(Circular) (Six-base) MAPSORT of: pFN21AB1487.seq Check: 6339 from: 1 to: 10444 pFN21AB1487 10444 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3692 3758 3766 4094 7652 7685 3692 Size: 66 8 328 3558 33 6451 Fragments arranged by size: 6451 3558 328 66 33 8 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8254 278 Size: 53 83 186 936 6718 2468 Fragments arranged by size: 6718 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2315 7787 1937 Size: 378 5472 4594 Fragments arranged by size: 5472 4594 378 Acc65I G'GTAC_C Cuts at: 7765 7765 Size: 10444 AclI AA'CG_TT Cuts at: 1563 7020 8571 8944 1563 Size: 5457 1551 373 3063 Fragments arranged by size: 5457 3063 1551 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2427 3002 3354 3584 3981 4301 4643 4769 Size: 795 575 352 230 397 320 342 126 Cuts at: 4769 5046 5129 5165 6309 6878 6915 7124 7130 Size: 277 83 36 1144 569 37 209 6 Cuts at: 7130 7389 8507 9891 10159 1632 Size: 259 1118 1384 268 1917 Fragments arranged by size: 1917 1384 1144 1118 795 575 569 397 352 342 320 277 268 259 230 209 126 83 37 36 6 AfeI AGC'GCT Cuts at: 4105 4105 Size: 10444 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10247 AflIII A'CryG_T Cuts at: 1203 2697 4329 6228 6933 9343 10320 1203 Size: 1494 1632 1899 705 2410 977 1327 Fragments arranged by size: 2410 1899 1632 1494 1327 977 705 AgeI A'CCGG_T Cuts at: 9275 9275 Size: 10444 AhdI GACnn_n'nnGTC Cuts at: 9173 9173 Size: 10444 AleI CACnn'nnGTG Cuts at: 1166 3362 5984 1166 Size: 2196 2622 5626 Fragments arranged by size: 5626 2622 2196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2631 5363 1184 Size: 1447 2732 6265 Fragments arranged by size: 6265 2732 1447 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2736 2768 5168 5200 2736 Size: 32 2400 32 7980 Fragments arranged by size: 7980 2400 32 32 AlwNI CAG_nnn'CTG Cuts at: 2656 3792 5314 6184 8187 9759 2656 Size: 1136 1522 870 2003 1572 3341 Fragments arranged by size: 3341 2003 1572 1522 1136 870 ApaI G_GGCC'C Cuts at: 5070 5070 Size: 10444 ApaLI G'TGCA_C Cuts at: 4259 4838 6749 8501 9657 4259 Size: 579 1911 1752 1156 5046 Fragments arranged by size: 5046 1911 1752 1156 579 ApoI r'AATT_y Cuts at: 1493 3248 6425 6881 7753 7979 8131 10240 1493 Size: 1755 3177 456 872 226 152 2109 1697 Fragments arranged by size: 3177 2109 1755 1697 872 456 226 152 AseI AT'TA_AT Cuts at: 160 1032 8998 160 Size: 872 7966 1606 Fragments arranged by size: 7966 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10444 AvaI C'yCGr_G Cuts at: 1870 1943 6338 7514 7769 1870 Size: 73 4395 1176 255 4545 Fragments arranged by size: 4545 4395 1176 255 73 BamHI G'GATC_C Cuts at: 2062 7774 8141 2062 Size: 5712 367 4365 Fragments arranged by size: 5712 4365 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3356 3740 4157 4915 6797 Size: 325 217 627 1569 384 417 758 1882 Cuts at: 6797 6872 7765 9220 618 Size: 75 893 1455 1842 Fragments arranged by size: 1882 1842 1569 1455 893 758 627 417 384 325 217 75 BanII G_rGCy'C Cuts at: 1391 2190 3708 4459 5070 5630 6041 6339 6915 Size: 799 1518 751 611 560 411 298 576 Cuts at: 6915 7305 7692 7763 1391 Size: 390 387 71 4072 Fragments arranged by size: 4072 1518 799 751 611 576 560 411 390 387 298 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3029 4602 4746 5361 7279 928 Size: 971 1130 1573 144 615 1918 4093 Fragments arranged by size: 4093 1918 1573 1130 971 615 144 BbvCI CC'TCA_GC Cuts at: 2946 7027 7404 2946 Size: 4081 377 5986 Fragments arranged by size: 5986 4081 377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2529 2563 8635 8669 1903 Size: 34 592 34 6072 34 3678 Fragments arranged by size: 6072 3678 592 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4058 7496 8336 9546 4058 Size: 3438 840 1210 4956 Fragments arranged by size: 4956 3438 1210 840 BclI T'GATC_A Cuts at: 1548 6900 1548 Size: 5352 5092 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3698 4561 5065 Size: 107 122 71 631 605 2026 863 504 Cuts at: 5065 9055 136 Size: 3990 1525 Fragments arranged by size: 3990 2026 1525 863 631 605 504 122 107 71 BglII A'GATC_T Cuts at: 6111 6708 7065 10439 6111 Size: 597 357 3374 6116 Fragments arranged by size: 6116 3374 597 357 BlpI GC'TnA_GC Cuts at: 3702 7861 3702 Size: 4159 6285 Bme1580I G_kGCm'C Cuts at: 1790 2483 2620 3633 4079 4263 4842 5070 6753 Size: 693 137 1013 446 184 579 228 1683 Cuts at: 6753 6800 8505 9661 1790 Size: 47 1705 1156 2573 Fragments arranged by size: 2573 1705 1683 1156 1013 693 579 446 228 184 137 47 BmgBI CAC'GTC Cuts at: 1318 2686 4330 4609 6135 1318 Size: 1368 1644 279 1526 5627 Fragments arranged by size: 5627 1644 1526 1368 279 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3620 8175 9133 Size: 416 53 83 399 24 2203 4555 958 Cuts at: 9133 442 Size: 1753 Fragments arranged by size: 4555 2203 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5193 1056 Size: 4137 6307 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3144 4047 3144 Size: 903 9541 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2881 2888 2990 3126 3538 4128 5339 5559 Size: 1642 7 102 136 412 590 1211 220 Cuts at: 5559 6638 6860 9104 1239 Size: 1079 222 2244 2579 Fragments arranged by size: 2579 2244 1642 1211 1079 590 412 222 220 136 102 7 Bpu10I CC'TnA_GC Cuts at: 2946 5045 6561 7027 7404 2946 Size: 2099 1516 466 377 5986 Fragments arranged by size: 5986 2099 1516 466 377 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2053 2573 4376 5189 5402 6746 7412 8569 9434 Size: 520 1803 813 213 1344 666 1157 865 Cuts at: 9434 9732 2053 Size: 298 2765 Fragments arranged by size: 2765 1803 1344 1157 865 813 666 520 298 213 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2447 4692 5000 5096 5132 6858 9107 Size: 617 948 2245 308 96 36 1726 2249 Cuts at: 9107 882 Size: 2219 Fragments arranged by size: 2249 2245 2219 1726 948 617 308 96 36 BsaAI yAC'GTr Cuts at: 493 1204 2700 4080 6218 8235 493 Size: 711 1496 1380 2138 2017 2702 Fragments arranged by size: 2702 2138 2017 1496 1380 711 BsaBI GATnn'nnATC Cuts at: 6415 6536 10438 6415 Size: 121 3902 6421 Fragments arranged by size: 6421 3902 121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 8251 8633 9268 275 Size: 53 83 186 936 6718 382 635 1451 Fragments arranged by size: 6718 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3010 6351 7206 8877 9275 9549 9696 10107 3010 Size: 3341 855 1671 398 274 147 411 3347 Fragments arranged by size: 3347 3341 1671 855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3662 3692 5751 5781 6611 Size: 30 165 30 2259 30 2059 30 830 Cuts at: 6611 6641 6671 6701 1178 Size: 30 30 30 4921 Fragments arranged by size: 4921 2259 2059 830 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2312 3120 3382 4521 4604 4670 6031 6058 Size: 1126 808 262 1139 83 66 1361 27 Cuts at: 6058 6419 1186 Size: 361 5211 Fragments arranged by size: 5211 1361 1139 1126 808 361 262 83 66 27 BseYI C'CCAG_C Cuts at: 2503 3854 4161 4759 4848 4957 5296 5380 5950 Size: 1351 307 598 89 109 339 84 570 Cuts at: 5950 6004 9647 10143 2503 Size: 54 3643 496 2804 Fragments arranged by size: 3643 2804 1351 598 570 496 339 307 109 89 84 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3448 3852 3959 4376 7117 7663 1163 Size: 573 1712 404 107 417 2741 546 3944 Fragments arranged by size: 3944 2741 1712 573 546 417 404 107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7894 8655 9683 1540 Size: 275 186 5893 761 1028 2301 Fragments arranged by size: 5893 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2347 3079 3611 3708 4134 4263 4842 5549 5630 Size: 732 532 97 426 129 579 707 81 Cuts at: 5630 5853 5916 6041 6753 7692 8505 8590 9661 Size: 223 63 125 712 939 813 85 1071 Cuts at: 9661 2347 Size: 3130 Fragments arranged by size: 3130 1071 939 813 732 712 707 579 532 426 223 129 125 97 85 81 63 BsiWI C'GTAC_G Cuts at: 3613 3613 Size: 10444 BsmI GAATG_Cn' Cuts at: 3239 3769 4415 5020 6850 7055 7955 8048 3239 Size: 530 646 605 1830 205 900 93 5635 Fragments arranged by size: 5635 1830 900 646 605 530 205 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4631 5473 4631 Size: 842 9602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2347 2483 2620 3079 3611 3633 Size: 399 400 157 136 137 459 532 22 Cuts at: 3633 3708 4079 4134 4263 4459 4842 5070 5549 Size: 75 371 55 129 196 383 228 479 Cuts at: 5549 5630 5853 5916 6041 6339 6753 6800 6915 Size: 81 223 63 125 298 414 47 115 Cuts at: 6915 7305 7692 7763 8505 8590 9661 1391 Size: 390 387 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 532 479 459 414 400 399 390 387 383 371 298 228 223 196 157 137 136 129 125 115 85 81 75 71 63 55 47 22 BspEI T'CCGG_A Cuts at: 10107 10107 Size: 10444 BspHI T'CATG_A Cuts at: 6540 8333 10063 6540 Size: 1793 1730 6921 Fragments arranged by size: 6921 1793 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2566 2641 3692 3758 3766 4094 4797 4843 Size: 1722 75 1051 66 8 328 703 46 Cuts at: 4843 4939 5689 6192 7652 7685 7799 844 Size: 96 750 503 1460 33 114 3489 Fragments arranged by size: 3489 1722 1460 1051 750 703 503 328 114 96 75 66 46 33 8 BsrBI CCG'CTC Cuts at: 1394 1823 7345 8331 1394 Size: 429 5522 986 3507 Fragments arranged by size: 5522 3507 986 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5056 5164 5715 7258 7296 8939 9113 Size: 1183 3808 108 551 1543 38 1643 174 Cuts at: 9113 65 Size: 1396 Fragments arranged by size: 3808 1643 1543 1396 1183 551 174 108 38 BsrFI r'CCGG_y Cuts at: 1700 1810 2562 3692 4370 9088 9275 1700 Size: 110 752 1130 678 4718 187 2869 Fragments arranged by size: 4718 2869 1130 752 678 187 110 BsrGI T'GTAC_A Cuts at: 96 2694 6091 96 Size: 2598 3397 4449 Fragments arranged by size: 4449 3397 2598 BssHII G'CGCG_C Cuts at: 1925 5903 1925 Size: 3978 6466 BssSI C'ACGA_G Cuts at: 8504 9516 8504 Size: 1012 9432 BstAPI GCAn_nnn'nTGC Cuts at: 794 2892 5314 7140 794 Size: 2098 2422 1826 4098 Fragments arranged by size: 4098 2422 2098 1826 BstBI TT'CG_AA Cuts at: 2160 9248 2160 Size: 7088 3356 BstEII G'GTnAC_C Cuts at: 1184 2177 2578 2827 3796 4241 7329 1184 Size: 993 401 249 969 445 3088 4299 Fragments arranged by size: 4299 3088 993 969 445 401 249 BstXI CCAn_nnnn'nTGG Cuts at: 2912 5026 6838 7180 2912 Size: 2114 1812 342 6176 Fragments arranged by size: 6176 2114 1812 342 BstYI r'GATC_y Cuts at: 1972 2062 3990 4383 4755 5835 6111 6416 6708 Size: 90 1928 393 372 1080 276 305 292 Cuts at: 6708 7065 7774 8141 8526 8543 9995 10081 10093 Size: 357 709 367 385 17 1452 86 12 Cuts at: 10093 10439 1972 Size: 346 1977 Fragments arranged by size: 1977 1928 1452 1080 709 393 385 372 367 357 346 305 292 276 90 86 17 12 BstZ17I GTA'TAC Cuts at: 2316 2316 Size: 10444 Bsu36I CC'TnA_GG Cuts at: 2422 4550 5490 5736 2422 Size: 2128 940 246 7130 Fragments arranged by size: 7130 2128 940 246 BtgI C'CryG_G Cuts at: 513 1065 2004 2665 3216 3736 4463 5079 5151 Size: 552 939 661 551 520 727 616 72 Cuts at: 5151 5562 7154 513 Size: 411 1592 3803 Fragments arranged by size: 3803 1592 939 727 661 616 552 551 520 411 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9705 Fragments arranged by size: 9705 683 56 BtsI GCAGTG_nn' Cuts at: 2344 2454 2603 3465 3611 3864 5005 5031 5483 Size: 110 149 862 146 253 1141 26 452 Cuts at: 5483 5914 5930 6091 7145 7960 8754 8774 2344 Size: 431 16 161 1054 815 794 20 4014 Fragments arranged by size: 4014 1141 1054 862 815 794 452 431 253 161 149 146 110 26 20 16 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2320 2355 529 Size: 35 1756 35 8618 Fragments arranged by size: 8618 1756 35 35 DraI TTT'AAA Cuts at: 7748 7748 Size: 10444 DraIII CAC_nnn'GTG Cuts at: 6020 7140 6020 Size: 1120 9324 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9451 809 Size: 507 8135 1802 Fragments arranged by size: 8135 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2560 4300 4372 Size: 54 1425 50 275 18 730 1740 72 Cuts at: 4372 4562 4875 5533 5898 6828 7014 7891 8780 Size: 190 313 658 365 930 186 877 889 Cuts at: 8780 10409 8 Size: 1629 43 Fragments arranged by size: 1740 1629 1425 930 889 877 730 658 365 313 275 190 186 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7891 1537 Size: 275 6079 4090 Fragments arranged by size: 6079 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3105 3113 3809 3821 4619 4821 5637 6285 Size: 1752 8 696 12 798 202 816 648 Cuts at: 6285 7725 7734 8374 1353 Size: 1440 9 640 3423 Fragments arranged by size: 3423 1752 1440 816 798 696 648 640 202 12 9 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4655 5555 9031 9405 9551 387 Size: 1064 138 3066 900 3476 374 146 1280 Fragments arranged by size: 3476 3066 1280 1064 900 374 146 138 EcoICRI GAG'CTC Cuts at: 3706 5628 6039 7690 3706 Size: 1922 411 1651 6460 Fragments arranged by size: 6460 1922 1651 411 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2427 2881 2888 2990 3002 3126 3354 Size: 393 795 454 7 102 12 124 228 Cuts at: 3354 3538 3584 3981 4128 4301 4643 4769 5046 Size: 184 46 397 147 173 342 126 277 Cuts at: 5046 5129 5165 5339 5559 6309 6638 6860 6878 Size: 83 36 174 220 750 329 222 18 Cuts at: 6878 6915 7124 7130 7389 8507 9104 9891 10159 Size: 37 209 6 259 1118 597 787 268 Cuts at: 10159 1239 Size: 1524 Fragments arranged by size: 1524 1118 795 787 750 597 454 397 393 342 329 277 268 259 228 222 220 209 184 174 173 147 126 124 102 83 46 37 36 18 12 7 6 EcoNI CCTnn'n_nnAGG Cuts at: 2093 3728 5444 2093 Size: 1635 1716 7093 Fragments arranged by size: 7093 1716 1635 EcoO109I rG'GnC_Cy Cuts at: 2098 3091 3621 3886 4492 4545 5066 5067 5596 Size: 993 530 265 606 53 521 1 529 Cuts at: 5596 6181 6862 2098 Size: 585 681 5680 Fragments arranged by size: 5680 993 681 606 585 530 529 521 265 53 1 EcoRI G'AATT_C Cuts at: 7753 8131 7753 Size: 378 10066 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6883 7406 6883 Size: 523 9921 FseI GG_CCGG'CC Cuts at: 2566 2566 Size: 10444 FspI TGC'GCA Cuts at: 8950 8950 Size: 10444 HaeII r_GCGC'y Cuts at: 1678 3792 4107 9591 1678 Size: 2114 315 5484 2531 Fragments arranged by size: 5484 2531 2114 315 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2502 2534 3392 3424 4850 Size: 32 135 32 930 32 858 32 1426 Cuts at: 4850 4882 4934 4966 5594 5626 5779 5811 6611 Size: 32 52 32 628 32 153 32 800 Cuts at: 6611 6643 7052 7084 7526 7558 9085 9117 9159 Size: 32 409 32 442 32 1527 32 42 Cuts at: 9159 9191 1373 Size: 32 2626 Fragments arranged by size: 2626 1527 1426 930 858 800 628 442 409 153 135 52 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2077 2367 2674 5974 6787 7657 Size: 988 281 139 290 307 3300 813 870 Cuts at: 7657 7788 8034 669 Size: 131 246 3079 Fragments arranged by size: 3300 3079 988 870 813 307 290 281 246 139 131 HindIII A'AGCT_T Cuts at: 748 7383 748 Size: 6635 3809 HpaI GTT'AAC Cuts at: 8034 8034 Size: 10444 KpnI G_GTAC'C Cuts at: 7769 7769 Size: 10444 MfeI C'AATT_G Cuts at: 8043 8043 Size: 10444 MluI A'CGCG_T Cuts at: 10320 10320 Size: 10444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3498 7095 9558 9742 1305 Size: 2193 3597 2463 184 2007 Fragments arranged by size: 3597 2463 2193 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4302 4564 4877 5535 6830 Size: 54 1425 343 2470 262 313 658 1295 Cuts at: 6830 7016 10 Size: 186 3438 Fragments arranged by size: 3438 2470 1425 1295 658 343 313 262 186 54 MslI CAynn'nnrTG Cuts at: 518 1166 3362 5858 5984 6608 6932 8402 8761 Size: 648 2196 2496 126 624 324 1470 359 Cuts at: 8761 8920 518 Size: 159 2042 Fragments arranged by size: 2496 2196 2042 1470 648 624 359 324 159 126 MspA1I CmG'CkG Cuts at: 1606 2229 2741 3462 4485 4687 4795 6008 7860 Size: 623 512 721 1023 202 108 1213 1852 Cuts at: 7860 8537 9685 9930 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1852 1213 1148 1023 721 677 623 512 245 202 108 NaeI GCC'GGC Cuts at: 2564 3694 4372 2564 Size: 1130 678 8636 Fragments arranged by size: 8636 1130 678 NcoI C'CATG_G Cuts at: 513 1065 2004 2665 3216 3736 5151 7154 513 Size: 552 939 661 551 520 1415 2003 3803 Fragments arranged by size: 3803 2003 1415 939 661 552 551 520 NdeI CA'TA_TG Cuts at: 387 6073 387 Size: 5686 4758 NgoMIV G'CCGG_C Cuts at: 2562 3692 4370 2562 Size: 1130 678 8636 Fragments arranged by size: 8636 1130 678 NheI G'CTAG_C Cuts at: 1052 5189 1052 Size: 4137 6307 NotI GC'GGCC_GC Cuts at: 7891 7891 Size: 10444 NruI TCG'CGA Cuts at: 7449 7449 Size: 10444 NspI r_CATG'y Cuts at: 1132 1229 2650 4370 6156 6703 6937 7802 9347 Size: 97 1421 1720 1786 547 234 865 1545 Cuts at: 9347 1132 Size: 2229 Fragments arranged by size: 2229 1786 1720 1545 1421 865 547 234 97 PasI CC'CwG_GG Cuts at: 1348 2925 4204 1348 Size: 1577 1279 7588 Fragments arranged by size: 7588 1577 1279 PciI A'CATG_T Cuts at: 6933 9343 6933 Size: 2410 8034 PflMI CCAn_nnn'nTGG Cuts at: 1108 2761 5394 6577 1108 Size: 1653 2633 1183 4975 Fragments arranged by size: 4975 2633 1653 1183 PfoI T'CCnGG_A Cuts at: 3317 3348 3714 4050 4062 5612 5838 6177 6615 Size: 31 366 336 12 1550 226 339 438 Cuts at: 6615 3317 Size: 7146 Fragments arranged by size: 7146 1550 438 366 339 336 226 31 12 PmeI GTTT'AAAC Cuts at: 7748 7748 Size: 10444 PmlI CAC'GTG Cuts at: 2700 2700 Size: 10444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4316 4348 8513 8545 10026 10058 4316 Size: 32 4165 32 1481 32 4702 Fragments arranged by size: 4702 4165 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3091 3621 4492 4545 6181 6862 3091 Size: 530 871 53 1636 681 6673 Fragments arranged by size: 6673 1636 871 681 530 53 PshAI GACnn'nnGTC Cuts at: 1613 3011 6822 1613 Size: 1398 3811 5235 Fragments arranged by size: 5235 3811 1398 PsiI TTA'TAA Cuts at: 8014 8014 Size: 10444 PspOMI G'GGCC_C Cuts at: 5066 5066 Size: 10444 PstI C_TGCA'G Cuts at: 830 2610 2878 3048 3606 3788 3885 4794 7796 Size: 1780 268 170 558 182 97 909 3002 Cuts at: 7796 830 Size: 3478 Fragments arranged by size: 3478 3002 1780 909 558 268 182 170 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10444 PvuII CAG'CTG Cuts at: 2741 3462 4485 4687 6008 2741 Size: 721 1023 202 1321 7177 Fragments arranged by size: 7177 1321 1023 721 202 SacI G_AGCT'C Cuts at: 3708 5630 6041 7692 3708 Size: 1922 411 1651 6460 Fragments arranged by size: 6460 1922 1651 411 SalI G'TCGA_C Cuts at: 1936 7786 1936 Size: 5850 4594 SanDI GG'GwC_CC Cuts at: 3621 4545 3621 Size: 924 9520 SapI GCTCTTCn'nnn_ Cuts at: 6285 6285 Size: 10444 SbfI CC_TGCA'GG Cuts at: 3048 3885 7796 3048 Size: 837 3911 5696 Fragments arranged by size: 5696 3911 837 ScaI AGT'ACT Cuts at: 8692 10404 8692 Size: 1712 8732 SfcI C'TryA_G Cuts at: 826 1045 2606 2874 3044 3496 3602 3784 3881 Size: 219 1561 268 170 452 106 182 97 Cuts at: 3881 4436 4790 6100 7792 8927 9608 9799 826 Size: 555 354 1310 1692 1135 681 191 1471 Fragments arranged by size: 1692 1561 1471 1310 1135 681 555 452 354 268 219 191 182 170 106 97 SfiI GGCCn_nnn'nGGCC Cuts at: 4561 4561 Size: 10444 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10444 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10444 SmaI CCC'GGG Cuts at: 1872 7771 1872 Size: 5899 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2068 2588 4391 5204 5417 6761 Size: 197 926 125 520 1803 813 213 1344 Cuts at: 6761 7391 8548 9449 9711 820 Size: 630 1157 901 262 1553 Fragments arranged by size: 1803 1553 1344 1157 926 901 813 630 520 262 213 197 125 SnaBI TAC'GTA Cuts at: 493 8235 493 Size: 7742 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10444 SphI G_CATG'C Cuts at: 1132 2650 4370 7802 1132 Size: 1518 1720 3432 3774 Fragments arranged by size: 3774 3432 1720 1518 SspI AAT'ATT Cuts at: 5 52 8368 5 Size: 47 8316 2081 Fragments arranged by size: 8316 2081 47 StuI AGG'CCT Cuts at: 1517 2729 3084 4094 4555 1517 Size: 1212 355 1010 461 7406 Fragments arranged by size: 7406 1212 1010 461 355 StyI C'CwwG_G Cuts at: 513 1065 2004 2665 2755 2954 3216 3481 3736 Size: 552 939 661 90 199 262 265 255 Cuts at: 3736 3768 5019 5151 5592 7011 7080 7154 7200 Size: 32 1251 132 441 1419 69 74 46 Cuts at: 7200 7883 513 Size: 683 3074 Fragments arranged by size: 3074 1419 1251 939 683 661 552 441 265 262 255 199 132 90 74 69 46 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3886 4136 4175 4949 8471 8488 8641 Size: 1255 2306 250 39 774 3522 17 153 Cuts at: 8641 8826 9271 325 Size: 185 445 1498 Fragments arranged by size: 3522 2306 1498 1255 774 445 250 185 153 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2694 4721 6091 Size: 274 80 33 51 1443 717 2027 1370 Cuts at: 6091 6696 6726 7218 7638 8690 10402 96 Size: 605 30 492 420 1052 1712 138 Fragments arranged by size: 2027 1712 1443 1370 1052 717 605 492 420 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3861 3893 5751 5783 6671 6703 10404 10436 3861 Size: 32 1858 32 888 32 3701 32 3869 Fragments arranged by size: 3869 3701 1858 888 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4816 6052 1316 Size: 3500 1236 5708 Fragments arranged by size: 5708 3500 1236 XbaI T'CTAG_A Cuts at: 7780 7780 Size: 10444 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2758 6607 2758 Size: 3849 6595 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10444 XmaI C'CCGG_G Cuts at: 1870 7769 1870 Size: 5899 4545 XmnI GAAnn'nnTTC Cuts at: 6485 8573 6485 Size: 2088 8356 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8252 276 Size: 53 83 186 936 6718 2468 Fragments arranged by size: 6718 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI EcoRV FspAI KasI NarI NsiI PacI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI