Restriction map for
pFN21AB3003
(Linear) (Six-base) MAPSORT of: KIBB3003.seq Check: 827 from: 1 to: 1899
KIBB3003.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
Acc65I G'GTAC_C
Cuts at: 0 1199 1899
Size: 1199 700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 943 1382 1899
Size: 558 385 439 517
Fragments arranged by size:
558 517 439 385
AfeI AGC'GCT
Cuts at: 0 370 1899
Size: 370 1529
AleI CACnn'nnGTG
Cuts at: 0 1253 1899
Size: 1253 646
AlwNI CAG_nnn'CTG
Cuts at: 0 862 1609 1899
Size: 862 747 290
Fragments arranged by size:
862 747 290
AvaI C'yCGr_G
Cuts at: 0 866 896 926 1061 1717 1899
Size: 866 30 30 135 656 182
Fragments arranged by size:
866 656 182 135 30 30
AvrII C'CTAG_G
Cuts at: 0 1119 1899
Size: 1119 780
BamHI G'GATC_C
Cuts at: 0 1770 1899
Size: 1770 129
BanI G'GyrC_C
Cuts at: 0 907 1199 1887 1899
Size: 907 292 688 12
Fragments arranged by size:
907 688 292 12
BanII G_rGCy'C
Cuts at: 0 567 917 1616 1718 1899
Size: 567 350 699 102 181
Fragments arranged by size:
699 567 350 181 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 127 772 1541 1899
Size: 127 645 769 358
Fragments arranged by size:
769 645 358 127
BbvCI CC'TCA_GC
Cuts at: 0 669 1474 1899
Size: 669 805 425
Fragments arranged by size:
805 669 425
BclI T'GATC_A
Cuts at: 0 1751 1899
Size: 1751 148
BglI GCCn_nnn'nGGC
Cuts at: 0 302 1130 1899
Size: 302 828 769
Fragments arranged by size:
828 769 302
BlpI GC'TnA_GC
Cuts at: 0 192 1899
Size: 192 1707
BmrI ACTGGGnnnn_n'
Cuts at: 0 592 850 1899
Size: 592 258 1049
Fragments arranged by size:
1049 592 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 631 662 669 1375 1899
Size: 248 383 31 7 706 524
Fragments arranged by size:
706 524 383 248 31 7
Bpu10I CC'TnA_GC
Cuts at: 0 669 1474 1610 1899
Size: 669 805 136 289
Fragments arranged by size:
805 669 289 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 450 1899
Size: 58 392 1449
Fragments arranged by size:
1449 392 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 807 1360 1899
Size: 807 553 539
Fragments arranged by size:
807 553 539
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1373 1403 1789 1819 1899
Size: 1373 30 386 30 80
Fragments arranged by size:
1373 386 80 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 544 657 914 955 1246 1491 1537
Size: 169 375 113 257 41 291 245 46
Cuts at: 1537 1798 1899
Size: 261 101
Fragments arranged by size:
375 291 261 257 245 169 113 101
46 41
BseYI C'CCAG_C
Cuts at: 0 235 371 615 772 788 843 945 1333
Size: 235 136 244 157 16 55 102 388
Cuts at: 1333 1774 1899
Size: 441 125
Fragments arranged by size:
441 388 244 235 157 136 125 102
55 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1899
Size: 80 1819
BsiHKAI G_wGCw'C
Cuts at: 0 873 1391 1616 1718 1899
Size: 873 518 225 102 181
Fragments arranged by size:
873 518 225 181 102
BsmI GAATG_Cn'
Cuts at: 0 1813 1899
Size: 1813 86
Bsp1286I G_dGCh'C
Cuts at: 0 567 873 917 1391 1616 1718 1899
Size: 567 306 44 474 225 102 181
Fragments arranged by size:
567 474 306 225 181 102 44
BsrBI CCG'CTC
Cuts at: 0 1004 1674 1899
Size: 1004 670 225
Fragments arranged by size:
1004 670 225
BsrDI GCAATG_nn'
Cuts at: 0 1851 1899
Size: 1851 48
BstEII G'GTnAC_C
Cuts at: 0 264 1899
Size: 264 1635
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 1879 1899
Size: 132 1747 20
Fragments arranged by size:
1747 132 20
BstYI r'GATC_y
Cuts at: 0 8 953 1770 1832 1899
Size: 8 945 817 62 67
Fragments arranged by size:
945 817 67 62 8
BtgI C'CryG_G
Cuts at: 0 1553 1899
Size: 1553 346
BtsI GCAGTG_nn'
Cuts at: 0 306 887 1233 1899
Size: 306 581 346 666
Fragments arranged by size:
666 581 346 306
DraIII CAC_nnn'GTG
Cuts at: 0 171 1899
Size: 171 1728
DrdI GACnn_nn'nnGTC
Cuts at: 0 253 346 1899
Size: 253 93 1553
Fragments arranged by size:
1553 253 93
EaeI y'GGCC_r
Cuts at: 0 510 1048 1515 1899
Size: 510 538 467 384
Fragments arranged by size:
538 510 467 384
EarI CTCTTCn'nnn_
Cuts at: 0 574 727 1358 1899
Size: 574 153 631 541
Fragments arranged by size:
631 574 541 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1 7 1459 1692 1899
Size: 1 6 1452 233 207
Fragments arranged by size:
1452 233 207 6 1
EcoICRI GAG'CTC
Cuts at: 0 1614 1716 1899
Size: 1614 102 183
Fragments arranged by size:
1614 183 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 558 631 662 669 943 1375 1382
Size: 248 310 73 31 7 274 432 7
Cuts at: 1382 1899
Size: 517
Fragments arranged by size:
517 432 310 274 248 73 31 7
7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1194 1899
Size: 1194 705
EcoO109I rG'GnC_Cy
Cuts at: 0 255 477 622 1023 1116 1788 1899
Size: 255 222 145 401 93 672 111
Fragments arranged by size:
672 401 255 222 145 111 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1659 1899
Size: 1659 240
HaeII r_GCGC'y
Cuts at: 0 236 372 545 1899
Size: 236 136 173 1354
Fragments arranged by size:
1354 236 173 136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 1121 1153 1228 1260 1789 1821
Size: 466 32 623 32 75 32 529 32
Cuts at: 1821 1899
Size: 78
Fragments arranged by size:
623 529 466 78 75 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 505 1279 1899
Size: 505 774 620
Fragments arranged by size:
774 620 505
KpnI G_GTAC'C
Cuts at: 0 1203 1899
Size: 1203 696
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 722 856 1899
Size: 125 597 134 1043
Fragments arranged by size:
1043 597 134 125
MscI TGG'CCA
Cuts at: 0 512 1517 1899
Size: 512 1005 382
Fragments arranged by size:
1005 512 382
MslI CAynn'nnrTG
Cuts at: 0 130 1253 1764 1899
Size: 130 1123 511 135
Fragments arranged by size:
1123 511 135 130
MspA1I CmG'CkG
Cuts at: 0 226 637 792 1253 1262 1327 1899
Size: 226 411 155 461 9 65 572
Fragments arranged by size:
572 461 411 226 155 65 9
NspI r_CATG'y
Cuts at: 0 330 1176 1899
Size: 330 846 723
Fragments arranged by size:
846 723 330
PasI CC'CwG_GG
Cuts at: 0 1663 1899
Size: 1663 236
PfoI T'CCnGG_A
Cuts at: 0 1391 1899
Size: 1391 508
PpuMI rG'GwC_Cy
Cuts at: 0 255 622 1116 1899
Size: 255 367 494 783
Fragments arranged by size:
783 494 367 255
PspXI vC'TCGA_Gb
Cuts at: 0 1717 1899
Size: 1717 182
PstI C_TGCA'G
Cuts at: 0 37 1899
Size: 37 1862
PvuII CAG'CTG
Cuts at: 0 226 637 792 1327 1899
Size: 226 411 155 535 572
Fragments arranged by size:
572 535 411 226 155
SacI G_AGCT'C
Cuts at: 0 1616 1718 1899
Size: 1616 102 181
Fragments arranged by size:
1616 181 102
SfcI C'TryA_G
Cuts at: 0 33 139 405 1836 1899
Size: 33 106 266 1431 63
Fragments arranged by size:
1431 266 106 63 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1130 1899
Size: 1130 769
SmaI CCC'GGG
Cuts at: 0 1063 1899
Size: 1063 836
SmlI C'TyrA_G
Cuts at: 0 73 429 1717 1899
Size: 73 356 1288 182
Fragments arranged by size:
1288 356 182 73
SphI G_CATG'C
Cuts at: 0 1176 1899
Size: 1176 723
StuI AGG'CCT
Cuts at: 0 1124 1243 1899
Size: 1124 119 656
Fragments arranged by size:
1124 656 119
StyI C'CwwG_G
Cuts at: 0 107 1119 1899
Size: 107 1012 780
Fragments arranged by size:
1012 780 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1143 1899
Size: 1143 756
TatI w'GTAC_w
Cuts at: 0 1106 1899
Size: 1106 793
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1373 1405 1899
Size: 1373 32 494
Fragments arranged by size:
1373 494 32
Tth111I GACn'n_nGTC
Cuts at: 0 379 1899
Size: 379 1520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1525 1899
Size: 1525 374
XhoI C'TCGA_G
Cuts at: 0 1717 1899
Size: 1717 182
XmaI C'CCGG_G
Cuts at: 0 1061 1899
Size: 1061 838
XmnI GAAnn'nnTTC
Cuts at: 0 68 997 1899
Size: 68 929 902
Fragments arranged by size:
929 902 68
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BclI BglI BlpI BmrI
BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BsrBI BsrDI BstEII BstXI BstYI
BtgI BtsI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KpnI
MmeI MscI MslI MspA1I NspI PasI PfoI PpuMI
PspXI PstI PvuII SacI SfcI SfiI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
BaeI BbeI BcgI BciVI BfrBI BglII Bme1580I BmgBI
BmtI BplI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EagI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI
ZraI