 4020 Fragments arranged by size: 4020 1523 1187 219 84 66 BstBI TT'CG_AA Cuts at: 5903 5903 Size: 7099 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7099 BstXI CCAn_nnnn'nTGG Cuts at: 2371 2662 2888 3136 4189 2371 Size: 291 226 248 1053 5281 Fragments arranged by size: 5281 1053 291 248 226 BstYI r'GATC_y Cuts at: 1972 2076 4429 4796 5181 5198 6650 6736 6748 Size: 104 2353 367 385 17 1452 86 12 Cuts at: 6748 7094 1972 Size: 346 1977 Fragments arranged by size: 2353 1977 1452 385 367 346 104 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3368 3368 Size: 7099 BtgI C'CryG_G Cuts at: 513 1065 2004 2302 2350 513 Size: 552 939 298 48 5262 Fragments arranged by size: 5262 939 552 298 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6360 Fragments arranged by size: 6360 683 56 BtsI GCAGTG_nn' Cuts at: 2932 3059 3343 3445 3463 3475 3622 3661 3805 Size: 127 284 102 18 12 147 39 144 Cuts at: 3805 3850 4221 4615 5409 5429 2932 Size: 45 371 394 794 20 4602 Fragments arranged by size: 4602 794 394 371 284 147 144 127 102 45 39 20 18 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7099 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7064 DraI TTT'AAA Cuts at: 4403 4403 Size: 7099 DraIII CAC_nnn'GTG Cuts at: 2698 3423 2698 Size: 725 6374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6106 809 Size: 507 4790 1802 Fragments arranged by size: 4790 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2305 3475 3517 Size: 54 1425 50 275 18 475 1170 42 Cuts at: 3517 3990 4546 5435 7064 8 Size: 473 556 889 1629 43 Fragments arranged by size: 1629 1425 1170 889 556 475 473 275 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 4546 1537 Size: 275 2734 4090 Fragments arranged by size: 4090 2734 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3202 3248 5029 1353 Size: 1849 46 1781 3423 Fragments arranged by size: 3423 1849 1781 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2122 2243 5686 6060 6206 387 Size: 1064 138 533 121 3443 374 146 1280 Fragments arranged by size: 3443 1280 1064 533 374 146 138 121 EcoICRI GAG'CTC Cuts at: 3666 3666 Size: 7099 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2312 2408 2684 2799 2850 2854 Size: 393 671 9 96 276 115 51 4 Cuts at: 2854 3112 3958 5162 5759 6546 6814 1239 Size: 258 846 1204 597 787 268 1524 Fragments arranged by size: 1524 1204 846 787 671 597 393 276 268 258 115 96 51 9 4 EcoO109I rG'GnC_Cy Cuts at: 2133 3010 3245 3379 3605 3606 3969 2133 Size: 877 235 134 226 1 363 5263 Fragments arranged by size: 5263 877 363 235 226 134 1 EcoRI G'AATT_C Cuts at: 4408 4786 4408 Size: 378 6721 FspI TGC'GCA Cuts at: 2573 5605 2573 Size: 3032 4067 FspAI rTGC'GCAy Cuts at: 2573 2573 Size: 7099 HaeII r_GCGC'y Cuts at: 1678 2242 2349 2378 2671 2749 6246 1678 Size: 564 107 29 293 78 3497 2531 Fragments arranged by size: 3497 2531 564 293 107 78 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3560 3592 4116 4148 5740 Size: 32 135 32 1988 32 524 32 1592 Cuts at: 5740 5772 5814 5846 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1988 1592 524 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4443 4689 669 Size: 988 281 2505 246 3079 Fragments arranged by size: 3079 2505 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7099 HpaI GTT'AAC Cuts at: 4689 4689 Size: 7099 KasI G'GCGC_C Cuts at: 2345 2374 2745 2345 Size: 29 371 6699 Fragments arranged by size: 6699 371 29 KpnI G_GTAC'C Cuts at: 4424 4424 Size: 7099 MfeI C'AATT_G Cuts at: 4698 4698 Size: 7099 MluI A'CGCG_T Cuts at: 2512 6975 2512 Size: 4463 2636 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6213 6397 1305 Size: 4908 184 2007 Fragments arranged by size: 4908 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3477 3992 10 Size: 54 1425 343 1645 515 3117 Fragments arranged by size: 3117 1645 1425 515 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2915 3468 3765 3793 4023 4133 5057 Size: 648 1749 553 297 28 230 110 924 Cuts at: 5057 5416 5575 518 Size: 359 159 2042 Fragments arranged by size: 2042 1749 924 648 553 359 297 230 159 110 28 MspA1I CmG'CkG Cuts at: 1606 2184 2281 2304 2352 2371 2383 2888 3507 Size: 578 97 23 48 19 12 505 619 Cuts at: 3507 3651 3699 3873 4093 4515 5192 6340 6585 Size: 144 48 174 220 422 677 1148 245 Cuts at: 6585 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 619 578 505 422 245 220 174 144 97 48 48 23 19 12 NarI GG'CG_CC Cuts at: 2346 2375 2746 2346 Size: 29 371 6699 Fragments arranged by size: 6699 371 29 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5608 Fragments arranged by size: 5608 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7099 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7099 NotI GC'GGCC_GC Cuts at: 4546 4546 Size: 7099 NspI r_CATG'y Cuts at: 1132 1229 3792 4457 6002 1132 Size: 97 2563 665 1545 2229 Fragments arranged by size: 2563 2229 1545 665 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7099 PciI A'CATG_T Cuts at: 5998 5998 Size: 7099 PflMI CCAn_nnn'nTGG Cuts at: 1108 2103 3883 1108 Size: 995 1780 4324 Fragments arranged by size: 4324 1780 995 PmeI GTTT'AAAC Cuts at: 4403 4403 Size: 7099 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5168 5200 6681 6713 5168 Size: 32 1481 32 5554 Fragments arranged by size: 5554 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2133 3245 2133 Size: 1112 5987 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7099 PsiI TTA'TAA Cuts at: 3162 4669 3162 Size: 1507 5592 PspOMI G'GGCC_C Cuts at: 3378 3605 3378 Size: 227 6872 PspXI vC'TCGA_Gb Cuts at: 2268 2268 Size: 7099 PstI C_TGCA'G Cuts at: 830 2325 2553 2805 3022 3106 3470 3650 3656 Size: 1495 228 252 217 84 364 180 6 Cuts at: 3656 3845 4004 4451 830 Size: 189 159 447 3478 Fragments arranged by size: 3478 1495 447 364 252 228 217 189 180 159 84 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7099 PvuII CAG'CTG Cuts at: 2184 2371 2888 3507 3651 3699 4093 2184 Size: 187 517 619 144 48 394 5190 Fragments arranged by size: 5190 619 517 394 187 144 48 SacI G_AGCT'C Cuts at: 3668 3668 Size: 7099 SacII CC_GC'GG Cuts at: 2305 2353 2305 Size: 48 7051 SalI G'TCGA_C Cuts at: 1936 4441 1936 Size: 2505 4594 SbfI CC_TGCA'GG Cuts at: 4451 4451 Size: 7099 ScaI AGT'ACT Cuts at: 5347 7059 5347 Size: 1712 5387 SfcI C'TryA_G Cuts at: 826 1045 2321 2549 2801 2959 3018 3102 3466 Size: 219 1276 228 252 158 59 84 364 Cuts at: 3466 3646 3652 3841 4000 4447 5582 6263 6454 Size: 180 6 189 159 447 1135 681 191 Cuts at: 6454 826 Size: 1471 Fragments arranged by size: 1471 1276 1135 681 447 364 252 228 219 191 189 180 159 158 84 59 6 SfoI GGC'GCC Cuts at: 2347 2376 2747 2347 Size: 29 371 6699 Fragments arranged by size: 6699 371 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7099 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7099 SmaI CCC'GGG Cuts at: 1872 4426 1872 Size: 2554 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2268 5203 6104 6366 820 Size: 197 926 325 2935 901 262 1553 Fragments arranged by size: 2935 1553 926 901 325 262 197 SnaBI TAC'GTA Cuts at: 493 4890 493 Size: 4397 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7099 SphI G_CATG'C Cuts at: 1132 3792 4457 1132 Size: 2660 665 3774 Fragments arranged by size: 3774 2660 665 SspI AAT'ATT Cuts at: 5 52 5023 5 Size: 47 4971 2081 Fragments arranged by size: 4971 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7099 StyI C'CwwG_G Cuts at: 513 1065 2004 3382 3523 4038 4538 513 Size: 552 939 1378 141 515 500 3074 Fragments arranged by size: 3074 1378 939 552 515 500 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2381 2576 3356 3461 3570 5126 5143 Size: 1255 801 195 780 105 109 1556 17 Cuts at: 5143 5296 5481 5926 325 Size: 153 185 445 1498 Fragments arranged by size: 1556 1498 1255 801 780 445 195 185 153 109 105 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2466 4004 5345 Size: 274 80 33 51 1443 489 1538 1341 Cuts at: 5345 7057 96 Size: 1712 138 Fragments arranged by size: 1712 1538 1443 1341 489 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2442 2474 3566 3598 4348 4380 7059 7091 2442 Size: 32 1092 32 750 32 2679 32 2450 Fragments arranged by size: 2679 2450 1092 750 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7099 XbaI T'CTAG_A Cuts at: 4435 4435 Size: 7099 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2825 3470 4030 2825 Size: 645 560 5894 Fragments arranged by size: 5894 645 560 XhoI C'TCGA_G Cuts at: 1943 2268 1943 Size: 325 6774 XmaI C'CCGG_G Cuts at: 1870 4424 1870 Size: 2554 4545 XmnI GAAnn'nnTTC Cuts at: 5228 5228 Size: 7099 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4907 276 Size: 53 83 186 936 3373 2468 Fragments arranged by size: 3373 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI