Restriction map for
pFN21AB1380
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cac8I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FbaI FseI
FspI HapII HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MluI MluNI MroI MscI
MspI NaeI NarI NciI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 1342 CGCG CG^CG
AciI 794 GCGG CCGC(-3/-1)
AcyI 1847 GACGCC G[GA]^CG[CT]C
AfaI 1900 GTAC GT^AC
Alw44I 1765 GTGCAC G^TGCAC
AlwNI 1675 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 177 CCTAAGG CC^T[ACGT]AGG
ApaI 1400 GGGCCC GGGCC^C
ApaLI 1765 GTGCAC G^TGCAC
AspHI 1765 GTGCAC G[AT]GC[AT]^C
BanII 1400 GGGCCC G[GA]GC[CT]^C
BciVI 162 GGATAC GTATCC(6/5)
BglII 1071 AGATCT A^GATCT
BsaHI 1847 GACGCC G[GA]^CG[CT]C
BseRI 1039 GAGGAG GAGGAG(10/8)
BsgI 1681 CTGCAC GTGCAG(16/14)
BsiHKAI 1765 GTGCAC G[AT]GC[AT]^C
BsmBI 1845 GAGACG CGTCTC(1/5)
BsmFI 141 GGGAC GGGAC(10/14)
Bsp1407I 1899 TGTACA T^GTACA
BspMI 128 ACCTGC ACCTGC(4/8)
BsrGI 1899 TGTACA T^GTACA
BstUI 1342 CGCG CG^CG
BstXI 444 CCAGGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 177 CCTAAGG CC^T[ACGT]AGG
DraII 1400 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 920 CCATGG C^C[GA][CT]GG
Eco81I 177 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1400 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 920 CCATGG C^C[AT][AT]GG
EcoT22I 2005 ATGCAT ATGCA^T
FauI 794 GCGGG CCCGC(4/6)
HaeII 1988 GGCGCT [GA]GCGC^[CT]
Hin1I 1847 GACGCC G[GA]^CG[CT]C
MfeI 1620 CAATTG C^AATTG
MspA1I 1815 CAGCTG C[AC]G^C[GT]G
MunI 1620 CAATTG C^AATTG
MvnI 1342 CGCG CG^CG
MwoI 63 GCTGGGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 920 CCATGG C^CATGG
NdeI 1087 CATATG CA^TATG
NsiI 2005 ATGCAT ATGCA^T
PvuII 1815 CAGCTG CAG^CTG
RsaI 1900 GTAC GT^AC
SexAI 334 ACCAGGT A^CC[AT]GGT
SspBI 1899 TGTACA T^GTACA
StyI 920 CCATGG C^C[AT][AT]GG
Tsp45I 617 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
BbsI 204 GAAGAC GAAGAC(2/6)
46 GTCTTC
BbvI 1881 GCAGC GCAGC(8/12)
69 GCTGC
BmyI 1400 GGGCCC G[AGT]GC[ACT]^C
1765 GTGCAC
BpuAI 204 GAAGAC GAAGAC(2/6)
46 GTCTTC
BsaAI 528 TACGTG [CT]AC^GT[GA]
957 TACGTG
BsaBI 490 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2002 GATATGCATC
BsaI 1611 GAGACC GGTCTC(1/5)
338 GGTCTC
BsiYI 396 CCAGACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
843 CCATACGGGGG
BslI 396 CCAGACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
843 CCATACGGGGG
Bsp1286I 1400 GGGCCC G[AGT]GC[ACT]^C
1765 GTGCAC
BsrDI 19 GCAATG GCAATG(2/0)
449 GCAATG
BssKI 335 CCAGG ^CC[ACGT]GG
444 CCAGG
BssSI 1152 CTCGTG CACGAG(-5/-1)
1656 CTCGTG
BstF5I 464 CATCC GGATG(2/0)
197 GGATG
BstNI 335 CCAGG CC^[AT]GG
444 CCAGG
CfoI 1341 GCGC GCG^C
1989 GCGC
EcoRII 335 CCAGG ^CC[AT]GG
444 CCAGG
Fnu4HI 69 GCTGC GC^[ACGT]GC
1881 GCAGC
FokI 464 CATCC GGATG(9/13)
197 GGATG
HgaI 1847 GACGC GACGC(5/10)
1343 GCGTC
HhaI 1341 GCGC GCG^C
1989 GCGC
HinP1I 1341 GCGC G^CGC
1989 GCGC
HphI 382 GGTGA GGTGA(8/7)
1335 TCACC
ItaI 69 GCTGC GC^[ACGT]GC
1881 GCAGC
MaeIII 557 GTTAC ^GT[ACGT]AC
617 GTGAC
MamI 490 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2002 GATATGCATC
MvaI 335 CCAGG CC^[AT]GG
444 CCAGG
ScrFI 335 CCAGG CC^[ACGT]GG
444 CCAGG
SmlI 341 CTCAAG C^T[CT][GA]AG
2076 CTCAAG
StuI 800 AGGCCT AGG^CCT
2072 AGGCCT
TaqI 99 TCGA T^CGA
2085 TCGA
TseI 69 GCTGC G^C[AT]GC
1881 GCAGC
TthHB8I 99 TCGA T^CGA
2085 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AflIII AluI AlwI ApoI
Asp700I AvaII BpmI BsaJI BsmAI
BsmI BsrI BstYI Cfr13I DdeI
DpnII EarI EcoRI HaeIII HinfI
Ksp632I MaeII MboI MboII MflI
MnlI MseI MslI NdeII NlaIII
NlaIV PleI SapI Sau3AI Sau96I
SfaNI TfiI Tru9I Tsp509I TspRI
XhoII XmnI