Restriction map for pFN21AB0049
 (Linear) (Six-base) MAPSORT of: KIBB0049.seq  Check: 5261  from: 1  to: 5469



KIBB0049.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AccI GT'mk_AC

Cuts at:      0    2714    4171    5469 
   Size:       2714    1457    1298

  Fragments arranged by size: 

                2714    1457    1298
 
AclI AA'CG_TT

Cuts at:      0     666    2429    5469 
   Size:        666    1763    3040

  Fragments arranged by size: 

                3040    1763     666
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2775    3576    3812    4639    4766    5267    5397    5469 
   Size:       2775     801     236     827     127     501     130      72

  Fragments arranged by size: 

                2775     827     801     501     236     130     127      72
            
 
AfeI AGC'GCT

Cuts at:      0      54     863    5469 
   Size:         54     809    4606

  Fragments arranged by size: 

                4606     809      54
 
AflIII A'CryG_T

Cuts at:      0    1592    3321    3839    4592    5469 
   Size:       1592    1729     518     753     877

  Fragments arranged by size: 

                1729    1592     877     753     518
 
AleI CACnn'nnGTG

Cuts at:      0    2329    3094    5469 
   Size:       2329     765    2375

  Fragments arranged by size: 

                2375    2329     765
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     450    1837    2270    5469 
   Size:        450    1387     433    3199

  Fragments arranged by size: 

                3199    1387     450     433
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1091    1123    3881    3913    4958    4990    5469 
   Size:       1091      32    2758      32    1045      32     479

  Fragments arranged by size: 

                2758    1091    1045     479      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1386    2074    2190    2385    2777    5469 
   Size:       1386     688     116     195     392    2692

  Fragments arranged by size: 

                2692    1386     688     392     195     116
 
ApaI G_GGCC'C

Cuts at:      0    3561    5469 
   Size:       3561    1908
 
ApaLI G'TGCA_C

Cuts at:      0     295    2536    5469 
   Size:        295    2241    2933

  Fragments arranged by size: 

                2933    2241     295
 
ApoI r'AATT_y

Cuts at:      0     573    1924    1949    2893    3202    4251    5469 
   Size:        573    1351      25     944     309    1049    1218

  Fragments arranged by size: 

                1351    1218    1049     944     573     309      25
 
AseI AT'TA_AT

Cuts at:      0    3392    5469 
   Size:       3392    2077
 
AvaI C'yCGr_G

Cuts at:      0    2541    2847    3107    5469 
   Size:       2541     306     260    2362

  Fragments arranged by size: 

                2541    2362     306     260
 
BamHI G'GATC_C

Cuts at:      0    2450    4317    5469 
   Size:       2450    1867    1152

  Fragments arranged by size: 

                2450    1867    1152
 
BanI G'GyrC_C

Cuts at:      0    4468    5437    5469 
   Size:       4468     969      32

  Fragments arranged by size: 

                4468     969      32
 
BanII G_rGCy'C

Cuts at:      0      13     118    1325    2103    2462    3534    3561    5469 
   Size:         13     105    1207     778     359    1072      27    1908

  Fragments arranged by size: 

                1908    1207    1072     778     359     105      27      13
            
 
BbeI G_GCGC'C

Cuts at:      0    5441    5469 
   Size:       5441      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1514    3692    4208    4726    5469 
   Size:       1514    2178     516     518     743

  Fragments arranged by size: 

                2178    1514     743     518     516
 
BbvCI CC'TCA_GC

Cuts at:      0    1744    2378    5469 
   Size:       1744     634    3091

  Fragments arranged by size: 

                3091    1744     634
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     463     497    2223    2257    5469 
   Size:        463      34    1726      34    3212

  Fragments arranged by size: 

                3212    1726     463      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     280     912    4960    5469 
   Size:        280     632    4048     509

  Fragments arranged by size: 

                4048     632     509     280
 
BclI T'GATC_A

Cuts at:      0    2207    3044    5289    5469 
   Size:       2207     837    2245     180

  Fragments arranged by size: 

                2245    2207     837     180
 
BfrBI ATG'CAT

Cuts at:      0    1867    3602    5469 
   Size:       1867    1735    1867

  Fragments arranged by size: 

                1867    1867    1735
 
BglI GCCn_nnn'nGGC

Cuts at:      0      39    2263    5469 
   Size:         39    2224    3206

  Fragments arranged by size: 

                3206    2224      39
 
BglII A'GATC_T

Cuts at:      0    1458    3413    5469 
   Size:       1458    1955    2056

  Fragments arranged by size: 

                2056    1955    1458
 
BlpI GC'TnA_GC

Cuts at:      0      12     117     895    5469 
   Size:         12     105     778    4574

  Fragments arranged by size: 

                4574     778     105      12
 
Bme1580I G_kGCm'C

Cuts at:      0     242     299     397    2121    2540    3108    3561    5469 
   Size:        242      57      98    1724     419     568     453    1908

  Fragments arranged by size: 

                1908    1724     568     453     419     242      98      57
            
 
BmgBI CAC'GTC

Cuts at:      0    2576    3840    5469 
   Size:       2576    1264    1629

  Fragments arranged by size: 

                2576    1629    1264
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    5398    5469 
   Size:       5398      71
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      69    1074    1444    1447    3521    3664    4846    5469 
   Size:         69    1005     370       3    2074     143    1182     623

  Fragments arranged by size: 

                2074    1182    1005     623     370     143      69       3
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     282    1744    2378    5469 
   Size:        282    1462     634    3091

  Fragments arranged by size: 

                3091    1462     634     282
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1390    4717    5469 
   Size:       1390    3327     752

  Fragments arranged by size: 

                3327    1390     752
 
BsaAI yAC'GTr

Cuts at:      0     930    4971    5469 
   Size:        930    4041     498

  Fragments arranged by size: 

                4041     930     498
 
BsaBI GATnn'nnATC

Cuts at:      0    1012    3418    5469 
   Size:       1012    2406    2051

  Fragments arranged by size: 

                2406    2051    1012
 
BsaHI Gr'CG_yC

Cuts at:      0     174     692    5438    5469 
   Size:        174     518    4746      31

  Fragments arranged by size: 

                4746     518     174      31
 
BsaWI w'CCGG_w

Cuts at:      0     649     754    3163    5044    5469 
   Size:        649     105    2409    1881     425

  Fragments arranged by size: 

                2409    1881     649     425     105
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     221     251    3829    3859    4955    4985    5290    5320 
   Size:        221      30    3578      30    1096      30     305      30

Cuts at:   5320    5469 
   Size:        149

  Fragments arranged by size: 

                3578    1096     305     221     149      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     949    1348    2005    3524    3542    4222    5469 
   Size:         34     915     399     657    1519      18     680    1247

  Fragments arranged by size: 

                1519    1247     915     680     657     399      34      18
            
 
BseYI C'CCAG_C

Cuts at:      0    2180    5353    5469 
   Size:       2180    3173     116

  Fragments arranged by size: 

                3173    2180     116
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     734    1372    2263    5469 
   Size:        734     638     891    3206

  Fragments arranged by size: 

                3206     891     734     638
 
BsiEI CG_ry'CG

Cuts at:      0     191    2614    5469 
   Size:        191    2423    2855

  Fragments arranged by size: 

                2855    2423     191
 
BsiHKAI G_wGCw'C

Cuts at:      0      13     118     299     326     375     510    1325    1606 
   Size:         13     105     181      27      49     135     815     281

Cuts at:   1606    2462    2540    3534    3570    4086    4403    5469 
   Size:        856      78     994      36     516     317    1066

  Fragments arranged by size: 

                1066     994     856     815     516     317     281     181
                 135     105      78      49      36      27      13
 
BsiWI C'GTAC_G

Cuts at:      0    2398    5469 
   Size:       2398    3071
 
BsmI GAATG_Cn'

Cuts at:      0     766    1674    5469 
   Size:        766     908    3795

  Fragments arranged by size: 

                3795     908     766
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     139    2582    5469 
   Size:        139    2443    2887

  Fragments arranged by size: 

                2887    2443     139
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     118     242     299     326     375     397     510 
   Size:         13     105     124      57      27      49      22     113

Cuts at:    510    1325    1606    2103    2121    2462    2540    3108    3534 
   Size:        815     281     497      18     341      78     568     426

Cuts at:   3534    3561    3570    4086    4403    5469 
   Size:         27       9     516     317    1066

  Fragments arranged by size: 

                1066     815     568     516     497     426     341     317
                 281     124     113     105      78      57      49      27
                  27      22      18      13       9
 
BspEI T'CCGG_A

Cuts at:      0    5044    5469 
   Size:       5044     425
 
BspHI T'CATG_A

Cuts at:      0    4556    5469 
   Size:       4556     913
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     857    2427    3002    5469 
   Size:        857    1570     575    2467

  Fragments arranged by size: 

                2467    1570     857     575
 
BsrBI CCG'CTC

Cuts at:      0     919    4344    5469 
   Size:        919    3425    1125

  Fragments arranged by size: 

                3425    1125     919
 
BsrDI GCAATG_nn'

Cuts at:      0     207    2462    3315    3731    4365    5469 
   Size:        207    2255     853     416     634    1104

  Fragments arranged by size: 

                2255    1104     853     634     416     207
 
BsrGI T'GTAC_A

Cuts at:      0    2588    3053    5469 
   Size:       2588     465    2416

  Fragments arranged by size: 

                2588    2416     465
 
BssSI C'ACGA_G

Cuts at:      0    1324    5469 
   Size:       1324    4145
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     800    1386    1927    5469 
   Size:        800     586     541    3542

  Fragments arranged by size: 

                3542     800     586     541
 
BstBI TT'CG_AA

Cuts at:      0    3237    5469 
   Size:       3237    2232
 
BstEII G'GTnAC_C

Cuts at:      0    3313    5469 
   Size:       3313    2156
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3855    4779    4870    5263    5469 
   Size:       3855     924      91     393     206

  Fragments arranged by size: 

                3855     924     393     206      91
 
BstYI r'GATC_y

Cuts at:      0     579    1458    2450    2492    3413    4317    5469 
   Size:        579     879     992      42     921     904    1152

  Fragments arranged by size: 

                1152     992     921     904     879     579      42
 
BstZ17I GTA'TAC

Cuts at:      0    2715    4172    5469 
   Size:       2715    1457    1297

  Fragments arranged by size: 

                2715    1457    1297
 
Bsu36I CC'TnA_GG

Cuts at:      0    2930    5469 
   Size:       2930    2539
 
BtgI C'CryG_G

Cuts at:      0    2261    3173    4512    4575    4931    5058    5469 
   Size:       2261     912    1339      63     356     127     411

  Fragments arranged by size: 

                2261    1339     912     411     356     127      63
 
BtsI GCAGTG_nn'

Cuts at:      0     297     573     722    2064    2075    2252    2324    2336 
   Size:        297     276     149    1342      11     177      72      12

Cuts at:   2336    4695    5469 
   Size:       2359     774

  Fragments arranged by size: 

                2359    1342     774     297     276     177     149      72
                  12      11
 
DraIII CAC_nnn'GTG

Cuts at:      0    5388    5406    5469 
   Size:       5388      18      63

  Fragments arranged by size: 

                5388      63      18
 
EaeI y'GGCC_r

Cuts at:      0     188     416     618    2264    2517    2611    3023    4578 
   Size:        188     228     202    1646     253      94     412    1555

Cuts at:   4578    4692    5061    5469 
   Size:        114     369     408

  Fragments arranged by size: 

                1646    1555     412     408     369     253     228     202
                 188     114      94
 
EagI C'GGCC_G

Cuts at:      0     188    2611    5469 
   Size:        188    2423    2858

  Fragments arranged by size: 

                2858    2423     188
 
EarI CTCTTCn'nnn_

Cuts at:      0    1214    1277    2316    4538    5469 
   Size:       1214      63    1039    2222     931

  Fragments arranged by size: 

                2222    1214    1039     931      63
 
EcoICRI GAG'CTC

Cuts at:      0      11     116    1323    2460    3532    5469 
   Size:         11     105    1207    1137    1072    1937

  Fragments arranged by size: 

                1937    1207    1137    1072     105      11
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      69    1074    1444    1447    2775    3521    3576    3664 
   Size:         69    1005     370       3    1328     746      55      88

Cuts at:   3664    3812    4639    4766    4846    5267    5397    5469 
   Size:        148     827     127      80     421     130      72

  Fragments arranged by size: 

                1328    1005     827     746     421     370     148     130
                 127      88      80      72      69      55       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     438    4016    5469 
   Size:        438    3578    1453

  Fragments arranged by size: 

                3578    1453     438
 
EcoO109I rG'GnC_Cy

Cuts at:      0     834    1482    1785    3557    3558    4768    4874    4927 
   Size:        834     648     303    1772       1    1210     106      53

Cuts at:   4927    5469 
   Size:        542

  Fragments arranged by size: 

                1772    1210     834     648     542     303     106      53
                   1
 
EcoRV GAT'ATC

Cuts at:      0    3585    5469 
   Size:       3585    1884
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1120    3138    3648    3709    4077    4750    5469 
   Size:       1120    2018     510      61     368     673     719

  Fragments arranged by size: 

                2018    1120     719     673     510     368      61
 
FspI TGC'GCA

Cuts at:      0    3307    5469 
   Size:       3307    2162
 
HaeII r_GCGC'y

Cuts at:      0      24      56     865    2190    5441    5469 
   Size:         24      32     809    1325    3251      28

  Fragments arranged by size: 

                3251    1325     809      32      28      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     347     379    1389    1421    1614    1646    1962    1994 
   Size:        347      32    1010      32     193      32     316      32

Cuts at:   1994    2506    2538    2619    2651    3829    3861    3916    3948 
   Size:        512      32      81      32    1178      32      55      32

Cuts at:   3948    3993    4025    5227    5259    5469 
   Size:         45      32    1202      32     210

  Fragments arranged by size: 

                1202    1178    1010     512     347     316     210     193
                  81      55      45      32      32      32      32      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1300    3459    5469 
   Size:       1300    2159    2010

  Fragments arranged by size: 

                2159    2010    1300
 
KasI G'GCGC_C

Cuts at:      0    5437    5469 
   Size:       5437      32
 
MfeI C'AATT_G

Cuts at:      0    5285    5469 
   Size:       5285     184
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     287    2990    4188    4403    5213    5244    5292    5469 
   Size:        287    2703    1198     215     810      31      48     177

  Fragments arranged by size: 

                2703    1198     810     287     215     177      48      31
            
 
MscI TGG'CCA

Cuts at:      0     418     620    2266    2519    3025    5469 
   Size:        418     202    1646     253     506    2444

  Fragments arranged by size: 

                2444    1646     506     418     253     202
 
MslI CAynn'nnrTG

Cuts at:      0     932    1997    2329    3094    4311    5469 
   Size:        932    1065     332     765    1217    1158

  Fragments arranged by size: 

                1217    1158    1065     932     765     332
 
MspA1I CmG'CkG

Cuts at:      0     121     306    1149    1383    1431    1539    2527    3247 
   Size:        121     185     843     234      48     108     988     720

Cuts at:   3247    3347    5435    5469 
   Size:        100    2088      34

  Fragments arranged by size: 

                2088     988     843     720     234     185     121     108
                 100      48      34
 
NarI GG'CG_CC

Cuts at:      0    5438    5469 
   Size:       5438      31
 
NcoI C'CATG_G

Cuts at:      0    2261    4512    4575    4931    5469 
   Size:       2261    2251      63     356     538

  Fragments arranged by size: 

                2261    2251     538     356      63
 
NsiI A_TGCA'T

Cuts at:      0    1869    3604    5469 
   Size:       1869    1735    1865

  Fragments arranged by size: 

                1869    1865    1735
 
NspI r_CATG'y

Cuts at:      0    1596    3325    3472    4596    5469 
   Size:       1596    1729     147    1124     873

  Fragments arranged by size: 

                1729    1596    1124     873     147
 
PasI CC'CwG_GG

Cuts at:      0    3660    5469 
   Size:       3660    1809
 
PciI A'CATG_T

Cuts at:      0    1592    3321    4592    5469 
   Size:       1592    1729    1271     877

  Fragments arranged by size: 

                1729    1592    1271     877
 
PfoI T'CCnGG_A

Cuts at:      0    1421    1463    2384    4794    5469 
   Size:       1421      42     921    2410     675

  Fragments arranged by size: 

                2410    1421     921     675      42
 
PmlI CAC'GTG

Cuts at:      0     930    5469 
   Size:        930    4539
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2860    2892    4488    4520    5469 
   Size:       2860      32    1596      32     949

  Fragments arranged by size: 

                2860    1596     949      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     834    1785    4768    5469 
   Size:        834     951    2983     701

  Fragments arranged by size: 

                2983     951     834     701
 
PspOMI G'GGCC_C

Cuts at:      0    3557    5469 
   Size:       3557    1912
 
PstI C_TGCA'G

Cuts at:      0     568    2331    2634    5469 
   Size:        568    1763     303    2835

  Fragments arranged by size: 

                2835    1763     568     303
 
PvuII CAG'CTG

Cuts at:      0    1149    1431    2527    3247    5469 
   Size:       1149     282    1096     720    2222

  Fragments arranged by size: 

                2222    1149    1096     720     282
 
SacI G_AGCT'C

Cuts at:      0      13     118    1325    2462    3534    5469 
   Size:         13     105    1207    1137    1072    1935

  Fragments arranged by size: 

                1935    1207    1137    1072     105      13
 
SanDI GG'GwC_CC

Cuts at:      0    4768    5469 
   Size:       4768     701
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1214    5469 
   Size:       1214    4255
 
SfcI C'TryA_G

Cuts at:      0     438     564     788    2327    2630    4833    5469 
   Size:        438     126     224    1539     303    2203     636

  Fragments arranged by size: 

                2203    1539     636     438     303     224     126
 
SfoI GGC'GCC

Cuts at:      0    5439    5469 
   Size:       5439      30
 
SmlI C'TyrA_G

Cuts at:      0    2541    5469 
   Size:       2541    2928
 
SspI AAT'ATT

Cuts at:      0    1887    4995    5469 
   Size:       1887    3108     474

  Fragments arranged by size: 

                3108    1887     474
 
StuI AGG'CCT

Cuts at:      0    2569    5469 
   Size:       2569    2900
 
StyI C'CwwG_G

Cuts at:      0    1238    2261    4512    4575    4931    5469 
   Size:       1238    1023    2251      63     356     538

  Fragments arranged by size: 

                2251    1238    1023     538     356      63
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1327    1961    2083    2380    2638    3975    5178    5417 
   Size:       1327     634     122     297     258    1337    1203     239

Cuts at:   5417    5469 
   Size:         52

  Fragments arranged by size: 

                1337    1327    1203     634     297     258     239     122
                  52
 
TatI w'GTAC_w

Cuts at:      0    2588    3053    3917    5469 
   Size:       2588     465     864    1552

  Fragments arranged by size: 

                2588    1552     864     465
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     221     253     278     310    1395    1427    4790    4822 
   Size:        221      32      25      32    1085      32    3363      32

Cuts at:   4822    4955    4987    5390    5422    5469 
   Size:        133      32     403      32      47

  Fragments arranged by size: 

                3363    1085     403     221     133      47      32      32
                  32      32      32      32      25
 
XbaI T'CTAG_A

Cuts at:      0    2200    4977    5469 
   Size:       2200    2777     492

  Fragments arranged by size: 

                2777    2200     492
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     270    2259    5071    5469 
   Size:        270    1989    2812     398

  Fragments arranged by size: 

                2812    1989     398     270
 
XhoI C'TCGA_G

Cuts at:      0    2541    5469 
   Size:       2541    2928
 
XmnI GAAnn'nnTTC

Cuts at:      0    3610    3631    4503    4552    4733    5040    5469 
   Size:       3610      21     872      49     181     307     429

  Fragments arranged by size: 

                3610     872     429     307     181      49      21
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI   DraIII
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII     KasI     MfeI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NsiI     NspI     PasI
     PciI     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SapI     SfcI     SfoI     SmlI     SspI     StuI
     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I    AflII     AgeI     AhdI     AscI    AsiSI
    AvrII     BaeI     BmtI     BplI    BpuEI    BsrFI   BssHII    BtgZI
     ClaI    CspCI     DraI     DrdI     EciI    EcoRI     FseI    FspAI
  HindIII     HpaI     KpnI     MluI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI    PflMI     PmeI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI  Tth111I     XmaI     ZraI
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