Restriction map for
pFN21AA1973
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII Acc65I AclI AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AvrII BamHI BbrPI
BbvCI BcgI BfrI BlnI BsaI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BstAPI Cfr10I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspI
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SpeI SphI Sse8387I
SspBI SwaI XcmI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2863 GTATAC GT^[AC][GT]AC
AccIII 204 TCCGGA T^CCGGA
AflIII 2994 ACGTGT A^C[GA][CT]GT
AhdI 1098 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 157 GTGCAC G^TGCAC
AocI 1112 CCTTAGG CC^T[ACGT]AGG
ApaLI 157 GTGCAC G^TGCAC
AspEI 1098 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 61 TGCGCA TGC^GCA
BclI 659 TGATCA T^GATCA
BglII 2315 AGATCT A^GATCT
BlpI 27 GCTGAGC GC^T[ACGT]AGC
BpmI 3307 CTGGAG CTGGAG(16/14)
Bpu1102I 27 GCTGAGC GC^T[ACGT]AGC
BsaAI 1162 TACGTA [CT]AC^GT[GA]
BsaWI 204 TCCGGA [AT]^CCGG[AT]
BseAI 204 TCCGGA T^CCGGA
BspEI 204 TCCGGA T^CCGGA
BspHI 3060 TCATGA T^CATGA
BsrDI 1122 CATTGC GCAATG(2/0)
BssSI 676 CACGAG CACGAG(-5/-1)
Bst1107I 2863 GTATAC GTA^TAC
BstBI 1015 TTCGAA TT^CGAA
BstEII 1551 GGTTACC G^GT[ACGT]ACC
BstPI 1551 GGTTACC G^GT[ACGT]ACC
BstZ17I 2863 GTATAC GTA^TAC
Bsu36I 1112 CCTTAGG CC^T[ACGT]AGG
CelII 27 GCTGAGC GC^T[ACGT]AGC
DraIII 136 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1098 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1112 CCTTAGG CC^T[ACGT]AGG
EcoNI 2362 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1551 GGTTACC G^GT[ACGT]ACC
EcoT22I 2437 ATGCAT ATGCA^T
FbaI 659 TGATCA T^GATCA
FspI 61 TGCGCA TGC^GCA
MroI 204 TCCGGA T^CCGGA
NsiI 2437 ATGCAT ATGCA^T
NspV 1015 TTCGAA TT^CGAA
PaeR7I 3244 CTCGAG C^TCGAG
PstI 3277 CTGCAG CTGCA^G
RcaI 3060 TCATGA T^CATGA
SapI 2323 GAAGAGC GCTCTTC(1/4)
SfuI 1015 TTCGAA TT^CGAA
SmaI 337 CCCGGG CCC^GGG
SnaBI 1162 TACGTA TAC^GTA
SspI 1292 AATATT AAT^ATT
XbaI 1629 TCTAGA T^CTAGA
XhoI 3244 CTCGAG C^TCGAG
XmaCI 337 CCCGGG C^CCGGG
XmaI 337 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 729 AAATTC [GA]^AATT[CT]
1711 AAATTC
AlwNI 33 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3271 CAGGAGCTG
ApoI 729 AAATTC [GA]^AATT[CT]
1711 AAATTC
AspI 628 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3020 GACCCCGTC
BbeI 267 GGCGCC GGCGC^C
346 GGCGCC
BciVI 2614 GGATAC GTATCC(6/5)
1001 GTATCC
BsaBI 453 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
660 GATCAGCATC
DrdI 252 GACTAGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3333 GACTTGTGAGTC
HgaI 376 GACGC GACGC(5/10)
2518 GACGC
KasI 267 GGCGCC G^GCGCC
346 GGCGCC
MaeII 1163 ACGT A^CGT
2994 ACGT
MamI 453 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
660 GATCAGCATC
NarI 267 GGCGCC GG^CGCC
346 GGCGCC
NcoI 514 CCATGG C^CATGG
1415 CCATGG
PflFI 628 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3020 GACCCCGTC
PflMI 555 CCATACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1077 CCAGAATGTGG
SfoI 267 GGCGCC GGC^GCC
346 GGCGCC
StuI 468 AGGCCT AGG^CCT
1329 AGGCCT
Tth111I 628 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3020 GACCCCGTC
Van91I 555 CCATACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1077 CCAGAATGTGG
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII