(Circular) (Six-base) MAPSORT of: pFN21AB0006.seq Check: 7626 from: 1 to: 11500 pFN21AB0006 11500 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3648 3648 Size: 11500 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9310 278 Size: 53 83 186 936 7774 2468 Fragments arranged by size: 7774 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2949 3678 4032 4415 5454 6027 6506 8843 Size: 1012 729 354 383 1039 573 479 2337 Cuts at: 8843 1937 Size: 4594 Fragments arranged by size: 4594 2337 1039 1012 729 573 479 383 354 Acc65I G'GTAC_C Cuts at: 8821 8821 Size: 11500 AclI AA'CG_TT Cuts at: 1563 9627 10000 1563 Size: 8064 373 3063 Fragments arranged by size: 8064 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2298 2304 2993 4037 5385 5943 6178 6938 Size: 666 6 689 1044 1348 558 235 760 Cuts at: 6938 7682 9563 10947 11215 1632 Size: 744 1881 1384 268 1917 Fragments arranged by size: 1917 1881 1384 1348 1044 760 744 689 666 558 268 235 6 AflII C'TTAA_G Cuts at: 820 1017 7118 820 Size: 197 6101 5202 Fragments arranged by size: 6101 5202 197 AflIII A'CryG_T Cuts at: 1203 2493 3494 8471 8522 10399 11376 1203 Size: 1290 1001 4977 51 1877 977 1327 Fragments arranged by size: 4977 1877 1327 1290 1001 977 51 AgeI A'CCGG_T Cuts at: 10331 10331 Size: 11500 AhdI GACnn_n'nnGTC Cuts at: 3864 4243 10229 3864 Size: 379 5986 5135 Fragments arranged by size: 5986 5135 379 AleI CACnn'nnGTG Cuts at: 1166 2828 4118 4325 5294 5312 5570 6195 1166 Size: 1662 1290 207 969 18 258 625 6471 Fragments arranged by size: 6471 1662 1290 969 625 258 207 18 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2440 6156 1184 Size: 1256 3716 6528 Fragments arranged by size: 6528 3716 1256 AlwNI CAG_nnn'CTG Cuts at: 2053 2971 3701 4300 4436 4864 6347 6874 7364 Size: 918 730 599 136 428 1483 527 490 Cuts at: 7364 9243 10815 2053 Size: 1879 1572 2738 Fragments arranged by size: 2738 1879 1572 1483 918 730 599 527 490 428 136 ApaI G_GGCC'C Cuts at: 2025 5071 8014 2025 Size: 3046 2943 5511 Fragments arranged by size: 5511 3046 2943 ApaLI G'TGCA_C Cuts at: 3490 6131 9557 10713 3490 Size: 2641 3426 1156 4277 Fragments arranged by size: 4277 3426 2641 1156 ApoI r'AATT_y Cuts at: 1493 3244 3949 5659 6235 8495 8809 9035 9187 Size: 1751 705 1710 576 2260 314 226 152 Cuts at: 9187 11296 1493 Size: 2109 1697 Fragments arranged by size: 2260 2109 1751 1710 1697 705 576 314 226 152 AseI AT'TA_AT Cuts at: 160 1032 6612 10054 160 Size: 872 5580 3442 1606 Fragments arranged by size: 5580 3442 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11500 AvaI C'yCGr_G Cuts at: 1870 1943 2024 3234 5064 6147 6185 6207 8793 Size: 73 81 1210 1830 1083 38 22 2586 Cuts at: 8793 8825 1870 Size: 32 4545 Fragments arranged by size: 4545 2586 1830 1210 1083 81 73 38 32 22 AvrII C'CTAG_G Cuts at: 2212 2212 Size: 11500 BamHI G'GATC_C Cuts at: 6171 8830 9197 6171 Size: 2659 367 8474 Fragments arranged by size: 8474 2659 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 3254 4703 5383 6220 Size: 325 217 627 278 1189 1449 680 837 Cuts at: 6220 8135 8391 8582 8821 10276 618 Size: 1915 256 191 239 1455 1842 Fragments arranged by size: 1915 1842 1455 1449 1189 837 680 627 325 278 256 239 217 191 BanII G_rGCy'C Cuts at: 1391 2025 5071 5231 5273 5696 5869 7195 7412 Size: 634 3046 160 42 423 173 1326 217 Cuts at: 7412 7686 8014 8819 1391 Size: 274 328 805 4072 Fragments arranged by size: 4072 3046 1326 805 634 423 328 274 217 173 160 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2981 6067 7022 8546 928 Size: 971 1082 3086 955 1524 3882 Fragments arranged by size: 3882 3086 1524 1082 971 955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2855 2889 3537 3571 8171 Size: 34 50 34 834 34 648 34 4600 Cuts at: 8171 8205 9691 9725 1903 Size: 34 1486 34 3678 Fragments arranged by size: 4600 3678 1486 834 648 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5075 5206 9392 10602 5075 Size: 131 4186 1210 5973 Fragments arranged by size: 5973 4186 1210 131 BclI T'GATC_A Cuts at: 1548 2566 5316 5686 6399 1548 Size: 1018 2750 370 713 6649 Fragments arranged by size: 6649 2750 1018 713 370 BfrBI ATG'CAT Cuts at: 3528 3528 Size: 11500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8619 10111 136 Size: 107 122 71 631 605 6947 1492 1525 Fragments arranged by size: 6947 1525 1492 631 605 122 107 71 BglII A'GATC_T Cuts at: 7374 7761 11495 7374 Size: 387 3734 7379 Fragments arranged by size: 7379 3734 387 BlpI GC'TnA_GC Cuts at: 2103 8917 2103 Size: 6814 4686 Bme1580I G_kGCm'C Cuts at: 1790 2025 2070 3494 3621 4728 5071 6135 6225 Size: 235 45 1424 127 1107 343 1064 90 Cuts at: 6225 8014 9561 10717 1790 Size: 1789 1547 1156 2573 Fragments arranged by size: 2573 1789 1547 1424 1156 1107 1064 343 235 127 90 45 BmgBI CAC'GTC Cuts at: 1318 4765 1318 Size: 3447 8053 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2116 2319 3711 Size: 416 53 83 399 24 699 203 1392 Cuts at: 3711 6949 7860 8655 9231 10189 442 Size: 3238 911 795 576 958 1753 Fragments arranged by size: 3238 1753 1392 958 911 795 699 576 416 399 203 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11500 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2118 4983 5073 6066 6888 2118 Size: 2865 90 993 822 6730 Fragments arranged by size: 6730 2865 993 822 90 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2485 2756 2761 4516 4997 5262 5421 5570 Size: 1246 271 5 1755 481 265 159 149 Cuts at: 5570 5589 5747 6004 6246 6555 7187 7255 10160 Size: 19 158 257 242 309 632 68 2905 Cuts at: 10160 1239 Size: 2579 Fragments arranged by size: 2905 2579 1755 1246 632 481 309 271 265 257 242 159 158 149 68 19 5 Bpu10I CC'TnA_GC Cuts at: 2009 6947 7146 8347 2009 Size: 4938 199 1201 5162 Fragments arranged by size: 5162 4938 1201 199 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 9625 10490 10788 3811 Size: 5814 865 298 4523 Fragments arranged by size: 5814 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2464 3934 4222 4231 5297 6196 7208 Size: 617 965 1470 288 9 1066 899 1012 Cuts at: 7208 7563 10163 882 Size: 355 2600 2219 Fragments arranged by size: 2600 2219 1470 1066 1012 965 899 617 355 288 9 BsaAI yAC'GTr Cuts at: 493 1204 3006 3495 7514 8551 9291 493 Size: 711 1802 489 4019 1037 740 2702 Fragments arranged by size: 4019 2702 1802 1037 740 711 489 BsaBI GATnn'nnATC Cuts at: 11494 11494 Size: 11500 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5327 7428 9307 9689 Size: 53 83 186 936 3794 2101 1879 382 Cuts at: 9689 10324 275 Size: 635 1451 Fragments arranged by size: 3794 2101 1879 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7019 9933 10331 10605 10752 11163 7019 Size: 2914 398 274 147 411 7356 Fragments arranged by size: 7356 2914 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3844 3874 3980 4010 5317 Size: 30 165 30 2441 30 106 30 1307 Cuts at: 5317 5347 5851 5881 6718 6748 1178 Size: 30 504 30 837 30 5930 Fragments arranged by size: 5930 2441 1307 837 504 165 106 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2482 2814 2901 3252 4152 4292 5391 5608 Size: 1296 332 87 351 900 140 1099 217 Cuts at: 5608 5799 6135 7551 1186 Size: 191 336 1416 5135 Fragments arranged by size: 5135 1416 1296 1099 900 351 336 332 217 191 140 87 BseYI C'CCAG_C Cuts at: 2387 2576 2904 5353 5797 6405 10703 11199 2387 Size: 189 328 2449 444 608 4298 496 2688 Fragments arranged by size: 4298 2688 2449 608 496 444 328 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2767 2852 2895 3109 3181 3190 3397 Size: 573 1031 85 43 214 72 9 207 Cuts at: 3397 4821 5683 6888 8452 8581 1163 Size: 1424 862 1205 1564 129 4082 Fragments arranged by size: 4082 1564 1424 1205 1031 862 573 214 207 129 85 72 43 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2797 8950 9711 10739 1540 Size: 275 186 796 6153 761 1028 2301 Fragments arranged by size: 6153 2301 1028 796 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3494 4854 5273 5577 5600 5696 6047 6135 6192 Size: 1360 419 304 23 96 351 88 57 Cuts at: 6192 7412 9561 9646 10717 3494 Size: 1220 2149 85 1071 4277 Fragments arranged by size: 4277 2149 1360 1220 1071 419 351 304 96 88 85 57 23 BsmI GAATG_Cn' Cuts at: 3901 9011 9104 3901 Size: 5110 93 6297 Fragments arranged by size: 6297 5110 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4139 7244 4139 Size: 3105 8395 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2070 3494 3621 4728 4854 5071 Size: 399 235 45 1424 127 1107 126 217 Cuts at: 5071 5231 5273 5577 5600 5696 5869 6047 6135 Size: 160 42 304 23 96 173 178 88 Cuts at: 6135 6192 6225 7195 7412 7686 8014 8819 9561 Size: 57 33 970 217 274 328 805 742 Cuts at: 9561 9646 10717 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1424 1107 1071 970 805 742 399 328 304 274 235 217 217 178 173 160 127 126 96 88 85 57 45 42 33 23 BspEI T'CCGG_A Cuts at: 11163 11163 Size: 11500 BspHI T'CATG_A Cuts at: 3607 8512 9389 11119 3607 Size: 4905 877 1730 3988 Fragments arranged by size: 4905 3988 1730 877 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2384 2546 2775 3648 4350 4749 4848 5829 Size: 1540 162 229 873 702 399 99 981 Cuts at: 5829 6764 7467 8855 844 Size: 935 703 1388 3489 Fragments arranged by size: 3489 1540 1388 981 935 873 703 702 399 229 162 99 BsrBI CCG'CTC Cuts at: 1394 1823 2203 2847 9387 1394 Size: 429 380 644 6540 3507 Fragments arranged by size: 6540 3507 644 429 380 BsrDI GCAATG_nn' Cuts at: 65 1248 2224 3528 3796 7653 9995 10169 65 Size: 1183 976 1304 268 3857 2342 174 1396 Fragments arranged by size: 3857 2342 1396 1304 1183 976 268 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4808 7526 10144 10331 1700 Size: 110 2998 2718 2618 187 2869 Fragments arranged by size: 2998 2869 2718 2618 187 110 BsrGI T'GTAC_A Cuts at: 96 3632 7383 8770 96 Size: 3536 3751 1387 2826 Fragments arranged by size: 3751 3536 2826 1387 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11500 BssSI C'ACGA_G Cuts at: 3401 5201 5388 7194 8254 9560 10572 3401 Size: 1800 187 1806 1060 1306 1012 4329 Fragments arranged by size: 4329 1806 1800 1306 1060 1012 187 BstAPI GCAn_nnn'nTGC Cuts at: 794 2676 4864 794 Size: 1882 2188 7430 Fragments arranged by size: 7430 2188 1882 BstBI TT'CG_AA Cuts at: 3173 10304 3173 Size: 7131 4369 BstEII G'GTnAC_C Cuts at: 1184 4771 4915 7000 7726 1184 Size: 3587 144 2085 726 4958 Fragments arranged by size: 4958 3587 2085 726 144 BstXI CCAn_nnnn'nTGG Cuts at: 3377 5899 7573 8402 3377 Size: 2522 1674 829 6475 Fragments arranged by size: 6475 2522 1674 829 BstYI r'GATC_y Cuts at: 1972 2849 4303 6171 7374 7761 8830 9197 9582 Size: 877 1454 1868 1203 387 1069 367 385 Cuts at: 9582 9599 11051 11137 11149 11495 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1868 1454 1452 1203 1069 877 387 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4033 4033 Size: 11500 BtgI C'CryG_G Cuts at: 513 1065 2073 3390 3778 4948 5094 6574 7062 Size: 552 1008 1317 388 1170 146 1480 488 Cuts at: 7062 8208 513 Size: 1146 3805 Fragments arranged by size: 3805 1480 1317 1170 1146 1008 552 488 388 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2398 487 Size: 683 56 1172 9589 Fragments arranged by size: 9589 1172 683 56 BtsI GCAGTG_nn' Cuts at: 2972 4809 4947 5255 5976 7434 9016 9810 9830 Size: 1837 138 308 721 1458 1582 794 20 Cuts at: 9830 2972 Size: 4642 Fragments arranged by size: 4642 1837 1582 1458 794 721 308 138 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11500 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 7423 7458 529 Size: 35 6859 35 4571 Fragments arranged by size: 6859 4571 35 35 DraI TTT'AAA Cuts at: 8329 8804 8329 Size: 475 11025 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4769 5524 10507 809 Size: 507 3453 755 4983 1802 Fragments arranged by size: 4983 3453 1802 755 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2794 2920 Size: 54 1425 50 275 18 210 754 126 Cuts at: 2920 3775 4066 4170 4351 5205 5647 6328 6571 Size: 855 291 104 181 854 442 681 243 Cuts at: 6571 7086 7291 7470 7929 8452 8680 8947 9836 Size: 515 205 179 459 523 228 267 889 Cuts at: 9836 11465 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 855 854 754 681 523 515 459 442 291 275 267 243 228 210 205 181 179 126 104 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2794 8947 1537 Size: 275 982 6153 4090 Fragments arranged by size: 6153 4090 982 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2280 3316 3606 5104 7670 8190 9430 Size: 728 199 1036 290 1498 2566 520 1240 Cuts at: 9430 1353 Size: 3423 Fragments arranged by size: 3423 2566 1498 1240 1036 728 520 290 199 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6164 7924 10087 10461 10607 387 Size: 1064 138 4575 1760 2163 374 146 1280 Fragments arranged by size: 4575 2163 1760 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5271 5694 7410 5271 Size: 423 1716 9361 Fragments arranged by size: 9361 1716 423 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2298 2304 2485 2756 2761 2993 4037 Size: 393 666 6 181 271 5 232 1044 Cuts at: 4037 4516 4997 5262 5385 5421 5570 5589 5747 Size: 479 481 265 123 36 149 19 158 Cuts at: 5747 5943 6004 6178 6246 6555 6938 7187 7255 Size: 196 61 174 68 309 383 249 68 Cuts at: 7255 7682 9563 10160 10947 11215 1239 Size: 427 1881 597 787 268 1524 Fragments arranged by size: 1881 1524 1044 787 666 597 481 479 427 393 383 309 271 268 265 249 232 196 181 174 158 149 123 68 68 61 36 19 6 5 EcoNI CCTnn'n_nnAGG Cuts at: 4732 5590 6054 4732 Size: 858 464 10178 Fragments arranged by size: 10178 858 464 EcoO109I rG'GnC_Cy Cuts at: 2021 4738 5138 6361 8011 2021 Size: 2717 400 1223 1650 5510 Fragments arranged by size: 5510 2717 1650 1223 400 EcoRI G'AATT_C Cuts at: 6235 8495 8809 9187 6235 Size: 2260 314 378 8548 Fragments arranged by size: 8548 2260 378 314 EcoRV GAT'ATC Cuts at: 4383 6989 4383 Size: 2606 8894 FspI TGC'GCA Cuts at: 10006 10006 Size: 11500 HaeII r_GCGC'y Cuts at: 1678 4436 6954 8629 10647 1678 Size: 2758 2518 1675 2018 2531 Fragments arranged by size: 2758 2531 2518 2018 1675 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3697 3729 3850 3882 4229 Size: 32 135 32 2125 32 121 32 347 Cuts at: 4229 4261 4463 4495 5047 5079 5317 5349 5678 Size: 32 202 32 552 32 238 32 329 Cuts at: 5678 5710 6130 6141 6162 6163 6173 6195 6623 Size: 32 420 11 21 1 10 22 428 Cuts at: 6623 6655 7043 7075 7436 7468 7560 7592 7746 Size: 32 388 32 361 32 92 32 154 Cuts at: 7746 7778 8698 8730 10141 10173 10215 10247 1373 Size: 32 920 32 1411 32 42 32 2626 Fragments arranged by size: 2626 2125 1411 920 552 428 420 388 361 347 329 238 202 154 135 121 92 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 22 21 11 10 1 HincII GTy'rAC Cuts at: 669 1657 1938 3679 4837 8224 8844 9090 669 Size: 988 281 1741 1158 3387 620 246 3079 Fragments arranged by size: 3387 3079 1741 1158 988 620 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 11500 HpaI GTT'AAC Cuts at: 9090 9090 Size: 11500 KpnI G_GTAC'C Cuts at: 8825 8825 Size: 11500 MfeI C'AATT_G Cuts at: 4482 5499 8745 9099 4482 Size: 1017 3246 354 6883 Fragments arranged by size: 6883 3246 1017 354 MluI A'CGCG_T Cuts at: 11376 11376 Size: 11500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2575 3944 4260 7479 8272 10614 10798 1305 Size: 1270 1369 316 3219 793 2342 184 2007 Fragments arranged by size: 3219 2342 2007 1369 1270 793 316 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2922 4172 5207 6330 7088 Size: 54 1425 343 1090 1250 1035 1123 758 Cuts at: 7088 7293 7472 8454 8682 10 Size: 205 179 982 228 2828 Fragments arranged by size: 2828 1425 1250 1123 1090 1035 982 758 343 228 205 179 54 MslI CAynn'nnrTG Cuts at: 518 1166 2828 4118 4325 4778 5294 5312 5420 Size: 648 1662 1290 207 453 516 18 108 Cuts at: 5420 5570 6195 6554 8153 8386 8400 9458 9817 Size: 150 625 359 1599 233 14 1058 359 Cuts at: 9817 9976 518 Size: 159 2042 Fragments arranged by size: 2042 1662 1599 1290 1058 648 625 516 453 359 359 233 207 159 150 108 18 14 MspA1I CmG'CkG Cuts at: 1606 2063 2664 2879 3027 3041 5801 6405 6522 Size: 457 601 215 148 14 2760 604 117 Cuts at: 6522 8617 8916 9593 10741 10986 1606 Size: 2095 299 677 1148 245 2120 Fragments arranged by size: 2760 2120 2095 1148 677 604 601 457 299 245 215 148 117 14 NcoI C'CATG_G Cuts at: 513 1065 3390 4948 513 Size: 552 2325 1558 7065 Fragments arranged by size: 7065 2325 1558 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 11500 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11500 NotI GC'GGCC_GC Cuts at: 8947 8947 Size: 11500 NsiI A_TGCA'T Cuts at: 3530 3530 Size: 11500 NspI r_CATG'y Cuts at: 1132 1229 2497 3528 3625 3639 8475 8604 8858 Size: 97 1268 1031 97 14 4836 129 254 Cuts at: 8858 10403 1132 Size: 1545 2229 Fragments arranged by size: 4836 2229 1545 1268 1031 254 129 97 97 14 PasI CC'CwG_GG Cuts at: 1348 4734 4910 7811 1348 Size: 3386 176 2901 5037 Fragments arranged by size: 5037 3386 2901 176 PciI A'CATG_T Cuts at: 2493 8471 10399 2493 Size: 5978 1928 3594 Fragments arranged by size: 5978 3594 1928 PflMI CCAn_nnn'nTGG Cuts at: 1108 2053 2364 3891 4782 5342 7233 7289 8007 Size: 945 311 1527 891 560 1891 56 718 Cuts at: 8007 1108 Size: 4601 Fragments arranged by size: 4601 1891 1527 945 891 718 560 311 56 PfoI T'CCnGG_A Cuts at: 5437 6477 8019 8092 5437 Size: 1040 1542 73 8845 Fragments arranged by size: 8845 1542 1040 73 PmeI GTTT'AAAC Cuts at: 8804 8804 Size: 11500 PmlI CAC'GTG Cuts at: 3006 3495 7514 3006 Size: 489 4019 6992 Fragments arranged by size: 6992 4019 489 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9569 9601 11082 11114 9569 Size: 32 1481 32 9955 Fragments arranged by size: 9955 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4738 5138 6361 4738 Size: 400 1223 9877 Fragments arranged by size: 9877 1223 400 PshAI GACnn'nnGTC Cuts at: 1613 3474 1613 Size: 1861 9639 PsiI TTA'TAA Cuts at: 9070 9070 Size: 11500 PspOMI G'GGCC_C Cuts at: 2021 5067 8010 2021 Size: 3046 2943 5511 Fragments arranged by size: 5511 3046 2943 PspXI vC'TCGA_Gb Cuts at: 3234 6147 3234 Size: 2913 8587 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 7624 7656 7624 Size: 32 11468 PstI C_TGCA'G Cuts at: 830 4746 5175 5262 6775 6889 8852 830 Size: 3916 429 87 1513 114 1963 3478 Fragments arranged by size: 3916 3478 1963 1513 429 114 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11500 PvuII CAG'CTG Cuts at: 2879 3027 3041 5801 6405 2879 Size: 148 14 2760 604 7974 Fragments arranged by size: 7974 2760 604 148 14 SacI G_AGCT'C Cuts at: 5273 5696 7412 5273 Size: 423 1716 9361 Fragments arranged by size: 9361 1716 423 SalI G'TCGA_C Cuts at: 1936 3677 8842 1936 Size: 1741 5165 4594 Fragments arranged by size: 5165 4594 1741 SanDI GG'GwC_CC Cuts at: 5138 5138 Size: 11500 SapI GCTCTTCn'nnn_ Cuts at: 2081 7670 2081 Size: 5589 5911 SbfI CC_TGCA'GG Cuts at: 8852 8852 Size: 11500 ScaI AGT'ACT Cuts at: 9748 11460 9748 Size: 1712 9788 SexAI A'CCwGG_T Cuts at: 4618 4618 Size: 11500 SfcI C'TryA_G Cuts at: 826 1045 2722 3954 4742 5171 5258 6166 6507 Size: 219 1677 1232 788 429 87 908 341 Cuts at: 6507 6771 6885 8848 9983 10664 10855 826 Size: 264 114 1963 1135 681 191 1471 Fragments arranged by size: 1963 1677 1471 1232 1135 908 788 681 429 341 264 219 191 114 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11500 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11500 SmaI CCC'GGG Cuts at: 1872 2026 8827 1872 Size: 154 6801 4545 Fragments arranged by size: 6801 4545 154 SmlI C'TyrA_G Cuts at: 820 1017 1943 3234 3826 6147 6207 7118 9604 Size: 197 926 1291 592 2321 60 911 2486 Cuts at: 9604 10505 10767 820 Size: 901 262 1553 Fragments arranged by size: 2486 2321 1553 1291 926 911 901 592 262 197 60 SnaBI TAC'GTA Cuts at: 493 9291 493 Size: 8798 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11500 SphI G_CATG'C Cuts at: 1132 8858 1132 Size: 7726 3774 SrfI GCCC'GGGC Cuts at: 2026 2026 Size: 11500 SspI AAT'ATT Cuts at: 5 52 9424 5 Size: 47 9372 2081 Fragments arranged by size: 9372 2081 47 StuI AGG'CCT Cuts at: 1517 4014 8124 1517 Size: 2497 4110 4893 Fragments arranged by size: 4893 4110 2497 StyI C'CwwG_G Cuts at: 513 1065 2212 2620 3390 4948 7008 8939 513 Size: 552 1147 408 770 1558 2060 1931 3074 Fragments arranged by size: 3074 2060 1931 1558 1147 770 552 408 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 3632 4205 5345 6671 6952 6982 Size: 1255 738 1314 573 1140 1326 281 30 Cuts at: 6982 7495 7820 9527 9544 9697 9882 10327 325 Size: 513 325 1707 17 153 185 445 1498 Fragments arranged by size: 1707 1498 1326 1314 1255 1140 738 573 513 445 325 281 185 153 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2231 2304 2642 Size: 274 80 33 51 1443 254 73 338 Cuts at: 2642 3632 3924 4670 5127 5187 5730 7383 8034 Size: 990 292 746 457 60 543 1653 651 Cuts at: 8034 8770 9746 11458 96 Size: 736 976 1712 138 Fragments arranged by size: 1712 1653 1443 990 976 746 736 651 543 457 338 292 274 254 138 80 73 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3496 3528 3844 3876 3980 4012 4242 4274 5851 Size: 32 316 32 104 32 230 32 1577 Cuts at: 5851 5883 6978 7010 7068 7100 8240 8272 11460 Size: 32 1095 32 58 32 1140 32 3188 Cuts at: 11460 11492 3496 Size: 32 3504 Fragments arranged by size: 3504 3188 1577 1140 1095 316 230 104 58 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11500 XbaI T'CTAG_A Cuts at: 2684 8384 8836 2684 Size: 5700 452 5348 Fragments arranged by size: 5700 5348 452 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3056 4210 7489 8288 3056 Size: 1154 3279 799 6268 Fragments arranged by size: 6268 3279 1154 799 XhoI C'TCGA_G Cuts at: 1943 3234 6147 6207 1943 Size: 1291 2913 60 7236 Fragments arranged by size: 7236 2913 1291 60 XmaI C'CCGG_G Cuts at: 1870 2024 8825 1870 Size: 154 6801 4545 Fragments arranged by size: 6801 4545 154 XmnI GAAnn'nnTTC Cuts at: 9629 9629 Size: 11500 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9308 276 Size: 53 83 186 936 7774 2468 Fragments arranged by size: 7774 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BsiWI Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI RsrII SacII SfiI SfoI SwaI