Restriction map for
pFN21AB0174
(Linear) (Six-base) MAPSORT of: KIBB0174.seq Check: 5827 from: 1 to: 4074
KIBB0174.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AatII G_ACGT'C
Cuts at: 0 1710 4074
Size: 1710 2364
AccI GT'mk_AC
Cuts at: 0 252 668 2072 4074
Size: 252 416 1404 2002
Fragments arranged by size:
2002 1404 416 252
Acc65I G'GTAC_C
Cuts at: 0 3487 4074
Size: 3487 587
AclI AA'CG_TT
Cuts at: 0 600 900 1003 4074
Size: 600 300 103 3071
Fragments arranged by size:
3071 600 300 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1325 2332 2815 3914 4074
Size: 955 370 1007 483 1099 160
Fragments arranged by size:
1099 1007 955 483 370 160
AfeI AGC'GCT
Cuts at: 0 376 4074
Size: 376 3698
AflIII A'CryG_T
Cuts at: 0 2541 3028 4074
Size: 2541 487 1046
Fragments arranged by size:
2541 1046 487
AgeI A'CCGG_T
Cuts at: 0 2763 4074
Size: 2763 1311
AhdI GACnn_n'nnGTC
Cuts at: 0 3378 4074
Size: 3378 696
AleI CACnn'nnGTG
Cuts at: 0 1689 4074
Size: 1689 2385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2882 2996 4074
Size: 2882 114 1078
Fragments arranged by size:
2882 1078 114
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3496 3528 4074
Size: 3496 32 546
Fragments arranged by size:
3496 546 32
AlwNI CAG_nnn'CTG
Cuts at: 0 396 2310 4074
Size: 396 1914 1764
Fragments arranged by size:
1914 1764 396
ApaI G_GGCC'C
Cuts at: 0 3961 4074
Size: 3961 113
ApaLI G'TGCA_C
Cuts at: 0 3683 4074
Size: 3683 391
ApoI r'AATT_y
Cuts at: 0 1885 1963 3263 3908 4074
Size: 1885 78 1300 645 166
Fragments arranged by size:
1885 1300 645 166 78
AvaI C'yCGr_G
Cuts at: 0 122 4074
Size: 122 3952
AvrII C'CTAG_G
Cuts at: 0 1291 4074
Size: 1291 2783
BamHI G'GATC_C
Cuts at: 0 174 2289 4074
Size: 174 2115 1785
Fragments arranged by size:
2115 1785 174
BanI G'GyrC_C
Cuts at: 0 2020 3487 4074
Size: 2020 1467 587
Fragments arranged by size:
2020 1467 587
BanII G_rGCy'C
Cuts at: 0 1589 3555 3961 4074
Size: 1589 1966 406 113
Fragments arranged by size:
1966 1589 406 113
BbsI GAAGACnn'nnnn_
Cuts at: 0 536 2357 3088 3374 4074
Size: 536 1821 731 286 700
Fragments arranged by size:
1821 731 700 536 286
BbvCI CC'TCA_GC
Cuts at: 0 1880 4074
Size: 1880 2194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2665 2699 4074
Size: 2665 34 1375
Fragments arranged by size:
2665 1375 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2003 4074
Size: 2003 2071
BclI T'GATC_A
Cuts at: 0 2637 4074
Size: 2637 1437
BfrBI ATG'CAT
Cuts at: 0 3303 4074
Size: 3303 771
BglII A'GATC_T
Cuts at: 0 3097 4074
Size: 3097 977
BlpI GC'TnA_GC
Cuts at: 0 746 2882 4074
Size: 746 2136 1192
Fragments arranged by size:
2136 1192 746
Bme1580I G_kGCm'C
Cuts at: 0 3687 3961 4074
Size: 3687 274 113
Fragments arranged by size:
3687 274 113
BmrI ACTGGGnnnn_n'
Cuts at: 0 981 2489 2666 4074
Size: 981 1508 177 1408
Fragments arranged by size:
1508 1408 981 177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 343 4074
Size: 343 3731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 446 3338 4074
Size: 232 214 2892 736
Fragments arranged by size:
2892 736 232 214
Bpu10I CC'TnA_GC
Cuts at: 0 1880 4074
Size: 1880 2194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1332 1831 4074
Size: 1332 499 2243
Fragments arranged by size:
2243 1332 499
BsaAI yAC'GTr
Cuts at: 0 1780 4074
Size: 1780 2294
BsaBI GATnn'nnATC
Cuts at: 0 1772 3302 3755 4074
Size: 1772 1530 453 319
Fragments arranged by size:
1772 1530 453 319
BsaHI Gr'CG_yC
Cuts at: 0 1707 2937 3564 4074
Size: 1707 1230 627 510
Fragments arranged by size:
1707 1230 627 510
BsaWI w'CCGG_w
Cuts at: 0 1377 2763 4074
Size: 1377 1386 1311
Fragments arranged by size:
1386 1377 1311
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3116 3146 4074
Size: 3116 30 928
Fragments arranged by size:
3116 928 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 588 710 1827 2657 3704 4074
Size: 588 122 1117 830 1047 370
Fragments arranged by size:
1117 1047 830 588 370 122
BseYI C'CCAG_C
Cuts at: 0 3368 4074
Size: 3368 706
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 4074
Size: 224 3850
BsiHKAI G_wGCw'C
Cuts at: 0 1589 3324 3687 4074
Size: 1589 1735 363 387
Fragments arranged by size:
1735 1589 387 363
Bsp1286I G_dGCh'C
Cuts at: 0 1589 3324 3555 3687 3961 4074
Size: 1589 1735 231 132 274 113
Fragments arranged by size:
1735 1589 274 231 132 113
BspEI T'CCGG_A
Cuts at: 0 1377 4074
Size: 1377 2697
BspHI T'CATG_A
Cuts at: 0 69 2378 2430 2967 3984 4074
Size: 69 2309 52 537 1017 90
Fragments arranged by size:
2309 1017 537 90 69 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 4074
Size: 721 3353
BsrDI GCAATG_nn'
Cuts at: 0 20 181 719 945 1284 2871 3396 4074
Size: 20 161 538 226 339 1587 525 678
Fragments arranged by size:
1587 678 538 525 339 226 161 20
BsrFI r'CCGG_y
Cuts at: 0 2763 4074
Size: 2763 1311
BstAPI GCAn_nnn'nTGC
Cuts at: 0 315 4074
Size: 315 3759
BstBI TT'CG_AA
Cuts at: 0 2805 4074
Size: 2805 1269
BstEII G'GTnAC_C
Cuts at: 0 465 4074
Size: 465 3609
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2412 2933 3205 4074
Size: 2412 521 272 869
Fragments arranged by size:
2412 869 521 272
BstYI r'GATC_y
Cuts at: 0 35 174 392 648 2289 3097 4074
Size: 35 139 218 256 1641 808 977
Fragments arranged by size:
1641 977 808 256 218 139 35
Bsu36I CC'TnA_GG
Cuts at: 0 1109 3376 4074
Size: 1109 2267 698
Fragments arranged by size:
2267 1109 698
BtgI C'CryG_G
Cuts at: 0 2024 3491 3726 3974 4074
Size: 2024 1467 235 248 100
Fragments arranged by size:
2024 1467 248 235 100
BtsI GCAGTG_nn'
Cuts at: 0 2866 3489 3687 4074
Size: 2866 623 198 387
Fragments arranged by size:
2866 623 387 198
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1053 1088 4074
Size: 1053 35 2986
Fragments arranged by size:
2986 1053 35
DraI TTT'AAA
Cuts at: 0 114 2159 3404 3416 4074
Size: 114 2045 1245 12 658
Fragments arranged by size:
2045 1245 658 114 12
EarI CTCTTCn'nnn_
Cuts at: 0 700 2270 2339 4074
Size: 700 1570 69 1735
Fragments arranged by size:
1735 1570 700 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2270 4074
Size: 2270 1804
EcoICRI GAG'CTC
Cuts at: 0 1587 4074
Size: 1587 2487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 446 955 1325 2332 2815 3338 3914
Size: 232 214 509 370 1007 483 523 576
Cuts at: 3914 4074
Size: 160
Fragments arranged by size:
1007 576 523 509 483 370 232 214
160
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3870 4074
Size: 3870 204
EcoO109I rG'GnC_Cy
Cuts at: 0 2619 3372 3824 3957 4074
Size: 2619 753 452 133 117
Fragments arranged by size:
2619 753 452 133 117
EcoRI G'AATT_C
Cuts at: 0 3263 4074
Size: 3263 811
EcoRV GAT'ATC
Cuts at: 0 1365 4074
Size: 1365 2709
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2374 2512 4074
Size: 2374 138 1562
Fragments arranged by size:
2374 1562 138
HaeII r_GCGC'y
Cuts at: 0 378 4074
Size: 378 3696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2076 2108 2962 2994 2998 3030 3116 3148
Size: 2076 32 854 32 4 32 86 32
Cuts at: 3148 3364 3396 3403 3435 3653 3685 4074
Size: 216 32 7 32 218 32 389
Fragments arranged by size:
2076 854 389 218 216 86 32 32
32 32 32 32 32 7 4
HincII GTy'rAC
Cuts at: 0 1322 2708 4074
Size: 1322 1386 1366
Fragments arranged by size:
1386 1366 1322
KpnI G_GTAC'C
Cuts at: 0 3491 4074
Size: 3491 583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 4074
Size: 454 3620
MslI CAynn'nnrTG
Cuts at: 0 1565 1689 1778 2429 2931 2966 4074
Size: 1565 124 89 651 502 35 1108
Fragments arranged by size:
1565 1108 651 502 124 89 35
MspA1I CmG'CkG
Cuts at: 0 403 2065 3607 3775 4074
Size: 403 1662 1542 168 299
Fragments arranged by size:
1662 1542 403 299 168
NcoI C'CATG_G
Cuts at: 0 3491 3726 4074
Size: 3491 235 348
Fragments arranged by size:
3491 348 235
NsiI A_TGCA'T
Cuts at: 0 3305 4074
Size: 3305 769
NspI r_CATG'y
Cuts at: 0 2241 2545 2586 2613 3032 4074
Size: 2241 304 41 27 419 1042
Fragments arranged by size:
2241 1042 419 304 41 27
PciI A'CATG_T
Cuts at: 0 2541 3028 4074
Size: 2541 487 1046
Fragments arranged by size:
2541 1046 487
PflMI CCAn_nnn'nTGG
Cuts at: 0 2006 2929 4074
Size: 2006 923 1145
Fragments arranged by size:
2006 1145 923
PfoI T'CCnGG_A
Cuts at: 0 1487 3902 4074
Size: 1487 2415 172
Fragments arranged by size:
2415 1487 172
PpuMI rG'GwC_Cy
Cuts at: 0 3372 3824 4074
Size: 3372 452 250
Fragments arranged by size:
3372 452 250
PshAI GACnn'nnGTC
Cuts at: 0 2737 4074
Size: 2737 1337
PsiI TTA'TAA
Cuts at: 0 1144 4074
Size: 1144 2930
PspOMI G'GGCC_C
Cuts at: 0 3957 4074
Size: 3957 117
PspXI vC'TCGA_Gb
Cuts at: 0 122 4074
Size: 122 3952
PstI C_TGCA'G
Cuts at: 0 1017 3750 4074
Size: 1017 2733 324
Fragments arranged by size:
2733 1017 324
PvuII CAG'CTG
Cuts at: 0 403 2065 3607 3775 4074
Size: 403 1662 1542 168 299
Fragments arranged by size:
1662 1542 403 299 168
SacI G_AGCT'C
Cuts at: 0 1589 4074
Size: 1589 2485
ScaI AGT'ACT
Cuts at: 0 102 3846 4074
Size: 102 3744 228
Fragments arranged by size:
3744 228 102
SfcI C'TryA_G
Cuts at: 0 44 1013 1156 3738 3746 3870 4074
Size: 44 969 143 2582 8 124 204
Fragments arranged by size:
2582 969 204 143 124 44 8
SmlI C'TyrA_G
Cuts at: 0 122 1311 1846 4074
Size: 122 1189 535 2228
Fragments arranged by size:
2228 1189 535 122
SpeI A'CTAG_T
Cuts at: 0 3847 4074
Size: 3847 227
SspI AAT'ATT
Cuts at: 0 956 1371 1650 4074
Size: 956 415 279 2424
Fragments arranged by size:
2424 956 415 279
StuI AGG'CCT
Cuts at: 0 143 4074
Size: 143 3931
StyI C'CwwG_G
Cuts at: 0 995 1291 3153 3491 3726 4074
Size: 995 296 1862 338 235 348
Fragments arranged by size:
1862 995 348 338 296 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3585 4074
Size: 3585 489
TatI w'GTAC_w
Cuts at: 0 100 1497 2059 2776 3844 4074
Size: 100 1397 562 717 1068 230
Fragments arranged by size:
1397 1068 717 562 230 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 507 539 4074
Size: 507 32 3535
Fragments arranged by size:
3535 507 32
XbaI T'CTAG_A
Cuts at: 0 3267 4045 4074
Size: 3267 778 29
Fragments arranged by size:
3267 778 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2930 3639 4074
Size: 2930 709 435
Fragments arranged by size:
2930 709 435
XhoI C'TCGA_G
Cuts at: 0 122 4074
Size: 122 3952
XmnI GAAnn'nnTTC
Cuts at: 0 1004 1202 1385 2259 2374 4074
Size: 1004 198 183 874 115 1700
Fragments arranged by size:
1700 1004 874 198 183 115
ZraI GAC'GTC
Cuts at: 0 1708 4074
Size: 1708 2366
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI DraI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
KpnI MmeI MslI MspA1I NcoI NsiI NspI PciI
PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuII SacI ScaI SfcI SmlI SpeI SspI StuI
StyI TaqII TatI TstI XbaI XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AscI AseI AsiSI BaeI BbeI BglI
BmgBI BmtI BsaI BsiEI BsiWI BsmI BsmBI BsrBI
BsrGI BssHII BssSI BstZ17I BtgZI ClaI DraIII DrdI
EaeI EagI FseI FspI FspAI HindIII HpaI KasI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PmeI PmlI PpiI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SwaI Tth111I XmaI