Restriction map for
pFN21AB1380
(Linear) (Six-base) MAPSORT of: KIBB1380.seq Check: 1517 from: 1 to: 2121
KIBB1380.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 453 705 1125 1797 2121
Size: 105 348 252 420 672 324
Fragments arranged by size:
672 420 348 324 252 105
AflIII A'CryG_T
Cuts at: 0 529 620 958 2121
Size: 529 91 338 1163
Fragments arranged by size:
1163 529 338 91
AlwNI CAG_nnn'CTG
Cuts at: 0 1680 2121
Size: 1680 441
ApaI G_GGCC'C
Cuts at: 0 1404 2121
Size: 1404 717
ApaLI G'TGCA_C
Cuts at: 0 1765 2121
Size: 1765 356
ApoI r'AATT_y
Cuts at: 0 228 634 1233 1485 1737 1905 2121
Size: 228 406 599 252 252 168 216
Fragments arranged by size:
599 406 252 252 228 216 168
BanII G_rGCy'C
Cuts at: 0 1404 2121
Size: 1404 717
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 211 2121
Size: 39 172 1910
Fragments arranged by size:
1910 172 39
BciVI GTATCCnnnnn_n'
Cuts at: 0 156 2121
Size: 156 1965
BfrBI ATG'CAT
Cuts at: 0 2007 2121
Size: 2007 114
BglII A'GATC_T
Cuts at: 0 1071 2121
Size: 1071 1050
Bme1580I G_kGCm'C
Cuts at: 0 1404 1769 2121
Size: 1404 365 352
Fragments arranged by size:
1404 365 352
BmgBI CAC'GTC
Cuts at: 0 36 621 2121
Size: 36 585 1500
Fragments arranged by size:
1500 585 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1496 2121
Size: 1496 625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 701 1541 1793 2121
Size: 133 568 840 252 328
Fragments arranged by size:
840 568 328 252 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 2061 2121
Size: 326 1735 60
Fragments arranged by size:
1735 326 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 344 1605 2121
Size: 344 1261 516
Fragments arranged by size:
1261 516 344
BsaAI yAC'GTr
Cuts at: 0 530 959 2121
Size: 530 429 1162
Fragments arranged by size:
1162 530 429
BsaBI GATnn'nnATC
Cuts at: 0 494 2006 2121
Size: 494 1512 115
Fragments arranged by size:
1512 494 115
BsaHI Gr'CG_yC
Cuts at: 0 1848 2121
Size: 1848 273
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1054 2121
Size: 1054 1067
BseYI C'CCAG_C
Cuts at: 0 63 818 1649 2121
Size: 63 755 831 472
Fragments arranged by size:
831 755 472 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1666 2121
Size: 1666 455
BsiHKAI G_wGCw'C
Cuts at: 0 1769 2121
Size: 1769 352
BsmI GAATG_Cn'
Cuts at: 0 151 796 915 2121
Size: 151 645 119 1206
Fragments arranged by size:
1206 645 151 119
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1839 2121
Size: 1839 282
Bsp1286I G_dGCh'C
Cuts at: 0 1404 1769 2121
Size: 1404 365 352
Fragments arranged by size:
1404 365 352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 2121
Size: 137 1984
BsrDI GCAATG_nn'
Cuts at: 0 26 456 2121
Size: 26 430 1665
Fragments arranged by size:
1665 430 26
BsrGI T'GTAC_A
Cuts at: 0 1899 2121
Size: 1899 222
BssSI C'ACGA_G
Cuts at: 0 1152 1656 2121
Size: 1152 504 465
Fragments arranged by size:
1152 504 465
BstXI CCAn_nnnn'nTGG
Cuts at: 0 451 2121
Size: 451 1670
BstYI r'GATC_y
Cuts at: 0 242 1071 1258 1323 2121
Size: 242 829 187 65 798
Fragments arranged by size:
829 798 242 187 65
Bsu36I CC'TnA_GG
Cuts at: 0 178 2121
Size: 178 1943
BtgI C'CryG_G
Cuts at: 0 920 2121
Size: 920 1201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 985 2121
Size: 985 1136
EarI CTCTTCn'nnn_
Cuts at: 0 53 1129 1381 1454 1465 1633 1706 2121
Size: 53 1076 252 73 11 168 73 415
Fragments arranged by size:
1076 415 252 168 73 73 53 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 133 453 701 705 1125 1541 1793
Size: 105 28 320 248 4 420 416 252
Cuts at: 1793 1797 2121
Size: 4 324
Fragments arranged by size:
420 416 324 320 252 248 105 28
4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 1401 2121
Size: 1401 720
EcoRI G'AATT_C
Cuts at: 0 228 634 1737 1905 2121
Size: 228 406 1103 168 216
Fragments arranged by size:
1103 406 228 216 168
HaeII r_GCGC'y
Cuts at: 0 1992 2121
Size: 1992 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 227 259 636 668 960 992 1308 1340
Size: 227 32 377 32 292 32 316 32
Cuts at: 1340 1380 1412 2121
Size: 40 32 709
Fragments arranged by size:
709 377 316 292 227 40 32 32
32 32 32
MfeI C'AATT_G
Cuts at: 0 1620 2121
Size: 1620 501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1301 2088 2121
Size: 1301 787 33
Fragments arranged by size:
1301 787 33
MslI CAynn'nnrTG
Cuts at: 0 530 864 948 1032 1200 1284 1452 1536
Size: 530 334 84 84 168 84 168 84
Cuts at: 1536 1704 1788 1956 2040 2096 2121
Size: 168 84 168 84 56 25
Fragments arranged by size:
530 334 168 168 168 168 84 84
84 84 84 84 56 25
MspA1I CmG'CkG
Cuts at: 0 1817 2121
Size: 1817 304
NcoI C'CATG_G
Cuts at: 0 920 2121
Size: 920 1201
NdeI CA'TA_TG
Cuts at: 0 1088 2121
Size: 1088 1033
NsiI A_TGCA'T
Cuts at: 0 2009 2121
Size: 2009 112
PasI CC'CwG_GG
Cuts at: 0 444 2121
Size: 444 1677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 676 708 2121
Size: 118 32 526 32 1413
Fragments arranged by size:
1413 526 118 32 32
PsiI TTA'TAA
Cuts at: 0 779 2121
Size: 779 1342
PspOMI G'GGCC_C
Cuts at: 0 1400 2121
Size: 1400 721
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 541 573 2121
Size: 541 32 1548
Fragments arranged by size:
1548 541 32
PvuII CAG'CTG
Cuts at: 0 1817 2121
Size: 1817 304
SapI GCTCTTCn'nnn_
Cuts at: 0 53 1129 1381 2121
Size: 53 1076 252 740
Fragments arranged by size:
1076 740 252 53
SexAI A'CCwGG_T
Cuts at: 0 334 2121
Size: 334 1787
SmlI C'TyrA_G
Cuts at: 0 341 2076 2121
Size: 341 1735 45
Fragments arranged by size:
1735 341 45
StuI AGG'CCT
Cuts at: 0 802 2074 2121
Size: 802 1272 47
Fragments arranged by size:
1272 802 47
StyI C'CwwG_G
Cuts at: 0 920 2121
Size: 920 1201
TatI w'GTAC_w
Cuts at: 0 1899 2121
Size: 1899 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1243 1275 2121
Size: 1243 32 846
Fragments arranged by size:
1243 846 32
XmnI GAAnn'nnTTC
Cuts at: 0 691 718 802 859 943 1138 1810 1909
Size: 691 27 84 57 84 195 672 99
Cuts at: 1909 1931 2121
Size: 22 190
Fragments arranged by size:
691 672 195 190 99 84 84 57
27 22
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApaLI ApoI BanII BbsI
BciVI BfrBI BglII Bme1580I BmgBI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI BstXI
BstYI Bsu36I BtgI BtgZI EarI Eco57MI EcoO109I EcoRI
HaeII Hin4I MfeI MmeI MslI MspA1I NcoI NdeI
NsiI PasI PpiI PsiI PspOMI PsrI PvuII SapI
SexAI SmlI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI
BglI BlpI BmrI BmtI Bpu10I BsaWI BsaXI BsiEI
BsiWI BspEI BspHI BsrBI BsrFI BssHII BstAPI BstBI
BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
MscI NaeI NarI NgoMIV NheI NotI NruI NspI
PacI PciI PflMI PfoI PmeI PmlI PpuMI PshAI
PspXI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI ZraI