(Circular) (Six-base) MAPSORT of: pFN21AB0019.seq Check: 1365 from: 1 to: 13234 pFN21AB0019 13234 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 9958 9958 Size: 13234 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 11044 278 Size: 53 83 186 936 9508 2468 Fragments arranged by size: 9508 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2695 3988 4727 8959 9115 10577 1937 Size: 758 1293 739 4232 156 1462 4594 Fragments arranged by size: 4594 4232 1462 1293 758 739 156 Acc65I G'GTAC_C Cuts at: 2618 10555 2618 Size: 7937 5297 AclI AA'CG_TT Cuts at: 1563 5440 11361 11734 1563 Size: 3877 5921 373 3063 Fragments arranged by size: 5921 3877 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2474 4986 5469 5874 5973 6018 6054 Size: 440 402 2512 483 405 99 45 36 Cuts at: 6054 7769 8127 10383 11297 12681 12949 1632 Size: 1715 358 2256 914 1384 268 1917 Fragments arranged by size: 2512 2256 1917 1715 1384 914 483 440 405 402 358 268 99 45 36 AflII C'TTAA_G Cuts at: 820 1017 8762 9358 820 Size: 197 7745 596 4696 Fragments arranged by size: 7745 4696 596 197 AflIII A'CryG_T Cuts at: 1203 4841 7364 7366 9775 12133 13110 1203 Size: 3638 2523 2 2409 2358 977 1327 Fragments arranged by size: 3638 2523 2409 2358 1327 977 2 AgeI A'CCGG_T Cuts at: 12065 12065 Size: 13234 AhdI GACnn_n'nnGTC Cuts at: 11963 11963 Size: 13234 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 13234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3411 3651 7281 1184 Size: 2227 240 3630 7137 Fragments arranged by size: 7137 3630 2227 240 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 10477 10509 10477 Size: 32 13202 AlwNI CAG_nnn'CTG Cuts at: 3326 5897 6458 7844 9086 10977 12549 3326 Size: 2571 561 1386 1242 1891 1572 4011 Fragments arranged by size: 4011 2571 1891 1572 1386 1242 561 ApaLI G'TGCA_C Cuts at: 3837 11291 12447 3837 Size: 7454 1156 4624 Fragments arranged by size: 7454 4624 1156 ApoI r'AATT_y Cuts at: 1493 2533 2664 3118 3652 3784 4828 6062 7052 Size: 1040 131 454 534 132 1044 1234 990 Cuts at: 7052 7447 7861 8296 8329 8502 8628 9860 10543 Size: 395 414 435 33 173 126 1232 683 Cuts at: 10543 10769 10921 13030 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1234 1232 1044 1040 990 683 534 454 435 414 395 226 173 152 132 131 126 33 AseI AT'TA_AT Cuts at: 160 1032 5367 7188 11788 160 Size: 872 4335 1821 4600 1606 Fragments arranged by size: 4600 4335 1821 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 13234 AvaI C'yCGr_G Cuts at: 1870 1943 3123 3141 8679 9681 10559 1870 Size: 73 1180 18 5538 1002 878 4545 Fragments arranged by size: 5538 4545 1180 1002 878 73 18 AvrII C'CTAG_G Cuts at: 10373 10373 Size: 13234 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 8043 8076 8043 Size: 33 13201 BamHI G'GATC_C Cuts at: 10564 10931 10564 Size: 367 12867 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2618 9946 10555 12010 618 Size: 325 217 627 831 7328 609 1455 1842 Fragments arranged by size: 7328 1842 1455 831 627 609 325 217 BanII G_rGCy'C Cuts at: 1391 7980 10553 1391 Size: 6589 2573 4072 Fragments arranged by size: 6589 4072 2573 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2510 3053 3624 4716 5631 7263 7462 Size: 971 611 543 571 1092 915 1632 199 Cuts at: 7462 9255 9571 928 Size: 1793 316 4591 Fragments arranged by size: 4591 1793 1632 1092 971 915 611 571 543 316 199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 11425 11459 1903 Size: 34 9488 34 3678 Fragments arranged by size: 9488 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 9832 11126 12336 9832 Size: 1294 1210 10730 Fragments arranged by size: 10730 1294 1210 BclI T'GATC_A Cuts at: 1548 2023 8461 1548 Size: 475 6438 6321 Fragments arranged by size: 6438 6321 475 BfrBI ATG'CAT Cuts at: 4043 7185 4043 Size: 3142 10092 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3551 11845 136 Size: 107 122 71 631 605 1879 8294 1525 Fragments arranged by size: 8294 1879 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3339 4641 4877 8227 10013 10022 13229 3339 Size: 1302 236 3350 1786 9 3207 3344 Fragments arranged by size: 3350 3344 3207 1786 1302 236 9 BlpI GC'TnA_GC Cuts at: 2775 4302 10651 2775 Size: 1527 6349 5358 Fragments arranged by size: 6349 5358 1527 Bme1580I G_kGCm'C Cuts at: 1790 3841 7042 11295 12451 1790 Size: 2051 3201 4253 1156 2573 Fragments arranged by size: 4253 3201 2573 2051 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 13234 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6258 7801 8051 Size: 416 53 83 399 24 4841 1543 250 Cuts at: 8051 10965 11923 442 Size: 2914 958 1753 Fragments arranged by size: 4841 2914 1753 1543 958 416 399 250 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3011 1056 Size: 1955 11279 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2757 9211 2757 Size: 6454 6780 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2274 2346 6477 6567 7038 8754 9093 9432 Size: 1035 72 4131 90 471 1716 339 339 Cuts at: 9432 10410 11894 1239 Size: 978 1484 2579 Fragments arranged by size: 4131 2579 1716 1484 1035 978 471 339 339 90 72 Bpu10I CC'TnA_GC Cuts at: 6405 10497 6405 Size: 4092 9142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2435 4109 4228 5249 7089 7995 8053 8156 8710 Size: 1674 119 1021 1840 906 58 103 554 Cuts at: 8710 10288 11359 12224 12522 2435 Size: 1578 1071 865 298 3147 Fragments arranged by size: 3147 1840 1674 1578 1071 1021 906 865 554 298 119 103 58 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5470 6134 8251 9600 11897 882 Size: 617 3971 664 2117 1349 2297 2219 Fragments arranged by size: 3971 2297 2219 2117 1349 664 617 BsaAI yAC'GTr Cuts at: 493 1204 6770 7367 11025 493 Size: 711 5566 597 3658 2702 Fragments arranged by size: 5566 3658 2702 711 597 BsaBI GATnn'nnATC Cuts at: 2311 8911 13228 2311 Size: 6600 4317 2317 Fragments arranged by size: 6600 4317 2317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 11041 11423 12058 275 Size: 53 83 186 936 9508 382 635 1451 Fragments arranged by size: 9508 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3870 7882 11667 12065 12339 12486 12897 3870 Size: 4012 3785 398 274 147 411 4207 Fragments arranged by size: 4207 4012 3785 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 7011 7041 8797 8827 1178 Size: 30 165 30 5608 30 1756 30 5585 Fragments arranged by size: 5608 5585 1756 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3059 4495 6321 7047 8713 8835 1186 Size: 1873 1436 1826 726 1666 122 5585 Fragments arranged by size: 5585 1873 1826 1666 1436 726 122 BseYI C'CCAG_C Cuts at: 4499 5005 8943 12437 12933 4499 Size: 506 3938 3494 496 4800 Fragments arranged by size: 4800 3938 3494 506 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2353 2886 3177 5694 7263 1163 Size: 573 617 533 291 2517 1569 7134 Fragments arranged by size: 7134 2517 1569 617 573 533 291 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 10684 11445 12473 1540 Size: 275 186 8683 761 1028 2301 Fragments arranged by size: 8683 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2647 3589 3841 5249 6103 6529 9657 11295 11380 Size: 942 252 1408 854 426 3128 1638 85 Cuts at: 11380 12451 2647 Size: 1071 3430 Fragments arranged by size: 3430 3128 1638 1408 1071 942 854 426 252 85 BsmI GAATG_Cn' Cuts at: 7187 9169 10745 10838 7187 Size: 1982 1576 93 9583 Fragments arranged by size: 9583 1982 1576 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2647 3589 3841 5249 6103 6529 7042 Size: 399 857 942 252 1408 854 426 513 Cuts at: 7042 7980 9657 10553 11295 11380 12451 1391 Size: 938 1677 896 742 85 1071 2174 Fragments arranged by size: 2174 1677 1408 1071 942 938 896 857 854 742 513 426 399 252 85 BspEI T'CCGG_A Cuts at: 12897 12897 Size: 13234 BspHI T'CATG_A Cuts at: 2977 3283 11123 12853 2977 Size: 306 7840 1730 3358 Fragments arranged by size: 7840 3358 1730 306 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2630 3619 8947 9958 10589 844 Size: 1786 989 5328 1011 631 3489 Fragments arranged by size: 5328 3489 1786 1011 989 631 BsrBI CCG'CTC Cuts at: 1394 1823 2779 11121 1394 Size: 429 956 8342 3507 Fragments arranged by size: 8342 3507 956 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2429 3097 3405 3426 3796 8148 11729 Size: 1183 1181 668 308 21 370 4352 3581 Cuts at: 11729 11903 65 Size: 174 1396 Fragments arranged by size: 4352 3581 1396 1183 1181 668 370 308 174 21 BsrFI r'CCGG_y Cuts at: 1700 1810 2759 11878 12065 1700 Size: 110 949 9119 187 2869 Fragments arranged by size: 9119 2869 949 187 110 BsrGI T'GTAC_A Cuts at: 96 6846 96 Size: 6750 6484 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 13234 BssSI C'ACGA_G Cuts at: 11294 12306 11294 Size: 1012 12222 BstAPI GCAn_nnn'nTGC Cuts at: 794 5897 6380 6422 794 Size: 5103 483 42 7606 Fragments arranged by size: 7606 5103 483 42 BstBI TT'CG_AA Cuts at: 8713 12038 8713 Size: 3325 9909 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 13234 BstXI CCAn_nnnn'nTGG Cuts at: 4675 8206 8882 4675 Size: 3531 676 9027 Fragments arranged by size: 9027 3531 676 BstYI r'GATC_y Cuts at: 1972 2306 3339 4641 4877 5956 8227 8912 10013 Size: 334 1033 1302 236 1079 2271 685 1101 Cuts at: 10013 10022 10416 10564 10931 11316 11333 12785 12871 Size: 9 394 148 367 385 17 1452 86 Cuts at: 12871 12883 13229 1972 Size: 12 346 1977 Fragments arranged by size: 2271 1977 1452 1302 1101 1079 1033 685 394 385 367 346 334 236 148 86 17 12 9 BstZ17I GTA'TAC Cuts at: 2696 2696 Size: 13234 Bsu36I CC'TnA_GG Cuts at: 9079 9898 9079 Size: 819 12415 BtgI C'CryG_G Cuts at: 513 1065 2004 6480 6920 10149 513 Size: 552 939 4476 440 3229 3598 Fragments arranged by size: 4476 3598 3229 939 552 440 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2615 487 Size: 683 56 1389 11106 Fragments arranged by size: 11106 1389 683 56 BtsI GCAGTG_nn' Cuts at: 4650 5653 5985 6241 10138 10750 11544 11564 4650 Size: 1003 332 256 3897 612 794 20 6320 Fragments arranged by size: 6320 3897 1003 794 612 332 256 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 13234 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5328 5363 529 Size: 35 4764 35 8400 Fragments arranged by size: 8400 4764 35 35 DraI TTT'AAA Cuts at: 4896 5547 7401 10538 4896 Size: 651 1854 3137 7592 Fragments arranged by size: 7592 3137 1854 651 DraIII CAC_nnn'GTG Cuts at: 10325 10325 Size: 13234 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 12241 809 Size: 507 10925 1802 Fragments arranged by size: 10925 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 8977 10406 10681 Size: 54 1425 50 275 18 7147 1429 275 Cuts at: 10681 11570 13199 8 Size: 889 1629 43 Fragments arranged by size: 7147 1629 1429 1425 889 275 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 10681 1537 Size: 275 8869 4090 Fragments arranged by size: 8869 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2359 3378 4263 4513 5466 5502 11164 1353 Size: 1006 1019 885 250 953 36 5662 3423 Fragments arranged by size: 5662 3423 1019 1006 953 885 250 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 11821 12195 12341 387 Size: 1064 138 10232 374 146 1280 Fragments arranged by size: 10232 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2274 2346 2474 4986 5469 5874 Size: 393 440 202 72 128 2512 483 405 Cuts at: 5874 5973 6018 6054 6477 6567 7038 7769 8127 Size: 99 45 36 423 90 471 731 358 Cuts at: 8127 8754 9093 9432 10383 10410 11297 11894 12681 Size: 627 339 339 951 27 887 597 787 Cuts at: 12681 12949 1239 Size: 268 1524 Fragments arranged by size: 2512 1524 951 887 787 731 627 597 483 471 440 423 405 393 358 339 339 268 202 128 99 90 72 45 36 27 EcoNI CCTnn'n_nnAGG Cuts at: 7080 7551 8120 8790 7080 Size: 471 569 670 11524 Fragments arranged by size: 11524 670 569 471 EcoO109I rG'GnC_Cy Cuts at: 6688 9432 6688 Size: 2744 10490 EcoRI G'AATT_C Cuts at: 2664 8502 10543 10921 2664 Size: 5838 2041 378 4977 Fragments arranged by size: 5838 4977 2041 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5241 5403 7536 9732 5241 Size: 162 2133 2196 8743 Fragments arranged by size: 8743 2196 2133 162 FspI TGC'GCA Cuts at: 11740 11740 Size: 13234 HaeII r_GCGC'y Cuts at: 1678 9580 10504 12381 1678 Size: 7902 924 1877 2531 Fragments arranged by size: 7902 2531 1877 924 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2723 2755 2908 2940 2978 Size: 32 135 32 1151 32 153 32 38 Cuts at: 2978 3010 3086 3118 4418 4450 5642 5674 5706 Size: 32 76 32 1300 32 1192 32 32 Cuts at: 5706 9609 9641 10408 10440 11875 11907 11949 11981 Size: 3903 32 767 32 1435 32 42 32 Cuts at: 11981 1373 Size: 2626 Fragments arranged by size: 3903 2626 1435 1300 1192 1151 767 153 135 76 42 38 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 7621 9327 10578 10824 669 Size: 988 281 5683 1706 1251 246 3079 Fragments arranged by size: 5683 3079 1706 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 4246 4522 8385 748 Size: 3498 276 3863 5597 Fragments arranged by size: 5597 3863 3498 276 HpaI GTT'AAC Cuts at: 10824 10824 Size: 13234 KpnI G_GTAC'C Cuts at: 2622 10559 2622 Size: 7937 5297 MfeI C'AATT_G Cuts at: 2567 10833 2567 Size: 8266 4968 MluI A'CGCG_T Cuts at: 13110 13110 Size: 13234 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3682 4803 5296 7734 12348 12532 1305 Size: 2377 1121 493 2438 4614 184 2007 Fragments arranged by size: 4614 2438 2377 2007 1121 493 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 11412 Fragments arranged by size: 11412 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3090 3393 5046 11192 11551 11710 518 Size: 648 1924 303 1653 6146 359 159 2042 Fragments arranged by size: 6146 2042 1924 1653 648 359 303 159 MspA1I CmG'CkG Cuts at: 1606 3033 5852 5951 6838 10650 11327 12475 12720 Size: 1427 2819 99 887 3812 677 1148 245 Cuts at: 12720 1606 Size: 2120 Fragments arranged by size: 3812 2819 2120 1427 1148 887 677 245 99 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 11743 Fragments arranged by size: 11743 939 552 NdeI CA'TA_TG Cuts at: 387 2020 5043 5634 387 Size: 1633 3023 591 7987 Fragments arranged by size: 7987 3023 1633 591 NheI G'CTAG_C Cuts at: 1052 3007 1052 Size: 1955 11279 NotI GC'GGCC_GC Cuts at: 10681 10681 Size: 13234 NruI TCG'CGA Cuts at: 2998 2998 Size: 13234 NsiI A_TGCA'T Cuts at: 4045 7187 4045 Size: 3142 10092 NspI r_CATG'y Cuts at: 1132 1229 3398 4047 4123 4845 6893 8373 8668 Size: 97 2169 649 76 722 2048 1480 295 Cuts at: 8668 9779 10592 12137 1132 Size: 1111 813 1545 2229 Fragments arranged by size: 2229 2169 2048 1545 1480 1111 813 722 649 295 97 76 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 13234 PciI A'CATG_T Cuts at: 4841 9775 12133 4841 Size: 4934 2358 5942 Fragments arranged by size: 5942 4934 2358 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 5282 6236 1108 Size: 1524 2650 954 8106 Fragments arranged by size: 8106 2650 1524 954 PfoI T'CCnGG_A Cuts at: 4612 8704 4612 Size: 4092 9142 PmeI GTTT'AAAC Cuts at: 10538 10538 Size: 13234 PmlI CAC'GTG Cuts at: 7367 7367 Size: 13234 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 11303 11335 12816 12848 11303 Size: 32 1481 32 11689 Fragments arranged by size: 11689 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 6688 9432 6688 Size: 2744 10490 PshAI GACnn'nnGTC Cuts at: 1613 4031 1613 Size: 2418 10816 PsiI TTA'TAA Cuts at: 6257 8589 10804 6257 Size: 2332 2215 8687 Fragments arranged by size: 8687 2332 2215 PspXI vC'TCGA_Gb Cuts at: 9681 9681 Size: 13234 PstI C_TGCA'G Cuts at: 830 2014 4177 4657 6018 6418 6430 10586 830 Size: 1184 2163 480 1361 400 12 4156 3478 Fragments arranged by size: 4156 3478 2163 1361 1184 480 400 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 13234 PvuII CAG'CTG Cuts at: 3033 5852 5951 6838 3033 Size: 2819 99 887 9429 Fragments arranged by size: 9429 2819 887 99 SalI G'TCGA_C Cuts at: 1936 10576 1936 Size: 8640 4594 SapI GCTCTTCn'nnn_ Cuts at: 3378 3378 Size: 13234 SbfI CC_TGCA'GG Cuts at: 10586 10586 Size: 13234 ScaI AGT'ACT Cuts at: 2145 2395 6490 9061 9880 11482 13194 2145 Size: 250 4095 2571 819 1602 1712 2185 Fragments arranged by size: 4095 2571 2185 1712 1602 819 250 SexAI A'CCwGG_T Cuts at: 7248 7248 Size: 13234 SfcI C'TryA_G Cuts at: 826 1045 2010 3419 3481 3828 4173 4488 4653 Size: 219 965 1409 62 347 345 315 165 Cuts at: 4653 6014 6414 6426 7077 9546 10269 10582 11717 Size: 1361 400 12 651 2469 723 313 1135 Cuts at: 11717 12398 12589 826 Size: 681 191 1471 Fragments arranged by size: 2469 1471 1409 1361 1135 965 723 681 651 400 347 345 315 313 219 191 165 62 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 13234 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 13234 SmaI CCC'GGG Cuts at: 1872 3125 10561 1872 Size: 1253 7436 4545 Fragments arranged by size: 7436 4545 1253 SmlI C'TyrA_G Cuts at: 820 1017 1943 2414 4124 4243 5264 7104 8010 Size: 197 926 471 1710 119 1021 1840 906 Cuts at: 8010 8068 8171 8679 8725 8762 9358 9681 10303 Size: 58 103 508 46 37 596 323 622 Cuts at: 10303 11338 12239 12501 820 Size: 1035 901 262 1553 Fragments arranged by size: 1840 1710 1553 1035 1021 926 906 901 622 596 508 471 323 262 197 119 103 58 46 37 SnaBI TAC'GTA Cuts at: 493 11025 493 Size: 10532 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 13234 SphI G_CATG'C Cuts at: 1132 4123 10592 1132 Size: 2991 6469 3774 Fragments arranged by size: 6469 3774 2991 SspI AAT'ATT Cuts at: 5 52 4228 5096 11158 5 Size: 47 4176 868 6062 2081 Fragments arranged by size: 6062 4176 2081 868 47 StuI AGG'CCT Cuts at: 1517 4204 1517 Size: 2687 10547 StyI C'CwwG_G Cuts at: 513 1065 2004 2934 4347 7464 9041 10373 10673 Size: 552 939 930 1413 3117 1577 1332 300 Cuts at: 10673 513 Size: 3074 Fragments arranged by size: 3117 3074 1577 1413 1332 939 930 552 300 SwaI ATTT'AAAT Cuts at: 7401 7401 Size: 13234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6124 7297 8198 9967 11261 11278 11431 Size: 1255 4544 1173 901 1769 1294 17 153 Cuts at: 11431 11616 12061 325 Size: 185 445 1498 Fragments arranged by size: 4544 1769 1498 1294 1255 1173 901 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2143 2393 4212 Size: 274 80 33 51 1443 166 250 1819 Cuts at: 4212 5219 6164 6488 6846 7091 8525 8729 9059 Size: 1007 945 324 358 245 1434 204 330 Cuts at: 9059 9878 11480 13192 96 Size: 819 1602 1712 138 Fragments arranged by size: 1819 1712 1602 1443 1434 1007 945 819 358 330 324 274 250 245 204 166 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2922 2954 5548 5580 8797 8829 13194 13226 2922 Size: 32 2594 32 3217 32 4365 32 2930 Fragments arranged by size: 4365 3217 2930 2594 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 7250 1316 Size: 5934 7300 XbaI T'CTAG_A Cuts at: 7597 8378 8903 8915 9254 9410 9614 10570 7597 Size: 781 525 12 339 156 204 956 10261 Fragments arranged by size: 10261 956 781 525 339 204 156 12 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2566 2941 3083 5279 7138 10147 2566 Size: 375 142 2196 1859 3009 5653 Fragments arranged by size: 5653 3009 2196 1859 375 142 XhoI C'TCGA_G Cuts at: 1943 8679 9681 1943 Size: 6736 1002 5496 Fragments arranged by size: 6736 5496 1002 XmaI C'CCGG_G Cuts at: 1870 3123 10559 1870 Size: 1253 7436 4545 Fragments arranged by size: 7436 4545 1253 XmnI GAAnn'nnTTC Cuts at: 4621 7582 8077 9262 11363 4621 Size: 2961 495 1185 2101 6492 Fragments arranged by size: 6492 2961 2101 1185 495 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 11042 276 Size: 53 83 186 936 9508 2468 Fragments arranged by size: 9508 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BbvCI BsiWI BsmBI EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PspOMI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI