(Circular) (Six-base) MAPSORT of: pFN21AB0049.seq Check: 565 from: 1 to: 10174 pFN21AB0049 10174 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7984 278 Size: 53 83 186 936 6448 2468 Fragments arranged by size: 6448 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4719 6176 7517 1937 Size: 2782 1457 1341 4594 Fragments arranged by size: 4594 2782 1457 1341 Acc65I G'GTAC_C Cuts at: 7495 7495 Size: 10174 AclI AA'CG_TT Cuts at: 1563 2671 4434 8301 8674 1563 Size: 1108 1763 3867 373 3063 Fragments arranged by size: 3867 3063 1763 1108 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4780 5581 5817 6644 6771 7272 7402 8237 Size: 3148 801 236 827 127 501 130 835 Cuts at: 8237 9621 9889 1632 Size: 1384 268 1917 Fragments arranged by size: 3148 1917 1384 835 827 801 501 268 236 130 127 AfeI AGC'GCT Cuts at: 2059 2868 2059 Size: 809 9365 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9977 AflIII A'CryG_T Cuts at: 1203 3597 5326 5844 6597 9073 10050 1203 Size: 2394 1729 518 753 2476 977 1327 Fragments arranged by size: 2476 2394 1729 1327 977 753 518 AgeI A'CCGG_T Cuts at: 9005 9005 Size: 10174 AhdI GACnn_n'nnGTC Cuts at: 8903 8903 Size: 10174 AleI CACnn'nnGTG Cuts at: 1166 4334 5099 1166 Size: 3168 765 6241 Fragments arranged by size: 6241 3168 765 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2455 3842 4275 1184 Size: 1271 1387 433 7083 Fragments arranged by size: 7083 1387 1271 433 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3096 3128 5886 5918 6963 6995 3096 Size: 32 2758 32 1045 32 6275 Fragments arranged by size: 6275 2758 1045 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3391 4079 4195 4390 4782 7917 9489 3391 Size: 688 116 195 392 3135 1572 4076 Fragments arranged by size: 4076 3135 1572 688 392 195 116 ApaI G_GGCC'C Cuts at: 5566 5566 Size: 10174 ApaLI G'TGCA_C Cuts at: 2300 4541 8231 9387 2300 Size: 2241 3690 1156 3087 Fragments arranged by size: 3690 3087 2241 1156 ApoI r'AATT_y Cuts at: 1493 2578 3929 3954 4898 5207 6256 7483 7709 Size: 1085 1351 25 944 309 1049 1227 226 Cuts at: 7709 7861 9970 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1351 1227 1085 1049 944 309 226 152 25 AseI AT'TA_AT Cuts at: 160 1032 5397 8728 160 Size: 872 4365 3331 1606 Fragments arranged by size: 4365 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10174 AvaI C'yCGr_G Cuts at: 1870 1943 4546 4852 5112 7499 1870 Size: 73 2603 306 260 2387 4545 Fragments arranged by size: 4545 2603 2387 306 260 73 BamHI G'GATC_C Cuts at: 4455 6322 7504 7871 4455 Size: 1867 1182 367 6758 Fragments arranged by size: 6758 1867 1182 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6473 7442 7495 8950 618 Size: 325 217 627 4686 969 53 1455 1842 Fragments arranged by size: 4686 1842 1455 969 627 325 217 53 BanII G_rGCy'C Cuts at: 1391 2018 2123 3330 4108 4467 5539 5566 7493 Size: 627 105 1207 778 359 1072 27 1927 Cuts at: 7493 1391 Size: 4072 Fragments arranged by size: 4072 1927 1207 1072 778 627 359 105 27 BbeI G_GCGC'C Cuts at: 7446 7446 Size: 10174 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3519 5697 6213 6731 928 Size: 971 1620 2178 516 518 4371 Fragments arranged by size: 4371 2178 1620 971 518 516 BbvCI CC'TCA_GC Cuts at: 3749 4383 3749 Size: 634 9540 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2468 2502 4228 4262 8365 8399 1903 Size: 34 531 34 1726 34 4103 34 3678 Fragments arranged by size: 4103 3678 1726 531 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2285 2917 6965 8066 9276 2285 Size: 632 4048 1101 1210 3183 Fragments arranged by size: 4048 3183 1210 1101 632 BclI T'GATC_A Cuts at: 1548 4212 5049 7294 1548 Size: 2664 837 2245 4428 Fragments arranged by size: 4428 2664 2245 837 BfrBI ATG'CAT Cuts at: 3872 5607 3872 Size: 1735 8439 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2044 4268 8785 Size: 107 122 71 631 605 372 2224 4517 Cuts at: 8785 136 Size: 1525 Fragments arranged by size: 4517 2224 1525 631 605 372 122 107 71 BglII A'GATC_T Cuts at: 3463 5418 10169 3463 Size: 1955 4751 3468 Fragments arranged by size: 4751 3468 1955 BlpI GC'TnA_GC Cuts at: 2017 2122 2900 7591 2017 Size: 105 778 4691 4600 Fragments arranged by size: 4691 4600 778 105 Bme1580I G_kGCm'C Cuts at: 1790 2247 2304 2402 4126 4545 5113 5566 8235 Size: 457 57 98 1724 419 568 453 2669 Cuts at: 8235 9391 1790 Size: 1156 2573 Fragments arranged by size: 2669 2573 1724 1156 568 457 453 419 98 57 BmgBI CAC'GTC Cuts at: 1318 4581 5845 1318 Size: 3263 1264 5647 Fragments arranged by size: 5647 3263 1264 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7403 7905 8863 Size: 416 53 83 399 24 5986 502 958 Cuts at: 8863 442 Size: 1753 Fragments arranged by size: 5986 1753 958 502 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2074 3079 3449 3452 5526 5669 6851 8834 Size: 835 1005 370 3 2074 143 1182 1983 Cuts at: 8834 1239 Size: 2579 Fragments arranged by size: 2579 2074 1983 1182 1005 835 370 143 3 Bpu10I CC'TnA_GC Cuts at: 2287 3749 4383 2287 Size: 1462 634 8078 Fragments arranged by size: 8078 1462 634 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 8299 9164 9462 8299 Size: 865 298 9011 Fragments arranged by size: 9011 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3395 6722 8837 882 Size: 617 1896 3327 2115 2219 Fragments arranged by size: 3327 2219 2115 1896 617 BsaAI yAC'GTr Cuts at: 493 1204 2935 6976 7965 493 Size: 711 1731 4041 989 2702 Fragments arranged by size: 4041 2702 1731 989 711 BsaBI GATnn'nnATC Cuts at: 3017 5423 10168 3017 Size: 2406 4745 3023 Fragments arranged by size: 4745 3023 2406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2179 2697 7443 7981 Size: 53 83 186 936 646 518 4746 538 Cuts at: 7981 8363 8998 275 Size: 382 635 1451 Fragments arranged by size: 4746 1451 936 646 635 538 518 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2654 2759 5168 7049 8607 9005 9279 9426 9837 Size: 105 2409 1881 1558 398 274 147 411 Cuts at: 9837 2654 Size: 2991 Fragments arranged by size: 2991 2409 1881 1558 411 398 274 147 105 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2226 2256 5834 5864 6960 Size: 30 165 30 823 30 3578 30 1096 Cuts at: 6960 6990 7295 7325 1178 Size: 30 305 30 4027 Fragments arranged by size: 4027 3578 1096 823 305 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2954 3353 4010 5529 5547 6227 1186 Size: 853 915 399 657 1519 18 680 5133 Fragments arranged by size: 5133 1519 915 853 680 657 399 18 BseYI C'CCAG_C Cuts at: 4185 7358 9377 9873 4185 Size: 3173 2019 496 4486 Fragments arranged by size: 4486 3173 2019 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2739 3377 4268 1163 Size: 573 1003 638 891 7069 Fragments arranged by size: 7069 1003 891 638 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2196 4619 7624 8385 9413 1540 Size: 275 186 195 2423 3005 761 1028 2301 Fragments arranged by size: 3005 2423 2301 1028 761 275 195 186 BsiHKAI G_wGCw'C Cuts at: 2018 2123 2304 2331 2380 2515 3330 3611 4467 Size: 105 181 27 49 135 815 281 856 Cuts at: 4467 4545 5539 5575 6091 6408 8235 8320 9391 Size: 78 994 36 516 317 1827 85 1071 Cuts at: 9391 2018 Size: 2801 Fragments arranged by size: 2801 1827 1071 994 856 815 516 317 281 181 135 105 85 78 49 36 27 BsiWI C'GTAC_G Cuts at: 4403 4403 Size: 10174 BsmI GAATG_Cn' Cuts at: 2771 3679 7685 7778 2771 Size: 908 4006 93 5167 Fragments arranged by size: 5167 4006 908 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2144 4587 2144 Size: 2443 7731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2123 2247 2304 2331 2380 2402 Size: 399 228 105 124 57 27 49 22 Cuts at: 2402 2515 3330 3611 4108 4126 4467 4545 5113 Size: 113 815 281 497 18 341 78 568 Cuts at: 5113 5539 5566 5575 6091 6408 7493 8235 8320 Size: 426 27 9 516 317 1085 742 85 Cuts at: 8320 9391 1391 Size: 1071 2174 Fragments arranged by size: 2174 1085 1071 815 742 568 516 497 426 399 341 317 281 228 124 113 105 85 78 57 49 27 27 22 18 9 BspEI T'CCGG_A Cuts at: 7049 9837 7049 Size: 2788 7386 BspHI T'CATG_A Cuts at: 6561 8063 9793 6561 Size: 1502 1730 6942 Fragments arranged by size: 6942 1730 1502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2862 4432 5007 7529 844 Size: 2018 1570 575 2522 3489 Fragments arranged by size: 3489 2522 2018 1570 575 BsrBI CCG'CTC Cuts at: 1394 1823 2924 6349 8061 1394 Size: 429 1101 3425 1712 3507 Fragments arranged by size: 3507 3425 1712 1101 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2212 4467 5320 5736 6370 8669 8843 Size: 1183 964 2255 853 416 634 2299 174 Cuts at: 8843 65 Size: 1396 Fragments arranged by size: 2299 2255 1396 1183 964 853 634 416 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8818 9005 1700 Size: 110 7008 187 2869 Fragments arranged by size: 7008 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4593 5058 96 Size: 4497 465 5212 Fragments arranged by size: 5212 4497 465 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10174 BssSI C'ACGA_G Cuts at: 3329 8234 9246 3329 Size: 4905 1012 4257 Fragments arranged by size: 4905 4257 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2805 3391 3932 794 Size: 2011 586 541 7036 Fragments arranged by size: 7036 2011 586 541 BstBI TT'CG_AA Cuts at: 5242 8978 5242 Size: 3736 6438 BstEII G'GTnAC_C Cuts at: 1184 5318 1184 Size: 4134 6040 BstXI CCAn_nnnn'nTGG Cuts at: 5860 6784 6875 7268 5860 Size: 924 91 393 8766 Fragments arranged by size: 8766 924 393 91 BstYI r'GATC_y Cuts at: 1972 2584 3463 4455 4497 5418 6322 7504 7871 Size: 612 879 992 42 921 904 1182 367 Cuts at: 7871 8256 8273 9725 9811 9823 10169 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1182 992 921 904 879 612 385 367 346 86 42 17 12 BstZ17I GTA'TAC Cuts at: 4720 6177 4720 Size: 1457 8717 Bsu36I CC'TnA_GG Cuts at: 4935 4935 Size: 10174 BtgI C'CryG_G Cuts at: 513 1065 2004 4266 5178 6517 6580 6936 7063 Size: 552 939 2262 912 1339 63 356 127 Cuts at: 7063 513 Size: 3624 Fragments arranged by size: 3624 2262 1339 939 912 552 356 127 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9435 Fragments arranged by size: 9435 683 56 BtsI GCAGTG_nn' Cuts at: 2302 2578 2727 4069 4080 4257 4329 4341 6700 Size: 276 149 1342 11 177 72 12 2359 Cuts at: 6700 7690 8484 8504 2302 Size: 990 794 20 3972 Fragments arranged by size: 3972 2359 1342 990 794 276 177 149 72 20 12 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10174 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10139 DraI TTT'AAA Cuts at: 7478 7478 Size: 10174 DraIII CAC_nnn'GTG Cuts at: 7393 7411 7393 Size: 18 10156 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9181 809 Size: 507 7865 1802 Fragments arranged by size: 7865 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2421 2623 Size: 54 1425 50 275 18 363 228 202 Cuts at: 2623 4269 4522 4616 5028 6583 6697 7066 7621 Size: 1646 253 94 412 1555 114 369 555 Cuts at: 7621 8510 10139 8 Size: 889 1629 43 Fragments arranged by size: 1646 1629 1555 1425 889 555 412 369 363 275 253 228 202 114 94 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2193 4616 7621 1537 Size: 275 381 2423 3005 4090 Fragments arranged by size: 4090 3005 2423 381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3219 3282 4321 6543 8104 1353 Size: 1866 63 1039 2222 1561 3423 Fragments arranged by size: 3423 2222 1866 1561 1039 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8761 9135 9281 387 Size: 1064 138 7172 374 146 1280 Fragments arranged by size: 7172 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2016 2121 3328 4465 5537 2016 Size: 105 1207 1137 1072 6653 Fragments arranged by size: 6653 1207 1137 1072 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2074 3079 3449 3452 4780 5526 5581 Size: 393 442 1005 370 3 1328 746 55 Cuts at: 5581 5669 5817 6644 6771 6851 7272 7402 8237 Size: 88 148 827 127 80 421 130 835 Cuts at: 8237 8834 9621 9889 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1328 1005 835 827 787 746 597 442 421 393 370 268 148 130 127 88 80 55 3 EcoNI CCTnn'n_nnAGG Cuts at: 2443 6021 2443 Size: 3578 6596 EcoO109I rG'GnC_Cy Cuts at: 2839 3487 3790 5562 5563 6773 6879 6932 2839 Size: 648 303 1772 1 1210 106 53 6081 Fragments arranged by size: 6081 1772 1210 648 303 106 53 1 EcoRI G'AATT_C Cuts at: 7483 7861 7483 Size: 378 9796 EcoRV GAT'ATC Cuts at: 5590 5590 Size: 10174 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3125 5143 5653 5714 6082 6755 3125 Size: 2018 510 61 368 673 6544 Fragments arranged by size: 6544 2018 673 510 368 61 FspI TGC'GCA Cuts at: 5312 8680 5312 Size: 3368 6806 HaeII r_GCGC'y Cuts at: 1678 2029 2061 2870 4195 7446 9321 1678 Size: 351 32 809 1325 3251 1875 2531 Fragments arranged by size: 3251 2531 1875 1325 809 351 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2352 2384 3394 3426 3619 Size: 32 135 32 780 32 1010 32 193 Cuts at: 3619 3651 3967 3999 4511 4543 4624 4656 5834 Size: 32 316 32 512 32 81 32 1178 Cuts at: 5834 5866 5921 5953 5998 6030 7232 7264 8815 Size: 32 55 32 45 32 1202 32 1551 Cuts at: 8815 8847 8889 8921 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1551 1202 1178 1010 780 512 316 193 135 81 55 45 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3305 5464 7518 7764 669 Size: 988 281 1367 2159 2054 246 3079 Fragments arranged by size: 3079 2159 2054 1367 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 10174 HpaI GTT'AAC Cuts at: 7764 7764 Size: 10174 KasI G'GCGC_C Cuts at: 7442 7442 Size: 10174 KpnI G_GTAC'C Cuts at: 7499 7499 Size: 10174 MfeI C'AATT_G Cuts at: 7290 7773 7290 Size: 483 9691 MluI A'CGCG_T Cuts at: 10050 10050 Size: 10174 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2292 4995 6193 6408 7218 7249 7297 9288 Size: 987 2703 1198 215 810 31 48 1991 Cuts at: 9288 9472 1305 Size: 184 2007 Fragments arranged by size: 2703 2007 1991 1198 987 810 215 184 48 31 MscI TGG'CCA Cuts at: 10 64 1489 1832 2423 2625 4271 4524 5030 Size: 54 1425 343 591 202 1646 253 506 Cuts at: 5030 10 Size: 5154 Fragments arranged by size: 5154 1646 1425 591 506 343 253 202 54 MslI CAynn'nnrTG Cuts at: 518 1166 2937 4002 4334 5099 6316 8132 8491 Size: 648 1771 1065 332 765 1217 1816 359 Cuts at: 8491 8650 518 Size: 159 2042 Fragments arranged by size: 2042 1816 1771 1217 1065 765 648 359 332 159 MspA1I CmG'CkG Cuts at: 1606 2126 2311 3154 3388 3436 3544 4532 5252 Size: 520 185 843 234 48 108 988 720 Cuts at: 5252 5352 7440 7590 8267 9415 9660 1606 Size: 100 2088 150 677 1148 245 2120 Fragments arranged by size: 2120 2088 1148 988 843 720 677 520 245 234 185 150 108 100 48 NarI GG'CG_CC Cuts at: 7443 7443 Size: 10174 NcoI C'CATG_G Cuts at: 513 1065 2004 4266 6517 6580 6936 513 Size: 552 939 2262 2251 63 356 3751 Fragments arranged by size: 3751 2262 2251 939 552 356 63 NdeI CA'TA_TG Cuts at: 387 387 Size: 10174 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10174 NotI GC'GGCC_GC Cuts at: 7621 7621 Size: 10174 NsiI A_TGCA'T Cuts at: 3874 5609 3874 Size: 1735 8439 NspI r_CATG'y Cuts at: 1132 1229 3601 5330 5477 6601 7532 9077 1132 Size: 97 2372 1729 147 1124 931 1545 2229 Fragments arranged by size: 2372 2229 1729 1545 1124 931 147 97 PasI CC'CwG_GG Cuts at: 1348 5665 1348 Size: 4317 5857 PciI A'CATG_T Cuts at: 3597 5326 6597 9073 3597 Size: 1729 1271 2476 4698 Fragments arranged by size: 4698 2476 1729 1271 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 10174 PfoI T'CCnGG_A Cuts at: 3426 3468 4389 6799 3426 Size: 42 921 2410 6801 Fragments arranged by size: 6801 2410 921 42 PmeI GTTT'AAAC Cuts at: 7478 7478 Size: 10174 PmlI CAC'GTG Cuts at: 2935 2935 Size: 10174 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4865 4897 6493 6525 8243 8275 9756 9788 4865 Size: 32 1596 32 1718 32 1481 32 5251 Fragments arranged by size: 5251 1718 1596 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2839 3790 6773 2839 Size: 951 2983 6240 Fragments arranged by size: 6240 2983 951 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10174 PsiI TTA'TAA Cuts at: 7744 7744 Size: 10174 PspOMI G'GGCC_C Cuts at: 5562 5562 Size: 10174 PstI C_TGCA'G Cuts at: 830 2573 4336 4639 7526 830 Size: 1743 1763 303 2887 3478 Fragments arranged by size: 3478 2887 1763 1743 303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10174 PvuII CAG'CTG Cuts at: 3154 3436 4532 5252 3154 Size: 282 1096 720 8076 Fragments arranged by size: 8076 1096 720 282 SacI G_AGCT'C Cuts at: 2018 2123 3330 4467 5539 2018 Size: 105 1207 1137 1072 6653 Fragments arranged by size: 6653 1207 1137 1072 105 SalI G'TCGA_C Cuts at: 1936 7516 1936 Size: 5580 4594 SanDI GG'GwC_CC Cuts at: 6773 6773 Size: 10174 SapI GCTCTTCn'nnn_ Cuts at: 3219 3219 Size: 10174 SbfI CC_TGCA'GG Cuts at: 7526 7526 Size: 10174 ScaI AGT'ACT Cuts at: 8422 10134 8422 Size: 1712 8462 SfcI C'TryA_G Cuts at: 826 1045 2443 2569 2793 4332 4635 6838 7522 Size: 219 1398 126 224 1539 303 2203 684 Cuts at: 7522 8657 9338 9529 826 Size: 1135 681 191 1471 Fragments arranged by size: 2203 1539 1471 1398 1135 684 681 303 224 219 191 126 SfoI GGC'GCC Cuts at: 7444 7444 Size: 10174 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10174 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10174 SmaI CCC'GGG Cuts at: 1872 7501 1872 Size: 5629 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4546 8278 9179 9441 820 Size: 197 926 2603 3732 901 262 1553 Fragments arranged by size: 3732 2603 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7965 493 Size: 7472 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10174 SphI G_CATG'C Cuts at: 1132 7532 1132 Size: 6400 3774 SspI AAT'ATT Cuts at: 5 52 3892 7000 8098 5 Size: 47 3840 3108 1098 2081 Fragments arranged by size: 3840 3108 2081 1098 47 StuI AGG'CCT Cuts at: 1517 4574 1517 Size: 3057 7117 StyI C'CwwG_G Cuts at: 513 1065 2004 3243 4266 6517 6580 6936 7613 Size: 552 939 1239 1023 2251 63 356 677 Cuts at: 7613 513 Size: 3074 Fragments arranged by size: 3074 2251 1239 1023 939 677 552 356 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3332 3966 4088 4385 4643 5980 7183 Size: 1255 1752 634 122 297 258 1337 1203 Cuts at: 7183 7422 8201 8218 8371 8556 9001 325 Size: 239 779 17 153 185 445 1498 Fragments arranged by size: 1752 1498 1337 1255 1203 779 634 445 297 258 239 185 153 122 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4593 5058 5922 Size: 274 80 33 51 1443 2616 465 864 Cuts at: 5922 8420 10132 96 Size: 2498 1712 138 Fragments arranged by size: 2616 2498 1712 1443 864 465 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2226 2258 2283 2315 3400 3432 6795 6827 6960 Size: 32 25 32 1085 32 3363 32 133 Cuts at: 6960 6992 7395 7427 10134 10166 2226 Size: 32 403 32 2707 32 2234 Fragments arranged by size: 3363 2707 2234 1085 403 133 32 32 32 32 32 32 32 25 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10174 XbaI T'CTAG_A Cuts at: 4205 6982 7510 4205 Size: 2777 528 6869 Fragments arranged by size: 6869 2777 528 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2275 4264 7076 2275 Size: 1989 2812 5373 Fragments arranged by size: 5373 2812 1989 XhoI C'TCGA_G Cuts at: 1943 4546 1943 Size: 2603 7571 XmaI C'CCGG_G Cuts at: 1870 7499 1870 Size: 5629 4545 XmnI GAAnn'nnTTC Cuts at: 5615 5636 6508 6557 6738 7045 8303 5615 Size: 21 872 49 181 307 1258 7486 Fragments arranged by size: 7486 1258 872 307 181 49 21 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7982 276 Size: 53 83 186 936 6448 2468 Fragments arranged by size: 6448 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BplI FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI