(Circular) (Six-base) MAPSORT of: pFN21AB0166.seq Check: 6877 from: 1 to: 8173 pFN21AB0166 8173 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 5983 278 Size: 53 83 186 936 2338 2109 2468 Fragments arranged by size: 2468 2338 2109 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3702 5516 1937 Size: 1765 1814 4594 Fragments arranged by size: 4594 1814 1765 Acc65I G'GTAC_C Cuts at: 2039 5494 2039 Size: 3455 4718 AclI AA'CG_TT Cuts at: 1563 2252 6300 6673 1563 Size: 689 4048 373 3063 Fragments arranged by size: 4048 3063 689 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2322 2414 2895 2972 3222 3465 4191 4382 Size: 690 92 481 77 250 243 726 191 Cuts at: 4382 4929 5222 6236 7620 7888 1632 Size: 547 293 1014 1384 268 1917 Fragments arranged by size: 1917 1384 1014 726 690 547 481 293 268 250 243 191 92 77 AfeI AGC'GCT Cuts at: 4664 5003 4664 Size: 339 7834 AflII C'TTAA_G Cuts at: 820 1017 4463 820 Size: 197 3446 4530 Fragments arranged by size: 4530 3446 197 AflIII A'CryG_T Cuts at: 1203 7072 8049 1203 Size: 5869 977 1327 Fragments arranged by size: 5869 1327 977 AgeI A'CCGG_T Cuts at: 7004 7004 Size: 8173 AhdI GACnn_n'nnGTC Cuts at: 6902 6902 Size: 8173 AleI CACnn'nnGTG Cuts at: 1166 2457 1166 Size: 1291 6882 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8173 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3959 3991 3959 Size: 32 8141 AlwNI CAG_nnn'CTG Cuts at: 3164 3299 3488 3728 4573 5041 5916 7488 3164 Size: 135 189 240 845 468 875 1572 3849 Fragments arranged by size: 3849 1572 875 845 468 240 189 135 ApaI G_GGCC'C Cuts at: 2060 2079 2060 Size: 19 8154 ApaLI G'TGCA_C Cuts at: 6230 7386 6230 Size: 1156 7017 ApoI r'AATT_y Cuts at: 1493 2796 3305 4690 4783 5482 5708 5860 7969 Size: 1303 509 1385 93 699 226 152 2109 Cuts at: 7969 1493 Size: 1697 Fragments arranged by size: 2109 1697 1385 1303 699 509 226 152 93 AseI AT'TA_AT Cuts at: 160 1032 4827 6727 160 Size: 872 3795 1900 1606 Fragments arranged by size: 3795 1900 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8173 AvaI C'yCGr_G Cuts at: 1870 1943 2035 2072 5498 1870 Size: 73 92 37 3426 4545 Fragments arranged by size: 4545 3426 92 73 37 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4613 4646 4613 Size: 33 8140 BamHI G'GATC_C Cuts at: 5503 5870 5503 Size: 367 7806 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 3368 5494 6949 618 Size: 325 217 627 252 1329 2126 1455 1842 Fragments arranged by size: 2126 1842 1455 1329 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 2060 2079 2714 2950 3636 5492 1391 Size: 669 19 635 236 686 1856 4072 Fragments arranged by size: 4072 1856 686 669 635 236 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2664 3210 3462 3816 928 Size: 971 765 546 252 354 5285 Fragments arranged by size: 5285 971 765 546 354 252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6364 6398 1903 Size: 34 4427 34 3678 Fragments arranged by size: 4427 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4093 6065 7275 4093 Size: 1972 1210 4991 Fragments arranged by size: 4991 1972 1210 BclI T'GATC_A Cuts at: 1548 4179 4684 1548 Size: 2631 505 5037 Fragments arranged by size: 5037 2631 505 BfrBI ATG'CAT Cuts at: 2924 2924 Size: 8173 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2746 6784 136 Size: 107 122 71 631 605 1074 4038 1525 Fragments arranged by size: 4038 1525 1074 631 605 122 107 71 BglII A'GATC_T Cuts at: 4537 5313 8168 4537 Size: 776 2855 4542 Fragments arranged by size: 4542 2855 776 BlpI GC'TnA_GC Cuts at: 2177 4992 5590 2177 Size: 2815 598 4760 Fragments arranged by size: 4760 2815 598 Bme1580I G_kGCm'C Cuts at: 1790 2060 2079 6234 7390 1790 Size: 270 19 4155 1156 2573 Fragments arranged by size: 4155 2573 1156 270 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8173 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2135 3309 4669 Size: 416 53 83 399 24 718 1174 1360 Cuts at: 4669 5904 6862 442 Size: 1235 958 1753 Fragments arranged by size: 1753 1360 1235 1174 958 718 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2103 1056 Size: 1047 7126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2476 4136 4529 4643 4742 6833 1239 Size: 1237 1660 393 114 99 2091 2579 Fragments arranged by size: 2579 2091 1660 1237 393 114 99 Bpu10I CC'TnA_GC Cuts at: 2713 2713 Size: 8173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3947 4156 4184 4942 6298 7163 7461 3947 Size: 209 28 758 1356 865 298 4659 Fragments arranged by size: 4659 1356 865 758 298 209 28 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3512 5015 6836 882 Size: 617 2013 1503 1821 2219 Fragments arranged by size: 2219 2013 1821 1503 617 BsaAI yAC'GTr Cuts at: 493 1204 5964 493 Size: 711 4760 2702 Fragments arranged by size: 4760 2702 711 BsaBI GATnn'nnATC Cuts at: 8167 8167 Size: 8173 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3871 5980 6362 6997 Size: 53 83 186 936 2338 2109 382 635 Cuts at: 6997 275 Size: 1451 Fragments arranged by size: 2338 2109 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5362 6606 7004 7278 7425 7836 5362 Size: 1244 398 274 147 411 5699 Fragments arranged by size: 5699 1244 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3339 3369 3959 3989 1178 Size: 30 165 30 1936 30 590 30 5362 Fragments arranged by size: 5362 1936 590 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4139 4539 1186 Size: 2953 400 4820 Fragments arranged by size: 4820 2953 400 BseYI C'CCAG_C Cuts at: 2011 3721 7376 7872 2011 Size: 1710 3655 496 2312 Fragments arranged by size: 3655 2312 1710 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2669 5070 1163 Size: 573 933 2401 4266 Fragments arranged by size: 4266 2401 933 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2160 2164 5623 6384 7412 1540 Size: 275 186 159 4 3459 761 1028 2301 Fragments arranged by size: 3459 2301 1028 761 275 186 159 4 BsiHKAI G_wGCw'C Cuts at: 2148 2714 3290 3636 6234 6319 7390 2148 Size: 566 576 346 2598 85 1071 2931 Fragments arranged by size: 2931 2598 1071 576 566 346 85 BsmI GAATG_Cn' Cuts at: 5340 5684 5777 5340 Size: 344 93 7736 Fragments arranged by size: 7736 344 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2079 2148 2714 2950 3290 3636 Size: 399 270 19 69 566 236 340 346 Cuts at: 3636 5492 6234 6319 7390 1391 Size: 1856 742 85 1071 2174 Fragments arranged by size: 2174 1856 1071 742 566 399 346 340 270 236 85 69 19 BspEI T'CCGG_A Cuts at: 7836 7836 Size: 8173 BspHI T'CATG_A Cuts at: 4798 6062 7792 4798 Size: 1264 1730 5179 Fragments arranged by size: 5179 1730 1264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5385 5528 844 Size: 4541 143 3489 Fragments arranged by size: 4541 3489 143 BsrBI CCG'CTC Cuts at: 1394 1823 6060 1394 Size: 429 4237 3507 Fragments arranged by size: 4237 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6668 6842 65 Size: 1183 5420 174 1396 Fragments arranged by size: 5420 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2155 2163 6817 7004 1700 Size: 110 345 8 4654 187 2869 Fragments arranged by size: 4654 2869 345 187 110 8 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8173 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8173 BssSI C'ACGA_G Cuts at: 6233 7245 6233 Size: 1012 7161 BstAPI GCAn_nnn'nTGC Cuts at: 794 2737 2755 794 Size: 1943 18 6212 Fragments arranged by size: 6212 1943 18 BstBI TT'CG_AA Cuts at: 6977 6977 Size: 8173 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8173 BstYI r'GATC_y Cuts at: 1972 2258 2549 2981 4537 5313 5503 5870 6255 Size: 286 291 432 1556 776 190 367 385 Cuts at: 6255 6272 7724 7810 7822 8168 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1556 1452 776 432 385 367 346 291 286 190 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2494 2742 3756 2494 Size: 248 1014 6911 Fragments arranged by size: 6911 1014 248 BtgI C'CryG_G Cuts at: 513 1065 2027 3734 4295 513 Size: 552 962 1707 561 4391 Fragments arranged by size: 4391 1707 962 561 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5084 487 Size: 683 56 3858 3576 Fragments arranged by size: 3858 3576 683 56 BtsI GCAGTG_nn' Cuts at: 2652 5689 6483 6503 2652 Size: 3037 794 20 4322 Fragments arranged by size: 4322 3037 794 20 ClaI AT'CG_AT Cuts at: 1554 4431 1554 Size: 2877 5296 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8138 DraI TTT'AAA Cuts at: 5331 5477 5331 Size: 146 8027 DraIII CAC_nnn'GTG Cuts at: 2444 2444 Size: 8173 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7180 809 Size: 507 5864 1802 Fragments arranged by size: 5864 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2161 2564 Size: 54 1425 50 275 18 234 97 403 Cuts at: 2564 3906 5620 6509 8138 8 Size: 1342 1714 889 1629 43 Fragments arranged by size: 1714 1629 1425 1342 889 403 275 234 97 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2161 5620 1537 Size: 275 349 3459 4090 Fragments arranged by size: 4090 3459 349 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2271 2491 3318 4252 4557 4712 5122 Size: 800 118 220 827 934 305 155 410 Cuts at: 5122 5241 6103 1353 Size: 119 862 3423 Fragments arranged by size: 3423 934 862 827 800 410 305 220 155 119 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5060 6760 7134 7280 387 Size: 1064 138 3471 1700 374 146 1280 Fragments arranged by size: 3471 1700 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2712 3634 2712 Size: 922 7251 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2322 2414 2476 2895 2972 3222 3465 Size: 393 690 92 62 419 77 250 243 Cuts at: 3465 4136 4191 4382 4529 4643 4742 4929 5222 Size: 671 55 191 147 114 99 187 293 Cuts at: 5222 6236 6833 7620 7888 1239 Size: 1014 597 787 268 1524 Fragments arranged by size: 1524 1014 787 690 671 597 419 393 293 268 250 243 191 187 147 114 99 92 77 62 55 EcoNI CCTnn'n_nnAGG Cuts at: 2741 2741 Size: 8173 EcoO109I rG'GnC_Cy Cuts at: 2046 2056 2046 Size: 10 8163 EcoRI G'AATT_C Cuts at: 5482 5860 5482 Size: 378 7795 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2774 2774 Size: 8173 FspI TGC'GCA Cuts at: 6679 6679 Size: 8173 HaeII r_GCGC'y Cuts at: 1678 4666 5005 7320 1678 Size: 2988 339 2315 2531 Fragments arranged by size: 2988 2531 2315 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3202 3234 3241 3273 3808 Size: 32 135 32 1630 32 7 32 535 Cuts at: 3808 3840 3895 3906 3927 3938 4524 4529 4556 Size: 32 55 11 21 11 586 5 27 Cuts at: 4556 4561 5237 5269 6814 6846 6888 6920 1373 Size: 5 676 32 1545 32 42 32 2626 Fragments arranged by size: 2626 1630 1545 676 586 535 135 55 42 32 32 32 32 32 32 32 32 27 21 11 11 7 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3019 3225 3997 5517 5763 669 Size: 988 281 1081 206 772 1520 246 3079 Fragments arranged by size: 3079 1520 1081 988 772 281 246 206 HindIII A'AGCT_T Cuts at: 748 2857 5154 5401 748 Size: 2109 2297 247 3520 Fragments arranged by size: 3520 2297 2109 247 HpaI GTT'AAC Cuts at: 5763 5763 Size: 8173 KpnI G_GTAC'C Cuts at: 2043 5498 2043 Size: 3455 4718 MfeI C'AATT_G Cuts at: 5280 5772 5280 Size: 492 7681 MluI A'CGCG_T Cuts at: 8049 8049 Size: 8173 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4071 5148 7287 7471 1305 Size: 2766 1077 2139 184 2007 Fragments arranged by size: 2766 2139 2007 1077 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2566 3908 10 Size: 54 1425 343 734 1342 4275 Fragments arranged by size: 4275 1425 1342 734 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2457 6131 6490 6649 518 Size: 648 1291 3674 359 159 2042 Fragments arranged by size: 3674 2042 1291 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2062 2317 2434 4417 4812 5038 5589 Size: 423 33 255 117 1983 395 226 551 Cuts at: 5589 6266 7414 7659 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1983 1148 677 551 423 395 255 245 226 117 33 NaeI GCC'GGC Cuts at: 2165 2165 Size: 8173 NcoI C'CATG_G Cuts at: 513 1065 4295 513 Size: 552 3230 4391 Fragments arranged by size: 4391 3230 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8173 NgoMIV G'CCGG_C Cuts at: 2163 2163 Size: 8173 NheI G'CTAG_C Cuts at: 1052 2099 1052 Size: 1047 7126 NotI GC'GGCC_GC Cuts at: 5620 5620 Size: 8173 NsiI A_TGCA'T Cuts at: 2926 2926 Size: 8173 NspI r_CATG'y Cuts at: 1132 1229 2758 2924 3935 5531 7076 1132 Size: 97 1529 166 1011 1596 1545 2229 Fragments arranged by size: 2229 1596 1545 1529 1011 166 97 PasI CC'CwG_GG Cuts at: 1348 2119 1348 Size: 771 7402 PciI A'CATG_T Cuts at: 7072 7072 Size: 8173 PflMI CCAn_nnn'nTGG Cuts at: 1108 4164 1108 Size: 3056 5117 PfoI T'CCnGG_A Cuts at: 4440 4440 Size: 8173 PmeI GTTT'AAAC Cuts at: 5477 5477 Size: 8173 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 6242 6274 7755 7787 3959 Size: 32 2251 32 1481 32 4345 Fragments arranged by size: 4345 2251 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2046 2046 Size: 8173 PshAI GACnn'nnGTC Cuts at: 1613 3415 1613 Size: 1802 6371 PsiI TTA'TAA Cuts at: 5743 5743 Size: 8173 PspOMI G'GGCC_C Cuts at: 2056 2075 2056 Size: 19 8154 PstI C_TGCA'G Cuts at: 830 2131 3596 4063 4215 5525 830 Size: 1301 1465 467 152 1310 3478 Fragments arranged by size: 3478 1465 1310 1301 467 152 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8173 PvuII CAG'CTG Cuts at: 2317 2434 4417 4812 5038 2317 Size: 117 1983 395 226 5452 Fragments arranged by size: 5452 1983 395 226 117 SacI G_AGCT'C Cuts at: 2714 3636 2714 Size: 922 7251 SacII CC_GC'GG Cuts at: 2030 2030 Size: 8173 SalI G'TCGA_C Cuts at: 1936 5515 1936 Size: 3579 4594 SanDI GG'GwC_CC Cuts at: 2046 2046 Size: 8173 SapI GCTCTTCn'nnn_ Cuts at: 2153 5122 5241 2153 Size: 2969 119 5085 Fragments arranged by size: 5085 2969 119 SbfI CC_TGCA'GG Cuts at: 5525 5525 Size: 8173 ScaI AGT'ACT Cuts at: 5357 6421 8133 5357 Size: 1064 1712 5397 Fragments arranged by size: 5397 1712 1064 SfcI C'TryA_G Cuts at: 826 1045 2127 3165 3540 3592 4059 4211 4523 Size: 219 1082 1038 375 52 467 152 312 Cuts at: 4523 5521 6656 7337 7528 826 Size: 998 1135 681 191 1471 Fragments arranged by size: 1471 1135 1082 1038 998 681 467 375 312 219 191 152 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8173 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8173 SmaI CCC'GGG Cuts at: 1872 2037 5500 1872 Size: 165 3463 4545 Fragments arranged by size: 4545 3463 165 SmlI C'TyrA_G Cuts at: 820 1017 1943 3962 4135 4199 4463 4921 6277 Size: 197 926 2019 173 64 264 458 1356 Cuts at: 6277 7178 7440 820 Size: 901 262 1553 Fragments arranged by size: 2019 1553 1356 926 901 458 264 262 197 173 64 SnaBI TAC'GTA Cuts at: 493 5964 493 Size: 5471 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8173 SphI G_CATG'C Cuts at: 1132 2758 2924 3935 5531 1132 Size: 1626 166 1011 1596 3774 Fragments arranged by size: 3774 1626 1596 1011 166 SspI AAT'ATT Cuts at: 5 52 4195 5453 6097 5 Size: 47 4143 1258 644 2081 Fragments arranged by size: 4143 2081 1258 644 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8173 StyI C'CwwG_G Cuts at: 513 1065 4295 5612 513 Size: 552 3230 1317 3074 Fragments arranged by size: 3230 3074 1317 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4424 6200 6217 6370 6555 7000 325 Size: 1255 2844 1776 17 153 185 445 1498 Fragments arranged by size: 2844 1776 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5355 6419 8131 Size: 274 80 33 51 1443 3378 1064 1712 Cuts at: 8131 96 Size: 138 Fragments arranged by size: 3378 1712 1443 1064 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2190 2222 3777 3809 8133 8165 2190 Size: 32 1555 32 4324 32 2198 Fragments arranged by size: 4324 2198 1555 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3992 1316 Size: 2676 5497 XbaI T'CTAG_A Cuts at: 3398 5509 3398 Size: 2111 6062 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8173 XmaI C'CCGG_G Cuts at: 1870 2035 5498 1870 Size: 165 3463 4545 Fragments arranged by size: 4545 3463 165 XmnI GAAnn'nnTTC Cuts at: 2779 4290 6302 2779 Size: 1511 2012 4650 Fragments arranged by size: 4650 2012 1511 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 5981 276 Size: 53 83 186 936 2338 2109 2468 Fragments arranged by size: 2468 2338 2109 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI XcmI