Restriction map for
pFN21AB1440
(Linear) (Six-base) MAPSORT of: KIBB1440.seq Check: 5694 from: 1 to: 5694
KIBB1440.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5424 5694
Size: 5424 270
AatII G_ACGT'C
Cuts at: 0 2294 3052 5694
Size: 2294 758 2642
Fragments arranged by size:
2642 2294 758
AccI GT'mk_AC
Cuts at: 0 524 1346 2180 2761 5567 5694
Size: 524 822 834 581 2806 127
Fragments arranged by size:
2806 834 822 581 524 127
Acc65I G'GTAC_C
Cuts at: 0 3381 5694
Size: 3381 2313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1735 3541 3898 5694
Size: 448 1287 1806 357 1796
Fragments arranged by size:
1806 1796 1287 448 357
AfeI AGC'GCT
Cuts at: 0 229 3679 5242 5694
Size: 229 3450 1563 452
Fragments arranged by size:
3450 1563 452 229
AflIII A'CryG_T
Cuts at: 0 643 4763 4928 5570 5694
Size: 643 4120 165 642 124
Fragments arranged by size:
4120 643 642 165 124
AleI CACnn'nnGTG
Cuts at: 0 892 1420 1477 2065 2170 2242 2830 4463
Size: 892 528 57 588 105 72 588 1633
Cuts at: 4463 4813 5489 5512 5694
Size: 350 676 23 182
Fragments arranged by size:
1633 892 676 588 588 528 350 182
105 72 57 23
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1450 2110 3029 5507 5694
Size: 1450 660 919 2478 187
Fragments arranged by size:
2478 1450 919 660 187
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4882 4914 5694
Size: 4882 32 780
Fragments arranged by size:
4882 780 32
AlwNI CAG_nnn'CTG
Cuts at: 0 490 658 2862 3240 4851 5694
Size: 490 168 2204 378 1611 843
Fragments arranged by size:
2204 1611 843 490 378 168
ApaI G_GGCC'C
Cuts at: 0 1084 2074 4249 4601 5694
Size: 1084 990 2175 352 1093
Fragments arranged by size:
2175 1093 1084 990 352
ApaLI G'TGCA_C
Cuts at: 0 770 4609 5482 5694
Size: 770 3839 873 212
Fragments arranged by size:
3839 873 770 212
ApoI r'AATT_y
Cuts at: 0 4077 5694
Size: 4077 1617
AvaI C'yCGr_G
Cuts at: 0 1309 2080 2704 3433 4142 4174 4255 4340
Size: 1309 771 624 729 709 32 81 85
Cuts at: 4340 4894 5333 5694
Size: 554 439 361
Fragments arranged by size:
1309 771 729 709 624 554 439 361
85 81 32
BamHI G'GATC_C
Cuts at: 0 275 1580 5694
Size: 275 1305 4114
Fragments arranged by size:
4114 1305 275
BanI G'GyrC_C
Cuts at: 0 33 76 568 1233 2389 2492 3381 4375
Size: 33 43 492 665 1156 103 889 994
Cuts at: 4375 4815 5694
Size: 440 879
Fragments arranged by size:
1156 994 889 879 665 492 440 103
43 33
BanII G_rGCy'C
Cuts at: 0 815 860 1084 1319 1412 1858 2074 2267
Size: 815 45 224 235 93 446 216 193
Cuts at: 2267 4249 4601 4895 5047 5694
Size: 1982 352 294 152 647
Fragments arranged by size:
1982 815 647 446 352 294 235 224
216 193 152 93 45
BbeI G_GCGC'C
Cuts at: 0 80 1237 5694
Size: 80 1157 4457
Fragments arranged by size:
4457 1157 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 1664 1767 3052 4693 5593 5694
Size: 93 1571 103 1285 1641 900 101
Fragments arranged by size:
1641 1571 1285 900 103 101 93
BbvCI CC'TCA_GC
Cuts at: 0 422 1135 5694
Size: 422 713 4559
Fragments arranged by size:
4559 713 422
BciVI GTATCCnnnnn_n'
Cuts at: 0 1330 5694
Size: 1330 4364
BclI T'GATC_A
Cuts at: 0 2453 4353 5694
Size: 2453 1900 1341
Fragments arranged by size:
2453 1900 1341
BglI GCCn_nnn'nGGC
Cuts at: 0 1907 1939 2127 2557 4637 5694
Size: 1907 32 188 430 2080 1057
Fragments arranged by size:
2080 1907 1057 430 188 32
BlpI GC'TnA_GC
Cuts at: 0 859 1273 3061 5694
Size: 859 414 1788 2633
Fragments arranged by size:
2633 1788 859 414
Bme1580I G_kGCm'C
Cuts at: 0 38 774 1075 1084 1218 2058 2074 2123
Size: 38 736 301 9 134 840 16 49
Cuts at: 2123 2394 3101 3146 4249 4601 4613 5399 5486
Size: 271 707 45 1103 352 12 786 87
Cuts at: 5486 5694
Size: 208
Fragments arranged by size:
1103 840 786 736 707 352 301 271
208 134 87 49 45 38 16 12
9
BmgBI CAC'GTC
Cuts at: 0 2067 2118 2759 4931 5694
Size: 2067 51 641 2172 763
Fragments arranged by size:
2172 2067 763 641 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 137 1650 1800 1914 5292 5694
Size: 137 1513 150 114 3378 402
Fragments arranged by size:
3378 1513 402 150 137 114
BmtI G_CTAG'C
Cuts at: 0 2302 5694
Size: 2302 3392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1224 1983 2041 2272 2470 3463 3672
Size: 874 350 759 58 231 198 993 209
Cuts at: 3672 4108 4296 4348 4455 4807 4850 5560 5680
Size: 436 188 52 107 352 43 710 120
Cuts at: 5680 5694
Size: 14
Fragments arranged by size:
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209 198 188 120 107 58 52 43
14
Bpu10I CC'TnA_GC
Cuts at: 0 8 422 1135 5694
Size: 8 414 713 4559
Fragments arranged by size:
4559 713 414 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2890 3645 4006 5694
Size: 2890 755 361 1688
Fragments arranged by size:
2890 1688 755 361
BsaI GGTCTCn'nnnn_
Cuts at: 0 2335 3947 4252 4375 5694
Size: 2335 1612 305 123 1319
Fragments arranged by size:
2335 1612 1319 305 123
BsaAI yAC'GTr
Cuts at: 0 786 2244 4070 4710 4764 4799 5694
Size: 786 1458 1826 640 54 35 895
Fragments arranged by size:
1826 1458 895 786 640 54 35
BsaHI Gr'CG_yC
Cuts at: 0 77 1234 1772 2291 3049 3415 4169 5694
Size: 77 1157 538 519 758 366 754 1525
Fragments arranged by size:
1525 1157 758 754 538 519 366 77
BsaWI w'CCGG_w
Cuts at: 0 1356 3618 5694
Size: 1356 2262 2076
Fragments arranged by size:
2262 2076 1356
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1675 1705 2995 3025 3742 3772 3828 3858
Size: 1675 30 1290 30 717 30 56 30
Cuts at: 3858 4027 4057 4321 4351 4744 4774 4882 4912
Size: 169 30 264 30 393 30 108 30
Cuts at: 4912 5694
Size: 782
Fragments arranged by size:
1675 1290 782 717 393 264 169 108
56 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 683 823 894 1327 1402 1683 2692 3499
Size: 683 140 71 433 75 281 1009 807
Cuts at: 3499 3757 3891 4345 5694
Size: 258 134 454 1349
Fragments arranged by size:
1349 1009 807 683 454 433 281 258
140 134 75 71
BseYI C'CCAG_C
Cuts at: 0 224 712 909 1198 1495 1538 2003 2122
Size: 224 488 197 289 297 43 465 119
Cuts at: 2122 2200 2523 2716 2974 3065 3731 4851 5694
Size: 78 323 193 258 91 666 1120 843
Fragments arranged by size:
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258 224 197 193 119 91 78 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 313 1279 1315 1733 2447 2602 4765
Size: 32 281 966 36 418 714 155 2163
Cuts at: 4765 5638 5694
Size: 873 56
Fragments arranged by size:
2163 966 873 714 418 281 155 56
36 32
BsiEI CG_ry'CG
Cuts at: 0 546 1421 3315 3872 5694
Size: 546 875 1894 557 1822
Fragments arranged by size:
1894 1822 875 557 546
BsiHKAI G_wGCw'C
Cuts at: 0 374 774 815 860 1997 3430 3554 4613
Size: 374 400 41 45 1137 1433 124 1059
Cuts at: 4613 4895 5131 5162 5486 5694
Size: 282 236 31 324 208
Fragments arranged by size:
1433 1137 1059 400 374 324 282 236
208 124 45 41 31
BsiWI C'GTAC_G
Cuts at: 0 870 5694
Size: 870 4824
BsmI GAATG_Cn'
Cuts at: 0 4230 5694
Size: 4230 1464
Bsp1286I G_dGCh'C
Cuts at: 0 38 374 774 815 860 1075 1084 1218
Size: 38 336 400 41 45 215 9 134
Cuts at: 1218 1319 1412 1858 1997 2058 2074 2123 2267
Size: 101 93 446 139 61 16 49 144
Cuts at: 2267 2394 3101 3146 3430 3554 4249 4601 4613
Size: 127 707 45 284 124 695 352 12
Cuts at: 4613 4895 5047 5131 5162 5399 5486 5694
Size: 282 152 84 31 237 87 208
Fragments arranged by size:
707 695 446 400 352 336 284 282
237 215 208 152 144 139 134 127
124 101 93 87 84 61 49 45
45 41 38 31 16 12 9
BspEI T'CCGG_A
Cuts at: 0 1356 3618 5694
Size: 1356 2262 2076
Fragments arranged by size:
2262 2076 1356
BspHI T'CATG_A
Cuts at: 0 4313 5694
Size: 4313 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2720 2744 3179 4723 4961 5424 5694
Size: 2720 24 435 1544 238 463 270
Fragments arranged by size:
2720 1544 463 435 270 238 24
BsrBI CCG'CTC
Cuts at: 0 1614 4671 5694
Size: 1614 3057 1023
Fragments arranged by size:
3057 1614 1023
BsrDI GCAATG_nn'
Cuts at: 0 283 870 3047 5694
Size: 283 587 2177 2647
Fragments arranged by size:
2647 2177 587 283
BsrFI r'CCGG_y
Cuts at: 0 2438 2531 2611 2997 3605 4636 4976 5694
Size: 2438 93 80 386 608 1031 340 718
Fragments arranged by size:
2438 1031 718 608 386 340 93 80
BsrGI T'GTAC_A
Cuts at: 0 319 3113 5694
Size: 319 2794 2581
Fragments arranged by size:
2794 2581 319
BssSI C'ACGA_G
Cuts at: 0 534 1590 4681 5694
Size: 534 1056 3091 1013
Fragments arranged by size:
3091 1056 1013 534
BstAPI GCAn_nnn'nTGC
Cuts at: 0 769 1530 3150 4640 5394 5694
Size: 769 761 1620 1490 754 300
Fragments arranged by size:
1620 1490 769 761 754 300
BstEII G'GTnAC_C
Cuts at: 0 1674 2235 4401 5694
Size: 1674 561 2166 1293
Fragments arranged by size:
2166 1674 1293 561
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 1326 2772 3000 4465 5070 5694
Size: 414 912 1446 228 1465 605 624
Fragments arranged by size:
1465 1446 912 624 605 414 228
BstYI r'GATC_y
Cuts at: 0 275 1580 2219 5694
Size: 275 1305 639 3475
Fragments arranged by size:
3475 1305 639 275
Bsu36I CC'TnA_GG
Cuts at: 0 116 1607 5694
Size: 116 1491 4087
Fragments arranged by size:
4087 1491 116
BtgI C'CryG_G
Cuts at: 0 81 1421 1478 1544 1862 1928 1959 2134
Size: 81 1340 57 66 318 66 31 175
Cuts at: 2134 2223 2268 2462 2828 3210 3665 4262 4371
Size: 89 45 194 366 382 455 597 109
Cuts at: 4371 4521 4601 4886 5031 5612 5694
Size: 150 80 285 145 581 82
Fragments arranged by size:
1340 597 581 455 382 366 318 285
194 175 150 145 109 89 82 81
80 66 66 57 45 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1499 3792 4110 4218 5694
Size: 1499 2293 318 108 1476
Fragments arranged by size:
2293 1499 1476 318 108
BtsI GCAGTG_nn'
Cuts at: 0 594 770 1214 1303 2165 2474 4611 5484
Size: 594 176 444 89 862 309 2137 873
Cuts at: 5484 5496 5694
Size: 12 198
Fragments arranged by size:
2137 873 862 594 444 309 198 176
89 12
ClaI AT'CG_AT
Cuts at: 0 4163 4205 5694
Size: 4163 42 1489
Fragments arranged by size:
4163 1489 42
DraIII CAC_nnn'GTG
Cuts at: 0 1674 3243 5694
Size: 1674 1569 2451
Fragments arranged by size:
2451 1674 1569
DrdI GACnn_nn'nnGTC
Cuts at: 0 132 2932 5694
Size: 132 2800 2762
Fragments arranged by size:
2800 2762 132
EaeI y'GGCC_r
Cuts at: 0 543 840 896 1106 2214 2465 3312 4301
Size: 543 297 56 210 1108 251 847 989
Cuts at: 4301 4363 4427 4513 4704 4803 4910 5230 5380
Size: 62 64 86 191 99 107 320 150
Cuts at: 5380 5694
Size: 314
Fragments arranged by size:
1108 989 847 543 320 314 297 251
210 191 150 107 99 86 64 62
56
EagI C'GGCC_G
Cuts at: 0 543 3312 5694
Size: 543 2769 2382
Fragments arranged by size:
2769 2382 543
EarI CTCTTCn'nnn_
Cuts at: 0 371 956 5694
Size: 371 585 4738
Fragments arranged by size:
4738 585 371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 491 1809 2812 3494 4000 5694
Size: 491 1318 1003 682 506 1694
Fragments arranged by size:
1694 1318 1003 682 506 491
EcoICRI GAG'CTC
Cuts at: 0 813 858 4893 5694
Size: 813 45 4035 801
Fragments arranged by size:
4035 813 801 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 874 1224 1735 1983 2041 2272 2470
Size: 448 426 350 511 248 58 231 198
Cuts at: 2470 3463 3541 3672 3898 4108 4296 4348 4455
Size: 993 78 131 226 210 188 52 107
Cuts at: 4455 4807 4850 5560 5680 5694
Size: 352 43 710 120 14
Fragments arranged by size:
993 710 511 448 426 352 350 248
231 226 210 198 188 131 120 107
78 58 52 43 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2620 4396 5694
Size: 2620 1776 1298
Fragments arranged by size:
2620 1776 1298
EcoO109I rG'GnC_Cy
Cuts at: 0 418 1080 1081 1710 1881 2007 2071 2699
Size: 418 662 1 629 171 126 64 628
Cuts at: 2699 2709 2781 3228 3769 3836 4245 4246 4597
Size: 10 72 447 541 67 409 1 351
Cuts at: 4597 5113 5694
Size: 516 581
Fragments arranged by size:
662 629 628 581 541 516 447 418
409 351 171 126 72 67 64 10
1 1
EcoRI G'AATT_C
Cuts at: 0 4077 5694
Size: 4077 1617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1695 3903 5694
Size: 1695 2208 1791
Fragments arranged by size:
2208 1791 1695
FseI GG_CCGG'CC
Cuts at: 0 2442 5694
Size: 2442 3252
FspI TGC'GCA
Cuts at: 0 2104 4444 5694
Size: 2104 2340 1250
Fragments arranged by size:
2340 2104 1250
FspAI rTGC'GCAy
Cuts at: 0 2104 4444 5694
Size: 2104 2340 1250
Fragments arranged by size:
2340 2104 1250
HaeII r_GCGC'y
Cuts at: 0 80 231 1237 1280 3681 4002 4783 4949
Size: 80 151 1006 43 2401 321 781 166
Cuts at: 4949 5244 5694
Size: 295 450
Fragments arranged by size:
2401 1006 781 450 321 295 166 151
80 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1328 1360 1552 1584 1675 1707 2264 2296
Size: 1328 32 192 32 91 32 557 32
Cuts at: 2296 3130 3162 3828 3850 3860 3882 4027 4059
Size: 834 32 666 22 10 22 145 32
Cuts at: 4059 4321 4353 5329 5361 5694
Size: 262 32 976 32 333
Fragments arranged by size:
1328 976 834 666 557 333 262 192
145 91 32 32 32 32 32 32
32 32 22 22 10
HincII GTy'rAC
Cuts at: 0 5271 5568 5694
Size: 5271 297 126
Fragments arranged by size:
5271 297 126
HindIII A'AGCT_T
Cuts at: 0 5191 5694
Size: 5191 503
KasI G'GCGC_C
Cuts at: 0 76 1233 5694
Size: 76 1157 4461
Fragments arranged by size:
4461 1157 76
KpnI G_GTAC'C
Cuts at: 0 3385 5694
Size: 3385 2309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1550 4391 4646 5694
Size: 623 927 2841 255 1048
Fragments arranged by size:
2841 1048 927 623 255
MscI TGG'CCA
Cuts at: 0 898 1108 2216 4365 4429 4515 4706 4912
Size: 898 210 1108 2149 64 86 191 206
Cuts at: 4912 5232 5382 5694
Size: 320 150 312
Fragments arranged by size:
2149 1108 898 320 312 210 206 191
150 86 64
MslI CAynn'nnrTG
Cuts at: 0 196 412 892 1291 1420 1477 2065 2170
Size: 196 216 480 399 129 57 588 105
Cuts at: 2170 2242 2449 2467 2830 2998 3376 4463 4606
Size: 72 207 18 363 168 378 1087 143
Cuts at: 4606 4813 5179 5489 5512 5694
Size: 207 366 310 23 182
Fragments arranged by size:
1087 588 480 399 378 366 363 310
216 207 207 196 182 168 143 129
105 72 57 23 18
MspA1I CmG'CkG
Cuts at: 0 387 426 558 1069 1546 1743 1961 2003
Size: 387 39 132 511 477 197 218 42
Cuts at: 2003 2126 2136 2225 2327 2720 3065 3094 3285
Size: 123 10 89 102 393 345 29 191
Cuts at: 3285 3492 5640 5694
Size: 207 2148 54
Fragments arranged by size:
2148 511 477 393 387 345 218 207
197 191 132 123 102 89 54 42
39 29 10
NaeI GCC'GGC
Cuts at: 0 2440 5694
Size: 2440 3254
NarI GG'CG_CC
Cuts at: 0 77 1234 5694
Size: 77 1157 4460
Fragments arranged by size:
4460 1157 77
NcoI C'CATG_G
Cuts at: 0 81 3665 4262 4521 4601 4886 5612 5694
Size: 81 3584 597 259 80 285 726 82
Fragments arranged by size:
3584 726 597 285 259 82 81 80
NdeI CA'TA_TG
Cuts at: 0 3528 5694
Size: 3528 2166
NgoMIV G'CCGG_C
Cuts at: 0 2438 5694
Size: 2438 3256
NheI G'CTAG_C
Cuts at: 0 2298 5694
Size: 2298 3396
NspI r_CATG'y
Cuts at: 0 758 1272 1972 2179 2743 3411 4722 5151
Size: 758 514 700 207 564 668 1311 429
Cuts at: 5151 5295 5550 5574 5694
Size: 144 255 24 120
Fragments arranged by size:
1311 758 700 668 564 514 429 255
207 144 120 24
PasI CC'CwG_GG
Cuts at: 0 19 394 5694
Size: 19 375 5300
Fragments arranged by size:
5300 375 19
PciI A'CATG_T
Cuts at: 0 5570 5694
Size: 5570 124
PflMI CCAn_nnn'nTGG
Cuts at: 0 4372 4522 4851 5694
Size: 4372 150 329 843
Fragments arranged by size:
4372 843 329 150
PfoI T'CCnGG_A
Cuts at: 0 851 2986 3554 5069 5537 5694
Size: 851 2135 568 1515 468 157
Fragments arranged by size:
2135 1515 851 568 468 157
PmlI CAC'GTG
Cuts at: 0 4070 4710 4799 5694
Size: 4070 640 89 895
Fragments arranged by size:
4070 895 640 89
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4882 4914 5694
Size: 4882 32 780
Fragments arranged by size:
4882 780 32
PpuMI rG'GwC_Cy
Cuts at: 0 1710 1881 2007 2699 2709 2781 3769 3836
Size: 1710 171 126 692 10 72 988 67
Cuts at: 3836 5694
Size: 1858
Fragments arranged by size:
1858 1710 988 692 171 126 72 67
10
PspOMI G'GGCC_C
Cuts at: 0 1080 2070 4245 4597 5694
Size: 1080 990 2175 352 1097
Fragments arranged by size:
2175 1097 1080 990 352
PspXI vC'TCGA_Gb
Cuts at: 0 4894 5694
Size: 4894 800
PstI C_TGCA'G
Cuts at: 0 2540 2867 3176 3236 4618 5024 5491 5694
Size: 2540 327 309 60 1382 406 467 203
Fragments arranged by size:
2540 1382 467 406 327 309 203 60
PvuI CG_AT'CG
Cuts at: 0 3872 5694
Size: 3872 1822
PvuII CAG'CTG
Cuts at: 0 387 426 558 1743 2003 3065 3094 5640
Size: 387 39 132 1185 260 1062 29 2546
Cuts at: 5640 5694
Size: 54
Fragments arranged by size:
2546 1185 1062 387 260 132 54 39
29
RsrII CG'GwC_CG
Cuts at: 0 1866 5694
Size: 1866 3828
SacI G_AGCT'C
Cuts at: 0 815 860 4895 5694
Size: 815 45 4035 799
Fragments arranged by size:
4035 815 799 45
SacII CC_GC'GG
Cuts at: 0 1547 1962 2137 2226 5694
Size: 1547 415 175 89 3468
Fragments arranged by size:
3468 1547 415 175 89
SalI G'TCGA_C
Cuts at: 0 5566 5694
Size: 5566 128
SanDI GG'GwC_CC
Cuts at: 0 1881 2781 3769 5694
Size: 1881 900 988 1925
Fragments arranged by size:
1925 1881 988 900
SapI GCTCTTCn'nnn_
Cuts at: 0 956 5694
Size: 956 4738
ScaI AGT'ACT
Cuts at: 0 1924 2542 3190 4360 5227 5260 5694
Size: 1924 618 648 1170 867 33 434
Fragments arranged by size:
1924 1170 867 648 618 434 33
SexAI A'CCwGG_T
Cuts at: 0 2009 2231 3239 5694
Size: 2009 222 1008 2455
Fragments arranged by size:
2455 2009 1008 222
SfcI C'TryA_G
Cuts at: 0 167 521 1119 1449 2536 2863 3172 3232
Size: 167 354 598 330 1087 327 309 60
Cuts at: 3232 4614 5020 5487 5694
Size: 1382 406 467 207
Fragments arranged by size:
1382 1087 598 467 406 354 330 327
309 207 167 60
SfoI GGC'GCC
Cuts at: 0 78 1235 5694
Size: 78 1157 4459
Fragments arranged by size:
4459 1157 78
SmaI CCC'GGG
Cuts at: 0 4176 4342 5335 5694
Size: 4176 166 993 359
Fragments arranged by size:
4176 993 359 166
SmlI C'TyrA_G
Cuts at: 0 2905 3624 4021 4142 4894 5694
Size: 2905 719 397 121 752 800
Fragments arranged by size:
2905 800 752 719 397 121
SpeI A'CTAG_T
Cuts at: 0 352 5694
Size: 352 5342
SphI G_CATG'C
Cuts at: 0 1272 3411 4722 5151 5550 5694
Size: 1272 2139 1311 429 399 144
Fragments arranged by size:
2139 1311 1272 429 399 144
StuI AGG'CCT
Cuts at: 0 4576 5095 5694
Size: 4576 519 599
Fragments arranged by size:
4576 599 519
StyI C'CwwG_G
Cuts at: 0 81 603 818 2378 2501 3418 3569 3665
Size: 81 522 215 1560 123 917 151 96
Cuts at: 3665 4262 4521 4601 4886 4919 5447 5612 5694
Size: 597 259 80 285 33 528 165 82
Fragments arranged by size:
1560 917 597 528 522 285 259 215
165 151 123 96 82 81 80 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 328 757 789 1933 2037 2510 2514 2662
Size: 328 429 32 1144 104 473 4 148
Cuts at: 2662 3750 3974 3978 5694
Size: 1088 224 4 1716
Fragments arranged by size:
1716 1144 1088 473 429 328 224 148
104 32 4 4
TatI w'GTAC_w
Cuts at: 0 319 1637 1922 2540 3113 3188 4358 5225
Size: 319 1318 285 618 573 75 1170 867
Cuts at: 5225 5258 5285 5694
Size: 33 27 409
Fragments arranged by size:
1318 1170 867 618 573 409 319 285
75 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3229 3261 3742 3774 3832 3864 5694
Size: 3229 32 481 32 58 32 1830
Fragments arranged by size:
3229 1830 481 58 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2757 5694
Size: 2757 2937
XbaI T'CTAG_A
Cuts at: 0 3459 5694
Size: 3459 2235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 906 1287 2007 3393 3978 5034 5694
Size: 906 381 720 1386 585 1056 660
Fragments arranged by size:
1386 1056 906 720 660 585 381
XhoI C'TCGA_G
Cuts at: 0 4142 4894 5694
Size: 4142 752 800
Fragments arranged by size:
4142 800 752
XmaI C'CCGG_G
Cuts at: 0 4174 4340 5333 5694
Size: 4174 166 993 361
Fragments arranged by size:
4174 993 361 166
ZraI GAC'GTC
Cuts at: 0 2292 3050 5694
Size: 2292 758 2644
Fragments arranged by size:
2644 2292 758
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfcI SfoI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AscI AseI AsiSI AvrII
BaeI BcgI BfrBI BglII BplI BsaBI BsmBI BssHII
BstBI BstZ17I CspCI DraI EcoRV HpaI MfeI MluI
NotI NruI NsiI PacI PmeI PshAI PsiI PsrI
SbfI SfiI SgfI SgrAI SnaBI SrfI SspI SwaI
XmnI