Restriction map for
pFN21AB1487
(Linear) (Six-base) MAPSORT of: KIBB1487.seq Check: 403 from: 1 to: 5739
KIBB1487.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1687 1753 1761 2089 5647 5680 5739
Size: 1687 66 8 328 3558 33 59
Fragments arranged by size:
3558 1687 328 66 59 33 8
AccI GT'mk_AC
Cuts at: 0 310 5739
Size: 310 5429
AclI AA'CG_TT
Cuts at: 0 5015 5739
Size: 5015 724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 997 1349 1579 1976 2296 2638 2764
Size: 422 575 352 230 397 320 342 126
Cuts at: 2764 3041 3124 3160 4304 4873 4910 5119 5125
Size: 277 83 36 1144 569 37 209 6
Cuts at: 5125 5384 5739
Size: 259 355
Fragments arranged by size:
1144 575 569 422 397 355 352 342
320 277 259 230 209 126 83 37
36 6
AfeI AGC'GCT
Cuts at: 0 2100 5739
Size: 2100 3639
AflIII A'CryG_T
Cuts at: 0 692 2324 4223 4928 5739
Size: 692 1632 1899 705 811
Fragments arranged by size:
1899 1632 811 705 692
AleI CACnn'nnGTG
Cuts at: 0 1357 3979 5739
Size: 1357 2622 1760
Fragments arranged by size:
2622 1760 1357
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 626 3358 5739
Size: 626 2732 2381
Fragments arranged by size:
2732 2381 626
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 731 763 3163 3195 5739
Size: 731 32 2400 32 2544
Fragments arranged by size:
2544 2400 731 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 651 1787 3309 4179 5739
Size: 651 1136 1522 870 1560
Fragments arranged by size:
1560 1522 1136 870 651
ApaI G_GGCC'C
Cuts at: 0 3065 5739
Size: 3065 2674
ApaLI G'TGCA_C
Cuts at: 0 2254 2833 4744 5739
Size: 2254 579 1911 995
Fragments arranged by size:
2254 1911 995 579
ApoI r'AATT_y
Cuts at: 0 1243 4420 4876 5739
Size: 1243 3177 456 863
Fragments arranged by size:
3177 1243 863 456
AvaI C'yCGr_G
Cuts at: 0 4333 5509 5739
Size: 4333 1176 230
Fragments arranged by size:
4333 1176 230
BamHI G'GATC_C
Cuts at: 0 57 5739
Size: 57 5682
BanI G'GyrC_C
Cuts at: 0 1351 1735 2152 2910 4792 4867 5739
Size: 1351 384 417 758 1882 75 872
Fragments arranged by size:
1882 1351 872 758 417 384 75
BanII G_rGCy'C
Cuts at: 0 185 1703 2454 3065 3625 4036 4334 4910
Size: 185 1518 751 611 560 411 298 576
Cuts at: 4910 5300 5687 5739
Size: 390 387 52
Fragments arranged by size:
1518 751 611 576 560 411 390 387
298 185 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 1024 2597 2741 3356 5274 5739
Size: 1024 1573 144 615 1918 465
Fragments arranged by size:
1918 1573 1024 615 465 144
BbvCI CC'TCA_GC
Cuts at: 0 941 5022 5399 5739
Size: 941 4081 377 340
Fragments arranged by size:
4081 941 377 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 524 558 5739
Size: 524 34 5181
Fragments arranged by size:
5181 524 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2053 5491 5739
Size: 2053 3438 248
Fragments arranged by size:
3438 2053 248
BclI T'GATC_A
Cuts at: 0 4895 5739
Size: 4895 844
BglI GCCn_nnn'nGGC
Cuts at: 0 1693 2556 3060 5739
Size: 1693 863 504 2679
Fragments arranged by size:
2679 1693 863 504
BglII A'GATC_T
Cuts at: 0 4106 4703 5060 5739
Size: 4106 597 357 679
Fragments arranged by size:
4106 679 597 357
BlpI GC'TnA_GC
Cuts at: 0 1697 5739
Size: 1697 4042
Bme1580I G_kGCm'C
Cuts at: 0 478 615 1628 2074 2258 2837 3065 4748
Size: 478 137 1013 446 184 579 228 1683
Cuts at: 4748 4795 5739
Size: 47 944
Fragments arranged by size:
1683 1013 944 579 478 446 228 184
137 47
BmgBI CAC'GTC
Cuts at: 0 681 2325 2604 4130 5739
Size: 681 1644 279 1526 1609
Fragments arranged by size:
1644 1609 1526 681 279
BmrI ACTGGGnnnn_n'
Cuts at: 0 1615 5739
Size: 1615 4124
BmtI G_CTAG'C
Cuts at: 0 3188 5739
Size: 3188 2551
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1139 2042 5739
Size: 1139 903 3697
Fragments arranged by size:
3697 1139 903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 876 883 985 1121 1533 2123 3334 3554
Size: 876 7 102 136 412 590 1211 220
Cuts at: 3554 4633 4855 5739
Size: 1079 222 884
Fragments arranged by size:
1211 1079 884 876 590 412 222 220
136 102 7
Bpu10I CC'TnA_GC
Cuts at: 0 941 3040 4556 5022 5399 5739
Size: 941 2099 1516 466 377 340
Fragments arranged by size:
2099 1516 941 466 377 340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 568 2371 3184 3397 4741 5407 5739
Size: 48 520 1803 813 213 1344 666 332
Fragments arranged by size:
1803 1344 813 666 520 332 213 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 442 2687 2995 3091 3127 4853 5739
Size: 442 2245 308 96 36 1726 886
Fragments arranged by size:
2245 1726 886 442 308 96 36
BsaAI yAC'GTr
Cuts at: 0 695 2075 4213 5739
Size: 695 1380 2138 1526
Fragments arranged by size:
2138 1526 1380 695
BsaBI GATnn'nnATC
Cuts at: 0 4410 4531 5739
Size: 4410 121 1208
Fragments arranged by size:
4410 1208 121
BsaWI w'CCGG_w
Cuts at: 0 1005 4346 5201 5739
Size: 1005 3341 855 538
Fragments arranged by size:
3341 1005 855 538
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1657 1687 3746 3776 4606 4636 4666 4696
Size: 1657 30 2059 30 830 30 30 30
Cuts at: 4696 5739
Size: 1043
Fragments arranged by size:
2059 1657 1043 830 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 1115 1377 2516 2599 2665 4026 4053
Size: 307 808 262 1139 83 66 1361 27
Cuts at: 4053 4414 5739
Size: 361 1325
Fragments arranged by size:
1361 1325 1139 808 361 307 262 83
66 27
BseYI C'CCAG_C
Cuts at: 0 498 1849 2156 2754 2843 2952 3291 3375
Size: 498 1351 307 598 89 109 339 84
Cuts at: 3375 3945 3999 5739
Size: 570 54 1740
Fragments arranged by size:
1740 1351 598 570 498 339 307 109
89 84 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1443 1847 1954 2371 5112 5658 5739
Size: 1443 404 107 417 2741 546 81
Fragments arranged by size:
2741 1443 546 417 404 107 81
BsiHKAI G_wGCw'C
Cuts at: 0 342 1074 1606 1703 2129 2258 2837 3544
Size: 342 732 532 97 426 129 579 707
Cuts at: 3544 3625 3848 3911 4036 4748 5687 5739
Size: 81 223 63 125 712 939 52
Fragments arranged by size:
939 732 712 707 579 532 426 342
223 129 125 97 81 63 52
BsiWI C'GTAC_G
Cuts at: 0 1608 5739
Size: 1608 4131
BsmI GAATG_Cn'
Cuts at: 0 1234 1764 2410 3015 4845 5050 5739
Size: 1234 530 646 605 1830 205 689
Fragments arranged by size:
1830 1234 689 646 605 530 205
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2626 3468 5739
Size: 2626 842 2271
Fragments arranged by size:
2626 2271 842
Bsp1286I G_dGCh'C
Cuts at: 0 185 342 478 615 1074 1606 1628 1703
Size: 185 157 136 137 459 532 22 75
Cuts at: 1703 2074 2129 2258 2454 2837 3065 3544 3625
Size: 371 55 129 196 383 228 479 81
Cuts at: 3625 3848 3911 4036 4334 4748 4795 4910 5300
Size: 223 63 125 298 414 47 115 390
Cuts at: 5300 5687 5739
Size: 387 52
Fragments arranged by size:
532 479 459 414 390 387 383 371
298 228 223 196 185 157 137 136
129 125 115 81 75 63 55 52
47 22
BspHI T'CATG_A
Cuts at: 0 4535 5739
Size: 4535 1204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 561 636 1687 1753 1761 2089 2792 2838
Size: 561 75 1051 66 8 328 703 46
Cuts at: 2838 2934 3684 4187 5647 5680 5739
Size: 96 750 503 1460 33 59
Fragments arranged by size:
1460 1051 750 703 561 503 328 96
75 66 59 46 33 8
BsrBI CCG'CTC
Cuts at: 0 5340 5739
Size: 5340 399
BsrDI GCAATG_nn'
Cuts at: 0 3051 3159 3710 5253 5291 5739
Size: 3051 108 551 1543 38 448
Fragments arranged by size:
3051 1543 551 448 108 38
BsrFI r'CCGG_y
Cuts at: 0 557 1687 2365 5739
Size: 557 1130 678 3374
Fragments arranged by size:
3374 1130 678 557
BsrGI T'GTAC_A
Cuts at: 0 689 4086 5739
Size: 689 3397 1653
Fragments arranged by size:
3397 1653 689
BssHII G'CGCG_C
Cuts at: 0 3898 5739
Size: 3898 1841
BstAPI GCAn_nnn'nTGC
Cuts at: 0 887 3309 5135 5739
Size: 887 2422 1826 604
Fragments arranged by size:
2422 1826 887 604
BstBI TT'CG_AA
Cuts at: 0 155 5739
Size: 155 5584
BstEII G'GTnAC_C
Cuts at: 0 172 573 822 1791 2236 5324 5739
Size: 172 401 249 969 445 3088 415
Fragments arranged by size:
3088 969 445 415 401 249 172
BstXI CCAn_nnnn'nTGG
Cuts at: 0 907 3021 4833 5175 5739
Size: 907 2114 1812 342 564
Fragments arranged by size:
2114 1812 907 564 342
BstYI r'GATC_y
Cuts at: 0 57 1985 2378 2750 3830 4106 4411 4703
Size: 57 1928 393 372 1080 276 305 292
Cuts at: 4703 5060 5739
Size: 357 679
Fragments arranged by size:
1928 1080 679 393 372 357 305 292
276 57
BstZ17I GTA'TAC
Cuts at: 0 311 5739
Size: 311 5428
Bsu36I CC'TnA_GG
Cuts at: 0 417 2545 3485 3731 5739
Size: 417 2128 940 246 2008
Fragments arranged by size:
2128 2008 940 417 246
BtgI C'CryG_G
Cuts at: 0 660 1211 1731 2458 3074 3146 3557 5149
Size: 660 551 520 727 616 72 411 1592
Cuts at: 5149 5739
Size: 590
Fragments arranged by size:
1592 727 660 616 590 551 520 411
72
BtsI GCAGTG_nn'
Cuts at: 0 339 449 598 1460 1606 1859 3000 3026
Size: 339 110 149 862 146 253 1141 26
Cuts at: 3026 3478 3909 3925 4086 5140 5739
Size: 452 431 16 161 1054 599
Fragments arranged by size:
1141 1054 862 599 452 431 339 253
161 149 146 110 26 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 315 350 5739
Size: 315 35 5389
Fragments arranged by size:
5389 315 35
DraIII CAC_nnn'GTG
Cuts at: 0 4015 5135 5739
Size: 4015 1120 604
Fragments arranged by size:
4015 1120 604
EaeI y'GGCC_r
Cuts at: 0 555 2295 2367 2557 2870 3528 3893 4823
Size: 555 1740 72 190 313 658 365 930
Cuts at: 4823 5009 5739
Size: 186 730
Fragments arranged by size:
1740 930 730 658 555 365 313 190
186 72
EarI CTCTTCn'nnn_
Cuts at: 0 1100 1108 1804 1816 2614 2816 3632 4280
Size: 1100 8 696 12 798 202 816 648
Cuts at: 4280 5720 5729 5739
Size: 1440 9 10
Fragments arranged by size:
1440 1100 816 798 696 648 202 12
10 9 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2650 3550 5739
Size: 2650 900 2189
Fragments arranged by size:
2650 2189 900
EcoICRI GAG'CTC
Cuts at: 0 1701 3623 4034 5685 5739
Size: 1701 1922 411 1651 54
Fragments arranged by size:
1922 1701 1651 411 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 876 883 985 997 1121 1349 1533
Size: 422 454 7 102 12 124 228 184
Cuts at: 1533 1579 1976 2123 2296 2638 2764 3041 3124
Size: 46 397 147 173 342 126 277 83
Cuts at: 3124 3160 3334 3554 4304 4633 4855 4873 4910
Size: 36 174 220 750 329 222 18 37
Cuts at: 4910 5119 5125 5384 5739
Size: 209 6 259 355
Fragments arranged by size:
750 454 422 397 355 342 329 277
259 228 222 220 209 184 174 173
147 126 124 102 83 46 37 36
18 12 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 88 1723 3439 5739
Size: 88 1635 1716 2300
Fragments arranged by size:
2300 1716 1635 88
EcoO109I rG'GnC_Cy
Cuts at: 0 93 1086 1616 1881 2487 2540 3061 3062
Size: 93 993 530 265 606 53 521 1
Cuts at: 3062 3591 4176 4857 5739
Size: 529 585 681 882
Fragments arranged by size:
993 882 681 606 585 530 529 521
265 93 53 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4878 5401 5739
Size: 4878 523 338
Fragments arranged by size:
4878 523 338
FseI GG_CCGG'CC
Cuts at: 0 561 5739
Size: 561 5178
HaeII r_GCGC'y
Cuts at: 0 1787 2102 5739
Size: 1787 315 3637
Fragments arranged by size:
3637 1787 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 497 529 1387 1419 2845 2877 2929 2961
Size: 497 32 858 32 1426 32 52 32
Cuts at: 2961 3589 3621 3774 3806 4606 4638 5047 5079
Size: 628 32 153 32 800 32 409 32
Cuts at: 5079 5521 5553 5739
Size: 442 32 186
Fragments arranged by size:
1426 858 800 628 497 442 409 186
153 52 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 72 362 669 3969 4782 5652 5739
Size: 72 290 307 3300 813 870 87
Fragments arranged by size:
3300 870 813 307 290 87 72
HindIII A'AGCT_T
Cuts at: 0 5378 5739
Size: 5378 361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1493 5090 5739
Size: 1493 3597 649
Fragments arranged by size:
3597 1493 649
MscI TGG'CCA
Cuts at: 0 2297 2559 2872 3530 4825 5011 5739
Size: 2297 262 313 658 1295 186 728
Fragments arranged by size:
2297 1295 728 658 313 262 186
MslI CAynn'nnrTG
Cuts at: 0 1357 3853 3979 4603 4927 5739
Size: 1357 2496 126 624 324 812
Fragments arranged by size:
2496 1357 812 624 324 126
MspA1I CmG'CkG
Cuts at: 0 224 736 1457 2480 2682 2790 4003 5739
Size: 224 512 721 1023 202 108 1213 1736
Fragments arranged by size:
1736 1213 1023 721 512 224 202 108
NaeI GCC'GGC
Cuts at: 0 559 1689 2367 5739
Size: 559 1130 678 3372
Fragments arranged by size:
3372 1130 678 559
NcoI C'CATG_G
Cuts at: 0 660 1211 1731 3146 5149 5739
Size: 660 551 520 1415 2003 590
Fragments arranged by size:
2003 1415 660 590 551 520
NdeI CA'TA_TG
Cuts at: 0 4068 5739
Size: 4068 1671
NgoMIV G'CCGG_C
Cuts at: 0 557 1687 2365 5739
Size: 557 1130 678 3374
Fragments arranged by size:
3374 1130 678 557
NheI G'CTAG_C
Cuts at: 0 3184 5739
Size: 3184 2555
NruI TCG'CGA
Cuts at: 0 5444 5739
Size: 5444 295
NspI r_CATG'y
Cuts at: 0 645 2365 4151 4698 4932 5739
Size: 645 1720 1786 547 234 807
Fragments arranged by size:
1786 1720 807 645 547 234
PasI CC'CwG_GG
Cuts at: 0 920 2199 5739
Size: 920 1279 3540
Fragments arranged by size:
3540 1279 920
PciI A'CATG_T
Cuts at: 0 4928 5739
Size: 4928 811
PflMI CCAn_nnn'nTGG
Cuts at: 0 756 3389 4572 5739
Size: 756 2633 1183 1167
Fragments arranged by size:
2633 1183 1167 756
PfoI T'CCnGG_A
Cuts at: 0 1312 1343 1709 2045 2057 3607 3833 4172
Size: 1312 31 366 336 12 1550 226 339
Cuts at: 4172 4610 5739
Size: 438 1129
Fragments arranged by size:
1550 1312 1129 438 366 339 336 226
31 12
PmlI CAC'GTG
Cuts at: 0 695 5739
Size: 695 5044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2311 2343 5739
Size: 2311 32 3396
Fragments arranged by size:
3396 2311 32
PpuMI rG'GwC_Cy
Cuts at: 0 1086 1616 2487 2540 4176 4857 5739
Size: 1086 530 871 53 1636 681 882
Fragments arranged by size:
1636 1086 882 871 681 530 53
PshAI GACnn'nnGTC
Cuts at: 0 1006 4817 5739
Size: 1006 3811 922
Fragments arranged by size:
3811 1006 922
PspOMI G'GGCC_C
Cuts at: 0 3061 5739
Size: 3061 2678
PstI C_TGCA'G
Cuts at: 0 605 873 1043 1601 1783 1880 2789 5739
Size: 605 268 170 558 182 97 909 2950
Fragments arranged by size:
2950 909 605 558 268 182 170 97
PvuII CAG'CTG
Cuts at: 0 736 1457 2480 2682 4003 5739
Size: 736 721 1023 202 1321 1736
Fragments arranged by size:
1736 1321 1023 736 721 202
SacI G_AGCT'C
Cuts at: 0 1703 3625 4036 5687 5739
Size: 1703 1922 411 1651 52
Fragments arranged by size:
1922 1703 1651 411 52
SanDI GG'GwC_CC
Cuts at: 0 1616 2540 5739
Size: 1616 924 3199
Fragments arranged by size:
3199 1616 924
SapI GCTCTTCn'nnn_
Cuts at: 0 4280 5739
Size: 4280 1459
SbfI CC_TGCA'GG
Cuts at: 0 1043 1880 5739
Size: 1043 837 3859
Fragments arranged by size:
3859 1043 837
SfcI C'TryA_G
Cuts at: 0 601 869 1039 1491 1597 1779 1876 2431
Size: 601 268 170 452 106 182 97 555
Cuts at: 2431 2785 4095 5739
Size: 354 1310 1644
Fragments arranged by size:
1644 1310 601 555 452 354 268 182
170 106 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2556 5739
Size: 2556 3183
SmlI C'TyrA_G
Cuts at: 0 63 583 2386 3199 3412 4756 5386 5739
Size: 63 520 1803 813 213 1344 630 353
Fragments arranged by size:
1803 1344 813 630 520 353 213 63
SphI G_CATG'C
Cuts at: 0 645 2365 5739
Size: 645 1720 3374
Fragments arranged by size:
3374 1720 645
StuI AGG'CCT
Cuts at: 0 724 1079 2089 2550 5739
Size: 724 355 1010 461 3189
Fragments arranged by size:
3189 1010 724 461 355
StyI C'CwwG_G
Cuts at: 0 660 750 949 1211 1476 1731 1763 3014
Size: 660 90 199 262 265 255 32 1251
Cuts at: 3014 3146 3587 5006 5075 5149 5195 5739
Size: 132 441 1419 69 74 46 544
Fragments arranged by size:
1419 1251 660 544 441 265 262 255
199 132 90 74 69 46 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1881 2131 2170 2944 5739
Size: 1881 250 39 774 2795
Fragments arranged by size:
2795 1881 774 250 39
TatI w'GTAC_w
Cuts at: 0 689 2716 4086 4691 4721 5213 5633 5739
Size: 689 2027 1370 605 30 492 420 106
Fragments arranged by size:
2027 1370 689 605 492 420 106 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1856 1888 3746 3778 4666 4698 5739
Size: 1856 32 1858 32 888 32 1041
Fragments arranged by size:
1858 1856 1041 888 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2811 4047 5739
Size: 2811 1236 1692
Fragments arranged by size:
2811 1692 1236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 753 4602 5739
Size: 753 3849 1137
Fragments arranged by size:
3849 1137 753
XmnI GAAnn'nnTTC
Cuts at: 0 4480 5739
Size: 4480 1259
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NruI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuII SacI SanDI SapI SbfI SfcI
SfiI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AgeI AhdI AscI AseI AsiSI
AvrII BaeI BbeI BfrBI BsaHI BsiEI BspEI BssSI
BtgZI ClaI DraI DrdI EagI EcoRI EcoRV FspI
FspAI HpaI KasI KpnI MfeI MluI NarI NotI
NsiI PacI PmeI PsiI PspXI PsrI PvuI RsrII
SacII SalI ScaI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI
ZraI