Restriction map for
pFN21AB1440
##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI Asp700I AspEI AvrII
BcgI BfrI BglII BlnI BsaBI
BsmBI BssHII Bst1107I BstBI BstZ17I
DraI Eam1105I EcoRV EcoT22I HpaI
MamI MfeI MluI MunI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I SbfI
SfiI SfuI SgrAI SnaBI Sse8387I
SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 3381 GGTACC G^GTACC
AcsI 4077 GAATTC [GA]^AATT[CT]
ApoI 4077 GAATTC [GA]^AATT[CT]
Asp718I 3381 GGTACC G^GTACC
AspI 2754 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 1336 GGATAC GTATCC(6/5)
BsiWI 870 CGTACG C^GTACG
BsmI 4224 GAATGC GAATGC(1/-1)
BspHI 4313 TCATGA T^CATGA
BspLU11I 5570 ACATGT A^CATGT
CpoI 1865 CGGTCCG CG^G[AT]CCG
EcoRI 4077 GAATTC G^AATTC
FseI 2437 GGCCGGCC GGCCGG^CC
HindIII 5191 AAGCTT A^AGCTT
KpnI 3381 GGTACC GGTAC^C
NaeI 2438 GCCGGC GCC^GGC
NdeI 3527 CATATG CA^TATG
NgoMIV 2438 GCCGGC G^CCGGC
NheI 2298 GCTAGC G^CTAGC
PflFI 2754 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3869 CGATCG CGAT^CG
RcaI 4313 TCATGA T^CATGA
RsrII 1865 CGGTCCG CG^G[AT]CCG
SalI 5566 GTCGAC G^TCGAC
SapI 949 GCTCTTC GCTCTTC(1/4)
SpeI 352 ACTAGT A^CTAGT
Tth111I 2754 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3459 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2290 GACGTC GACGT^C
3048 GACGTC
AccIII 1356 TCCGGA T^CCGGA
3618 TCCGGA
AocI 115 CCTGAGG CC^T[ACGT]AGG
1606 CCTCAGG
AviII 2102 TGCGCA TGC^GCA
4442 TGCGCA
BamHI 275 GGATCC G^GATCC
1580 GGATCC
BbeI 76 GGCGCC GGCGC^C
1233 GGCGCC
BbvCI 421 CCTCAGC CCTCAGC(-5/-2)
1134 CCTCAGC
BclI 2453 TGATCA T^GATCA
4353 TGATCA
BsaWI 1356 TCCGGA [AT]^CCGG[AT]
3618 TCCGGA
BseAI 1356 TCCGGA T^CCGGA
3618 TCCGGA
Bsp1407I 319 TGTACA T^GTACA
3113 TGTACA
BspDI 4162 ATCGAT AT^CGAT
4204 ATCGAT
BspEI 1356 TCCGGA T^CCGGA
3618 TCCGGA
BsrBI 1612 GAGCGG CCGCTC(-3/-3)
4669 GAGCGG
BsrGI 319 TGTACA T^GTACA
3113 TGTACA
Bsu36I 115 CCTGAGG CC^T[ACGT]AGG
1606 CCTCAGG
ClaI 4162 ATCGAT AT^CGAT
4204 ATCGAT
DraIII 1669 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3238 CACCTGGTG
DrdI 126 GACTGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2926 GACATCAAGGTC
EagI 543 CGGCCG C^GGCCG
3312 CGGCCG
EarI 950 CTCTTC CTCTTC(1/4)
376 GAAGAG
EclXI 543 CGGCCG C^GGCCG
3312 CGGCCG
Eco52I 543 CGGCCG C^GGCCG
3312 CGGCCG
Eco81I 115 CCTGAGG CC^T[ACGT]AGG
1606 CCTCAGG
EcoNI 2616 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4392 CCTTATTCAGG
FbaI 2453 TGATCA T^GATCA
4353 TGATCA
FspI 2102 TGCGCA TGC^GCA
4442 TGCGCA
HincII 5269 GTTGAC GT[CT]^[GA]AC
5566 GTCGAC
HindII 5269 GTTGAC GT[CT]^[GA]AC
5566 GTCGAC
KasI 76 GGCGCC G^GCGCC
1233 GGCGCC
Ksp632I 950 CTCTTC CTCTTC(1/4)
376 GAAGAG
MroI 1356 TCCGGA T^CCGGA
3618 TCCGGA
MseI 107 TTAA T^TAA
3104 TTAA
NarI 76 GGCGCC GG^CGCC
1233 GGCGCC
PaeR7I 4142 CTCGAG C^TCGAG
4894 CTCGAG
SfoI 76 GGCGCC GGC^GCC
1233 GGCGCC
SspBI 319 TGTACA T^GTACA
3113 TGTACA
StuI 4574 AGGCCT AGG^CCT
5093 AGGCCT
Tru9I 107 TTAA T^TAA
3104 TTAA
XhoI 4142 CTCGAG C^TCGAG
4894 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco47III EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScaI ScrFI SexAI SfaNI
SfcI SmaI SmlI SphI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI