Restriction map for
pFN21AB3002
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I AspEI AspI
BbrPI BcgI BclI BfrI BglII
BsaAI BsaBI BsaI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
HgaI HindIII HpaI KspI MamI
MfeI MluI MunI MvnI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1055 GGTACC G^GTACC
AccI 1282 GTCGAC GT^[AC][GT]AC
AccIII 1610 TCCGGA T^CCGGA
AcsI 44 AAATTC [GA]^AATT[CT]
AcyI 1460 GGCGCC G[GA]^CG[CT]C
Alw44I 722 GTGCAC G^TGCAC
AlwNI 692 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 722 GTGCAC G^TGCAC
ApoI 44 AAATTC [GA]^AATT[CT]
Asp718I 1055 GGTACC G^GTACC
AviII 146 TGCGCA TGC^GCA
AvrII 85 CCTAGG C^CTAGG
BamHI 3 GGATCC G^GATCC
BbeI 1460 GGCGCC GGCGC^C
BbvCI 1593 CCTCAGC CCTCAGC(-5/-2)
BciVI 902 GTATCC GTATCC(6/5)
BglI 1443 GCCAGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 85 CCTAGG C^CTAGG
BpmI 949 CTGGAG CTGGAG(16/14)
BsaHI 1460 GGCGCC G[GA]^CG[CT]C
BsaWI 1610 TCCGGA [AT]^CCGG[AT]
BseAI 1610 TCCGGA T^CCGGA
BspEI 1610 TCCGGA T^CCGGA
BspMI 1707 ACCTGC ACCTGC(4/8)
DraIII 244 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1620 CCATGG C^C[GA][CT]GG
EcoT22I 56 ATGCAT ATGCA^T
FspI 146 TGCGCA TGC^GCA
HaeII 1460 GGCGCC [GA]GCGC^[CT]
Hin1I 1460 GGCGCC G[GA]^CG[CT]C
HincII 1282 GTCGAC GT[CT]^[GA]AC
HindII 1282 GTCGAC GT[CT]^[GA]AC
KasI 1460 GGCGCC G^GCGCC
KpnI 1055 GGTACC GGTAC^C
MroI 1610 TCCGGA T^CCGGA
MseI 1217 TTAA T^TAA
NaeI 1430 GCCGGC GCC^GGC
NarI 1460 GGCGCC GG^CGCC
NcoI 1620 CCATGG C^CATGG
NgoMIV 1430 GCCGGC G^CCGGC
NsiI 56 ATGCAT ATGCA^T
PflMI 1016 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1575 GAGTC GAGTC(4/5)
PstI 897 CTGCAG CTGCA^G
SalI 1282 GTCGAC G^TCGAC
SapI 1008 GCTCTTC GCTCTTC(1/4)
SfoI 1460 GGCGCC GGC^GCC
SgrAI 1456 CACCGGCG C[GA]^CCGG[CT]G
TaqI 1283 TCGA T^CGA
Tru9I 1217 TTAA T^TAA
Tsp509I 45 AATT ^AATT
TthHB8I 1283 TCGA T^CGA
Van91I 1016 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 167 CCCGGG C^[CT]CG[GA]G
312 CCCGGG
BfaI 86 CTAG C^TAG
686 CTAG
BsgI 1526 CTGCAC GTGCAG(16/14)
620 GTGCAG
BsmAI 812 GAGAC GTCTC(1/5)
528 GTCTC
BsoBI 167 CCCGGG C^[CT]CG[GA]G
312 CCCGGG
BstYI 3 GGATCC [GA]^GATC[CT]
944 AGATCC
CfoI 147 GCGC GCG^C
1461 GCGC
FauI 956 GCGGG CCCGC(4/6)
1716 GCGGG
HhaI 147 GCGC GCG^C
1461 GCGC
HinP1I 147 GCGC G^CGC
1461 GCGC
MaeI 86 CTAG C^TAG
686 CTAG
MaeII 1155 ACGT A^CGT
1286 ACGT
MflI 3 GGATCC [GA]^GATC[CT]
944 AGATCC
NspI 1240 GCATGT [GA]CATG^[CT]
1587 ACATGC
SacI 123 GAGCTC GAGCT^C
1006 GAGCTC
ScaI 69 AGTACT AGT^ACT
1415 AGTACT
SmaI 167 CCCGGG CCC^GGG
312 CCCGGG
TfiI 81 GATTC G^A[AT]TC
386 GATTC
XhoII 3 GGATCC [GA]^GATC[CT]
944 AGATCC
XmaCI 167 CCCGGG C^CCGGG
312 CCCGGG
XmaI 167 CCCGGG C^CCGGG
312 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BlpI BmyI
Bpu1102I BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CelII Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TseI Tsp45I TspRI XcmI