Restriction map for
pFN21AB2389
(Linear) (Six-base) MAPSORT of: KIBB2389.seq Check: 9475 from: 1 to: 3174
KIBB2389.orf
With 182 enzymes: SgfI *
August 12, 2008 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 974 1110 1422 3037 3174
Size: 974 136 312 1615 137
Fragments arranged by size:
1615 974 312 137 136
AatII G_ACGT'C
Cuts at: 0 488 1391 3174
Size: 488 903 1783
Fragments arranged by size:
1783 903 488
AccI GT'mk_AC
Cuts at: 0 2000 3174
Size: 2000 1174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1047 1201 1975 2079 2106 2341 2725 3152
Size: 1047 154 774 104 27 235 384 427
Cuts at: 3152 3174
Size: 22
Fragments arranged by size:
1047 774 427 384 235 154 104 27
22
AfeI AGC'GCT
Cuts at: 0 427 3097 3174
Size: 427 2670 77
Fragments arranged by size:
2670 427 77
AflII C'TTAA_G
Cuts at: 0 147 3174
Size: 147 3027
AflIII A'CryG_T
Cuts at: 0 120 438 623 2042 3174
Size: 120 318 185 1419 1132
Fragments arranged by size:
1419 1132 318 185 120
AleI CACnn'nnGTG
Cuts at: 0 2941 3174
Size: 2941 233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2061 3174
Size: 2061 1113
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 3174
Size: 271 32 2871
Fragments arranged by size:
2871 271 32
AlwNI CAG_nnn'CTG
Cuts at: 0 384 864 1927 2910 3174
Size: 384 480 1063 983 264
Fragments arranged by size:
1063 983 480 384 264
ApaI G_GGCC'C
Cuts at: 0 697 1338 3174
Size: 697 641 1836
Fragments arranged by size:
1836 697 641
ApaLI G'TGCA_C
Cuts at: 0 1663 3174
Size: 1663 1511
ApoI r'AATT_y
Cuts at: 0 1960 3174
Size: 1960 1214
AvaI C'yCGr_G
Cuts at: 0 696 3174
Size: 696 2478
AvrII C'CTAG_G
Cuts at: 0 6 3174
Size: 6 3168
BamHI G'GATC_C
Cuts at: 0 686 3174
Size: 686 2488
BanI G'GyrC_C
Cuts at: 0 1717 2156 3140 3174
Size: 1717 439 984 34
Fragments arranged by size:
1717 984 439 34
BanII G_rGCy'C
Cuts at: 0 697 1338 1880 2204 2444 3174
Size: 697 641 542 324 240 730
Fragments arranged by size:
730 697 641 542 324 240
BbeI G_GCGC'C
Cuts at: 0 3144 3174
Size: 3144 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 470 1577 2399 3174
Size: 287 183 1107 822 775
Fragments arranged by size:
1107 822 775 287 183
BbvCI CC'TCA_GC
Cuts at: 0 3116 3174
Size: 3116 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1158 1192 3174
Size: 1158 34 1982
Fragments arranged by size:
1982 1158 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1513 3174
Size: 1513 1661
BclI T'GATC_A
Cuts at: 0 209 3174
Size: 209 2965
BglI GCCn_nnn'nGGC
Cuts at: 0 727 1645 3174
Size: 727 918 1529
Fragments arranged by size:
1529 918 727
BglII A'GATC_T
Cuts at: 0 155 2375 3174
Size: 155 2220 799
Fragments arranged by size:
2220 799 155
Bme1580I G_kGCm'C
Cuts at: 0 631 697 1338 1667 2342 3087 3174
Size: 631 66 641 329 675 745 87
Fragments arranged by size:
745 675 641 631 329 87 66
BmgBI CAC'GTC
Cuts at: 0 468 2517 3174
Size: 468 2049 657
Fragments arranged by size:
2049 657 468
BmrI ACTGGGnnnn_n'
Cuts at: 0 123 534 920 3174
Size: 123 411 386 2254
Fragments arranged by size:
2254 411 386 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 177 2256 2421 3174
Size: 177 2079 165 753
Fragments arranged by size:
2079 753 177 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2050 2218 2457 2659 3061 3174
Size: 2050 168 239 202 402 113
Fragments arranged by size:
2050 402 239 202 168 113
Bpu10I CC'TnA_GC
Cuts at: 0 607 3116 3174
Size: 607 2509 58
Fragments arranged by size:
2509 607 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1957 1998 3174
Size: 1957 41 1176
Fragments arranged by size:
1957 1176 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 925 1131 2227 3174
Size: 925 206 1096 947
Fragments arranged by size:
1096 947 925 206
BsaAI yAC'GTr
Cuts at: 0 2814 3174
Size: 2814 360
BsaBI GATnn'nnATC
Cuts at: 0 214 2020 2191 3174
Size: 214 1806 171 983
Fragments arranged by size:
1806 983 214 171
BsaHI Gr'CG_yC
Cuts at: 0 485 1388 3141 3174
Size: 485 903 1753 33
Fragments arranged by size:
1753 903 485 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2140 2170 2191 2221 3174
Size: 2140 30 21 30 953
Fragments arranged by size:
2140 953 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 150 1000 2080 2545 2641 2815 3174
Size: 150 850 1080 465 96 174 359
Fragments arranged by size:
1080 850 465 359 174 150 96
BseYI C'CCAG_C
Cuts at: 0 179 3174
Size: 179 2995
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1713 1798 2610 2944 3174
Size: 943 770 85 812 334 230
Fragments arranged by size:
943 812 770 334 230 85
BsiHKAI G_wGCw'C
Cuts at: 0 1004 1174 1667 1880 2204 3174
Size: 1004 170 493 213 324 970
Fragments arranged by size:
1004 970 493 324 213 170
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2475 3174
Size: 2475 699
Bsp1286I G_dGCh'C
Cuts at: 0 631 697 1004 1174 1338 1667 1880 2204
Size: 631 66 307 170 164 329 213 324
Cuts at: 2204 2342 2444 3087 3174
Size: 138 102 643 87
Fragments arranged by size:
643 631 329 324 307 213 170 164
138 102 87 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 575 974 1110 1422 1489 2080 3037 3174
Size: 575 399 136 312 67 591 957 137
Fragments arranged by size:
957 591 575 399 312 137 136 67
BsrDI GCAATG_nn'
Cuts at: 0 557 1214 3110 3174
Size: 557 657 1896 64
Fragments arranged by size:
1896 657 557 64
BsrFI r'CCGG_y
Cuts at: 0 1037 3174
Size: 1037 2137
BssSI C'ACGA_G
Cuts at: 0 3088 3174
Size: 3088 86
BstAPI GCAn_nnn'nTGC
Cuts at: 0 966 999 2580 3174
Size: 966 33 1581 594
Fragments arranged by size:
1581 966 594 33
BstEII G'GTnAC_C
Cuts at: 0 514 3174
Size: 514 2660
BstXI CCAn_nnnn'nTGG
Cuts at: 0 942 1221 1806 3174
Size: 942 279 585 1368
Fragments arranged by size:
1368 942 585 279
BstYI r'GATC_y
Cuts at: 0 155 674 686 1424 2015 2178 2375 3174
Size: 155 519 12 738 591 163 197 799
Fragments arranged by size:
799 738 591 519 197 163 155 12
Bsu36I CC'TnA_GG
Cuts at: 0 83 1159 1762 2314 2528 2597 2746 3174
Size: 83 1076 603 552 214 69 149 428
Fragments arranged by size:
1076 603 552 428 214 149 83 69
BtgI C'CryG_G
Cuts at: 0 660 809 1220 1643 3174
Size: 660 149 411 423 1531
Fragments arranged by size:
1531 660 423 411 149
BtsI GCAGTG_nn'
Cuts at: 0 1745 3174
Size: 1745 1429
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1616 1651 2352 2387 3174
Size: 1616 35 701 35 787
Fragments arranged by size:
1616 787 701 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 246 3174
Size: 246 2928
EaeI y'GGCC_r
Cuts at: 0 62 647 719 806 914 1223 2969 3174
Size: 62 585 72 87 108 309 1746 205
Fragments arranged by size:
1746 585 309 205 108 87 72 62
EarI CTCTTCn'nnn_
Cuts at: 0 340 1345 1937 1988 2098 2317 2746 3174
Size: 340 1005 592 51 110 219 429 428
Fragments arranged by size:
1005 592 429 428 340 219 110 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1153 3174
Size: 1153 2021
EcoICRI GAG'CTC
Cuts at: 0 1878 2202 3174
Size: 1878 324 972
Fragments arranged by size:
1878 972 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1047 1201 1975 2050 2079 2106 2218 2341
Size: 1047 154 774 75 29 27 112 123
Cuts at: 2341 2457 2659 2725 3061 3152 3174
Size: 116 202 66 336 91 22
Fragments arranged by size:
1047 774 336 202 154 123 116 112
91 75 66 29 27 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1105 2798 2914 3174
Size: 1105 1693 116 260
Fragments arranged by size:
1693 1105 260 116
EcoO109I rG'GnC_Cy
Cuts at: 0 10 525 1190 1334 1335 1527 1783 2907
Size: 10 515 665 144 1 192 256 1124
Cuts at: 2907 3174
Size: 267
Fragments arranged by size:
1124 665 515 267 256 192 144 10
1
EcoRV GAT'ATC
Cuts at: 0 1203 3174
Size: 1203 1971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1311 2346 3174
Size: 1311 1035 828
Fragments arranged by size:
1311 1035 828
HaeII r_GCGC'y
Cuts at: 0 429 865 2613 3099 3144 3174
Size: 429 436 1748 486 45 30
Fragments arranged by size:
1748 486 436 429 45 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 359 391 666 698 2203 2235
Size: 119 32 208 32 275 32 1505 32
Cuts at: 2235 2484 2516 3174
Size: 249 32 658
Fragments arranged by size:
1505 658 275 249 208 119 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 450 3174
Size: 450 2724
HindIII A'AGCT_T
Cuts at: 0 359 2383 3174
Size: 359 2024 791
Fragments arranged by size:
2024 791 359
KasI G'GCGC_C
Cuts at: 0 3140 3174
Size: 3140 34
MluI A'CGCG_T
Cuts at: 0 623 2042 3174
Size: 623 1419 1132
Fragments arranged by size:
1419 1132 623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 281 664 3174
Size: 105 176 383 2510
Fragments arranged by size:
2510 383 176 105
MscI TGG'CCA
Cuts at: 0 649 721 808 916 2971 3174
Size: 649 72 87 108 2055 203
Fragments arranged by size:
2055 649 203 108 87 72
MslI CAynn'nnrTG
Cuts at: 0 940 1219 1804 2941 2971 3174
Size: 940 279 585 1137 30 203
Fragments arranged by size:
1137 940 585 279 203 30
MspA1I CmG'CkG
Cuts at: 0 72 662 1399 2477 2622 2878 3174
Size: 72 590 737 1078 145 256 296
Fragments arranged by size:
1078 737 590 296 256 145 72
NarI GG'CG_CC
Cuts at: 0 3141 3174
Size: 3141 33
NcoI C'CATG_G
Cuts at: 0 1220 3174
Size: 1220 1954
NdeI CA'TA_TG
Cuts at: 0 1253 3174
Size: 1253 1921
NspI r_CATG'y
Cuts at: 0 442 738 3174
Size: 442 296 2436
Fragments arranged by size:
2436 442 296
PasI CC'CwG_GG
Cuts at: 0 2419 3174
Size: 2419 755
PciI A'CATG_T
Cuts at: 0 438 3174
Size: 438 2736
PflMI CCAn_nnn'nTGG
Cuts at: 0 2112 2196 3174
Size: 2112 84 978
Fragments arranged by size:
2112 978 84
PfoI T'CCnGG_A
Cuts at: 0 479 1448 2195 2849 3174
Size: 479 969 747 654 325
Fragments arranged by size:
969 747 654 479 325
PmlI CAC'GTG
Cuts at: 0 2814 3174
Size: 2814 360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 3174
Size: 58 32 3084
Fragments arranged by size:
3084 58 32
PpuMI rG'GwC_Cy
Cuts at: 0 10 525 1783 2907 3174
Size: 10 515 1258 1124 267
Fragments arranged by size:
1258 1124 515 267 10
PshAI GACnn'nnGTC
Cuts at: 0 2006 3174
Size: 2006 1168
PspOMI G'GGCC_C
Cuts at: 0 693 1334 3174
Size: 693 641 1840
Fragments arranged by size:
1840 693 641
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1494 1526 3174
Size: 1494 32 1648
Fragments arranged by size:
1648 1494 32
PstI C_TGCA'G
Cuts at: 0 171 236 2770 3174
Size: 171 65 2534 404
Fragments arranged by size:
2534 404 171 65
PvuII CAG'CTG
Cuts at: 0 72 1399 2622 2878 3174
Size: 72 1327 1223 256 296
Fragments arranged by size:
1327 1223 296 256 72
SacI G_AGCT'C
Cuts at: 0 1880 2204 3174
Size: 1880 324 970
Fragments arranged by size:
1880 970 324
SacII CC_GC'GG
Cuts at: 0 663 3174
Size: 663 2511
SanDI GG'GwC_CC
Cuts at: 0 1783 3174
Size: 1783 1391
SapI GCTCTTCn'nnn_
Cuts at: 0 1937 2317 3174
Size: 1937 380 857
Fragments arranged by size:
1937 857 380
SfcI C'TryA_G
Cuts at: 0 167 232 2766 3174
Size: 167 65 2534 408
Fragments arranged by size:
2534 408 167 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1645 3174
Size: 1645 1529
SfoI GGC'GCC
Cuts at: 0 3142 3174
Size: 3142 32
SmaI CCC'GGG
Cuts at: 0 698 3174
Size: 698 2476
SmlI C'TyrA_G
Cuts at: 0 147 1936 1977 3174
Size: 147 1789 41 1197
Fragments arranged by size:
1789 1197 147 41
StuI AGG'CCT
Cuts at: 0 145 3174
Size: 145 3029
StyI C'CwwG_G
Cuts at: 0 6 224 1106 1193 1220 1853 1911 2144
Size: 6 218 882 87 27 633 58 233
Cuts at: 2144 2584 2884 3014 3174
Size: 440 300 130 160
Fragments arranged by size:
882 633 440 300 233 218 160 130
87 58 27 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 853 1735 1866 2432 2953 3174
Size: 853 882 131 566 521 221
Fragments arranged by size:
882 853 566 521 221 131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 696 728 828 860 2140 2172
Size: 475 32 189 32 100 32 1280 32
Cuts at: 2172 2191 2223 3174
Size: 19 32 951
Fragments arranged by size:
1280 951 475 189 100 32 32 32
32 32 19
Tth111I GACn'n_nGTC
Cuts at: 0 252 2857 3174
Size: 252 2605 317
Fragments arranged by size:
2605 317 252
XbaI T'CTAG_A
Cuts at: 0 2031 3174
Size: 2031 1143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 642 2634 3174
Size: 642 1992 540
Fragments arranged by size:
1992 642 540
XmaI C'CCGG_G
Cuts at: 0 696 3174
Size: 696 2478
XmnI GAAnn'nnTTC
Cuts at: 0 2326 2678 3174
Size: 2326 352 496
Fragments arranged by size:
2326 496 352
ZraI GAC'GTC
Cuts at: 0 486 1389 3174
Size: 486 903 1785
Fragments arranged by size:
1785 903 486
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuII SacI SacII SanDI
SapI SfcI SfiI SfoI SmaI SmlI StuI StyI
TaqII TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AhdI AscI AseI AsiSI BaeI
BfrBI BlpI BmtI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrGI BssHII BstBI BstZ17I BtgZI ClaI
DraI DrdI EagI EcoRI FseI FspI FspAI HpaI
KpnI MfeI NaeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PsiI PspXI PvuI RsrII SalI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI XhoI