Restriction map for pFN21AB1440
 (Circular) (Six-base) MAPSORT of: pFN21AB1440.seq  Check: 5381  from: 1  to: 10399



pFN21AB1440  10399 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   7429    7429 
   Size:      10399
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4299    5057    8209     278 
   Size:         53      83     186     936    2763     758    3152    2468

  Fragments arranged by size: 

                3152    2763    2468     936     758     186      83      53
            
 
AccI GT'mk_AC

Cuts at:   1937    2529    3351    4185    4766    7572    7742    1937 
   Size:        592     822     834     581    2806     170    4594

  Fragments arranged by size: 

                4594    2806     834     822     592     581     170
 
Acc65I G'GTAC_C

Cuts at:   5386    7720    5386 
   Size:       2334    8065
 
AclI AA'CG_TT

Cuts at:   1563    8526    8899    1563 
   Size:       6963     373    3063

  Fragments arranged by size: 

                6963    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2453    3740    5546    5903    8462    9846   10114    1632 
   Size:        821    1287    1806     357    2559    1384     268    1917

  Fragments arranged by size: 

                2559    1917    1806    1384    1287     821     357     268
            
 
AfeI AGC'GCT

Cuts at:   2234    5684    7247    2234 
   Size:       3450    1563    5386

  Fragments arranged by size: 

                5386    3450    1563
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197   10202
 
AflIII A'CryG_T

Cuts at:   1203    2648    6768    6933    7575    9298   10275    1203 
   Size:       1445    4120     165     642    1723     977    1327

  Fragments arranged by size: 

                4120    1723    1445    1327     977     642     165
 
AgeI A'CCGG_T

Cuts at:   9230    9230 
   Size:      10399
 
AhdI GACnn_n'nnGTC

Cuts at:   9128    9128 
   Size:      10399
 
AleI CACnn'nnGTG

Cuts at:   1166    2897    3425    3482    4070    4175    4247    4835    6468 
   Size:       1731     528      57     588     105      72     588    1633

Cuts at:   6468    6818    7494    7517    1166 
   Size:        350     676      23    4048

  Fragments arranged by size: 

                4048    1731    1633     676     588     588     528     350
                 105      72      57      23
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3455    4115    5034    7512    1184 
   Size:       2271     660     919    2478    4071

  Fragments arranged by size: 

                4071    2478    2271     919     660
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6887    6919    6887 
   Size:         32   10367
 
AlwNI CAG_nnn'CTG

Cuts at:   2495    2663    4867    5245    6856    8142    9714    2495 
   Size:        168    2204     378    1611    1286    1572    3180

  Fragments arranged by size: 

                3180    2204    1611    1572    1286     378     168
 
ApaI G_GGCC'C

Cuts at:   3089    4079    6254    6606    3089 
   Size:        990    2175     352    6882

  Fragments arranged by size: 

                6882    2175     990     352
 
ApaLI G'TGCA_C

Cuts at:   2775    6614    7487    8456    9612    2775 
   Size:       3839     873     969    1156    3562

  Fragments arranged by size: 

                3839    3562    1156     969     873
 
ApoI r'AATT_y

Cuts at:   1493    6082    7708    7934    8086   10195    1493 
   Size:       4589    1626     226     152    2109    1697

  Fragments arranged by size: 

                4589    2109    1697    1626     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    8953     160 
   Size:        872    7921    1606

  Fragments arranged by size: 

                7921    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10399
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3314    4085    4709    5438    6147    6179    6260 
   Size:         73    1371     771     624     729     709      32      81

Cuts at:   6260    6345    6899    7338    7724    1870 
   Size:         85     554     439     386    4545

  Fragments arranged by size: 

                4545    1371     771     729     709     624     554     439
                 386      85      81      73      32
 
BamHI G'GATC_C

Cuts at:   2280    3585    7729    8096    2280 
   Size:       1305    4144     367    4583

  Fragments arranged by size: 

                4583    4144    1305     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2038    2081    2573    3238    4394 
   Size:        325     217     627     251      43     492     665    1156

Cuts at:   4394    4497    5386    6380    6820    7720    9175     618 
   Size:        103     889     994     440     900    1455    1842

  Fragments arranged by size: 

                1842    1455    1156     994     900     889     665     627
                 492     440     325     251     217     103      43
 
BanII G_rGCy'C

Cuts at:   1391    2820    2865    3089    3324    3417    3863    4079    4272 
   Size:       1429      45     224     235      93     446     216     193

Cuts at:   4272    6254    6606    6900    7052    7718    1391 
   Size:       1982     352     294     152     666    4072

  Fragments arranged by size: 

                4072    1982    1429     666     446     352     294     235
                 224     216     193     152      93      45
 
BbeI G_GCGC'C

Cuts at:   2085    3242    2085 
   Size:       1157    9242
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2098    3669    3772    5057    6698    7598     928 
   Size:        971     199    1571     103    1285    1641     900    3729

  Fragments arranged by size: 

                3729    1641    1571    1285     971     900     199     103
            
 
BbvCI CC'TCA_GC

Cuts at:   2427    3140    2427 
   Size:        713    9686
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    8590    8624    1903 
   Size:         34    6653      34    3678

  Fragments arranged by size: 

                6653    3678      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3335    8291    9501    3335 
   Size:       4956    1210    4233

  Fragments arranged by size: 

                4956    4233    1210
 
BclI T'GATC_A

Cuts at:   1548    4458    6358    1548 
   Size:       2910    1900    5589

  Fragments arranged by size: 

                5589    2910    1900
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3912    3944    4132 
   Size:        107     122      71     631     605    2240      32     188

Cuts at:   4132    4562    6642    9010     136 
   Size:        430    2080    2368    1525

  Fragments arranged by size: 

                2368    2240    2080    1525     631     605     430     188
                 122     107      71      32
 
BglII A'GATC_T

Cuts at:  10394   10394 
   Size:      10399
 
BlpI GC'TnA_GC

Cuts at:   2864    3278    5066    7816    2864 
   Size:        414    1788    2750    5447

  Fragments arranged by size: 

                5447    2750    1788     414
 
Bme1580I G_kGCm'C

Cuts at:   1790    2043    2779    3080    3089    3223    4063    4079    4128 
   Size:        253     736     301       9     134     840      16      49

Cuts at:   4128    4399    5106    5151    6254    6606    6618    7404    7491 
   Size:        271     707      45    1103     352      12     786      87

Cuts at:   7491    8460    9616    1790 
   Size:        969    1156    2573

  Fragments arranged by size: 

                2573    1156    1103     969     840     786     736     707
                 352     301     271     253     134      87      49      45
                  16      12       9
 
BmgBI CAC'GTC

Cuts at:   1318    4072    4123    4764    6936    1318 
   Size:       2754      51     641    2172    4781

  Fragments arranged by size: 

                4781    2754    2172     641      51
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2142    3655    3805 
   Size:        416      53      83     399      24     725    1513     150

Cuts at:   3805    3919    7297    8130    9088     442 
   Size:        114    3378     833     958    1753

  Fragments arranged by size: 

                3378    1753    1513     958     833     725     416     399
                 150     114      83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    4307    1056 
   Size:       3251    7148
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2879    3229    3988    4046    4277    4475    5468    5677 
   Size:       1640     350     759      58     231     198     993     209

Cuts at:   5677    6113    6301    6353    6460    6812    6855    7565    7685 
   Size:        436     188      52     107     352      43     710     120

Cuts at:   7685    9059    1239 
   Size:       1374    2579

  Fragments arranged by size: 

                2579    1640    1374     993     759     710     436     352
                 350     231     209     198     188     120     107      58
                  52      43
 
Bpu10I CC'TnA_GC

Cuts at:   2013    2427    3140    2013 
   Size:        414     713    9272

  Fragments arranged by size: 

                9272     713     414
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4895    5650    6011    8524    9389    9687    4895 
   Size:        755     361    2513     865     298    5607

  Fragments arranged by size: 

                5607    2513     865     755     361     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4340    5952    6257    6380    9062     882 
   Size:        617    2841    1612     305     123    2682    2219

  Fragments arranged by size: 

                2841    2682    2219    1612     617     305     123
 
BsaAI yAC'GTr

Cuts at:    493    1204    2791    4249    6075    6715    6769    6804    8190 
   Size:        711    1587    1458    1826     640      54      35    1386

Cuts at:   8190     493 
   Size:       2702

  Fragments arranged by size: 

                2702    1826    1587    1458    1386     711     640      54
                  35
 
BsaBI GATnn'nnATC

Cuts at:  10393   10393 
   Size:      10399
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2082    3239    3777    4296 
   Size:         53      83     186     936     549    1157     538     519

Cuts at:   4296    5054    5420    6174    8206    8588    9223     275 
   Size:        758     366     754    2032     382     635    1451

  Fragments arranged by size: 

                2032    1451    1157     936     758     754     635     549
                 538     519     382     366     186      83      53
 
BsaWI w'CCGG_w

Cuts at:   3361    5623    8832    9230    9504    9651   10062    3361 
   Size:       2262    3209     398     274     147     411    3698

  Fragments arranged by size: 

                3698    3209    2262     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3680    3710    5000    5030    5747 
   Size:         30     165      30    2277      30    1290      30     717

Cuts at:   5747    5777    5833    5863    6032    6062    6326    6356    6749 
   Size:         30      56      30     169      30     264      30     393

Cuts at:   6749    6779    6887    6917    1178 
   Size:         30     108      30    4660

  Fragments arranged by size: 

                4660    2277    1290     717     393     264     169     165
                 108      56      30      30      30      30      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2688    2828    2899    3332    3407    3688    4697    5504 
   Size:       1502     140      71     433      75     281    1009     807

Cuts at:   5504    5762    5896    6350    1186 
   Size:        258     134     454    5235

  Fragments arranged by size: 

                5235    1502    1009     807     454     433     281     258
                 140     134      75      71
 
BseYI C'CCAG_C

Cuts at:   2229    2717    2914    3203    3500    3543    4008    4127    4205 
   Size:        488     197     289     297      43     465     119      78

Cuts at:   4205    4528    4721    4979    5070    5736    6856    9602   10098 
   Size:        323     193     258      91     666    1120    2746     496

Cuts at:  10098    2229 
   Size:       2530

  Fragments arranged by size: 

                2746    2530    1120     666     496     488     465     323
                 297     289     258     197     193     119      91      78
                  43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2037    2318    3284    3320    3738    4452    4607 
   Size:        573     301     281     966      36     418     714     155

Cuts at:   4607    6770    7643    1163 
   Size:       2163     873    3919

  Fragments arranged by size: 

                3919    2163     966     873     714     573     418     301
                 281     155      36
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2551    3426    5320    5877    7849    8610 
   Size:        275     186     550     875    1894     557    1972     761

Cuts at:   8610    9638    1540 
   Size:       1028    2301

  Fragments arranged by size: 

                2301    1972    1894    1028     875     761     557     550
                 275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2379    2779    2820    2865    4002    5435    5559    6618    6900 
   Size:        400      41      45    1137    1433     124    1059     282

Cuts at:   6900    7136    7167    7491    8460    8545    9616    2379 
   Size:        236      31     324     969      85    1071    3162

  Fragments arranged by size: 

                3162    1433    1137    1071    1059     969     400     324
                 282     236     124      85      45      41      31
 
BsiWI C'GTAC_G

Cuts at:   2875    2875 
   Size:      10399
 
BsmI GAATG_Cn'

Cuts at:   6235    7910    8003    6235 
   Size:       1675      93    8631

  Fragments arranged by size: 

                8631    1675      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2043    2379    2779    2820    2865    3080    3089 
   Size:        399     253     336     400      41      45     215       9

Cuts at:   3089    3223    3324    3417    3863    4002    4063    4079    4128 
   Size:        134     101      93     446     139      61      16      49

Cuts at:   4128    4272    4399    5106    5151    5435    5559    6254    6606 
   Size:        144     127     707      45     284     124     695     352

Cuts at:   6606    6618    6900    7052    7136    7167    7404    7491    7718 
   Size:         12     282     152      84      31     237      87     227

Cuts at:   7718    8460    8545    9616    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     707     695     446     400     399
                 352     336     284     282     253     237     227     215
                 152     144     139     134     127     124     101      93
                  87      85      84      61      49      45      45      41
                  31      16      12       9
 
BspEI T'CCGG_A

Cuts at:   3361    5623   10062    3361 
   Size:       2262    4439    3698

  Fragments arranged by size: 

                4439    3698    2262
 
BspHI T'CATG_A

Cuts at:   6318    8288   10018    6318 
   Size:       1970    1730    6699

  Fragments arranged by size: 

                6699    1970    1730
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    4725    4749    5184    6728    6966    7429    7754     844 
   Size:       3881      24     435    1544     238     463     325    3489

  Fragments arranged by size: 

                3881    3489    1544     463     435     325     238      24
            
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3619    6676    8286    1394 
   Size:        429    1796    3057    1610    3507

  Fragments arranged by size: 

                3507    3057    1796    1610     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2288    2875    5052    8894    9068      65 
   Size:       1183    1040     587    2177    3842     174    1396

  Fragments arranged by size: 

                3842    2177    1396    1183    1040     587     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4443    4536    4616    5002    5610    6641    6981 
   Size:        110    2633      93      80     386     608    1031     340

Cuts at:   6981    9043    9230    1700 
   Size:       2062     187    2869

  Fragments arranged by size: 

                2869    2633    2062    1031     608     386     340     187
                 110      93      80
 
BsrGI T'GTAC_A

Cuts at:     96    2324    5118      96 
   Size:       2228    2794    5377

  Fragments arranged by size: 

                5377    2794    2228
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:      10399
 
BssSI C'ACGA_G

Cuts at:   2539    3595    6686    8459    9471    2539 
   Size:       1056    3091    1773    1012    3467

  Fragments arranged by size: 

                3467    3091    1773    1056    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2774    3535    5155    6645    7399     794 
   Size:       1980     761    1620    1490     754    3794

  Fragments arranged by size: 

                3794    1980    1620    1490     761     754
 
BstBI TT'CG_AA

Cuts at:   9203    9203 
   Size:      10399
 
BstEII G'GTnAC_C

Cuts at:   1184    3679    4240    6406    1184 
   Size:       2495     561    2166    5177

  Fragments arranged by size: 

                5177    2495    2166     561
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2419    3331    4777    5005    6470    7075    2419 
   Size:        912    1446     228    1465     605    5743

  Fragments arranged by size: 

                5743    1465    1446     912     605     228
 
BstYI r'GATC_y

Cuts at:   1972    2280    3585    4224    7729    8096    8481    8498    9950 
   Size:        308    1305     639    3505     367     385      17    1452

Cuts at:   9950   10036   10048   10394    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                3505    1977    1452    1305     639     385     367     346
                 308      86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2121    3612    2121 
   Size:       1491    8908
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2086    3426    3483    3549    3867    3933 
   Size:        552     939      82    1340      57      66     318      66

Cuts at:   3933    3964    4139    4228    4273    4467    4833    5215    5670 
   Size:         31     175      89      45     194     366     382     455

Cuts at:   5670    6267    6376    6526    6606    6891    7036    7617     513 
   Size:        597     109     150      80     285     145     581    3295

  Fragments arranged by size: 

                3295    1340     939     597     581     552     455     382
                 366     318     285     194     175     150     145     109
                  89      82      80      66      66      57      45      31
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    3504    5797    6115    6223     487 
   Size:        683      56    2278    2293     318     108    4663

  Fragments arranged by size: 

                4663    2293    2278     683     318     108      56
 
BtsI GCAGTG_nn'

Cuts at:   2599    2775    3219    3308    4170    4479    6616    7489    7501 
   Size:        176     444      89     862     309    2137     873      12

Cuts at:   7501    7915    8709    8729    2599 
   Size:        414     794      20    4269

  Fragments arranged by size: 

                4269    2137     873     862     794     444     414     309
                 176      89      20      12
 
ClaI AT'CG_AT

Cuts at:   1554    6168    6210    1554 
   Size:       4614      42    5743

  Fragments arranged by size: 

                5743    4614      42
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35   10364
 
DraI TTT'AAA

Cuts at:   7703    7703 
   Size:      10399
 
DraIII CAC_nnn'GTG

Cuts at:   3679    5248    3679 
   Size:       1569    8830
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2137    4937    9406     809 
   Size:        507     821    2800    4469    1802

  Fragments arranged by size: 

                4469    2800    1802     821     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2548    2845    2901 
   Size:         54    1425      50     275      18     718     297      56

Cuts at:   2901    3111    4219    4470    5317    6306    6368    6432    6518 
   Size:        210    1108     251     847     989      62      64      86

Cuts at:   6518    6709    6808    6915    7235    7385    7846    8735   10364 
   Size:        191      99     107     320     150     461     889    1629

Cuts at:  10364       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1108     989     889     847     718     461
                 320     297     275     251     210     191     150     107
                  99      86      64      62      56      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2548    5317    7846    1537 
   Size:        275     736    2769    2529    4090

  Fragments arranged by size: 

                4090    2769    2529     736     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2376    2961    8329    1353 
   Size:       1023     585    5368    3423

  Fragments arranged by size: 

                5368    3423    1023     585
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2496    3814    4817    5499    6005    8986 
   Size:       1064     138     907    1318    1003     682     506    2981

Cuts at:   8986    9360    9506     387 
   Size:        374     146    1280

  Fragments arranged by size: 

                2981    1318    1280    1064    1003     907     682     506
                 374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2818    2863    6898    2818 
   Size:         45    4035    6319

  Fragments arranged by size: 

                6319    4035      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2453    2879    3229    3740    3988    4046    4277 
   Size:        393     821     426     350     511     248      58     231

Cuts at:   4277    4475    5468    5546    5677    5903    6113    6301    6353 
   Size:        198     993      78     131     226     210     188      52

Cuts at:   6353    6460    6812    6855    7565    7685    8462    9059    9846 
   Size:        107     352      43     710     120     777     597     787

Cuts at:   9846   10114    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     993     821     787     777     710     597     511
                 426     393     352     350     268     248     231     226
                 210     198     188     131     120     107      78      58
                  52      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:   4625    6401    4625 
   Size:       1776    8623
 
EcoO109I rG'GnC_Cy

Cuts at:   2423    3085    3086    3715    3886    4012    4076    4704    4714 
   Size:        662       1     629     171     126      64     628      10

Cuts at:   4714    4786    5233    5774    5841    6250    6251    6602    7118 
   Size:         72     447     541      67     409       1     351     516

Cuts at:   7118    2423 
   Size:       5704

  Fragments arranged by size: 

                5704     662     629     628     541     516     447     409
                 351     171     126      72      67      64      10       1
                   1
 
EcoRI G'AATT_C

Cuts at:   6082    7708    8086    6082 
   Size:       1626     378    8395

  Fragments arranged by size: 

                8395    1626     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3700    5908    3700 
   Size:       2208    8191
 
FseI GG_CCGG'CC

Cuts at:   4447    4447 
   Size:      10399
 
FspI TGC'GCA

Cuts at:   4109    6449    8905    4109 
   Size:       2340    2456    5603

  Fragments arranged by size: 

                5603    2456    2340
 
FspAI rTGC'GCAy

Cuts at:   4109    6449    4109 
   Size:       2340    8059
 
HaeII r_GCGC'y

Cuts at:   1678    2085    2236    3242    3285    5686    6007    6788    6954 
   Size:        407     151    1006      43    2401     321     781     166

Cuts at:   6954    7249    9546    1678 
   Size:        295    2297    2531

  Fragments arranged by size: 

                2531    2401    2297    1006     781     407     321     295
                 166     151      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3333    3365    3557    3589    3680 
   Size:         32     135      32    1761      32     192      32      91

Cuts at:   3680    3712    4269    4301    5135    5167    5833    5855    5865 
   Size:         32     557      32     834      32     666      22      10

Cuts at:   5865    5887    6032    6064    6326    6358    7334    7366    9040 
   Size:         22     145      32     262      32     976      32    1674

Cuts at:   9040    9072    9114    9146    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1761    1674     976     834     666     557     262
                 192     145     135      91      42      32      32      32
                  32      32      32      32      32      32      32      32
                  32      22      22      10
 
HincII GTy'rAC

Cuts at:    669    1657    1938    7276    7573    7743    7989     669 
   Size:        988     281    5338     297     170     246    3079

  Fragments arranged by size: 

                5338    3079     988     297     281     246     170
 
HindIII A'AGCT_T

Cuts at:    748    7196     748 
   Size:       6448    3951
 
HpaI GTT'AAC

Cuts at:   7989    7989 
   Size:      10399
 
KasI G'GCGC_C

Cuts at:   2081    3238    2081 
   Size:       1157    9242
 
KpnI G_GTAC'C

Cuts at:   5390    7724    5390 
   Size:       2334    8065
 
MfeI C'AATT_G

Cuts at:   7998    7998 
   Size:      10399
 
MluI A'CGCG_T

Cuts at:  10275   10275 
   Size:      10399
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2628    3555    6396    6651    9513    9697    1305 
   Size:       1323     927    2841     255    2862     184    2007

  Fragments arranged by size: 

                2862    2841    2007    1323     927     255     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2903    3113    4221    6370    6434 
   Size:         54    1425     343    1071     210    1108    2149      64

Cuts at:   6434    6520    6711    6917    7237    7387      10 
   Size:         86     191     206     320     150    3022

  Fragments arranged by size: 

                3022    2149    1425    1108    1071     343     320     210
                 206     191     150      86      64      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2201    2417    2897    3296    3425    3482    4070 
   Size:        648    1035     216     480     399     129      57     588

Cuts at:   4070    4175    4247    4454    4472    4835    5003    5381    6468 
   Size:        105      72     207      18     363     168     378    1087

Cuts at:   6468    6611    6818    7184    7494    7517    8357    8716    8875 
   Size:        143     207     366     310      23     840     359     159

Cuts at:   8875     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1087    1035     840     648     588     480     399
                 378     366     363     359     310     216     207     207
                 168     159     143     129     105      72      57      23
                  18
 
MspA1I CmG'CkG

Cuts at:   1606    2392    2431    2563    3074    3551    3748    3966    4008 
   Size:        786      39     132     511     477     197     218      42

Cuts at:   4008    4131    4141    4230    4332    4725    5070    5099    5290 
   Size:        123      10      89     102     393     345      29     191

Cuts at:   5290    5497    7645    7815    8492    9640    9885    1606 
   Size:        207    2148     170     677    1148     245    2120

  Fragments arranged by size: 

                2148    2120    1148     786     677     511     477     393
                 345     245     218     207     197     191     170     132
                 123     102      89      42      39      29      10
 
NaeI GCC'GGC

Cuts at:   4445    4445 
   Size:      10399
 
NarI GG'CG_CC

Cuts at:   2082    3239    2082 
   Size:       1157    9242
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2086    5670    6267    6526    6606    6891 
   Size:        552     939      82    3584     597     259      80     285

Cuts at:   6891    7617     513 
   Size:        726    3295

  Fragments arranged by size: 

                3584    3295     939     726     597     552     285     259
                  82      80
 
NdeI CA'TA_TG

Cuts at:    387    5533     387 
   Size:       5146    5253
 
NgoMIV G'CCGG_C

Cuts at:   4443    4443 
   Size:      10399
 
NheI G'CTAG_C

Cuts at:   1052    4303    1052 
   Size:       3251    7148
 
NotI GC'GGCC_GC

Cuts at:   7846    7846 
   Size:      10399
 
NspI r_CATG'y

Cuts at:   1132    1229    2763    3277    3977    4184    4748    5416    6727 
   Size:         97    1534     514     700     207     564     668    1311

Cuts at:   6727    7156    7300    7555    7579    7757    9302    1132 
   Size:        429     144     255      24     178    1545    2229

  Fragments arranged by size: 

                2229    1545    1534    1311     700     668     564     514
                 429     255     207     178     144      97      24
 
PasI CC'CwG_GG

Cuts at:   1348    2024    2399    1348 
   Size:        676     375    9348

  Fragments arranged by size: 

                9348     676     375
 
PciI A'CATG_T

Cuts at:   7575    9298    7575 
   Size:       1723    8676
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    6377    6527    6856    1108 
   Size:       5269     150     329    4651

  Fragments arranged by size: 

                5269    4651     329     150
 
PfoI T'CCnGG_A

Cuts at:   2856    4991    5559    7074    7542    2856 
   Size:       2135     568    1515     468    5713

  Fragments arranged by size: 

                5713    2135    1515     568     468
 
PmeI GTTT'AAAC

Cuts at:   7703    7703 
   Size:      10399
 
PmlI CAC'GTG

Cuts at:   6075    6715    6804    6075 
   Size:        640      89    9670

  Fragments arranged by size: 

                9670     640      89
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6887    6919    8468    8500    9981   10013    6887 
   Size:         32    1549      32    1481      32    7273

  Fragments arranged by size: 

                7273    1549    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3715    3886    4012    4704    4714    4786    5774    5841    3715 
   Size:        171     126     692      10      72     988      67    8273

  Fragments arranged by size: 

                8273     988     692     171     126      72      67      10
            
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:      10399
 
PsiI TTA'TAA

Cuts at:   7969    7969 
   Size:      10399
 
PspOMI G'GGCC_C

Cuts at:   3085    4075    6250    6602    3085 
   Size:        990    2175     352    6882

  Fragments arranged by size: 

                6882    2175     990     352
 
PspXI vC'TCGA_Gb

Cuts at:   6899    6899 
   Size:      10399
 
PstI C_TGCA'G

Cuts at:    830    4545    4872    5181    5241    6623    7029    7496    7751 
   Size:       3715     327     309      60    1382     406     467     255

Cuts at:   7751     830 
   Size:       3478

  Fragments arranged by size: 

                3715    3478    1382     467     406     327     309     255
                  60
 
PvuI CG_AT'CG

Cuts at:   2001    5877    2001 
   Size:       3876    6523
 
PvuII CAG'CTG

Cuts at:   2392    2431    2563    3748    4008    5070    5099    7645    2392 
   Size:         39     132    1185     260    1062      29    2546    5146

  Fragments arranged by size: 

                5146    2546    1185    1062     260     132      39      29
            
 
RsrII CG'GwC_CG

Cuts at:   3871    3871 
   Size:      10399
 
SacI G_AGCT'C

Cuts at:   2820    2865    6900    2820 
   Size:         45    4035    6319

  Fragments arranged by size: 

                6319    4035      45
 
SacII CC_GC'GG

Cuts at:   3552    3967    4142    4231    3552 
   Size:        415     175      89    9720

  Fragments arranged by size: 

                9720     415     175      89
 
SalI G'TCGA_C

Cuts at:   1936    7571    7741    1936 
   Size:       5635     170    4594

  Fragments arranged by size: 

                5635    4594     170
 
SanDI GG'GwC_CC

Cuts at:   3886    4786    5774    3886 
   Size:        900     988    8511

  Fragments arranged by size: 

                8511     988     900
 
SapI GCTCTTCn'nnn_

Cuts at:   2961    2961 
   Size:      10399
 
SbfI CC_TGCA'GG

Cuts at:   7751    7751 
   Size:      10399
 
ScaI AGT'ACT

Cuts at:   3929    4547    5195    6365    7232    7265    8647   10359    3929 
   Size:        618     648    1170     867      33    1382    1712    3969

  Fragments arranged by size: 

                3969    1712    1382    1170     867     648     618      33
            
 
SexAI A'CCwGG_T

Cuts at:   4014    4236    5244    4014 
   Size:        222    1008    9169

  Fragments arranged by size: 

                9169    1008     222
 
SfcI C'TryA_G

Cuts at:    826    1045    2172    2526    3124    3454    4541    4868    5177 
   Size:        219    1127     354     598     330    1087     327     309

Cuts at:   5177    5237    6619    7025    7492    7747    8882    9563    9754 
   Size:         60    1382     406     467     255    1135     681     191

Cuts at:   9754     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1382    1135    1127    1087     681     598     467
                 406     354     330     327     309     255     219     191
                  60
 
SfoI GGC'GCC

Cuts at:   2083    3240    2083 
   Size:       1157    9242
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10399
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:      10399
 
SmaI CCC'GGG

Cuts at:   1872    6181    6347    7340    7726    1872 
   Size:       4309     166     993     386    4545

  Fragments arranged by size: 

                4545    4309     993     386     166
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4910    5629    6026    6147    6899    8503 
   Size:        197     926    2967     719     397     121     752    1604

Cuts at:   8503    9404    9666     820 
   Size:        901     262    1553

  Fragments arranged by size: 

                2967    1604    1553     926     901     752     719     397
                 262     197     121
 
SnaBI TAC'GTA

Cuts at:    493    8190     493 
   Size:       7697    2702
 
SpeI A'CTAG_T

Cuts at:    152    2357     152 
   Size:       2205    8194
 
SphI G_CATG'C

Cuts at:   1132    3277    5416    6727    7156    7555    7757    1132 
   Size:       2145    2139    1311     429     399     202    3774

  Fragments arranged by size: 

                3774    2145    2139    1311     429     399     202
 
SspI AAT'ATT

Cuts at:      5      52    8323       5 
   Size:         47    8271    2081

  Fragments arranged by size: 

                8271    2081      47
 
StuI AGG'CCT

Cuts at:   1517    6581    7100    1517 
   Size:       5064     519    4816

  Fragments arranged by size: 

                5064    4816     519
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2086    2608    2823    4383    4506    5423 
   Size:        552     939      82     522     215    1560     123     917

Cuts at:   5423    5574    5670    6267    6526    6606    6891    6924    7452 
   Size:        151      96     597     259      80     285      33     528

Cuts at:   7452    7617    7838     513 
   Size:        165     221    3074

  Fragments arranged by size: 

                3074    1560     939     917     597     552     528     522
                 285     259     221     215     165     151     123      96
                  82      80      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2333    2762    2794    3938    4042    4515    4519 
   Size:       1255     753     429      32    1144     104     473       4

Cuts at:   4519    4667    5755    5979    5983    8426    8443    8596    8781 
   Size:        148    1088     224       4    2443      17     153     185

Cuts at:   8781    9226     325 
   Size:        445    1498

  Fragments arranged by size: 

                2443    1498    1255    1144    1088     753     473     445
                 429     224     185     153     148     104      32      17
                   4       4
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2324    3642    3927 
   Size:        274      80      33      51    1443     347    1318     285

Cuts at:   3927    4545    5118    5193    6363    7230    7263    7290    8645 
   Size:        618     573      75    1170     867      33      27    1355

Cuts at:   8645   10357      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1355    1318    1170     867     618     573
                 347     285     274     138      80      75      51      33
                  33      27
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5234    5266    5747    5779    5837    5869   10359   10391    5234 
   Size:         32     481      32      58      32    4490      32    5242

  Fragments arranged by size: 

                5242    4490     481      58      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    4762    1316 
   Size:       3446    6953
 
XbaI T'CTAG_A

Cuts at:   5464    7735    5464 
   Size:       2271    8128
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2911    3292    4012    5398    5983    7039    2911 
   Size:        381     720    1386     585    1056    6271

  Fragments arranged by size: 

                6271    1386    1056     720     585     381
 
XhoI C'TCGA_G

Cuts at:   1943    6147    6899    1943 
   Size:       4204     752    5443

  Fragments arranged by size: 

                5443    4204     752
 
XmaI C'CCGG_G

Cuts at:   1870    6179    6345    7338    7724    1870 
   Size:       4309     166     993     386    4545

  Fragments arranged by size: 

                4545    4309     993     386     166
 
XmnI GAAnn'nnTTC

Cuts at:   8528    8528 
   Size:      10399
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4297    5055    8207     276 
   Size:         53      83     186     936    2763     758    3152    2468

  Fragments arranged by size: 

                3152    2763    2468     936     758     186      83      53
            
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AscI    AvrII     BaeI    BfrBI     BplI    BsmBI  BstZ17I    EcoRV
     NruI     NsiI     PacI     PsrI     SfiI     SrfI     SwaI
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