Restriction map for
pFN21AB0006
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# NO CUT ENZYMES ( 67 enzymes) #
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AatII Acc65I AccII AccIII AclI
AgeI AocI Aor51HI AscI Asp700I
Asp718I AspI AviII BbeI BbvCI
BsaBI BseAI BsiWI BspDI BspEI
BssHII BstUI Bsu36I ClaI CpoI
DraIII Eco47III Eco81I FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflII 5113 CTTAAG C^TTAAG
AseI 4606 ATTAAT AT^TAAT
AsnI 4606 ATTAAT AT^TAAT
AvrII 207 CCTAGG C^CTAGG
BamHI 4166 GGATCC G^GATCC
BfrI 5113 CTTAAG C^TTAAG
BglI 6608 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 207 CCTAGG C^CTAGG
BlpI 97 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 97 GCTCAGC GC^T[ACGT]AGC
BsaWI 5014 ACCGGA [AT]^CCGG[AT]
BsiEI 789 CGGCCG CG[GA][CT]^CG
BsmI 1890 GAATGC GAATGC(1/-1)
Bst1107I 2026 GTATAC GTA^TAC
BstBI 1167 TTCGAA TT^CGAA
BstZ17I 2026 GTATAC GTA^TAC
CelII 97 GCTCAGC GC^T[ACGT]AGC
DraI 6322 TTTAAA TTT^AAA
EagI 789 CGGCCG C^GGCCG
EclXI 789 CGGCCG C^GGCCG
Eco52I 789 CGGCCG C^GGCCG
EcoT22I 1521 ATGCAT ATGCA^T
NsiI 1521 ATGCAT ATGCA^T
NspV 1167 TTCGAA TT^CGAA
PshAI 1465 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4606 ATTAAT AT^TAAT
SalI 1672 GTCGAC G^TCGAC
SexAI 2613 ACCTGGT A^CC[AT]GGT
SfuI 1167 TTCGAA TT^CGAA
SmaI 19 CCCGGG CCC^GGG
XmaCI 19 CCCGGG C^CCGGG
XmaI 19 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 3321 GACGCC G[GA]^CG[CT]C
5422 GACGCC
AhdI 1854 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2233 GACACCGTGTC
Alw44I 1485 GTGCAC G^TGCAC
4126 GTGCAC
ApaLI 1485 GTGCAC G^TGCAC
4126 GTGCAC
AspEI 1854 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2233 GACACCGTGTC
BciVI 3076 GGATAC GTATCC(6/5)
3190 GTATCC
BglII 5369 AGATCT A^GATCT
5756 AGATCT
BsaHI 3321 GACGCC G[GA]^CG[CT]C
5422 GACGCC
BsmBI 2140 GAGACG CGTCTC(1/5)
5245 GAGACG
BspHI 1602 TCATGA T^CATGA
6507 TCATGA
BspLU11I 488 ACATGT A^CATGT
6466 ACATGT
BsrBI 196 GAGCGG CCGCTC(-3/-3)
840 GAGCGG
BsrFI 2803 ACCGGC [GA]^CCGG[CT]
5521 ACCGGC
BstAPI 665 GCATCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2853 GCAGAGGCTGC
Cfr10I 2803 ACCGGC [GA]^CCGG[CT]
5521 ACCGGC
DrdI 2758 GACGTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3513 GACACCATTGTC
Eam1105I 1854 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2233 GACACCGTGTC
EcoRI 4230 GAATTC G^AATTC
6490 GAATTC
EcoRV 2376 GATATC GAT^ATC
4982 GATATC
Hin1I 3321 GACGCC G[GA]^CG[CT]C
5422 GACGCC
NcoI 1385 CCATGG C^CATGG
2943 CCATGG
RcaI 1602 TCATGA T^CATGA
6507 TCATGA
SapI 81 GAAGAGC GCTCTTC(1/4)
5670 GAAGAGC
StuI 2007 AGGCCT AGG^CCT
6117 AGGCCT
XbaI 679 TCTAGA T^CTAGA
6379 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BcgI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII