Restriction map for
pFN21AB0179
(Linear) (Six-base) MAPSORT of: KIBB0179.seq Check: 4701 from: 1 to: 3660
KIBB0179.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3381 3660
Size: 3381 279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1157 1729 3134 3660
Size: 364 793 572 1405 526
Fragments arranged by size:
1405 793 572 526 364
AleI CACnn'nnGTG
Cuts at: 0 2083 2746 3660
Size: 2083 663 914
Fragments arranged by size:
2083 914 663
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 506 3660
Size: 506 3154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2393 2425 3660
Size: 2393 32 1235
Fragments arranged by size:
2393 1235 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1749 1774 1803 3660
Size: 1749 25 29 1857
Fragments arranged by size:
1857 1749 29 25
ApaI G_GGCC'C
Cuts at: 0 2898 3225 3660
Size: 2898 327 435
Fragments arranged by size:
2898 435 327
ApaLI G'TGCA_C
Cuts at: 0 883 3660
Size: 883 2777
ApoI r'AATT_y
Cuts at: 0 151 226 1243 3100 3660
Size: 151 75 1017 1857 560
Fragments arranged by size:
1857 1017 560 151 75
AvaI C'yCGr_G
Cuts at: 0 73 635 3641 3660
Size: 73 562 3006 19
Fragments arranged by size:
3006 562 73 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 61 94 3008 3041 3660
Size: 61 33 2914 33 619
Fragments arranged by size:
2914 619 61 33 33
BanII G_rGCy'C
Cuts at: 0 785 899 1381 2898 3225 3660
Size: 785 114 482 1517 327 435
Fragments arranged by size:
1517 785 482 435 327 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 2325 3042 3660
Size: 2325 717 618
Fragments arranged by size:
2325 717 618
BbvCI CC'TCA_GC
Cuts at: 0 2148 3660
Size: 2148 1512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1853 1887 2513 2547 3660
Size: 1853 34 626 34 1113
Fragments arranged by size:
1853 1113 626 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3036 3660
Size: 3036 624
BclI T'GATC_A
Cuts at: 0 158 2861 3660
Size: 158 2703 799
Fragments arranged by size:
2703 799 158
BglII A'GATC_T
Cuts at: 0 1032 3660
Size: 1032 2628
BlpI GC'TnA_GC
Cuts at: 0 3565 3660
Size: 3565 95
Bme1580I G_kGCm'C
Cuts at: 0 887 1355 2898 3225 3420 3660
Size: 887 468 1543 327 195 240
Fragments arranged by size:
1543 887 468 327 240 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 352 989 1533 1791 2425 2444 2899 3660
Size: 352 637 544 258 634 19 455 761
Fragments arranged by size:
761 637 634 544 455 352 258 19
BmtI G_CTAG'C
Cuts at: 0 3139 3660
Size: 3139 521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1101 1167 3656 3660
Size: 1101 66 2489 4
Fragments arranged by size:
2489 1101 66 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 758 1046 2974 3651 3660
Size: 266 492 288 1928 677 9
Fragments arranged by size:
1928 677 492 288 266 9
Bpu10I CC'TnA_GC
Cuts at: 0 997 1911 2148 2645 3660
Size: 997 914 237 497 1015
Fragments arranged by size:
1015 997 914 497 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1696 2779 3660
Size: 1330 366 1083 881
Fragments arranged by size:
1330 1083 881 366
BsaI GGTCTCn'nnnn_
Cuts at: 0 581 1063 1075 3552 3660
Size: 581 482 12 2477 108
Fragments arranged by size:
2477 581 482 108 12
BsaBI GATnn'nnATC
Cuts at: 0 1013 1669 3660
Size: 1013 656 1991
Fragments arranged by size:
1991 1013 656
BsaHI Gr'CG_yC
Cuts at: 0 2629 3660
Size: 2629 1031
BsaWI w'CCGG_w
Cuts at: 0 372 701 3371 3660
Size: 372 329 2670 289
Fragments arranged by size:
2670 372 329 289
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 666 672 696 702 2866 2896 3154 3184
Size: 666 6 24 6 2164 30 258 30
Cuts at: 3184 3660
Size: 476
Fragments arranged by size:
2164 666 476 258 30 30 24 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 761 1093 1909 3660
Size: 761 332 816 1751
Fragments arranged by size:
1751 816 761 332
BseYI C'CCAG_C
Cuts at: 0 460 1475 1847 2478 2671 3531 3660
Size: 460 1015 372 631 193 860 129
Fragments arranged by size:
1015 860 631 460 372 193 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 865 1935 3061 3328 3660
Size: 242 623 1070 1126 267 332
Fragments arranged by size:
1126 1070 623 332 267 242
BsiHKAI G_wGCw'C
Cuts at: 0 887 899 1134 2090 3467 3660
Size: 887 12 235 956 1377 193
Fragments arranged by size:
1377 956 887 235 193 12
BsmI GAATG_Cn'
Cuts at: 0 1271 3660
Size: 1271 2389
BsmBI CGTCTCn'nnnn_
Cuts at: 0 265 3660
Size: 265 3395
Bsp1286I G_dGCh'C
Cuts at: 0 785 887 899 1134 1355 1381 2090 2898
Size: 785 102 12 235 221 26 709 808
Cuts at: 2898 3225 3420 3467 3660
Size: 327 195 47 193
Fragments arranged by size:
808 785 709 327 235 221 195 193
102 47 26 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3381 3660
Size: 3381 279
BsrBI CCG'CTC
Cuts at: 0 614 729 3660
Size: 614 115 2931
Fragments arranged by size:
2931 614 115
BsrDI GCAATG_nn'
Cuts at: 0 1433 3660
Size: 1433 2227
BsrGI T'GTAC_A
Cuts at: 0 2240 2705 2882 3143 3602 3660
Size: 2240 465 177 261 459 58
Fragments arranged by size:
2240 465 459 261 177 58
BssSI C'ACGA_G
Cuts at: 0 2620 3660
Size: 2620 1040
BstEII G'GTnAC_C
Cuts at: 0 3367 3660
Size: 3367 293
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1196 1860 3660
Size: 1196 664 1800
Fragments arranged by size:
1800 1196 664
BstYI r'GATC_y
Cuts at: 0 306 1032 1745 1771 2372 3660
Size: 306 726 713 26 601 1288
Fragments arranged by size:
1288 726 713 601 306 26
Bsu36I CC'TnA_GG
Cuts at: 0 1280 3660
Size: 1280 2380
BtgI C'CryG_G
Cuts at: 0 17 53 1505 2801 2870 3178 3660
Size: 17 36 1452 1296 69 308 482
Fragments arranged by size:
1452 1296 482 308 69 36 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2516 3660
Size: 2516 1144
BtsI GCAGTG_nn'
Cuts at: 0 2171 3009 3330 3660
Size: 2171 838 321 330
Fragments arranged by size:
2171 838 330 321
DraIII CAC_nnn'GTG
Cuts at: 0 2320 3660
Size: 2320 1340
DrdI GACnn_nn'nnGTC
Cuts at: 0 1665 3660
Size: 1665 1995
EaeI y'GGCC_r
Cuts at: 0 917 1445 1877 3660
Size: 917 528 432 1783
Fragments arranged by size:
1783 917 528 432
EarI CTCTTCn'nnn_
Cuts at: 0 358 773 1256 1325 1851 2450 3427 3660
Size: 358 415 483 69 526 599 977 233
Fragments arranged by size:
977 599 526 483 415 358 233 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1822 3660
Size: 1822 1838
EcoICRI GAG'CTC
Cuts at: 0 897 3660
Size: 897 2763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 364 758 1046 1157 1729 2974 3134
Size: 266 98 394 288 111 572 1245 160
Cuts at: 3134 3651 3660
Size: 517 9
Fragments arranged by size:
1245 572 517 394 288 266 160 111
98 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1807 3660
Size: 1807 1853
EcoO109I rG'GnC_Cy
Cuts at: 0 49 141 632 1305 2401 2894 2895 2910
Size: 49 92 491 673 1096 493 1 15
Cuts at: 2910 3660
Size: 750
Fragments arranged by size:
1096 750 673 493 491 92 49 15
1
EcoRV GAT'ATC
Cuts at: 0 664 2325 3660
Size: 664 1661 1335
Fragments arranged by size:
1661 1335 664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 369 1641 3660
Size: 369 1272 2019
Fragments arranged by size:
2019 1272 369
HaeII r_GCGC'y
Cuts at: 0 1170 3660
Size: 1170 2490
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 67 99 666 672 698 704
Size: 17 32 18 32 567 6 26 6
Cuts at: 704 1756 1788 2250 2282 2806 2838 3660
Size: 1052 32 462 32 524 32 822
Fragments arranged by size:
1052 822 567 524 462 32 32 32
32 32 26 18 17 6 6
HincII GTy'rAC
Cuts at: 0 1530 3660
Size: 1530 2130
HindIII A'AGCT_T
Cuts at: 0 449 3660
Size: 449 3211
MfeI C'AATT_G
Cuts at: 0 1717 3660
Size: 1717 1943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 380 2030 3197 3660
Size: 20 360 1650 1167 463
Fragments arranged by size:
1650 1167 463 360 20
MscI TGG'CCA
Cuts at: 0 919 1447 1879 3660
Size: 919 528 432 1781
Fragments arranged by size:
1781 919 528 432
MslI CAynn'nnrTG
Cuts at: 0 1372 2081 2083 2746 2869 2986 3660
Size: 1372 709 2 663 123 117 674
Fragments arranged by size:
1372 709 674 663 123 117 2
MspA1I CmG'CkG
Cuts at: 0 55 849 1479 2880 3660
Size: 55 794 630 1401 780
Fragments arranged by size:
1401 794 780 630 55
NcoI C'CATG_G
Cuts at: 0 17 1505 2801 2870 3178 3660
Size: 17 1488 1296 69 308 482
Fragments arranged by size:
1488 1296 482 308 69 17
NheI G'CTAG_C
Cuts at: 0 3135 3660
Size: 3135 525
NspI r_CATG'y
Cuts at: 0 760 1624 3486 3660
Size: 760 864 1862 174
Fragments arranged by size:
1862 864 760 174
PasI CC'CwG_GG
Cuts at: 0 2397 2686 3660
Size: 2397 289 974
Fragments arranged by size:
2397 974 289
PflMI CCAn_nnn'nTGG
Cuts at: 0 1803 1923 2622 3179 3393 3459 3629 3660
Size: 1803 120 699 557 214 66 170 31
Fragments arranged by size:
1803 699 557 214 170 120 66 31
PfoI T'CCnGG_A
Cuts at: 0 628 670 3151 3660
Size: 628 42 2481 509
Fragments arranged by size:
2481 628 509 42
PpuMI rG'GwC_Cy
Cuts at: 0 141 632 1305 2401 3660
Size: 141 491 673 1096 1259
Fragments arranged by size:
1259 1096 673 491 141
PsiI TTA'TAA
Cuts at: 0 803 3660
Size: 803 2857
PspOMI G'GGCC_C
Cuts at: 0 2894 3221 3660
Size: 2894 327 439
Fragments arranged by size:
2894 439 327
PspXI vC'TCGA_Gb
Cuts at: 0 73 3660
Size: 73 3587
PstI C_TGCA'G
Cuts at: 0 3004 3325 3660
Size: 3004 321 335
Fragments arranged by size:
3004 335 321
PvuII CAG'CTG
Cuts at: 0 849 2880 3660
Size: 849 2031 780
Fragments arranged by size:
2031 849 780
SacI G_AGCT'C
Cuts at: 0 899 3660
Size: 899 2761
SacII CC_GC'GG
Cuts at: 0 56 3660
Size: 56 3604
SapI GCTCTTCn'nnn_
Cuts at: 0 773 1325 2450 3660
Size: 773 552 1125 1210
Fragments arranged by size:
1210 1125 773 552
SexAI A'CCwGG_T
Cuts at: 0 2316 3660
Size: 2316 1344
SfcI C'TryA_G
Cuts at: 0 11 3000 3054 3321 3660
Size: 11 2989 54 267 339
Fragments arranged by size:
2989 339 267 54 11
SmaI CCC'GGG
Cuts at: 0 637 3660
Size: 637 3023
SmlI C'TyrA_G
Cuts at: 0 73 1309 1675 2794 3641 3660
Size: 73 1236 366 1119 847 19
Fragments arranged by size:
1236 1119 847 366 73 19
SphI G_CATG'C
Cuts at: 0 1624 3660
Size: 1624 2036
StuI AGG'CCT
Cuts at: 0 878 3660
Size: 878 2782
StyI C'CwwG_G
Cuts at: 0 17 1442 1505 2156 2801 2870 3062 3178
Size: 17 1425 63 651 645 69 192 116
Cuts at: 3178 3473 3660
Size: 295 187
Fragments arranged by size:
1425 651 645 295 192 187 116 69
63 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 656 1810 1829 3432 3660
Size: 656 1154 19 1603 228
Fragments arranged by size:
1603 1154 656 228 19
TatI w'GTAC_w
Cuts at: 0 1976 2240 2435 2705 2882 3143 3602 3660
Size: 1976 264 195 270 177 261 459 58
Fragments arranged by size:
1976 459 270 264 261 195 177 58
XbaI T'CTAG_A
Cuts at: 0 658 3660
Size: 658 3002
XhoI C'TCGA_G
Cuts at: 0 73 3641 3660
Size: 73 3568 19
Fragments arranged by size:
3568 73 19
XmaI C'CCGG_G
Cuts at: 0 635 3660
Size: 635 3025
XmnI GAAnn'nnTTC
Cuts at: 0 1244 3049 3660
Size: 1244 1805 611
Fragments arranged by size:
1805 1244 611
Enzymes that do cut:
AarI AcuI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AvaI BaeI BanII BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NheI
NspI PasI PflMI PfoI PpuMI PsiI PspOMI PspXI
PstI PvuII SacI SacII SapI SexAI SfcI SmaI
SmlI SphI StuI StyI TaqII TatI XbaI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AscI AseI AsiSI AvrII BamHI BanI BbeI
BfrBI BglI BmgBI BsaAI BsiEI BsiWI BspEI BspHI
BsrFI BssHII BstAPI BstBI BstZ17I ClaI CspCI DraI
EagI EcoRI FseI FspI FspAI HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XcmI ZraI