Restriction map for
pFN21AB2389
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# NO CUT ENZYMES ( 76 enzymes) #
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Acc65I AccIII AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AviII BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrGI BssHII
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoT22I FseI
FspI HpaI KpnI MfeI MroI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1999 GTAGAC GT^[AC][GT]AC
AcsI 1960 GAATTT [GA]^AATT[CT]
AflII 147 CTTAAG C^TTAAG
Alw44I 1663 GTGCAC G^TGCAC
ApaLI 1663 GTGCAC G^TGCAC
ApoI 1960 GAATTT [GA]^AATT[CT]
AvaI 696 CCCGGG C^[CT]CG[GA]G
AvrII 6 CCTAGG C^CTAGG
BamHI 686 GGATCC G^GATCC
BbeI 3140 GGCGCC GGCGC^C
BbrPI 2812 CACGTG CAC^GTG
BbvCI 3115 GCTGAGG CCTCAGC(-5/-2)
BcgI 1169 CGAGCACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1502 GTATCC GTATCC(6/5)
BclI 209 TGATCA T^GATCA
BfrI 147 CTTAAG C^TTAAG
BlnI 6 CCTAGG C^CTAGG
BsaAI 2812 CACGTG [CT]AC^GT[GA]
BsmBI 2469 CGTCTC CGTCTC(1/5)
BsoBI 696 CCCGGG C^[CT]CG[GA]G
BspLU11I 438 ACATGT A^CATGT
BsrFI 1037 ACCGGC [GA]^CCGG[CT]
BssSI 3088 CACGAG CACGAG(-5/-1)
BstEII 514 GGTCACC G^GT[ACGT]ACC
BstPI 514 GGTCACC G^GT[ACGT]ACC
Cfr10I 1037 ACCGGC [GA]^CCGG[CT]
DraIII 241 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 514 GGTCACC G^GT[ACGT]ACC
EcoRV 1201 GATATC GAT^ATC
FbaI 209 TGATCA T^GATCA
HincII 448 GTTGAC GT[CT]^[GA]AC
HindII 448 GTTGAC GT[CT]^[GA]AC
KasI 3140 GGCGCC G^GCGCC
KspI 660 CCGCGG CCGC^GG
NarI 3140 GGCGCC GG^CGCC
NcoI 1220 CCATGG C^CATGG
NdeI 1252 CATATG CA^TATG
PmaCI 2812 CACGTG CAC^GTG
PmlI 2812 CACGTG CAC^GTG
PshAI 2002 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 660 CCGCGG CCGC^GG
SfiI 1638 GGCCTCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3140 GGCGCC GGC^GCC
SmaI 696 CCCGGG CCC^GGG
StuI 143 AGGCCT AGG^CCT
XbaI 2031 TCTAGA T^CTAGA
XmaCI 696 CCCGGG C^CCGGG
XmaI 696 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AatII 484 GACGTC GACGT^C
1387 GACGTC
AfaI 564 GTAC GT^AC
2424 GTAC
Aor51HI 425 AGCGCT AGC^GCT
3095 AGCGCT
ApaI 693 GGGCCC GGGCC^C
1334 GGGCCC
Asp700I 2322 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2674 GAATATATTC
AspI 249 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2854 GACTCAGTC
BglI 721 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1639 GCCTCCGTGGC
BglII 155 AGATCT A^GATCT
2375 AGATCT
Eco47III 425 AGCGCT AGC^GCT
3095 AGCGCT
HindIII 359 AAGCTT A^AGCTT
2383 AAGCTT
MluI 623 ACGCGT A^CGCGT
2042 ACGCGT
NspI 438 ACATGT [GA]CATG^[CT]
734 ACATGC
PflFI 249 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2854 GACTCAGTC
PflMI 2106 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2190 CCATATCCTGG
RsaI 564 GTAC GT^AC
2424 GTAC
SacI 1876 GAGCTC GAGCT^C
2200 GAGCTC
SapI 2322 GAAGAGC GCTCTTC(1/4)
1930 GCTCTTC
SfaNI 2493 GATGC GCATC(5/9)
2480 GCATC
TaqI 338 TCGA T^CGA
2333 TCGA
Tth111I 249 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2854 GACTCAGTC
TthHB8I 338 TCGA T^CGA
2333 TCGA
Van91I 2106 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2190 CCATATCCTGG
XcmI 635 CCATGCCCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2627 CCAGGAGACTGCTGG
XmnI 2322 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2674 GAATATATTC
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AlwI AlwNI AocI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII