(Circular) (Six-base) MAPSORT of: pFN21AB1123.seq Check: 531 from: 1 to: 12352 pFN21AB1123 12352 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3424 3887 4507 3424 Size: 463 620 11269 Fragments arranged by size: 11269 620 463 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7870 10162 278 Size: 53 83 186 936 6334 2292 2468 Fragments arranged by size: 6334 2468 2292 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2183 7949 9695 1937 Size: 246 5766 1746 4594 Fragments arranged by size: 5766 4594 1746 246 Acc65I G'GTAC_C Cuts at: 7902 9673 7902 Size: 1771 10581 AclI AA'CG_TT Cuts at: 1563 10479 10852 1563 Size: 8916 373 3063 Fragments arranged by size: 8916 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4352 4640 5400 5900 6078 8009 8219 10415 Size: 2720 288 760 500 178 1931 210 2196 Cuts at: 10415 11799 12067 1632 Size: 1384 268 1917 Fragments arranged by size: 2720 2196 1931 1917 1384 760 500 288 268 210 178 AfeI AGC'GCT Cuts at: 4049 5409 5627 4049 Size: 1360 218 10774 Fragments arranged by size: 10774 1360 218 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 12155 AflIII A'CryG_T Cuts at: 1203 7113 7995 11251 12228 1203 Size: 5910 882 3256 977 1327 Fragments arranged by size: 5910 3256 1327 977 882 AgeI A'CCGG_T Cuts at: 11183 11183 Size: 12352 AhdI GACnn_n'nnGTC Cuts at: 5533 11081 5533 Size: 5548 6804 AleI CACnn'nnGTG Cuts at: 1166 3071 3560 6688 1166 Size: 1905 489 3128 6830 Fragments arranged by size: 6830 3128 1905 489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2094 3003 3482 6164 6706 6997 8825 1184 Size: 910 909 479 2682 542 291 1828 4711 Fragments arranged by size: 4711 2682 1828 910 909 542 479 291 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5024 5056 6198 6230 5024 Size: 32 1142 32 11146 Fragments arranged by size: 11146 1142 32 32 AlwNI CAG_nnn'CTG Cuts at: 2884 4004 4367 4972 5050 7231 8197 10095 11667 Size: 1120 363 605 78 2181 966 1898 1572 Cuts at: 11667 2884 Size: 3569 Fragments arranged by size: 3569 2181 1898 1572 1120 966 605 363 78 ApaI G_GGCC'C Cuts at: 5920 6116 8189 5920 Size: 196 2073 10083 Fragments arranged by size: 10083 2073 196 ApaLI G'TGCA_C Cuts at: 10409 11565 10409 Size: 1156 11196 ApoI r'AATT_y Cuts at: 1493 2441 3110 3782 4612 5033 5123 5201 5571 Size: 948 669 672 830 421 90 78 370 Cuts at: 5571 6089 9372 9661 9887 10039 12148 1493 Size: 518 3283 289 226 152 2109 1697 Fragments arranged by size: 3283 2109 1697 948 830 672 669 518 421 370 289 226 152 90 78 AseI AT'TA_AT Cuts at: 160 1032 4340 6261 10906 160 Size: 872 3308 1921 4645 1606 Fragments arranged by size: 4645 3308 1921 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12352 AvaI C'yCGr_G Cuts at: 1870 1943 3474 4731 6455 6615 6830 8704 9105 Size: 73 1531 1257 1724 160 215 1874 401 Cuts at: 9105 9677 1870 Size: 572 4545 Fragments arranged by size: 4545 1874 1724 1531 1257 572 401 215 160 73 BamHI G'GATC_C Cuts at: 2784 4687 5686 9682 10049 2784 Size: 1903 999 3996 367 5087 Fragments arranged by size: 5087 3996 1903 999 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4278 5315 7648 7902 9639 Size: 325 217 627 2491 1037 2333 254 1737 Cuts at: 9639 9673 11128 618 Size: 34 1455 1842 Fragments arranged by size: 2491 2333 1842 1737 1455 1037 627 325 254 217 34 BanII G_rGCy'C Cuts at: 1391 2136 2461 3525 4575 5504 5920 6015 6116 Size: 745 325 1064 1050 929 416 95 101 Cuts at: 6116 8163 8189 9671 1391 Size: 2047 26 1482 4072 Fragments arranged by size: 4072 2047 1482 1064 1050 929 745 416 325 101 95 26 BbeI G_GCGC'C Cuts at: 5319 5319 Size: 12352 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3442 4628 5239 5529 6954 928 Size: 971 1543 1186 611 290 1425 6326 Fragments arranged by size: 6326 1543 1425 1186 971 611 290 BbvCI CC'TCA_GC Cuts at: 7824 8352 8663 7824 Size: 528 311 11513 Fragments arranged by size: 11513 528 311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2668 2702 3964 3998 10543 Size: 34 50 34 647 34 1262 34 6545 Cuts at: 10543 10577 1903 Size: 34 3678 Fragments arranged by size: 6545 3678 1262 647 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5654 7702 10244 11454 5654 Size: 2048 2542 1210 6552 Fragments arranged by size: 6552 2542 2048 1210 BclI T'GATC_A Cuts at: 1548 3115 4386 1548 Size: 1567 1271 9514 Fragments arranged by size: 9514 1567 1271 BfrBI ATG'CAT Cuts at: 5254 5735 5254 Size: 481 11871 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3669 6386 6539 Size: 107 122 71 631 605 1997 2717 153 Cuts at: 6539 6548 6984 7469 7505 8715 9183 10963 136 Size: 9 436 485 36 1210 468 1780 1525 Fragments arranged by size: 2717 1997 1780 1525 1210 631 605 485 468 436 153 122 107 71 36 9 BglII A'GATC_T Cuts at: 2908 6855 8575 12347 2908 Size: 3947 1720 3772 2913 Fragments arranged by size: 3947 3772 2913 1720 BlpI GC'TnA_GC Cuts at: 3971 5864 9769 3971 Size: 1893 3905 6554 Fragments arranged by size: 6554 3905 1893 Bme1580I G_kGCm'C Cuts at: 1790 4267 4281 5920 6116 7129 8189 9644 10413 Size: 2477 14 1639 196 1013 1060 1455 769 Cuts at: 10413 11569 1790 Size: 1156 2573 Fragments arranged by size: 2573 2477 1639 1455 1156 1060 1013 769 196 14 BmgBI CAC'GTC Cuts at: 1318 5782 1318 Size: 4464 7888 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2357 3045 3229 Size: 416 53 83 399 24 940 688 184 Cuts at: 3229 10083 11041 442 Size: 6854 958 1753 Fragments arranged by size: 6854 1753 958 940 688 416 399 184 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2280 1056 Size: 1224 11128 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9542 9542 Size: 12352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 3539 4472 5891 6230 6353 6821 7688 Size: 881 1419 933 1419 339 123 468 867 Cuts at: 7688 8177 9310 11012 1239 Size: 489 1133 1702 2579 Fragments arranged by size: 2579 1702 1419 1419 1133 933 881 867 489 468 339 123 Bpu10I CC'TnA_GC Cuts at: 3266 3575 5714 7824 8352 8663 3266 Size: 309 2139 2110 528 311 6955 Fragments arranged by size: 6955 2139 2110 528 311 309 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4619 6950 10477 11342 11640 4619 Size: 2331 3527 865 298 5331 Fragments arranged by size: 5331 3527 2331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2738 4291 4878 5833 6518 11015 882 Size: 617 1239 1553 587 955 685 4497 2219 Fragments arranged by size: 4497 2219 1553 1239 955 685 617 587 BsaAI yAC'GTr Cuts at: 493 1204 2437 10143 493 Size: 711 1233 7706 2702 Fragments arranged by size: 7706 2702 1233 711 BsaBI GATnn'nnATC Cuts at: 4205 4874 12346 4205 Size: 669 7472 4211 Fragments arranged by size: 7472 4211 669 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3441 4034 5316 7867 Size: 53 83 186 936 1908 593 1282 2551 Cuts at: 7867 10159 10541 11176 275 Size: 2292 382 635 1451 Fragments arranged by size: 2551 2292 1908 1451 1282 936 635 593 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2015 8821 10785 11183 11457 11604 12015 2015 Size: 6806 1964 398 274 147 411 2352 Fragments arranged by size: 6806 2352 1964 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3068 3098 4271 4301 5444 Size: 30 165 30 1665 30 1173 30 1143 Cuts at: 5444 5474 6659 6689 1178 Size: 30 1185 30 6841 Fragments arranged by size: 6841 1665 1185 1173 1143 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2336 3119 5696 6818 8060 1186 Size: 1006 144 783 2577 1122 1242 5478 Fragments arranged by size: 5478 2577 1242 1122 1006 783 144 BseYI C'CCAG_C Cuts at: 2470 2667 2976 3004 4273 4846 5050 5220 5554 Size: 197 309 28 1269 573 204 170 334 Cuts at: 5554 5913 6151 6436 7188 8718 8888 8927 11555 Size: 359 238 285 752 1530 170 39 2628 Cuts at: 11555 12051 2470 Size: 496 2771 Fragments arranged by size: 2771 2628 1530 1269 752 573 496 359 334 309 285 238 204 197 170 170 39 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4126 4985 5762 6347 7217 8278 8726 Size: 573 2390 859 777 585 870 1061 448 Cuts at: 8726 9050 1163 Size: 324 4465 Fragments arranged by size: 4465 2390 1061 870 859 777 585 573 448 324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6294 7354 7509 9802 10563 11591 Size: 275 186 4293 1060 155 2293 761 1028 Cuts at: 11591 1540 Size: 2301 Fragments arranged by size: 4293 2301 2293 1060 1028 761 275 186 155 BsiHKAI G_wGCw'C Cuts at: 2136 3567 3849 4233 4575 5156 5640 6015 7307 Size: 1431 282 384 342 581 484 375 1292 Cuts at: 7307 7350 7571 8007 9234 10413 10498 11569 2136 Size: 43 221 436 1227 1179 85 1071 2919 Fragments arranged by size: 2919 1431 1292 1227 1179 1071 581 484 436 384 375 342 282 221 85 43 BsmI GAATG_Cn' Cuts at: 2308 3206 8460 9863 9956 2308 Size: 898 5254 1403 93 4704 Fragments arranged by size: 5254 4704 1403 898 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5851 7053 7874 9450 5851 Size: 1202 821 1576 8753 Fragments arranged by size: 8753 1576 1202 821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2461 3525 3567 3849 4233 4267 Size: 399 346 325 1064 42 282 384 34 Cuts at: 4267 4281 4575 5156 5504 5640 5920 6015 6116 Size: 14 294 581 348 136 280 95 101 Cuts at: 6116 7129 7307 7350 7571 8007 8163 8189 9234 Size: 1013 178 43 221 436 156 26 1045 Cuts at: 9234 9644 9671 10413 10498 11569 1391 Size: 410 27 742 85 1071 2174 Fragments arranged by size: 2174 1071 1064 1045 1013 742 581 436 410 399 384 348 346 325 294 282 280 221 178 156 136 101 95 85 43 42 34 27 26 14 BspEI T'CCGG_A Cuts at: 8821 12015 8821 Size: 3194 9158 BspHI T'CATG_A Cuts at: 5115 5133 6943 9547 10241 11971 5115 Size: 18 1810 2604 694 1730 5496 Fragments arranged by size: 5496 2604 1810 1730 694 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2029 3424 3887 4507 5223 5343 6720 9707 Size: 1185 1395 463 620 716 120 1377 2987 Cuts at: 9707 844 Size: 3489 Fragments arranged by size: 3489 2987 1395 1377 1185 716 620 463 120 BsrBI CCG'CTC Cuts at: 1394 1823 3386 10239 1394 Size: 429 1563 6853 3507 Fragments arranged by size: 6853 3507 1563 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2062 2491 3435 3486 6741 7830 10847 Size: 1183 814 429 944 51 3255 1089 3017 Cuts at: 10847 11021 65 Size: 174 1396 Fragments arranged by size: 3255 3017 1396 1183 1089 944 814 429 174 51 BsrFI r'CCGG_y Cuts at: 1700 1810 3081 10996 11183 1700 Size: 110 1271 7915 187 2869 Fragments arranged by size: 7915 2869 1271 187 110 BsrGI T'GTAC_A Cuts at: 96 2968 7641 96 Size: 2872 4673 4807 Fragments arranged by size: 4807 4673 2872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12352 BssSI C'ACGA_G Cuts at: 6365 7689 10412 11424 6365 Size: 1324 2723 1012 7293 Fragments arranged by size: 7293 2723 1324 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4972 5344 5710 6450 7231 794 Size: 4178 372 366 740 781 5915 Fragments arranged by size: 5915 4178 781 740 372 366 BstBI TT'CG_AA Cuts at: 5485 7398 11156 5485 Size: 1913 3758 6681 Fragments arranged by size: 6681 3758 1913 BstEII G'GTnAC_C Cuts at: 1184 7090 1184 Size: 5906 6446 BstXI CCAn_nnnn'nTGG Cuts at: 3073 3685 3739 5312 5524 7858 9163 3073 Size: 612 54 1573 212 2334 1305 6262 Fragments arranged by size: 6262 2334 1573 1305 612 212 54 BstYI r'GATC_y Cuts at: 1972 2784 2908 3855 4000 4122 4357 4687 4875 Size: 812 124 947 145 122 235 330 188 Cuts at: 4875 5686 5950 6336 6855 7618 8575 8778 9060 Size: 811 264 386 519 763 957 203 282 Cuts at: 9060 9682 10049 10434 10451 11903 11989 12001 12347 Size: 622 367 385 17 1452 86 12 346 Cuts at: 12347 1972 Size: 1977 Fragments arranged by size: 1977 1452 957 947 812 811 763 622 519 386 385 367 346 330 282 264 235 203 188 145 124 122 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3726 7086 3726 Size: 3360 8992 BtgI C'CryG_G Cuts at: 513 1065 2151 2406 2788 4857 4911 5523 6771 Size: 552 1086 255 382 2069 54 612 1248 Cuts at: 6771 7503 513 Size: 732 5362 Fragments arranged by size: 5362 2069 1248 1086 732 612 552 382 255 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3750 5877 6037 7183 7452 7575 Size: 683 56 2524 2127 160 1146 269 123 Cuts at: 7575 8783 487 Size: 1208 4056 Fragments arranged by size: 4056 2524 2127 1208 1146 683 269 160 123 56 BtsI GCAGTG_nn' Cuts at: 3540 3555 3567 3954 5387 6497 6656 7392 7491 Size: 15 12 387 1433 1110 159 736 99 Cuts at: 7491 7512 7524 8738 9868 10662 10682 3540 Size: 21 12 1214 1130 794 20 5210 Fragments arranged by size: 5210 1433 1214 1130 1110 794 736 387 159 99 21 20 15 12 12 ClaI AT'CG_AT Cuts at: 1554 7137 1554 Size: 5583 6769 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2296 2331 3417 3452 529 Size: 35 1732 35 1086 35 9429 Fragments arranged by size: 9429 1732 1086 35 35 35 DraI TTT'AAA Cuts at: 9656 9656 Size: 12352 DraIII CAC_nnn'GTG Cuts at: 4705 9211 4705 Size: 4506 7846 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2366 3446 11359 809 Size: 507 1050 1080 7913 1802 Fragments arranged by size: 7913 1802 1080 1050 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2268 2395 2529 Size: 54 1425 50 275 18 438 127 134 Cuts at: 2529 2592 2751 3919 4134 4506 4548 5722 5943 Size: 63 159 1168 215 372 42 1174 221 Cuts at: 5943 6291 7506 7751 8061 8631 8901 9249 9358 Size: 348 1215 245 310 570 270 348 109 Cuts at: 9358 9799 10688 12317 8 Size: 441 889 1629 43 Fragments arranged by size: 1629 1425 1215 1174 1168 889 570 441 438 372 348 348 310 275 270 245 221 215 159 134 127 109 63 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 6291 7506 9799 1537 Size: 275 4479 1215 2293 4090 Fragments arranged by size: 4479 4090 2293 1215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5069 5673 5960 6677 7115 8094 8612 8655 Size: 3716 604 287 717 438 979 518 43 Cuts at: 8655 10282 1353 Size: 1627 3423 Fragments arranged by size: 3716 3423 1627 979 717 604 518 438 287 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4088 4298 6839 10939 11313 11459 Size: 1064 138 2499 210 2541 4100 374 146 Cuts at: 11459 387 Size: 1280 Fragments arranged by size: 4100 2541 2499 1280 1064 374 210 146 138 EcoICRI GAG'CTC Cuts at: 2134 4573 6013 2134 Size: 2439 1440 8473 Fragments arranged by size: 8473 2439 1440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 3539 4352 4472 4640 5400 5891 Size: 393 488 1419 813 120 168 760 491 Cuts at: 5891 5900 6078 6230 6353 6821 7688 8009 8177 Size: 9 178 152 123 468 867 321 168 Cuts at: 8177 8219 9310 10415 11012 11799 12067 1239 Size: 42 1091 1105 597 787 268 1524 Fragments arranged by size: 1524 1419 1105 1091 867 813 787 760 597 491 488 468 393 321 268 178 168 168 152 123 120 42 9 EcoNI CCTnn'n_nnAGG Cuts at: 3725 4067 4976 5801 5924 8099 3725 Size: 342 909 825 123 2175 7978 Fragments arranged by size: 7978 2175 909 825 342 123 EcoO109I rG'GnC_Cy Cuts at: 2602 3042 3682 5762 5917 6112 6113 6224 6667 Size: 440 640 2080 155 195 1 111 443 Cuts at: 6667 6709 7915 8185 8186 8290 8308 8407 8508 Size: 42 1206 270 1 104 18 99 101 Cuts at: 8508 9478 2602 Size: 970 5476 Fragments arranged by size: 5476 2080 1206 970 640 443 440 270 195 155 111 104 101 99 42 18 1 1 EcoRI G'AATT_C Cuts at: 5123 6089 9661 10039 5123 Size: 966 3572 378 7436 Fragments arranged by size: 7436 3572 966 378 EcoRV GAT'ATC Cuts at: 3505 3505 Size: 12352 FspI TGC'GCA Cuts at: 4196 10858 4196 Size: 6662 5690 HaeII r_GCGC'y Cuts at: 1678 4051 5319 5411 5629 5817 7338 11499 1678 Size: 2373 1268 92 218 188 1521 4161 2531 Fragments arranged by size: 4161 2531 2373 1521 1268 218 188 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3985 4017 4641 4673 5444 Size: 32 135 32 2413 32 624 32 771 Cuts at: 5444 5476 5519 5551 5598 5630 5876 5908 6659 Size: 32 43 32 47 32 246 32 751 Cuts at: 6659 6691 6739 6771 6828 6831 6860 6863 7607 Size: 32 48 32 57 3 29 3 744 Cuts at: 7607 7639 8049 8081 8630 8662 8667 8699 9335 Size: 32 410 32 549 32 5 32 636 Cuts at: 9335 9367 10993 11025 11067 11099 1373 Size: 32 1626 32 42 32 2626 Fragments arranged by size: 2626 2413 1626 771 751 744 636 624 549 410 246 135 57 48 47 43 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 5 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 4813 6479 8620 9153 9696 9942 Size: 988 281 2875 1666 2141 533 543 246 Cuts at: 9942 669 Size: 3079 Fragments arranged by size: 3079 2875 2141 1666 988 543 533 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 12352 HpaI GTT'AAC Cuts at: 8620 9942 8620 Size: 1322 11030 KasI G'GCGC_C Cuts at: 5315 5315 Size: 12352 KpnI G_GTAC'C Cuts at: 7906 9677 7906 Size: 1771 10581 MfeI C'AATT_G Cuts at: 9951 9951 Size: 12352 MluI A'CGCG_T Cuts at: 12228 12228 Size: 12352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3235 4206 8353 9054 9593 11466 11650 1305 Size: 1930 971 4147 701 539 1873 184 2007 Fragments arranged by size: 4147 2007 1930 1873 971 701 539 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 2531 2753 3921 4136 Size: 54 1425 343 438 261 222 1168 215 Cuts at: 4136 4508 4550 5724 5945 7753 8063 8633 8903 Size: 372 42 1174 221 1808 310 570 270 Cuts at: 8903 9360 10 Size: 457 3002 Fragments arranged by size: 3002 1808 1425 1174 1168 570 457 438 372 343 310 270 261 222 221 215 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2288 2375 2588 3071 3464 3560 3927 Size: 648 1122 87 213 483 393 96 367 Cuts at: 3927 4481 4661 4715 5114 5522 5582 6605 6688 Size: 554 180 54 399 408 60 1023 83 Cuts at: 6688 8036 8987 9212 9251 10310 10669 10828 518 Size: 1348 951 225 39 1059 359 159 2042 Fragments arranged by size: 2042 1348 1122 1059 1023 951 648 554 483 408 399 393 367 359 225 213 180 159 96 87 83 60 54 39 MspA1I CmG'CkG Cuts at: 1606 2039 2429 2507 2884 3299 3697 4850 5050 Size: 433 390 78 377 415 398 1153 200 Cuts at: 5050 5224 5308 5554 6290 6560 7279 7359 7467 Size: 174 84 246 736 270 719 80 108 Cuts at: 7467 7597 8347 8888 9768 10445 11593 11838 1606 Size: 130 750 541 880 677 1148 245 2120 Fragments arranged by size: 2120 1153 1148 880 750 736 719 677 541 433 415 398 390 377 270 246 245 200 174 130 108 84 80 78 NarI GG'CG_CC Cuts at: 5316 5316 Size: 12352 NcoI C'CATG_G Cuts at: 513 1065 2151 2406 2788 4857 4911 5523 6771 Size: 552 1086 255 382 2069 54 612 1248 Cuts at: 6771 513 Size: 6094 Fragments arranged by size: 6094 2069 1248 1086 612 552 382 255 54 NdeI CA'TA_TG Cuts at: 387 6181 6763 8434 387 Size: 5794 582 1671 4305 Fragments arranged by size: 5794 4305 1671 582 NheI G'CTAG_C Cuts at: 1052 2276 1052 Size: 1224 11128 NotI GC'GGCC_GC Cuts at: 9799 9799 Size: 12352 NsiI A_TGCA'T Cuts at: 5256 5737 5256 Size: 481 11871 NspI r_CATG'y Cuts at: 1132 1229 2504 3469 3676 4273 4486 5239 6448 Size: 97 1275 965 207 597 213 753 1209 Cuts at: 6448 6790 7117 7156 7196 7379 7999 8146 8171 Size: 342 327 39 40 183 620 147 25 Cuts at: 8171 8873 9204 9710 11255 1132 Size: 702 331 506 1545 2229 Fragments arranged by size: 2229 1545 1275 1209 965 753 702 620 597 506 342 331 327 213 207 183 147 97 40 39 25 PasI CC'CwG_GG Cuts at: 1348 3313 4534 4948 1348 Size: 1965 1221 414 8752 Fragments arranged by size: 8752 1965 1221 414 PciI A'CATG_T Cuts at: 7113 7995 11251 7113 Size: 882 3256 8214 Fragments arranged by size: 8214 3256 882 PflMI CCAn_nnn'nTGG Cuts at: 1108 3313 3697 5050 8070 1108 Size: 2205 384 1353 3020 5390 Fragments arranged by size: 5390 3020 2205 1353 384 PfoI T'CCnGG_A Cuts at: 4125 4488 5028 5462 7704 8754 4125 Size: 363 540 434 2242 1050 7723 Fragments arranged by size: 7723 2242 1050 540 434 363 PmeI GTTT'AAAC Cuts at: 9656 9656 Size: 12352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2917 2949 10421 10453 11934 11966 2917 Size: 32 7472 32 1481 32 3303 Fragments arranged by size: 7472 3303 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2602 3042 5762 6667 6709 8290 8308 8407 2602 Size: 440 2720 905 42 1581 18 99 6547 Fragments arranged by size: 6547 2720 1581 905 440 99 42 18 PshAI GACnn'nnGTC Cuts at: 1613 7896 1613 Size: 6283 6069 PsiI TTA'TAA Cuts at: 9922 9922 Size: 12352 PspOMI G'GGCC_C Cuts at: 5916 6112 8185 5916 Size: 196 2073 10083 Fragments arranged by size: 10083 2073 196 PspXI vC'TCGA_Gb Cuts at: 3474 6455 3474 Size: 2981 9371 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 8947 8979 8947 Size: 32 12320 PstI C_TGCA'G Cuts at: 830 2073 3562 5648 6559 6876 7326 7602 9704 Size: 1243 1489 2086 911 317 450 276 2102 Cuts at: 9704 830 Size: 3478 Fragments arranged by size: 3478 2102 2086 1489 1243 911 450 317 276 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12352 PvuII CAG'CTG Cuts at: 2507 2884 3697 4850 5050 5224 5308 5554 6560 Size: 377 813 1153 200 174 84 246 1006 Cuts at: 6560 7279 7359 8347 8888 2507 Size: 719 80 988 541 5971 Fragments arranged by size: 5971 1153 1006 988 813 719 541 377 246 200 174 84 80 SacI G_AGCT'C Cuts at: 2136 4575 6015 2136 Size: 2439 1440 8473 Fragments arranged by size: 8473 2439 1440 SalI G'TCGA_C Cuts at: 1936 9694 1936 Size: 7758 4594 SanDI GG'GwC_CC Cuts at: 2602 2602 Size: 12352 SapI GCTCTTCn'nnn_ Cuts at: 5069 8612 5069 Size: 3543 8809 SbfI CC_TGCA'GG Cuts at: 9704 9704 Size: 12352 ScaI AGT'ACT Cuts at: 2900 7328 10600 12312 2900 Size: 4428 3272 1712 2940 Fragments arranged by size: 4428 3272 2940 1712 SexAI A'CCwGG_T Cuts at: 4110 4281 6347 4110 Size: 171 2066 10115 Fragments arranged by size: 10115 2066 171 SfcI C'TryA_G Cuts at: 826 1045 2069 2631 3558 4576 4789 5644 6272 Size: 219 1024 562 927 1018 213 855 628 Cuts at: 6272 6555 6872 7286 7322 7330 7598 7811 8761 Size: 283 317 414 36 8 268 213 950 Cuts at: 8761 9190 9700 10835 11516 11707 826 Size: 429 510 1135 681 191 1471 Fragments arranged by size: 1471 1135 1024 1018 950 927 855 681 628 562 510 429 414 317 283 268 219 213 213 191 36 8 SfoI GGC'GCC Cuts at: 5317 5317 Size: 12352 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12352 SmaI CCC'GGG Cuts at: 1872 4733 9679 1872 Size: 2861 4946 4545 Fragments arranged by size: 4946 4545 2861 SmlI C'TyrA_G Cuts at: 820 1017 1943 3474 4598 6455 6965 10456 11357 Size: 197 926 1531 1124 1857 510 3491 901 Cuts at: 11357 11619 820 Size: 262 1553 Fragments arranged by size: 3491 1857 1553 1531 1124 926 901 510 262 197 SnaBI TAC'GTA Cuts at: 493 10143 493 Size: 9650 2702 SpeI A'CTAG_T Cuts at: 152 4796 152 Size: 4644 7708 SphI G_CATG'C Cuts at: 1132 3469 3676 4273 6448 7196 7379 8171 8873 Size: 2337 207 597 2175 748 183 792 702 Cuts at: 8873 9710 1132 Size: 837 3774 Fragments arranged by size: 3774 2337 2175 837 792 748 702 597 207 183 SspI AAT'ATT Cuts at: 5 52 10276 5 Size: 47 10224 2081 Fragments arranged by size: 10224 2081 47 StuI AGG'CCT Cuts at: 1517 3027 4088 5807 5930 6461 7370 7769 8094 Size: 1510 1061 1719 123 531 909 399 325 Cuts at: 8094 8222 9009 9177 1517 Size: 128 787 168 4692 Fragments arranged by size: 4692 1719 1510 1061 909 787 531 399 325 168 128 123 StyI C'CwwG_G Cuts at: 513 1065 2127 2151 2385 2406 2748 2788 2991 Size: 552 1062 24 234 21 342 40 203 Cuts at: 2991 3022 3678 4857 4911 5523 5725 6462 6678 Size: 31 656 1179 54 612 202 737 216 Cuts at: 6678 6771 7857 9791 513 Size: 93 1086 1934 3074 Fragments arranged by size: 3074 1934 1179 1086 1062 737 656 612 552 342 234 216 203 202 93 54 40 31 24 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2462 3121 3681 6143 6638 8312 8964 Size: 1255 882 659 560 2462 495 1674 652 Cuts at: 8964 9039 10379 10396 10549 10734 11179 325 Size: 75 1340 17 153 185 445 1498 Fragments arranged by size: 2462 1674 1498 1340 1255 882 659 652 560 495 445 185 153 75 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2898 2968 6816 Size: 274 80 33 51 1443 921 70 3848 Cuts at: 6816 7326 7641 8115 8265 10598 12310 96 Size: 510 315 474 150 2333 1712 138 Fragments arranged by size: 3848 2333 1712 1443 921 510 474 315 274 150 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3068 3100 3888 3920 4271 4303 4530 4562 5046 Size: 32 788 32 351 32 227 32 484 Cuts at: 5046 5078 6776 6808 12312 12344 3068 Size: 32 1698 32 5504 32 3076 Fragments arranged by size: 5504 3076 1698 788 484 351 227 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5434 9242 1316 Size: 4118 3808 4426 Fragments arranged by size: 4426 4118 3808 XbaI T'CTAG_A Cuts at: 6241 9688 6241 Size: 3447 8905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3532 3914 4144 4516 6712 7858 9037 9629 3532 Size: 382 230 372 2196 1146 1179 592 6255 Fragments arranged by size: 6255 2196 1179 1146 592 382 372 230 XhoI C'TCGA_G Cuts at: 1943 3474 6455 1943 Size: 1531 2981 7840 Fragments arranged by size: 7840 2981 1531 XmaI C'CCGG_G Cuts at: 1870 4731 9677 1870 Size: 2861 4946 4545 Fragments arranged by size: 4946 4545 2861 XmnI GAAnn'nnTTC Cuts at: 9438 10481 9438 Size: 1043 11309 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7868 10160 276 Size: 53 83 186 936 6334 2292 2468 Fragments arranged by size: 6334 2468 2292 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI RsrII SacII SfiI SrfI SwaI