Restriction map for
pFN21AB1487
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# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbeI
BfrI BlnI BsaHI BseAI BsiEI
BspDI BspEI BssSI ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FspI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NotI NsiI PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SacII
SalI ScaI SexAI SfoI SgrAI
SmaI SnaBI SpeI SspI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 309 GTATAC GT^[AC][GT]AC
AclI 5014 AACGTT AA^CGTT
Aor51HI 2098 AGCGCT AGC^GCT
ApaI 3061 GGGCCC GGGCC^C
Asp700I 4476 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 57 GGATCC G^GATCC
BbrPI 693 CACGTG CAC^GTG
BcgI 535 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4895 TGATCA T^GATCA
BlpI 1696 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1696 GCTGAGC GC^T[ACGT]AGC
BsiWI 1608 CGTACG C^GTACG
BspHI 4535 TCATGA T^CATGA
BspLU11I 4928 ACATGT A^CATGT
BsrBI 5338 CCGCTC CCGCTC(-3/-3)
BssHII 3898 GCGCGC G^CGCGC
Bst1107I 309 GTATAC GTA^TAC
BstBI 154 TTCGAA TT^CGAA
BstZ17I 309 GTATAC GTA^TAC
CelII 1696 GCTGAGC GC^T[ACGT]AGC
Eco47III 2098 AGCGCT AGC^GCT
FbaI 4895 TGATCA T^GATCA
FseI 556 GGCCGGCC GGCCGG^CC
HindIII 5378 AAGCTT A^AGCTT
NdeI 4067 CATATG CA^TATG
NheI 3184 GCTAGC G^CTAGC
NruI 5442 TCGCGA TCG^CGA
NspV 154 TTCGAA TT^CGAA
PmaCI 693 CACGTG CAC^GTG
PmlI 693 CACGTG CAC^GTG
Psp1406I 5014 AACGTT AA^CGTT
RcaI 4535 TCATGA T^CATGA
SapI 4285 GAAGAGC GCTCTTC(1/4)
SfiI 2549 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 154 TTCGAA TT^CGAA
XmnI 4476 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AspI 2808 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4044 GACCCTGTC
AvaI 4333 CCCGAG C^[CT]CG[GA]G
5509 CCCGAG
BciVI 5497 GGATAC GTATCC(6/5)
2042 GTATCC
BsaBI 4406 GATTAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4527 GATTAGCATC
BsmBI 3462 CGTCTC CGTCTC(1/5)
2632 GAGACG
BsoBI 4333 CCCGAG C^[CT]CG[GA]G
5509 CCCGAG
Bsp1407I 689 TGTACA T^GTACA
4086 TGTACA
BsrGI 689 TGTACA T^GTACA
4086 TGTACA
DraIII 4010 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5130 CACGCAGTG
HaeII 1783 AGCGCC [GA]GCGC^[CT]
2098 AGCGCT
HgaI 3393 GACGC GACGC(5/10)
3523 GACGC
MamI 4406 GATTAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4527 GATTAGCATC
MseI 3212 TTAA T^TAA
5596 TTAA
PflFI 2808 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4044 GACCCTGTC
PshAI 1002 GACTCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4813 GACTTTGGTC
SbfI 1038 CCTGCAGG CCTGCA^GG
1875 CCTGCAGG
SphI 641 GCATGC GCATG^C
2361 GCATGC
Sse8387I 1038 CCTGCAGG CCTGCA^GG
1875 CCTGCAGG
SspBI 689 TGTACA T^GTACA
4086 TGTACA
Tru9I 3212 TTAA T^TAA
5596 TTAA
Tth111I 2808 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4044 GACCCTGTC
XcmI 746 CCATCCAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4595 CCAACATCGTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII