(Circular) (Six-base) MAPSORT of: pFN21AB1380.seq Check: 5886 from: 1 to: 6826 pFN21AB1380 6826 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4636 278 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4169 1937 Size: 2232 4594 Acc65I G'GTAC_C Cuts at: 4147 4147 Size: 6826 AclI AA'CG_TT Cuts at: 1563 4953 5326 1563 Size: 3390 373 3063 Fragments arranged by size: 3390 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2110 2458 2710 3130 3802 4889 6273 6541 Size: 478 348 252 420 672 1087 1384 268 Cuts at: 6541 1632 Size: 1917 Fragments arranged by size: 1917 1384 1087 672 478 420 348 268 252 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6629 AflIII A'CryG_T Cuts at: 1203 2534 2625 2963 5725 6702 1203 Size: 1331 91 338 2762 977 1327 Fragments arranged by size: 2762 1331 1327 977 338 91 AgeI A'CCGG_T Cuts at: 5657 5657 Size: 6826 AhdI GACnn_n'nnGTC Cuts at: 5555 5555 Size: 6826 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6826 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6826 AlwNI CAG_nnn'CTG Cuts at: 3685 4569 6141 3685 Size: 884 1572 4370 Fragments arranged by size: 4370 1572 884 ApaI G_GGCC'C Cuts at: 3409 3409 Size: 6826 ApaLI G'TGCA_C Cuts at: 3770 4883 6039 3770 Size: 1113 1156 4557 Fragments arranged by size: 4557 1156 1113 ApoI r'AATT_y Cuts at: 1493 2233 2639 3238 3490 3742 3910 4135 4361 Size: 740 406 599 252 252 168 225 226 Cuts at: 4361 4513 6622 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 740 599 406 252 252 226 225 168 152 AseI AT'TA_AT Cuts at: 160 1032 5380 160 Size: 872 4348 1606 Fragments arranged by size: 4348 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 AvaI C'yCGr_G Cuts at: 1870 1943 4151 1870 Size: 73 2208 4545 Fragments arranged by size: 4545 2208 73 BamHI G'GATC_C Cuts at: 4156 4523 4156 Size: 367 6459 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4147 5602 618 Size: 325 217 627 2360 1455 1842 Fragments arranged by size: 2360 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3409 4145 1391 Size: 2018 736 4072 Fragments arranged by size: 4072 2018 736 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 2216 928 Size: 971 145 172 5538 Fragments arranged by size: 5538 971 172 145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5017 5051 1903 Size: 34 3080 34 3678 Fragments arranged by size: 3678 3080 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2161 4718 5928 2161 Size: 2557 1210 3059 Fragments arranged by size: 3059 2557 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6826 BfrBI ATG'CAT Cuts at: 4012 4012 Size: 6826 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5437 136 Size: 107 122 71 631 605 3765 1525 Fragments arranged by size: 3765 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3076 6821 3076 Size: 3745 3081 BlpI GC'TnA_GC Cuts at: 4243 4243 Size: 6826 Bme1580I G_kGCm'C Cuts at: 1790 3409 3774 4887 6043 1790 Size: 1619 365 1113 1156 2573 Fragments arranged by size: 2573 1619 1156 1113 365 BmgBI CAC'GTC Cuts at: 1318 2041 2626 1318 Size: 723 585 5518 Fragments arranged by size: 5518 723 585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4557 5515 442 Size: 416 53 83 399 24 3140 958 1753 Fragments arranged by size: 3140 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6826 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3501 3501 Size: 6826 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2706 3546 3798 5486 1239 Size: 899 568 840 252 1688 2579 Fragments arranged by size: 2579 1688 899 840 568 252 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2331 4066 4951 5816 6114 2331 Size: 1735 885 865 298 3043 Fragments arranged by size: 3043 1735 885 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2349 3610 5489 882 Size: 617 850 1261 1879 2219 Fragments arranged by size: 2219 1879 1261 850 617 BsaAI yAC'GTr Cuts at: 493 1204 2535 2964 4617 493 Size: 711 1331 429 1653 2702 Fragments arranged by size: 2702 1653 1331 711 429 BsaBI GATnn'nnATC Cuts at: 2499 4011 6820 2499 Size: 1512 2809 2505 Fragments arranged by size: 2809 2505 1512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3853 4633 5015 5650 Size: 53 83 186 936 2320 780 382 635 Cuts at: 5650 275 Size: 1451 Fragments arranged by size: 2320 1451 936 780 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5259 5657 5931 6078 6489 5259 Size: 398 274 147 411 5596 Fragments arranged by size: 5596 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6601 Fragments arranged by size: 6601 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3059 1186 Size: 1873 4953 BseYI C'CCAG_C Cuts at: 2068 2823 3654 6029 6525 2068 Size: 755 831 2375 496 2369 Fragments arranged by size: 2375 2369 831 755 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3671 1163 Size: 573 1935 4318 Fragments arranged by size: 4318 1935 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4276 5037 6065 1540 Size: 275 186 2275 761 1028 2301 Fragments arranged by size: 2301 2275 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3774 4887 4972 6043 3774 Size: 1113 85 1071 4557 Fragments arranged by size: 4557 1113 1071 85 BsmI GAATG_Cn' Cuts at: 2156 2801 2920 4337 4430 2156 Size: 645 119 1417 93 4552 Fragments arranged by size: 4552 1417 645 119 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3844 3844 Size: 6826 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3409 3774 4145 4887 4972 6043 1391 Size: 399 1619 365 371 742 85 1071 2174 Fragments arranged by size: 2174 1619 1071 742 399 371 365 85 BspEI T'CCGG_A Cuts at: 6489 6489 Size: 6826 BspHI T'CATG_A Cuts at: 4715 6445 4715 Size: 1730 5096 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2142 4181 844 Size: 1298 2039 3489 Fragments arranged by size: 3489 2039 1298 BsrBI CCG'CTC Cuts at: 1394 1823 4713 1394 Size: 429 2890 3507 Fragments arranged by size: 3507 2890 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2031 2461 5321 5495 65 Size: 1183 783 430 2860 174 1396 Fragments arranged by size: 2860 1396 1183 783 430 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5470 5657 1700 Size: 110 3660 187 2869 Fragments arranged by size: 3660 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3904 96 Size: 3808 3018 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6826 BssSI C'ACGA_G Cuts at: 3157 3661 4886 5898 3157 Size: 504 1225 1012 4085 Fragments arranged by size: 4085 1225 1012 504 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6826 BstBI TT'CG_AA Cuts at: 5630 5630 Size: 6826 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6826 BstXI CCAn_nnnn'nTGG Cuts at: 2456 2456 Size: 6826 BstYI r'GATC_y Cuts at: 1972 2247 3076 3263 3328 4156 4523 4908 4925 Size: 275 829 187 65 828 367 385 17 Cuts at: 4925 6377 6463 6475 6821 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 829 828 385 367 346 275 187 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 2183 2183 Size: 6826 BtgI C'CryG_G Cuts at: 513 1065 2925 513 Size: 552 1860 4414 Fragments arranged by size: 4414 1860 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2990 487 Size: 683 56 1764 4323 Fragments arranged by size: 4323 1764 683 56 BtsI GCAGTG_nn' Cuts at: 4342 5136 5156 4342 Size: 794 20 6012 Fragments arranged by size: 6012 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6826 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6791 DraI TTT'AAA Cuts at: 4130 4130 Size: 6826 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5833 809 Size: 507 4517 1802 Fragments arranged by size: 4517 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4273 5162 6791 Size: 54 1425 50 275 18 2443 889 1629 Cuts at: 6791 8 Size: 43 Fragments arranged by size: 2443 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4273 1537 Size: 275 2461 4090 Fragments arranged by size: 4090 2461 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 3134 3386 3459 3470 3638 3711 4756 Size: 705 1076 252 73 11 168 73 1045 Cuts at: 4756 1353 Size: 3423 Fragments arranged by size: 3423 1076 1045 705 252 168 73 73 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5413 5787 5933 387 Size: 1064 138 3824 374 146 1280 Fragments arranged by size: 3824 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2138 2458 2706 2710 3130 3546 Size: 393 478 28 320 248 4 420 416 Cuts at: 3546 3798 3802 4889 5486 6273 6541 1239 Size: 252 4 1087 597 787 268 1524 Fragments arranged by size: 1524 1087 787 597 478 420 416 393 320 268 252 248 28 4 4 EcoO109I rG'GnC_Cy Cuts at: 3406 3406 Size: 6826 EcoRI G'AATT_C Cuts at: 2233 2639 3742 3910 4135 4513 2233 Size: 406 1103 168 225 378 4546 Fragments arranged by size: 4546 1103 406 378 225 168 FspI TGC'GCA Cuts at: 5332 5332 Size: 6826 HaeII r_GCGC'y Cuts at: 1678 3997 5973 1678 Size: 2319 1976 2531 Fragments arranged by size: 2531 2319 1976 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2232 2264 2641 2673 2965 Size: 32 135 32 660 32 377 32 292 Cuts at: 2965 2997 3313 3345 3385 3417 5467 5499 5541 Size: 32 316 32 40 32 2050 32 42 Cuts at: 5541 5573 1373 Size: 32 2626 Fragments arranged by size: 2626 2050 660 377 316 292 135 42 40 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4170 4416 669 Size: 988 281 2232 246 3079 Fragments arranged by size: 3079 2232 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6826 HpaI GTT'AAC Cuts at: 4416 4416 Size: 6826 KpnI G_GTAC'C Cuts at: 4151 4151 Size: 6826 MfeI C'AATT_G Cuts at: 3625 4425 3625 Size: 800 6026 MluI A'CGCG_T Cuts at: 6702 6702 Size: 6826 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3306 4093 5940 6124 1305 Size: 2001 787 1847 184 2007 Fragments arranged by size: 2007 2001 1847 787 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5004 Fragments arranged by size: 5004 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2535 2869 2953 3037 3205 3289 3457 Size: 648 1369 334 84 84 168 84 168 Cuts at: 3457 3541 3709 3793 3961 4045 4101 4784 5143 Size: 84 168 84 168 84 56 683 359 Cuts at: 5143 5302 518 Size: 159 2042 Fragments arranged by size: 2042 1369 683 648 359 334 168 168 168 168 159 84 84 84 84 84 84 56 MspA1I CmG'CkG Cuts at: 1606 3822 4242 4919 6067 6312 1606 Size: 2216 420 677 1148 245 2120 Fragments arranged by size: 2216 2120 1148 677 420 245 NcoI C'CATG_G Cuts at: 513 1065 2925 513 Size: 552 1860 4414 Fragments arranged by size: 4414 1860 552 NdeI CA'TA_TG Cuts at: 387 3093 387 Size: 2706 4120 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6826 NotI GC'GGCC_GC Cuts at: 4273 4273 Size: 6826 NsiI A_TGCA'T Cuts at: 4014 4014 Size: 6826 NspI r_CATG'y Cuts at: 1132 1229 4184 5729 1132 Size: 97 2955 1545 2229 Fragments arranged by size: 2955 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2449 1348 Size: 1101 5725 PciI A'CATG_T Cuts at: 5725 5725 Size: 6826 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6826 PmeI GTTT'AAAC Cuts at: 4130 4130 Size: 6826 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2123 2155 2681 2713 4895 4927 6408 6440 2123 Size: 32 526 32 2182 32 1481 32 2509 Fragments arranged by size: 2509 2182 1481 526 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6826 PsiI TTA'TAA Cuts at: 2784 4396 2784 Size: 1612 5214 PspOMI G'GGCC_C Cuts at: 3405 3405 Size: 6826 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2546 2578 2546 Size: 32 6794 PstI C_TGCA'G Cuts at: 830 4178 830 Size: 3348 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6826 PvuII CAG'CTG Cuts at: 3822 3822 Size: 6826 SalI G'TCGA_C Cuts at: 1936 4168 1936 Size: 2232 4594 SapI GCTCTTCn'nnn_ Cuts at: 2058 3134 3386 2058 Size: 1076 252 5498 Fragments arranged by size: 5498 1076 252 SbfI CC_TGCA'GG Cuts at: 4178 4178 Size: 6826 ScaI AGT'ACT Cuts at: 5074 6786 5074 Size: 1712 5114 SexAI A'CCwGG_T Cuts at: 2339 2339 Size: 6826 SfcI C'TryA_G Cuts at: 826 1045 4174 5309 5990 6181 826 Size: 219 3129 1135 681 191 1471 Fragments arranged by size: 3129 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6826 SmaI CCC'GGG Cuts at: 1872 4153 1872 Size: 2281 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2346 4081 4930 5831 6093 820 Size: 197 926 403 1735 849 901 262 1553 Fragments arranged by size: 1735 1553 926 901 849 403 262 197 SnaBI TAC'GTA Cuts at: 493 4617 493 Size: 4124 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6826 SphI G_CATG'C Cuts at: 1132 4184 1132 Size: 3052 3774 SspI AAT'ATT Cuts at: 5 52 4750 5 Size: 47 4698 2081 Fragments arranged by size: 4698 2081 47 StuI AGG'CCT Cuts at: 1517 2807 4079 1517 Size: 1290 1272 4264 Fragments arranged by size: 4264 1290 1272 StyI C'CwwG_G Cuts at: 513 1065 2925 4265 513 Size: 552 1860 1340 3074 Fragments arranged by size: 3074 1860 1340 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4853 4870 5023 5208 5653 325 Size: 1255 3273 17 153 185 445 1498 Fragments arranged by size: 3273 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3904 5072 6784 Size: 274 80 33 51 1443 1927 1168 1712 Cuts at: 6784 96 Size: 138 Fragments arranged by size: 1927 1712 1443 1168 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3248 3280 6786 6818 3248 Size: 32 3506 32 3256 Fragments arranged by size: 3506 3256 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6826 XbaI T'CTAG_A Cuts at: 4162 4162 Size: 6826 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6826 XmaI C'CCGG_G Cuts at: 1870 4151 1870 Size: 2281 4545 XmnI GAAnn'nnTTC Cuts at: 2696 2723 2807 2864 2948 3143 3815 3914 3936 Size: 27 84 57 84 195 672 99 22 Cuts at: 3936 4955 2696 Size: 1019 4567 Fragments arranged by size: 4567 1019 672 195 99 84 84 57 27 22 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4634 276 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI