(Circular) (Six-base) MAPSORT of: pFN21AB3003.seq Check: 2861 from: 1 to: 6604 pFN21AB3003 6604 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4414 278 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3947 1937 Size: 2010 4594 Acc65I G'GTAC_C Cuts at: 3204 3925 3204 Size: 721 5883 AclI AA'CG_TT Cuts at: 1563 4731 5104 1563 Size: 3168 373 3063 Fragments arranged by size: 3168 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2563 2948 3387 4667 6051 6319 1632 Size: 931 385 439 1280 1384 268 1917 Fragments arranged by size: 1917 1384 1280 931 439 385 268 AfeI AGC'GCT Cuts at: 2375 2375 Size: 6604 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6407 AflIII A'CryG_T Cuts at: 1203 5503 6480 1203 Size: 4300 977 1327 Fragments arranged by size: 4300 1327 977 AgeI A'CCGG_T Cuts at: 5435 5435 Size: 6604 AhdI GACnn_n'nnGTC Cuts at: 5333 5333 Size: 6604 AleI CACnn'nnGTG Cuts at: 1166 3258 1166 Size: 2092 4512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6604 AlwNI CAG_nnn'CTG Cuts at: 2867 3614 4347 5919 2867 Size: 747 733 1572 3552 Fragments arranged by size: 3552 1572 747 733 ApaLI G'TGCA_C Cuts at: 4661 5817 4661 Size: 1156 5448 ApoI r'AATT_y Cuts at: 1493 3913 4139 4291 6400 1493 Size: 2420 226 152 2109 1697 Fragments arranged by size: 2420 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5158 160 Size: 872 4126 1606 Fragments arranged by size: 4126 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 AvaI C'yCGr_G Cuts at: 1870 1943 2871 2901 2931 3066 3722 3929 1870 Size: 73 928 30 30 135 656 207 4545 Fragments arranged by size: 4545 928 656 207 135 73 30 30 AvrII C'CTAG_G Cuts at: 3124 3124 Size: 6604 BamHI G'GATC_C Cuts at: 3775 3934 4301 3775 Size: 159 367 6078 Fragments arranged by size: 6078 367 159 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2912 3204 3892 3925 5380 Size: 325 217 627 1125 292 688 33 1455 Cuts at: 5380 618 Size: 1842 Fragments arranged by size: 1842 1455 1125 688 627 325 292 217 33 BanII G_rGCy'C Cuts at: 1391 2572 2922 3621 3723 3923 1391 Size: 1181 350 699 102 200 4072 Fragments arranged by size: 4072 1181 699 350 200 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2132 2777 3546 928 Size: 971 233 645 769 3986 Fragments arranged by size: 3986 971 769 645 233 BbvCI CC'TCA_GC Cuts at: 2674 3479 2674 Size: 805 5799 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4795 4829 1903 Size: 34 50 34 2774 34 3678 Fragments arranged by size: 3678 2774 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4496 5706 4496 Size: 1210 5394 BclI T'GATC_A Cuts at: 1548 3756 1548 Size: 2208 4396 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2307 3135 5215 Size: 107 122 71 631 605 635 828 2080 Cuts at: 5215 136 Size: 1525 Fragments arranged by size: 2080 1525 828 635 631 605 122 107 71 BglII A'GATC_T Cuts at: 6599 6599 Size: 6604 BlpI GC'TnA_GC Cuts at: 2197 4021 2197 Size: 1824 4780 Bme1580I G_kGCm'C Cuts at: 1790 4665 5821 1790 Size: 2875 1156 2573 Fragments arranged by size: 2875 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2597 2855 4335 Size: 416 53 83 399 24 1180 258 1480 Cuts at: 4335 5293 442 Size: 958 1753 Fragments arranged by size: 1753 1480 1180 958 416 399 258 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6604 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2253 2636 2667 2674 3380 5264 1239 Size: 1014 383 31 7 706 1884 2579 Fragments arranged by size: 2579 1884 1014 706 383 31 7 Bpu10I CC'TnA_GC Cuts at: 2674 3479 3615 2674 Size: 805 136 5663 Fragments arranged by size: 5663 805 136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2063 2455 4729 5594 5892 2063 Size: 392 2274 865 298 2775 Fragments arranged by size: 2775 2274 865 392 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2812 3365 5267 882 Size: 617 1313 553 1902 2219 Fragments arranged by size: 2219 1902 1313 617 553 BsaAI yAC'GTr Cuts at: 493 1204 4395 493 Size: 711 3191 2702 Fragments arranged by size: 3191 2702 711 BsaBI GATnn'nnATC Cuts at: 6598 6598 Size: 6604 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4411 4793 5428 275 Size: 53 83 186 936 2878 382 635 1451 Fragments arranged by size: 2878 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5037 5435 5709 5856 6267 5037 Size: 398 274 147 411 5374 Fragments arranged by size: 5374 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3378 3408 3794 3824 1178 Size: 30 165 30 1975 30 386 30 3958 Fragments arranged by size: 3958 1975 386 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2549 2662 2919 2960 3251 3496 3542 Size: 988 375 113 257 41 291 245 46 Cuts at: 3542 3803 1186 Size: 261 3987 Fragments arranged by size: 3987 988 375 291 261 257 245 113 46 41 BseYI C'CCAG_C Cuts at: 2240 2376 2620 2777 2793 2848 2950 3338 3779 Size: 136 244 157 16 55 102 388 441 Cuts at: 3779 5807 6303 2240 Size: 2028 496 2541 Fragments arranged by size: 2541 2028 496 441 388 244 157 136 102 55 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2085 1163 Size: 573 349 5682 Fragments arranged by size: 5682 573 349 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4054 4815 5843 1540 Size: 275 186 2053 761 1028 2301 Fragments arranged by size: 2301 2053 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2878 3396 3621 3723 4665 4750 5821 2878 Size: 518 225 102 942 85 1071 3661 Fragments arranged by size: 3661 1071 942 518 225 102 85 BsmI GAATG_Cn' Cuts at: 3818 4115 4208 3818 Size: 297 93 6214 Fragments arranged by size: 6214 297 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2572 2878 2922 3396 3621 3723 3923 Size: 399 782 306 44 474 225 102 200 Cuts at: 3923 4665 4750 5821 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 782 742 474 399 306 225 200 102 85 44 BspEI T'CCGG_A Cuts at: 6267 6267 Size: 6604 BspHI T'CATG_A Cuts at: 4493 6223 4493 Size: 1730 4874 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3959 844 Size: 3115 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3009 3679 4491 1394 Size: 429 1186 670 812 3507 Fragments arranged by size: 3507 1186 812 670 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3856 5099 5273 65 Size: 1183 2608 1243 174 1396 Fragments arranged by size: 2608 1396 1243 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5248 5435 1700 Size: 110 3438 187 2869 Fragments arranged by size: 3438 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6604 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6604 BssSI C'ACGA_G Cuts at: 4664 5676 4664 Size: 1012 5592 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6604 BstBI TT'CG_AA Cuts at: 5408 5408 Size: 6604 BstEII G'GTnAC_C Cuts at: 1184 2269 1184 Size: 1085 5519 BstXI CCAn_nnnn'nTGG Cuts at: 2137 3884 2137 Size: 1747 4857 BstYI r'GATC_y Cuts at: 1972 2013 2958 3775 3837 3934 4301 4686 4703 Size: 41 945 817 62 97 367 385 17 Cuts at: 4703 6155 6241 6253 6599 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 945 817 385 367 346 97 86 62 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 3558 513 Size: 552 2493 3559 Fragments arranged by size: 3559 2493 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5865 Fragments arranged by size: 5865 683 56 BtsI GCAGTG_nn' Cuts at: 2311 2892 3238 4120 4914 4934 2311 Size: 581 346 882 794 20 3981 Fragments arranged by size: 3981 882 794 581 346 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6604 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6569 DraI TTT'AAA Cuts at: 3908 3908 Size: 6604 DraIII CAC_nnn'GTG Cuts at: 2176 2176 Size: 6604 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2258 2351 5611 809 Size: 507 942 93 3260 1802 Fragments arranged by size: 3260 1802 942 507 93 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2515 3053 3520 Size: 54 1425 50 275 18 685 538 467 Cuts at: 3520 4051 4940 6569 8 Size: 531 889 1629 43 Fragments arranged by size: 1629 1425 889 685 538 531 467 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4051 1537 Size: 275 2239 4090 Fragments arranged by size: 4090 2239 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2579 2732 3363 4534 1353 Size: 1226 153 631 1171 3423 Fragments arranged by size: 3423 1226 1171 631 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2006 2012 3464 3697 5191 5565 Size: 1064 138 417 6 1452 233 1494 374 Cuts at: 5565 5711 387 Size: 146 1280 Fragments arranged by size: 1494 1452 1280 1064 417 374 233 146 138 6 EcoICRI GAG'CTC Cuts at: 3619 3721 3619 Size: 102 6502 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2253 2563 2636 2667 2674 2948 3380 Size: 393 621 310 73 31 7 274 432 Cuts at: 3380 3387 4667 5264 6051 6319 1239 Size: 7 1280 597 787 268 1524 Fragments arranged by size: 1524 1280 787 621 597 432 393 310 274 268 73 31 7 7 EcoNI CCTnn'n_nnAGG Cuts at: 3199 3199 Size: 6604 EcoO109I rG'GnC_Cy Cuts at: 2260 2482 2627 3028 3121 3793 2260 Size: 222 145 401 93 672 5071 Fragments arranged by size: 5071 672 401 222 145 93 EcoRI G'AATT_C Cuts at: 3913 4291 3913 Size: 378 6226 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3664 3664 Size: 6604 FspI TGC'GCA Cuts at: 5110 5110 Size: 6604 HaeII r_GCGC'y Cuts at: 1678 2241 2377 2550 5751 1678 Size: 563 136 173 3201 2531 Fragments arranged by size: 3201 2531 563 173 136 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2471 2503 3126 3158 3233 Size: 32 135 32 899 32 623 32 75 Cuts at: 3233 3265 3794 3826 5245 5277 5319 5351 1373 Size: 32 529 32 1419 32 42 32 2626 Fragments arranged by size: 2626 1419 899 623 529 135 75 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3948 4194 669 Size: 988 281 2010 246 3079 Fragments arranged by size: 3079 2010 988 281 246 HindIII A'AGCT_T Cuts at: 748 2510 3284 748 Size: 1762 774 4068 Fragments arranged by size: 4068 1762 774 HpaI GTT'AAC Cuts at: 4194 4194 Size: 6604 KpnI G_GTAC'C Cuts at: 3208 3929 3208 Size: 721 5883 MfeI C'AATT_G Cuts at: 4203 4203 Size: 6604 MluI A'CGCG_T Cuts at: 6480 6480 Size: 6604 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 2727 2861 5718 5902 1305 Size: 825 597 134 2857 184 2007 Fragments arranged by size: 2857 2007 825 597 184 134 MscI TGG'CCA Cuts at: 10 64 1489 1832 2517 3522 10 Size: 54 1425 343 685 1005 3092 Fragments arranged by size: 3092 1425 1005 685 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 3258 3769 4562 4921 5080 518 Size: 648 969 1123 511 793 359 159 2042 Fragments arranged by size: 2042 1123 969 793 648 511 359 159 MspA1I CmG'CkG Cuts at: 1606 2231 2642 2797 3258 3267 3332 4020 4697 Size: 625 411 155 461 9 65 688 677 Cuts at: 4697 5845 6090 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 688 677 625 461 411 245 155 65 9 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6052 NdeI CA'TA_TG Cuts at: 387 387 Size: 6604 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6604 NotI GC'GGCC_GC Cuts at: 4051 4051 Size: 6604 NspI r_CATG'y Cuts at: 1132 1229 2335 3181 3962 5507 1132 Size: 97 1106 846 781 1545 2229 Fragments arranged by size: 2229 1545 1106 846 781 97 PasI CC'CwG_GG Cuts at: 1348 3668 1348 Size: 2320 4284 PciI A'CATG_T Cuts at: 5503 5503 Size: 6604 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6604 PfoI T'CCnGG_A Cuts at: 3396 3396 Size: 6604 PmeI GTTT'AAAC Cuts at: 3908 3908 Size: 6604 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4673 4705 6186 6218 4673 Size: 32 1481 32 5059 Fragments arranged by size: 5059 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2260 2627 3121 2260 Size: 367 494 5743 Fragments arranged by size: 5743 494 367 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6604 PsiI TTA'TAA Cuts at: 4174 4174 Size: 6604 PspXI vC'TCGA_Gb Cuts at: 3722 3722 Size: 6604 PstI C_TGCA'G Cuts at: 830 2042 3956 830 Size: 1212 1914 3478 Fragments arranged by size: 3478 1914 1212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6604 PvuII CAG'CTG Cuts at: 2231 2642 2797 3332 2231 Size: 411 155 535 5503 Fragments arranged by size: 5503 535 411 155 SacI G_AGCT'C Cuts at: 3621 3723 3621 Size: 102 6502 SalI G'TCGA_C Cuts at: 1936 3946 1936 Size: 2010 4594 SbfI CC_TGCA'GG Cuts at: 3956 3956 Size: 6604 ScaI AGT'ACT Cuts at: 4852 6564 4852 Size: 1712 4892 SfcI C'TryA_G Cuts at: 826 1045 2038 2144 2410 3841 3952 5087 5768 Size: 219 993 106 266 1431 111 1135 681 Cuts at: 5768 5959 826 Size: 191 1471 Fragments arranged by size: 1471 1431 1135 993 681 266 219 191 111 106 SfiI GGCCn_nnn'nGGCC Cuts at: 3135 3135 Size: 6604 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6604 SmaI CCC'GGG Cuts at: 1872 3068 3931 1872 Size: 1196 863 4545 Fragments arranged by size: 4545 1196 863 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 2434 3722 4708 5609 5871 Size: 197 926 135 356 1288 986 901 262 Cuts at: 5871 820 Size: 1553 Fragments arranged by size: 1553 1288 986 926 901 356 262 197 135 SnaBI TAC'GTA Cuts at: 493 4395 493 Size: 3902 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6604 SphI G_CATG'C Cuts at: 1132 3181 3962 1132 Size: 2049 781 3774 Fragments arranged by size: 3774 2049 781 SspI AAT'ATT Cuts at: 5 52 4528 5 Size: 47 4476 2081 Fragments arranged by size: 4476 2081 47 StuI AGG'CCT Cuts at: 1517 3129 3248 1517 Size: 1612 119 4873 Fragments arranged by size: 4873 1612 119 StyI C'CwwG_G Cuts at: 513 1065 2112 3124 4043 513 Size: 552 1047 1012 919 3074 Fragments arranged by size: 3074 1047 1012 919 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3148 4631 4648 4801 4986 5431 325 Size: 1255 1568 1483 17 153 185 445 1498 Fragments arranged by size: 1568 1498 1483 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3111 4850 6562 Size: 274 80 33 51 1443 1134 1739 1712 Cuts at: 6562 96 Size: 138 Fragments arranged by size: 1739 1712 1443 1134 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3378 3410 6564 6596 3378 Size: 32 3154 32 3386 Fragments arranged by size: 3386 3154 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2384 1316 Size: 1068 5536 XbaI T'CTAG_A Cuts at: 3940 3940 Size: 6604 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3530 3530 Size: 6604 XhoI C'TCGA_G Cuts at: 1943 3722 1943 Size: 1779 4825 XmaI C'CCGG_G Cuts at: 1870 3066 3929 1870 Size: 1196 863 4545 Fragments arranged by size: 4545 1196 863 XmnI GAAnn'nnTTC Cuts at: 2073 3002 4733 2073 Size: 929 1731 3944 Fragments arranged by size: 3944 1731 929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4412 276 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI