Restriction map for pFN21AB0019
 (Linear) (Six-base) MAPSORT of: KIBB0019.seq  Check: 578  from: 1  to: 8529



KIBB0019.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    7953    8529 
   Size:       7953     576
 
AccI GT'mk_AC

Cuts at:      0     690    1983    2722    6954    7110    8529 
   Size:        690    1293     739    4232     156    1419

  Fragments arranged by size: 

                4232    1419    1293     739     690     156
 
Acc65I G'GTAC_C

Cuts at:      0     613    8529 
   Size:        613    7916
 
AclI AA'CG_TT

Cuts at:      0    3435    8529 
   Size:       3435    5094
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     469    2981    3464    3869    3968    4013    4049 
   Size:         67     402    2512     483     405      99      45      36

Cuts at:   4049    5764    6122    8378    8529 
   Size:       1715     358    2256     151

  Fragments arranged by size: 

                2512    2256    1715     483     405     402     358     151
                  99      67      45      36
 
AflII C'TTAA_G

Cuts at:      0    6757    7353    8529 
   Size:       6757     596    1176

  Fragments arranged by size: 

                6757    1176     596
 
AflIII A'CryG_T

Cuts at:      0    2836    5359    5361    7770    8529 
   Size:       2836    2523       2    2409     759

  Fragments arranged by size: 

                2836    2523    2409     759       2
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1406    1646    5276    8529 
   Size:       1406     240    3630    3253

  Fragments arranged by size: 

                3630    3253    1406     240
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    8472    8504    8529 
   Size:       8472      32      25

  Fragments arranged by size: 

                8472      32      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1321    3892    4453    5839    7081    8529 
   Size:       1321    2571     561    1386    1242    1448

  Fragments arranged by size: 

                2571    1448    1386    1321    1242     561
 
ApaLI G'TGCA_C

Cuts at:      0    1832    8529 
   Size:       1832    6697
 
ApoI r'AATT_y

Cuts at:      0     528     659    1113    1647    1779    2823    4057    5047 
   Size:        528     131     454     534     132    1044    1234     990

Cuts at:   5047    5442    5856    6291    6324    6497    6623    7855    8529 
   Size:        395     414     435      33     173     126    1232     674

  Fragments arranged by size: 

                1234    1232    1044     990     674     534     528     454
                 435     414     395     173     132     131     126      33
            
 
AseI AT'TA_AT

Cuts at:      0    3362    5183    8529 
   Size:       3362    1821    3346

  Fragments arranged by size: 

                3362    3346    1821
 
AvaI C'yCGr_G

Cuts at:      0    1118    1136    6674    7676    8529 
   Size:       1118      18    5538    1002     853

  Fragments arranged by size: 

                5538    1118    1002     853      18
 
AvrII C'CTAG_G

Cuts at:      0    8368    8529 
   Size:       8368     161
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    6038    6071    8529 
   Size:       6038      33    2458

  Fragments arranged by size: 

                6038    2458      33
 
BanI G'GyrC_C

Cuts at:      0     613    7941    8529 
   Size:        613    7328     588

  Fragments arranged by size: 

                7328     613     588
 
BanII G_rGCy'C

Cuts at:      0    5975    8529 
   Size:       5975    2554
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     505    1048    1619    2711    3626    5258    5457    7250 
   Size:        505     543     571    1092     915    1632     199    1793

Cuts at:   7250    7566    8529 
   Size:        316     963

  Fragments arranged by size: 

                1793    1632    1092     963     915     571     543     505
                 316     199
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    7827    8529 
   Size:       7827     702
 
BclI T'GATC_A

Cuts at:      0      18    6456    8529 
   Size:         18    6438    2073

  Fragments arranged by size: 

                6438    2073      18
 
BfrBI ATG'CAT

Cuts at:      0    2038    5180    8529 
   Size:       2038    3142    3349

  Fragments arranged by size: 

                3349    3142    2038
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1546    8529 
   Size:       1546    6983
 
BglII A'GATC_T

Cuts at:      0    1334    2636    2872    6222    8008    8017    8529 
   Size:       1334    1302     236    3350    1786       9     512

  Fragments arranged by size: 

                3350    1786    1334    1302     512     236       9
 
BlpI GC'TnA_GC

Cuts at:      0     770    2297    8529 
   Size:        770    1527    6232

  Fragments arranged by size: 

                6232    1527     770
 
Bme1580I G_kGCm'C

Cuts at:      0    1836    5037    8529 
   Size:       1836    3201    3492

  Fragments arranged by size: 

                3492    3201    1836
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    4253    5796    6046    8529 
   Size:       4253    1543     250    2483

  Fragments arranged by size: 

                4253    2483    1543     250
 
BmtI G_CTAG'C

Cuts at:      0    1006    8529 
   Size:       1006    7523
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     752    7206    8529 
   Size:        752    6454    1323

  Fragments arranged by size: 

                6454    1323     752
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     341    4472    4562    5033    6749    7088    7427 
   Size:        269      72    4131      90     471    1716     339     339

Cuts at:   7427    8405    8529 
   Size:        978     124

  Fragments arranged by size: 

                4131    1716     978     471     339     339     269     124
                  90      72
 
Bpu10I CC'TnA_GC

Cuts at:      0    4400    8492    8529 
   Size:       4400    4092      37

  Fragments arranged by size: 

                4400    4092      37
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430    2104    2223    3244    5084    5990    6048    6151 
   Size:        430    1674     119    1021    1840     906      58     103

Cuts at:   6151    6705    8283    8529 
   Size:        554    1578     246

  Fragments arranged by size: 

                1840    1674    1578    1021     906     554     430     246
                 119     103      58
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3465    4129    6246    7595    8529 
   Size:       3465     664    2117    1349     934

  Fragments arranged by size: 

                3465    2117    1349     934     664
 
BsaAI yAC'GTr

Cuts at:      0    4765    5362    8529 
   Size:       4765     597    3167

  Fragments arranged by size: 

                4765    3167     597
 
BsaBI GATnn'nnATC

Cuts at:      0     306    6906    8529 
   Size:        306    6600    1623

  Fragments arranged by size: 

                6600    1623     306
 
BsaWI w'CCGG_w

Cuts at:      0    1865    5877    8529 
   Size:       1865    4012    2652

  Fragments arranged by size: 

                4012    2652    1865
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    5006    5036    6792    6822    8529 
   Size:       5006      30    1756      30    1707

  Fragments arranged by size: 

                5006    1756    1707      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1054    2490    4316    5042    6708    6830    8529 
   Size:       1054    1436    1826     726    1666     122    1699

  Fragments arranged by size: 

                1826    1699    1666    1436    1054     726     122
 
BseYI C'CCAG_C

Cuts at:      0    2494    3000    6938    8529 
   Size:       2494     506    3938    1591

  Fragments arranged by size: 

                3938    2494    1591     506
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     348     881    1172    3689    5258    8529 
   Size:        348     533     291    2517    1569    3271

  Fragments arranged by size: 

                3271    2517    1569     533     348     291
 
BsiHKAI G_wGCw'C

Cuts at:      0     642    1584    1836    3244    4098    4524    7652    8529 
   Size:        642     942     252    1408     854     426    3128     877

  Fragments arranged by size: 

                3128    1408     942     877     854     642     426     252
            
 
BsmI GAATG_Cn'

Cuts at:      0    5182    7164    8529 
   Size:       5182    1982    1365

  Fragments arranged by size: 

                5182    1982    1365
 
Bsp1286I G_dGCh'C

Cuts at:      0     642    1584    1836    3244    4098    4524    5037    5975 
   Size:        642     942     252    1408     854     426     513     938

Cuts at:   5975    7652    8529 
   Size:       1677     877

  Fragments arranged by size: 

                1677    1408     942     938     877     854     642     513
                 426     252
 
BspHI T'CATG_A

Cuts at:      0     972    1278    8529 
   Size:        972     306    7251

  Fragments arranged by size: 

                7251     972     306
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     625    1614    6942    7953    8529 
   Size:        625     989    5328    1011     576

  Fragments arranged by size: 

                5328    1011     989     625     576
 
BsrBI CCG'CTC

Cuts at:      0     774    8529 
   Size:        774    7755
 
BsrDI GCAATG_nn'

Cuts at:      0     424    1092    1400    1421    1791    6143    8529 
   Size:        424     668     308      21     370    4352    2386

  Fragments arranged by size: 

                4352    2386     668     424     370     308      21
 
BsrFI r'CCGG_y

Cuts at:      0     754    8529 
   Size:        754    7775
 
BsrGI T'GTAC_A

Cuts at:      0    4841    8529 
   Size:       4841    3688
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3892    4375    4417    8529 
   Size:       3892     483      42    4112

  Fragments arranged by size: 

                4112    3892     483      42
 
BstBI TT'CG_AA

Cuts at:      0    6708    8529 
   Size:       6708    1821
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2670    6201    6877    8529 
   Size:       2670    3531     676    1652

  Fragments arranged by size: 

                3531    2670    1652     676
 
BstYI r'GATC_y

Cuts at:      0     301    1334    2636    2872    3951    6222    6907    8008 
   Size:        301    1033    1302     236    1079    2271     685    1101

Cuts at:   8008    8017    8411    8529 
   Size:          9     394     118

  Fragments arranged by size: 

                2271    1302    1101    1079    1033     685     394     301
                 236     118       9
 
BstZ17I GTA'TAC

Cuts at:      0     691    8529 
   Size:        691    7838
 
Bsu36I CC'TnA_GG

Cuts at:      0    7074    7893    8529 
   Size:       7074     819     636

  Fragments arranged by size: 

                7074     819     636
 
BtgI C'CryG_G

Cuts at:      0    4475    4915    8144    8529 
   Size:       4475     440    3229     385

  Fragments arranged by size: 

                4475    3229     440     385
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     610    8529 
   Size:        610    7919
 
BtsI GCAGTG_nn'

Cuts at:      0    2645    3648    3980    4236    8133    8529 
   Size:       2645    1003     332     256    3897     396

  Fragments arranged by size: 

                3897    2645    1003     396     332     256
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3323    3358    8529 
   Size:       3323      35    5171

  Fragments arranged by size: 

                5171    3323      35
 
DraI TTT'AAA

Cuts at:      0    2891    3542    5396    8529 
   Size:       2891     651    1854    3133

  Fragments arranged by size: 

                3133    2891    1854     651
 
DraIII CAC_nnn'GTG

Cuts at:      0    8320    8529 
   Size:       8320     209
 
EaeI y'GGCC_r

Cuts at:      0    6972    8401    8529 
   Size:       6972    1429     128

  Fragments arranged by size: 

                6972    1429     128
 
EarI CTCTTCn'nnn_

Cuts at:      0     354    1373    2258    2508    3461    3497    8529 
   Size:        354    1019     885     250     953      36    5032

  Fragments arranged by size: 

                5032    1019     953     885     354     250      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     269     341     469    2981    3464    3869    3968 
   Size:         67     202      72     128    2512     483     405      99

Cuts at:   3968    4013    4049    4472    4562    5033    5764    6122    6749 
   Size:         45      36     423      90     471     731     358     627

Cuts at:   6749    7088    7427    8378    8405    8529 
   Size:        339     339     951      27     124

  Fragments arranged by size: 

                2512     951     731     627     483     471     423     405
                 358     339     339     202     128     124      99      90
                  72      67      45      36      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    5075    5546    6115    6785    8529 
   Size:       5075     471     569     670    1744

  Fragments arranged by size: 

                5075    1744     670     569     471
 
EcoO109I rG'GnC_Cy

Cuts at:      0    4683    7427    8529 
   Size:       4683    2744    1102

  Fragments arranged by size: 

                4683    2744    1102
 
EcoRI G'AATT_C

Cuts at:      0     659    6497    8529 
   Size:        659    5838    2032

  Fragments arranged by size: 

                5838    2032     659
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3236    3398    5531    7727    8529 
   Size:       3236     162    2133    2196     802

  Fragments arranged by size: 

                3236    2196    2133     802     162
 
HaeII r_GCGC'y

Cuts at:      0    7575    8499    8529 
   Size:       7575     924      30

  Fragments arranged by size: 

                7575     924      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     718     750     903     935     973    1005    1081    1113 
   Size:        718      32     153      32      38      32      76      32

Cuts at:   1113    2413    2445    3637    3669    3701    7604    7636    8403 
   Size:       1300      32    1192      32      32    3903      32     767

Cuts at:   8403    8435    8529 
   Size:         32      94

  Fragments arranged by size: 

                3903    1300    1192     767     718     153      94      76
                  38      32      32      32      32      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:      0    5616    7322    8529 
   Size:       5616    1706    1207

  Fragments arranged by size: 

                5616    1706    1207
 
HindIII A'AGCT_T

Cuts at:      0    2241    2517    6380    8529 
   Size:       2241     276    3863    2149

  Fragments arranged by size: 

                3863    2241    2149     276
 
KpnI G_GTAC'C

Cuts at:      0     617    8529 
   Size:        617    7912
 
MfeI C'AATT_G

Cuts at:      0     562    8529 
   Size:        562    7967
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1677    2798    3291    5729    8529 
   Size:       1677    1121     493    2438    2800

  Fragments arranged by size: 

                2800    2438    1677    1121     493
 
MslI CAynn'nnrTG

Cuts at:      0    1085    1388    3041    8529 
   Size:       1085     303    1653    5488

  Fragments arranged by size: 

                5488    1653    1085     303
 
MspA1I CmG'CkG

Cuts at:      0    1028    3847    3946    4833    8529 
   Size:       1028    2819      99     887    3696

  Fragments arranged by size: 

                3696    2819    1028     887      99
 
NdeI CA'TA_TG

Cuts at:      0      15    3038    3629    8529 
   Size:         15    3023     591    4900

  Fragments arranged by size: 

                4900    3023     591      15
 
NheI G'CTAG_C

Cuts at:      0    1002    8529 
   Size:       1002    7527
 
NruI TCG'CGA

Cuts at:      0     993    8529 
   Size:        993    7536
 
NsiI A_TGCA'T

Cuts at:      0    2040    5182    8529 
   Size:       2040    3142    3347

  Fragments arranged by size: 

                3347    3142    2040
 
NspI r_CATG'y

Cuts at:      0    1393    2042    2118    2840    4888    6368    6663    7774 
   Size:       1393     649      76     722    2048    1480     295    1111

Cuts at:   7774    8529 
   Size:        755

  Fragments arranged by size: 

                2048    1480    1393    1111     755     722     649     295
                  76
 
PciI A'CATG_T

Cuts at:      0    2836    7770    8529 
   Size:       2836    4934     759

  Fragments arranged by size: 

                4934    2836     759
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     627    3277    4231    8529 
   Size:        627    2650     954    4298

  Fragments arranged by size: 

                4298    2650     954     627
 
PfoI T'CCnGG_A

Cuts at:      0    2607    6699    8529 
   Size:       2607    4092    1830

  Fragments arranged by size: 

                4092    2607    1830
 
PmlI CAC'GTG

Cuts at:      0    5362    8529 
   Size:       5362    3167
 
PpuMI rG'GwC_Cy

Cuts at:      0    4683    7427    8529 
   Size:       4683    2744    1102

  Fragments arranged by size: 

                4683    2744    1102
 
PshAI GACnn'nnGTC

Cuts at:      0    2026    8529 
   Size:       2026    6503
 
PsiI TTA'TAA

Cuts at:      0    4252    6584    8529 
   Size:       4252    2332    1945

  Fragments arranged by size: 

                4252    2332    1945
 
PspXI vC'TCGA_Gb

Cuts at:      0    7676    8529 
   Size:       7676     853
 
PstI C_TGCA'G

Cuts at:      0       9    2172    2652    4013    4413    4425    8529 
   Size:          9    2163     480    1361     400      12    4104

  Fragments arranged by size: 

                4104    2163    1361     480     400      12       9
 
PvuII CAG'CTG

Cuts at:      0    1028    3847    3946    4833    8529 
   Size:       1028    2819      99     887    3696

  Fragments arranged by size: 

                3696    2819    1028     887      99
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1373    8529 
   Size:       1373    7156
 
ScaI AGT'ACT

Cuts at:      0     140     390    4485    7056    7875    8529 
   Size:        140     250    4095    2571     819     654

  Fragments arranged by size: 

                4095    2571     819     654     250     140
 
SexAI A'CCwGG_T

Cuts at:      0    5243    8529 
   Size:       5243    3286
 
SfcI C'TryA_G

Cuts at:      0       5    1414    1476    1823    2168    2483    2648    4009 
   Size:          5    1409      62     347     345     315     165    1361

Cuts at:   4009    4409    4421    5072    7541    8264    8529 
   Size:        400      12     651    2469     723     265

  Fragments arranged by size: 

                2469    1409    1361     723     651     400     347     345
                 315     265     165      62      12       5
 
SmaI CCC'GGG

Cuts at:      0    1120    8529 
   Size:       1120    7409
 
SmlI C'TyrA_G

Cuts at:      0     409    2119    2238    3259    5099    6005    6063    6166 
   Size:        409    1710     119    1021    1840     906      58     103

Cuts at:   6166    6674    6720    6757    7353    7676    8298    8529 
   Size:        508      46      37     596     323     622     231

  Fragments arranged by size: 

                1840    1710    1021     906     622     596     508     409
                 323     231     119     103      58      46      37
 
SphI G_CATG'C

Cuts at:      0    2118    8529 
   Size:       2118    6411
 
SspI AAT'ATT

Cuts at:      0    2223    3091    8529 
   Size:       2223     868    5438

  Fragments arranged by size: 

                5438    2223     868
 
StuI AGG'CCT

Cuts at:      0    2199    8529 
   Size:       2199    6330
 
StyI C'CwwG_G

Cuts at:      0     929    2342    5459    7036    8368    8529 
   Size:        929    1413    3117    1577    1332     161

  Fragments arranged by size: 

                3117    1577    1413    1332     929     161
 
SwaI ATTT'AAAT

Cuts at:      0    5396    8529 
   Size:       5396    3133
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    4119    5292    6193    7962    8529 
   Size:       4119    1173     901    1769     567

  Fragments arranged by size: 

                4119    1769    1173     901     567
 
TatI w'GTAC_w

Cuts at:      0     138     388    2207    3214    4159    4483    4841    5086 
   Size:        138     250    1819    1007     945     324     358     245

Cuts at:   5086    6520    6724    7054    7873    8529 
   Size:       1434     204     330     819     656

  Fragments arranged by size: 

                1819    1434    1007     945     819     656     358     330
                 324     250     245     204     138
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     917     949    3543    3575    6792    6824    8529 
   Size:        917      32    2594      32    3217      32    1705

  Fragments arranged by size: 

                3217    2594    1705     917      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    5245    8529 
   Size:       5245    3284
 
XbaI T'CTAG_A

Cuts at:      0    5592    6373    6898    6910    7249    7405    7609    8529 
   Size:       5592     781     525      12     339     156     204     920

  Fragments arranged by size: 

                5592     920     781     525     339     204     156      12
            
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     561     936    1078    3274    5133    8142    8529 
   Size:        561     375     142    2196    1859    3009     387

  Fragments arranged by size: 

                3009    2196    1859     561     387     375     142
 
XhoI C'TCGA_G

Cuts at:      0    6674    7676    8529 
   Size:       6674    1002     853

  Fragments arranged by size: 

                6674    1002     853
 
XmaI C'CCGG_G

Cuts at:      0    1118    8529 
   Size:       1118    7411
 
XmnI GAAnn'nnTTC

Cuts at:      0    2616    5577    6072    7257    8529 
   Size:       2616    2961     495    1185    1272

  Fragments arranged by size: 

                2961    2616    1272    1185     495
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AlfI
     AloI    AlwNI    ApaLI     ApoI     AseI     AvaI    AvrII     BaeI
     BanI    BanII     BbsI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI    CspCI     DraI   DraIII     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII
     KpnI     MfeI     MmeI     MslI   MspA1I     NdeI     NheI     NruI
     NsiI     NspI     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI    PspXI     PstI    PvuII     SapI     ScaI    SexAI     SfcI
     SmaI     SmlI     SphI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AgeI     AhdI     AleI     ApaI     AscI    AsiSI
    BamHI     BbeI    BbvCI     BcgI    BmgBI    BsaHI    BsiEI    BsiWI
    BsmBI    BspEI   BssHII    BssSI   BstEII     ClaI     DrdI     EagI
     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI     HpaI     KasI
     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NotI     PacI
     PasI     PmeI     PpiI   PspOMI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     ZraI
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