(Circular) (Six-base) MAPSORT of: pFN21AB1440.seq Check: 5381 from: 1 to: 10399 pFN21AB1440 10399 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7429 7429 Size: 10399 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4299 5057 8209 278 Size: 53 83 186 936 2763 758 3152 2468 Fragments arranged by size: 3152 2763 2468 936 758 186 83 53 AccI GT'mk_AC Cuts at: 1937 2529 3351 4185 4766 7572 7742 1937 Size: 592 822 834 581 2806 170 4594 Fragments arranged by size: 4594 2806 834 822 592 581 170 Acc65I G'GTAC_C Cuts at: 5386 7720 5386 Size: 2334 8065 AclI AA'CG_TT Cuts at: 1563 8526 8899 1563 Size: 6963 373 3063 Fragments arranged by size: 6963 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 3740 5546 5903 8462 9846 10114 1632 Size: 821 1287 1806 357 2559 1384 268 1917 Fragments arranged by size: 2559 1917 1806 1384 1287 821 357 268 AfeI AGC'GCT Cuts at: 2234 5684 7247 2234 Size: 3450 1563 5386 Fragments arranged by size: 5386 3450 1563 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10202 AflIII A'CryG_T Cuts at: 1203 2648 6768 6933 7575 9298 10275 1203 Size: 1445 4120 165 642 1723 977 1327 Fragments arranged by size: 4120 1723 1445 1327 977 642 165 AgeI A'CCGG_T Cuts at: 9230 9230 Size: 10399 AhdI GACnn_n'nnGTC Cuts at: 9128 9128 Size: 10399 AleI CACnn'nnGTG Cuts at: 1166 2897 3425 3482 4070 4175 4247 4835 6468 Size: 1731 528 57 588 105 72 588 1633 Cuts at: 6468 6818 7494 7517 1166 Size: 350 676 23 4048 Fragments arranged by size: 4048 1731 1633 676 588 588 528 350 105 72 57 23 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3455 4115 5034 7512 1184 Size: 2271 660 919 2478 4071 Fragments arranged by size: 4071 2478 2271 919 660 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6887 6919 6887 Size: 32 10367 AlwNI CAG_nnn'CTG Cuts at: 2495 2663 4867 5245 6856 8142 9714 2495 Size: 168 2204 378 1611 1286 1572 3180 Fragments arranged by size: 3180 2204 1611 1572 1286 378 168 ApaI G_GGCC'C Cuts at: 3089 4079 6254 6606 3089 Size: 990 2175 352 6882 Fragments arranged by size: 6882 2175 990 352 ApaLI G'TGCA_C Cuts at: 2775 6614 7487 8456 9612 2775 Size: 3839 873 969 1156 3562 Fragments arranged by size: 3839 3562 1156 969 873 ApoI r'AATT_y Cuts at: 1493 6082 7708 7934 8086 10195 1493 Size: 4589 1626 226 152 2109 1697 Fragments arranged by size: 4589 2109 1697 1626 226 152 AseI AT'TA_AT Cuts at: 160 1032 8953 160 Size: 872 7921 1606 Fragments arranged by size: 7921 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10399 AvaI C'yCGr_G Cuts at: 1870 1943 3314 4085 4709 5438 6147 6179 6260 Size: 73 1371 771 624 729 709 32 81 Cuts at: 6260 6345 6899 7338 7724 1870 Size: 85 554 439 386 4545 Fragments arranged by size: 4545 1371 771 729 709 624 554 439 386 85 81 73 32 BamHI G'GATC_C Cuts at: 2280 3585 7729 8096 2280 Size: 1305 4144 367 4583 Fragments arranged by size: 4583 4144 1305 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2081 2573 3238 4394 Size: 325 217 627 251 43 492 665 1156 Cuts at: 4394 4497 5386 6380 6820 7720 9175 618 Size: 103 889 994 440 900 1455 1842 Fragments arranged by size: 1842 1455 1156 994 900 889 665 627 492 440 325 251 217 103 43 BanII G_rGCy'C Cuts at: 1391 2820 2865 3089 3324 3417 3863 4079 4272 Size: 1429 45 224 235 93 446 216 193 Cuts at: 4272 6254 6606 6900 7052 7718 1391 Size: 1982 352 294 152 666 4072 Fragments arranged by size: 4072 1982 1429 666 446 352 294 235 224 216 193 152 93 45 BbeI G_GCGC'C Cuts at: 2085 3242 2085 Size: 1157 9242 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 3669 3772 5057 6698 7598 928 Size: 971 199 1571 103 1285 1641 900 3729 Fragments arranged by size: 3729 1641 1571 1285 971 900 199 103 BbvCI CC'TCA_GC Cuts at: 2427 3140 2427 Size: 713 9686 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8590 8624 1903 Size: 34 6653 34 3678 Fragments arranged by size: 6653 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3335 8291 9501 3335 Size: 4956 1210 4233 Fragments arranged by size: 4956 4233 1210 BclI T'GATC_A Cuts at: 1548 4458 6358 1548 Size: 2910 1900 5589 Fragments arranged by size: 5589 2910 1900 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3912 3944 4132 Size: 107 122 71 631 605 2240 32 188 Cuts at: 4132 4562 6642 9010 136 Size: 430 2080 2368 1525 Fragments arranged by size: 2368 2240 2080 1525 631 605 430 188 122 107 71 32 BglII A'GATC_T Cuts at: 10394 10394 Size: 10399 BlpI GC'TnA_GC Cuts at: 2864 3278 5066 7816 2864 Size: 414 1788 2750 5447 Fragments arranged by size: 5447 2750 1788 414 Bme1580I G_kGCm'C Cuts at: 1790 2043 2779 3080 3089 3223 4063 4079 4128 Size: 253 736 301 9 134 840 16 49 Cuts at: 4128 4399 5106 5151 6254 6606 6618 7404 7491 Size: 271 707 45 1103 352 12 786 87 Cuts at: 7491 8460 9616 1790 Size: 969 1156 2573 Fragments arranged by size: 2573 1156 1103 969 840 786 736 707 352 301 271 253 134 87 49 45 16 12 9 BmgBI CAC'GTC Cuts at: 1318 4072 4123 4764 6936 1318 Size: 2754 51 641 2172 4781 Fragments arranged by size: 4781 2754 2172 641 51 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2142 3655 3805 Size: 416 53 83 399 24 725 1513 150 Cuts at: 3805 3919 7297 8130 9088 442 Size: 114 3378 833 958 1753 Fragments arranged by size: 3378 1753 1513 958 833 725 416 399 150 114 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4307 1056 Size: 3251 7148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2879 3229 3988 4046 4277 4475 5468 5677 Size: 1640 350 759 58 231 198 993 209 Cuts at: 5677 6113 6301 6353 6460 6812 6855 7565 7685 Size: 436 188 52 107 352 43 710 120 Cuts at: 7685 9059 1239 Size: 1374 2579 Fragments arranged by size: 2579 1640 1374 993 759 710 436 352 350 231 209 198 188 120 107 58 52 43 Bpu10I CC'TnA_GC Cuts at: 2013 2427 3140 2013 Size: 414 713 9272 Fragments arranged by size: 9272 713 414 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4895 5650 6011 8524 9389 9687 4895 Size: 755 361 2513 865 298 5607 Fragments arranged by size: 5607 2513 865 755 361 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 5952 6257 6380 9062 882 Size: 617 2841 1612 305 123 2682 2219 Fragments arranged by size: 2841 2682 2219 1612 617 305 123 BsaAI yAC'GTr Cuts at: 493 1204 2791 4249 6075 6715 6769 6804 8190 Size: 711 1587 1458 1826 640 54 35 1386 Cuts at: 8190 493 Size: 2702 Fragments arranged by size: 2702 1826 1587 1458 1386 711 640 54 35 BsaBI GATnn'nnATC Cuts at: 10393 10393 Size: 10399 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 3239 3777 4296 Size: 53 83 186 936 549 1157 538 519 Cuts at: 4296 5054 5420 6174 8206 8588 9223 275 Size: 758 366 754 2032 382 635 1451 Fragments arranged by size: 2032 1451 1157 936 758 754 635 549 538 519 382 366 186 83 53 BsaWI w'CCGG_w Cuts at: 3361 5623 8832 9230 9504 9651 10062 3361 Size: 2262 3209 398 274 147 411 3698 Fragments arranged by size: 3698 3209 2262 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3680 3710 5000 5030 5747 Size: 30 165 30 2277 30 1290 30 717 Cuts at: 5747 5777 5833 5863 6032 6062 6326 6356 6749 Size: 30 56 30 169 30 264 30 393 Cuts at: 6749 6779 6887 6917 1178 Size: 30 108 30 4660 Fragments arranged by size: 4660 2277 1290 717 393 264 169 165 108 56 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2688 2828 2899 3332 3407 3688 4697 5504 Size: 1502 140 71 433 75 281 1009 807 Cuts at: 5504 5762 5896 6350 1186 Size: 258 134 454 5235 Fragments arranged by size: 5235 1502 1009 807 454 433 281 258 140 134 75 71 BseYI C'CCAG_C Cuts at: 2229 2717 2914 3203 3500 3543 4008 4127 4205 Size: 488 197 289 297 43 465 119 78 Cuts at: 4205 4528 4721 4979 5070 5736 6856 9602 10098 Size: 323 193 258 91 666 1120 2746 496 Cuts at: 10098 2229 Size: 2530 Fragments arranged by size: 2746 2530 1120 666 496 488 465 323 297 289 258 197 193 119 91 78 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2037 2318 3284 3320 3738 4452 4607 Size: 573 301 281 966 36 418 714 155 Cuts at: 4607 6770 7643 1163 Size: 2163 873 3919 Fragments arranged by size: 3919 2163 966 873 714 573 418 301 281 155 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2551 3426 5320 5877 7849 8610 Size: 275 186 550 875 1894 557 1972 761 Cuts at: 8610 9638 1540 Size: 1028 2301 Fragments arranged by size: 2301 1972 1894 1028 875 761 557 550 275 186 BsiHKAI G_wGCw'C Cuts at: 2379 2779 2820 2865 4002 5435 5559 6618 6900 Size: 400 41 45 1137 1433 124 1059 282 Cuts at: 6900 7136 7167 7491 8460 8545 9616 2379 Size: 236 31 324 969 85 1071 3162 Fragments arranged by size: 3162 1433 1137 1071 1059 969 400 324 282 236 124 85 45 41 31 BsiWI C'GTAC_G Cuts at: 2875 2875 Size: 10399 BsmI GAATG_Cn' Cuts at: 6235 7910 8003 6235 Size: 1675 93 8631 Fragments arranged by size: 8631 1675 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2379 2779 2820 2865 3080 3089 Size: 399 253 336 400 41 45 215 9 Cuts at: 3089 3223 3324 3417 3863 4002 4063 4079 4128 Size: 134 101 93 446 139 61 16 49 Cuts at: 4128 4272 4399 5106 5151 5435 5559 6254 6606 Size: 144 127 707 45 284 124 695 352 Cuts at: 6606 6618 6900 7052 7136 7167 7404 7491 7718 Size: 12 282 152 84 31 237 87 227 Cuts at: 7718 8460 8545 9616 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 707 695 446 400 399 352 336 284 282 253 237 227 215 152 144 139 134 127 124 101 93 87 85 84 61 49 45 45 41 31 16 12 9 BspEI T'CCGG_A Cuts at: 3361 5623 10062 3361 Size: 2262 4439 3698 Fragments arranged by size: 4439 3698 2262 BspHI T'CATG_A Cuts at: 6318 8288 10018 6318 Size: 1970 1730 6699 Fragments arranged by size: 6699 1970 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4725 4749 5184 6728 6966 7429 7754 844 Size: 3881 24 435 1544 238 463 325 3489 Fragments arranged by size: 3881 3489 1544 463 435 325 238 24 BsrBI CCG'CTC Cuts at: 1394 1823 3619 6676 8286 1394 Size: 429 1796 3057 1610 3507 Fragments arranged by size: 3507 3057 1796 1610 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2288 2875 5052 8894 9068 65 Size: 1183 1040 587 2177 3842 174 1396 Fragments arranged by size: 3842 2177 1396 1183 1040 587 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4443 4536 4616 5002 5610 6641 6981 Size: 110 2633 93 80 386 608 1031 340 Cuts at: 6981 9043 9230 1700 Size: 2062 187 2869 Fragments arranged by size: 2869 2633 2062 1031 608 386 340 187 110 93 80 BsrGI T'GTAC_A Cuts at: 96 2324 5118 96 Size: 2228 2794 5377 Fragments arranged by size: 5377 2794 2228 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10399 BssSI C'ACGA_G Cuts at: 2539 3595 6686 8459 9471 2539 Size: 1056 3091 1773 1012 3467 Fragments arranged by size: 3467 3091 1773 1056 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2774 3535 5155 6645 7399 794 Size: 1980 761 1620 1490 754 3794 Fragments arranged by size: 3794 1980 1620 1490 761 754 BstBI TT'CG_AA Cuts at: 9203 9203 Size: 10399 BstEII G'GTnAC_C Cuts at: 1184 3679 4240 6406 1184 Size: 2495 561 2166 5177 Fragments arranged by size: 5177 2495 2166 561 BstXI CCAn_nnnn'nTGG Cuts at: 2419 3331 4777 5005 6470 7075 2419 Size: 912 1446 228 1465 605 5743 Fragments arranged by size: 5743 1465 1446 912 605 228 BstYI r'GATC_y Cuts at: 1972 2280 3585 4224 7729 8096 8481 8498 9950 Size: 308 1305 639 3505 367 385 17 1452 Cuts at: 9950 10036 10048 10394 1972 Size: 86 12 346 1977 Fragments arranged by size: 3505 1977 1452 1305 639 385 367 346 308 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2121 3612 2121 Size: 1491 8908 BtgI C'CryG_G Cuts at: 513 1065 2004 2086 3426 3483 3549 3867 3933 Size: 552 939 82 1340 57 66 318 66 Cuts at: 3933 3964 4139 4228 4273 4467 4833 5215 5670 Size: 31 175 89 45 194 366 382 455 Cuts at: 5670 6267 6376 6526 6606 6891 7036 7617 513 Size: 597 109 150 80 285 145 581 3295 Fragments arranged by size: 3295 1340 939 597 581 552 455 382 366 318 285 194 175 150 145 109 89 82 80 66 66 57 45 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3504 5797 6115 6223 487 Size: 683 56 2278 2293 318 108 4663 Fragments arranged by size: 4663 2293 2278 683 318 108 56 BtsI GCAGTG_nn' Cuts at: 2599 2775 3219 3308 4170 4479 6616 7489 7501 Size: 176 444 89 862 309 2137 873 12 Cuts at: 7501 7915 8709 8729 2599 Size: 414 794 20 4269 Fragments arranged by size: 4269 2137 873 862 794 444 414 309 176 89 20 12 ClaI AT'CG_AT Cuts at: 1554 6168 6210 1554 Size: 4614 42 5743 Fragments arranged by size: 5743 4614 42 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10364 DraI TTT'AAA Cuts at: 7703 7703 Size: 10399 DraIII CAC_nnn'GTG Cuts at: 3679 5248 3679 Size: 1569 8830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2137 4937 9406 809 Size: 507 821 2800 4469 1802 Fragments arranged by size: 4469 2800 1802 821 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2548 2845 2901 Size: 54 1425 50 275 18 718 297 56 Cuts at: 2901 3111 4219 4470 5317 6306 6368 6432 6518 Size: 210 1108 251 847 989 62 64 86 Cuts at: 6518 6709 6808 6915 7235 7385 7846 8735 10364 Size: 191 99 107 320 150 461 889 1629 Cuts at: 10364 8 Size: 43 Fragments arranged by size: 1629 1425 1108 989 889 847 718 461 320 297 275 251 210 191 150 107 99 86 64 62 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2548 5317 7846 1537 Size: 275 736 2769 2529 4090 Fragments arranged by size: 4090 2769 2529 736 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2376 2961 8329 1353 Size: 1023 585 5368 3423 Fragments arranged by size: 5368 3423 1023 585 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2496 3814 4817 5499 6005 8986 Size: 1064 138 907 1318 1003 682 506 2981 Cuts at: 8986 9360 9506 387 Size: 374 146 1280 Fragments arranged by size: 2981 1318 1280 1064 1003 907 682 506 374 146 138 EcoICRI GAG'CTC Cuts at: 2818 2863 6898 2818 Size: 45 4035 6319 Fragments arranged by size: 6319 4035 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2879 3229 3740 3988 4046 4277 Size: 393 821 426 350 511 248 58 231 Cuts at: 4277 4475 5468 5546 5677 5903 6113 6301 6353 Size: 198 993 78 131 226 210 188 52 Cuts at: 6353 6460 6812 6855 7565 7685 8462 9059 9846 Size: 107 352 43 710 120 777 597 787 Cuts at: 9846 10114 1239 Size: 268 1524 Fragments arranged by size: 1524 993 821 787 777 710 597 511 426 393 352 350 268 248 231 226 210 198 188 131 120 107 78 58 52 43 EcoNI CCTnn'n_nnAGG Cuts at: 4625 6401 4625 Size: 1776 8623 EcoO109I rG'GnC_Cy Cuts at: 2423 3085 3086 3715 3886 4012 4076 4704 4714 Size: 662 1 629 171 126 64 628 10 Cuts at: 4714 4786 5233 5774 5841 6250 6251 6602 7118 Size: 72 447 541 67 409 1 351 516 Cuts at: 7118 2423 Size: 5704 Fragments arranged by size: 5704 662 629 628 541 516 447 409 351 171 126 72 67 64 10 1 1 EcoRI G'AATT_C Cuts at: 6082 7708 8086 6082 Size: 1626 378 8395 Fragments arranged by size: 8395 1626 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3700 5908 3700 Size: 2208 8191 FseI GG_CCGG'CC Cuts at: 4447 4447 Size: 10399 FspI TGC'GCA Cuts at: 4109 6449 8905 4109 Size: 2340 2456 5603 Fragments arranged by size: 5603 2456 2340 FspAI rTGC'GCAy Cuts at: 4109 6449 4109 Size: 2340 8059 HaeII r_GCGC'y Cuts at: 1678 2085 2236 3242 3285 5686 6007 6788 6954 Size: 407 151 1006 43 2401 321 781 166 Cuts at: 6954 7249 9546 1678 Size: 295 2297 2531 Fragments arranged by size: 2531 2401 2297 1006 781 407 321 295 166 151 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3333 3365 3557 3589 3680 Size: 32 135 32 1761 32 192 32 91 Cuts at: 3680 3712 4269 4301 5135 5167 5833 5855 5865 Size: 32 557 32 834 32 666 22 10 Cuts at: 5865 5887 6032 6064 6326 6358 7334 7366 9040 Size: 22 145 32 262 32 976 32 1674 Cuts at: 9040 9072 9114 9146 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1761 1674 976 834 666 557 262 192 145 135 91 42 32 32 32 32 32 32 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 7276 7573 7743 7989 669 Size: 988 281 5338 297 170 246 3079 Fragments arranged by size: 5338 3079 988 297 281 246 170 HindIII A'AGCT_T Cuts at: 748 7196 748 Size: 6448 3951 HpaI GTT'AAC Cuts at: 7989 7989 Size: 10399 KasI G'GCGC_C Cuts at: 2081 3238 2081 Size: 1157 9242 KpnI G_GTAC'C Cuts at: 5390 7724 5390 Size: 2334 8065 MfeI C'AATT_G Cuts at: 7998 7998 Size: 10399 MluI A'CGCG_T Cuts at: 10275 10275 Size: 10399 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2628 3555 6396 6651 9513 9697 1305 Size: 1323 927 2841 255 2862 184 2007 Fragments arranged by size: 2862 2841 2007 1323 927 255 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2903 3113 4221 6370 6434 Size: 54 1425 343 1071 210 1108 2149 64 Cuts at: 6434 6520 6711 6917 7237 7387 10 Size: 86 191 206 320 150 3022 Fragments arranged by size: 3022 2149 1425 1108 1071 343 320 210 206 191 150 86 64 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2417 2897 3296 3425 3482 4070 Size: 648 1035 216 480 399 129 57 588 Cuts at: 4070 4175 4247 4454 4472 4835 5003 5381 6468 Size: 105 72 207 18 363 168 378 1087 Cuts at: 6468 6611 6818 7184 7494 7517 8357 8716 8875 Size: 143 207 366 310 23 840 359 159 Cuts at: 8875 518 Size: 2042 Fragments arranged by size: 2042 1087 1035 840 648 588 480 399 378 366 363 359 310 216 207 207 168 159 143 129 105 72 57 23 18 MspA1I CmG'CkG Cuts at: 1606 2392 2431 2563 3074 3551 3748 3966 4008 Size: 786 39 132 511 477 197 218 42 Cuts at: 4008 4131 4141 4230 4332 4725 5070 5099 5290 Size: 123 10 89 102 393 345 29 191 Cuts at: 5290 5497 7645 7815 8492 9640 9885 1606 Size: 207 2148 170 677 1148 245 2120 Fragments arranged by size: 2148 2120 1148 786 677 511 477 393 345 245 218 207 197 191 170 132 123 102 89 42 39 29 10 NaeI GCC'GGC Cuts at: 4445 4445 Size: 10399 NarI GG'CG_CC Cuts at: 2082 3239 2082 Size: 1157 9242 NcoI C'CATG_G Cuts at: 513 1065 2004 2086 5670 6267 6526 6606 6891 Size: 552 939 82 3584 597 259 80 285 Cuts at: 6891 7617 513 Size: 726 3295 Fragments arranged by size: 3584 3295 939 726 597 552 285 259 82 80 NdeI CA'TA_TG Cuts at: 387 5533 387 Size: 5146 5253 NgoMIV G'CCGG_C Cuts at: 4443 4443 Size: 10399 NheI G'CTAG_C Cuts at: 1052 4303 1052 Size: 3251 7148 NotI GC'GGCC_GC Cuts at: 7846 7846 Size: 10399 NspI r_CATG'y Cuts at: 1132 1229 2763 3277 3977 4184 4748 5416 6727 Size: 97 1534 514 700 207 564 668 1311 Cuts at: 6727 7156 7300 7555 7579 7757 9302 1132 Size: 429 144 255 24 178 1545 2229 Fragments arranged by size: 2229 1545 1534 1311 700 668 564 514 429 255 207 178 144 97 24 PasI CC'CwG_GG Cuts at: 1348 2024 2399 1348 Size: 676 375 9348 Fragments arranged by size: 9348 676 375 PciI A'CATG_T Cuts at: 7575 9298 7575 Size: 1723 8676 PflMI CCAn_nnn'nTGG Cuts at: 1108 6377 6527 6856 1108 Size: 5269 150 329 4651 Fragments arranged by size: 5269 4651 329 150 PfoI T'CCnGG_A Cuts at: 2856 4991 5559 7074 7542 2856 Size: 2135 568 1515 468 5713 Fragments arranged by size: 5713 2135 1515 568 468 PmeI GTTT'AAAC Cuts at: 7703 7703 Size: 10399 PmlI CAC'GTG Cuts at: 6075 6715 6804 6075 Size: 640 89 9670 Fragments arranged by size: 9670 640 89 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6887 6919 8468 8500 9981 10013 6887 Size: 32 1549 32 1481 32 7273 Fragments arranged by size: 7273 1549 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3715 3886 4012 4704 4714 4786 5774 5841 3715 Size: 171 126 692 10 72 988 67 8273 Fragments arranged by size: 8273 988 692 171 126 72 67 10 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10399 PsiI TTA'TAA Cuts at: 7969 7969 Size: 10399 PspOMI G'GGCC_C Cuts at: 3085 4075 6250 6602 3085 Size: 990 2175 352 6882 Fragments arranged by size: 6882 2175 990 352 PspXI vC'TCGA_Gb Cuts at: 6899 6899 Size: 10399 PstI C_TGCA'G Cuts at: 830 4545 4872 5181 5241 6623 7029 7496 7751 Size: 3715 327 309 60 1382 406 467 255 Cuts at: 7751 830 Size: 3478 Fragments arranged by size: 3715 3478 1382 467 406 327 309 255 60 PvuI CG_AT'CG Cuts at: 2001 5877 2001 Size: 3876 6523 PvuII CAG'CTG Cuts at: 2392 2431 2563 3748 4008 5070 5099 7645 2392 Size: 39 132 1185 260 1062 29 2546 5146 Fragments arranged by size: 5146 2546 1185 1062 260 132 39 29 RsrII CG'GwC_CG Cuts at: 3871 3871 Size: 10399 SacI G_AGCT'C Cuts at: 2820 2865 6900 2820 Size: 45 4035 6319 Fragments arranged by size: 6319 4035 45 SacII CC_GC'GG Cuts at: 3552 3967 4142 4231 3552 Size: 415 175 89 9720 Fragments arranged by size: 9720 415 175 89 SalI G'TCGA_C Cuts at: 1936 7571 7741 1936 Size: 5635 170 4594 Fragments arranged by size: 5635 4594 170 SanDI GG'GwC_CC Cuts at: 3886 4786 5774 3886 Size: 900 988 8511 Fragments arranged by size: 8511 988 900 SapI GCTCTTCn'nnn_ Cuts at: 2961 2961 Size: 10399 SbfI CC_TGCA'GG Cuts at: 7751 7751 Size: 10399 ScaI AGT'ACT Cuts at: 3929 4547 5195 6365 7232 7265 8647 10359 3929 Size: 618 648 1170 867 33 1382 1712 3969 Fragments arranged by size: 3969 1712 1382 1170 867 648 618 33 SexAI A'CCwGG_T Cuts at: 4014 4236 5244 4014 Size: 222 1008 9169 Fragments arranged by size: 9169 1008 222 SfcI C'TryA_G Cuts at: 826 1045 2172 2526 3124 3454 4541 4868 5177 Size: 219 1127 354 598 330 1087 327 309 Cuts at: 5177 5237 6619 7025 7492 7747 8882 9563 9754 Size: 60 1382 406 467 255 1135 681 191 Cuts at: 9754 826 Size: 1471 Fragments arranged by size: 1471 1382 1135 1127 1087 681 598 467 406 354 330 327 309 255 219 191 60 SfoI GGC'GCC Cuts at: 2083 3240 2083 Size: 1157 9242 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10399 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10399 SmaI CCC'GGG Cuts at: 1872 6181 6347 7340 7726 1872 Size: 4309 166 993 386 4545 Fragments arranged by size: 4545 4309 993 386 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 4910 5629 6026 6147 6899 8503 Size: 197 926 2967 719 397 121 752 1604 Cuts at: 8503 9404 9666 820 Size: 901 262 1553 Fragments arranged by size: 2967 1604 1553 926 901 752 719 397 262 197 121 SnaBI TAC'GTA Cuts at: 493 8190 493 Size: 7697 2702 SpeI A'CTAG_T Cuts at: 152 2357 152 Size: 2205 8194 SphI G_CATG'C Cuts at: 1132 3277 5416 6727 7156 7555 7757 1132 Size: 2145 2139 1311 429 399 202 3774 Fragments arranged by size: 3774 2145 2139 1311 429 399 202 SspI AAT'ATT Cuts at: 5 52 8323 5 Size: 47 8271 2081 Fragments arranged by size: 8271 2081 47 StuI AGG'CCT Cuts at: 1517 6581 7100 1517 Size: 5064 519 4816 Fragments arranged by size: 5064 4816 519 StyI C'CwwG_G Cuts at: 513 1065 2004 2086 2608 2823 4383 4506 5423 Size: 552 939 82 522 215 1560 123 917 Cuts at: 5423 5574 5670 6267 6526 6606 6891 6924 7452 Size: 151 96 597 259 80 285 33 528 Cuts at: 7452 7617 7838 513 Size: 165 221 3074 Fragments arranged by size: 3074 1560 939 917 597 552 528 522 285 259 221 215 165 151 123 96 82 80 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2333 2762 2794 3938 4042 4515 4519 Size: 1255 753 429 32 1144 104 473 4 Cuts at: 4519 4667 5755 5979 5983 8426 8443 8596 8781 Size: 148 1088 224 4 2443 17 153 185 Cuts at: 8781 9226 325 Size: 445 1498 Fragments arranged by size: 2443 1498 1255 1144 1088 753 473 445 429 224 185 153 148 104 32 17 4 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2324 3642 3927 Size: 274 80 33 51 1443 347 1318 285 Cuts at: 3927 4545 5118 5193 6363 7230 7263 7290 8645 Size: 618 573 75 1170 867 33 27 1355 Cuts at: 8645 10357 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1355 1318 1170 867 618 573 347 285 274 138 80 75 51 33 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5234 5266 5747 5779 5837 5869 10359 10391 5234 Size: 32 481 32 58 32 4490 32 5242 Fragments arranged by size: 5242 4490 481 58 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4762 1316 Size: 3446 6953 XbaI T'CTAG_A Cuts at: 5464 7735 5464 Size: 2271 8128 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2911 3292 4012 5398 5983 7039 2911 Size: 381 720 1386 585 1056 6271 Fragments arranged by size: 6271 1386 1056 720 585 381 XhoI C'TCGA_G Cuts at: 1943 6147 6899 1943 Size: 4204 752 5443 Fragments arranged by size: 5443 4204 752 XmaI C'CCGG_G Cuts at: 1870 6179 6345 7338 7724 1870 Size: 4309 166 993 386 4545 Fragments arranged by size: 4545 4309 993 386 166 XmnI GAAnn'nnTTC Cuts at: 8528 8528 Size: 10399 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4297 5055 8207 276 Size: 53 83 186 936 2763 758 3152 2468 Fragments arranged by size: 3152 2763 2468 936 758 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BplI BsmBI BstZ17I EcoRV NruI NsiI PacI PsrI SfiI SrfI SwaI