(Circular) (Six-base) MAPSORT of: pFN21AA1973.seq Check: 1019 from: 1 to: 8050 pFN21AA1973 8050 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5860 278 Size: 53 83 186 936 4324 2468 Fragments arranged by size: 4324 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4869 5393 1937 Size: 2932 524 4594 Fragments arranged by size: 4594 2932 524 Acc65I G'GTAC_C Cuts at: 5371 5371 Size: 8050 AclI AA'CG_TT Cuts at: 1563 6177 6550 1563 Size: 4614 373 3063 Fragments arranged by size: 4614 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3273 4140 4431 4520 6113 7497 7765 1632 Size: 1641 867 291 89 1593 1384 268 1917 Fragments arranged by size: 1917 1641 1593 1384 867 291 268 89 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7853 AflIII A'CryG_T Cuts at: 1203 4999 6949 7926 1203 Size: 3796 1950 977 1327 Fragments arranged by size: 3796 1950 1327 977 AgeI A'CCGG_T Cuts at: 6881 6881 Size: 8050 AhdI GACnn_n'nnGTC Cuts at: 3108 6779 3108 Size: 3671 4379 AleI CACnn'nnGTG Cuts at: 1166 2978 1166 Size: 1812 6238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3767 4083 1184 Size: 2583 316 5151 Fragments arranged by size: 5151 2583 316 AlwNI CAG_nnn'CTG Cuts at: 2043 5281 5793 7365 2043 Size: 3238 512 1572 2728 Fragments arranged by size: 3238 2728 1572 512 ApaLI G'TGCA_C Cuts at: 2162 6107 7263 2162 Size: 3945 1156 2949 Fragments arranged by size: 3945 2949 1156 ApoI r'AATT_y Cuts at: 1493 2734 3716 5359 5585 5737 7846 1493 Size: 1241 982 1643 226 152 2109 1697 Fragments arranged by size: 2109 1697 1643 1241 982 226 152 AseI AT'TA_AT Cuts at: 160 1032 6604 160 Size: 872 5572 1606 Fragments arranged by size: 5572 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8050 AvaI C'yCGr_G Cuts at: 1870 1943 2342 3981 5249 5375 1870 Size: 73 399 1639 1268 126 4545 Fragments arranged by size: 4545 1639 1268 399 126 73 BamHI G'GATC_C Cuts at: 5380 5747 5380 Size: 367 7683 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2272 2351 5371 6826 Size: 325 217 627 299 186 79 3020 1455 Cuts at: 6826 618 Size: 1842 Fragments arranged by size: 3020 1842 1455 627 325 299 217 186 79 BanII G_rGCy'C Cuts at: 1391 2233 2333 2631 3126 4806 5169 5250 5369 Size: 842 100 298 495 1680 363 81 119 Cuts at: 5369 1391 Size: 4072 Fragments arranged by size: 4072 1680 842 495 363 298 119 100 81 BbeI G_GCGC'C Cuts at: 2276 2355 2276 Size: 79 7971 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2826 3574 3777 4066 4135 928 Size: 971 927 748 203 289 69 4843 Fragments arranged by size: 4843 971 927 748 289 203 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6241 6275 1903 Size: 34 4304 34 3678 Fragments arranged by size: 4304 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3017 4613 5942 7152 3017 Size: 1596 1329 1210 3915 Fragments arranged by size: 3915 1596 1329 1210 BclI T'GATC_A Cuts at: 1548 2664 1548 Size: 1116 6934 BfrBI ATG'CAT Cuts at: 4444 4444 Size: 8050 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2468 3121 3621 Size: 107 122 71 631 605 796 653 500 Cuts at: 3621 3884 6661 136 Size: 263 2777 1525 Fragments arranged by size: 2777 1525 796 653 631 605 500 263 122 107 71 BglII A'GATC_T Cuts at: 4320 8045 4320 Size: 3725 4325 BlpI GC'TnA_GC Cuts at: 2033 5467 2033 Size: 3434 4616 Bme1580I G_kGCm'C Cuts at: 1790 2091 2142 2166 3617 3711 3840 6111 7267 Size: 301 51 24 1451 94 129 2271 1156 Cuts at: 7267 1790 Size: 2573 Fragments arranged by size: 2573 2271 1451 1156 301 129 94 51 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8050 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3028 3855 5781 Size: 416 53 83 399 24 1611 827 1926 Cuts at: 5781 6739 442 Size: 958 1753 Fragments arranged by size: 1926 1753 1611 958 827 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8050 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5333 6710 1239 Size: 4094 1377 2579 Fragments arranged by size: 4094 2579 1377 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2111 2947 2969 3568 6175 7040 7338 2111 Size: 836 22 599 2607 865 298 2823 Fragments arranged by size: 2823 2607 865 836 599 298 22 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6713 882 Size: 617 5214 2219 Fragments arranged by size: 5214 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3169 5841 493 Size: 711 1965 2672 2702 Fragments arranged by size: 2702 2672 1965 711 BsaBI GATnn'nnATC Cuts at: 2462 2669 8044 2462 Size: 207 5375 2468 Fragments arranged by size: 5375 2468 207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2273 2352 2382 4524 Size: 53 83 186 936 740 79 30 2142 Cuts at: 4524 5857 6239 6874 275 Size: 1333 382 635 1451 Fragments arranged by size: 2142 1451 1333 936 740 635 382 186 83 79 53 30 BsaWI w'CCGG_w Cuts at: 2209 6483 6881 7155 7302 7713 2209 Size: 4274 398 274 147 411 2546 Fragments arranged by size: 4274 2546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2002 2032 2354 2384 2522 Size: 30 165 30 599 30 322 30 138 Cuts at: 2522 2552 2894 2924 3979 4009 1178 Size: 30 342 30 1055 30 5219 Fragments arranged by size: 5219 1055 599 342 322 165 138 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2086 2089 2494 2834 5330 1186 Size: 837 63 3 405 340 2496 3906 Fragments arranged by size: 3906 2496 837 405 340 63 3 BseYI C'CCAG_C Cuts at: 2090 3070 3245 4039 5116 7253 7749 2090 Size: 980 175 794 1077 2137 496 2391 Fragments arranged by size: 2391 2137 1077 980 794 496 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2636 3897 4043 1163 Size: 573 900 1261 146 5170 Fragments arranged by size: 5170 1261 900 573 146 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5500 6261 7289 1540 Size: 275 186 3499 761 1028 2301 Fragments arranged by size: 3499 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2166 2306 2631 3165 4863 5169 5250 6111 6196 Size: 140 325 534 1698 306 81 861 85 Cuts at: 6196 7267 2166 Size: 1071 2949 Fragments arranged by size: 2949 1698 1071 861 534 325 306 140 85 81 BsmI GAATG_Cn' Cuts at: 5561 5654 5561 Size: 93 7957 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2142 2166 2233 2306 2333 2631 Size: 399 301 51 24 67 73 27 298 Cuts at: 2631 3126 3165 3617 3711 3840 4806 4863 5169 Size: 495 39 452 94 129 966 57 306 Cuts at: 5169 5250 5369 6111 6196 7267 1391 Size: 81 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 966 742 495 452 399 306 301 298 129 119 94 85 81 73 67 57 51 39 27 24 BspEI T'CCGG_A Cuts at: 2209 7713 2209 Size: 5504 2546 BspHI T'CATG_A Cuts at: 5065 5939 7669 5065 Size: 874 1730 5446 Fragments arranged by size: 5446 1730 874 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5405 844 Size: 4561 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5937 1394 Size: 429 4114 3507 Fragments arranged by size: 4114 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3126 6545 6719 65 Size: 1183 1878 3419 174 1396 Fragments arranged by size: 3419 1878 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6694 6881 1700 Size: 110 4884 187 2869 Fragments arranged by size: 4884 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8050 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8050 BssSI C'ACGA_G Cuts at: 2681 6110 7122 2681 Size: 3429 1012 3609 Fragments arranged by size: 3609 3429 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8050 BstBI TT'CG_AA Cuts at: 3021 6854 3021 Size: 3833 4217 BstEII G'GTnAC_C Cuts at: 1184 3556 1184 Size: 2372 5678 BstXI CCAn_nnnn'nTGG Cuts at: 3961 4026 4221 5002 3961 Size: 65 195 781 7009 Fragments arranged by size: 7009 781 195 65 BstYI r'GATC_y Cuts at: 1972 2121 2940 4320 5127 5380 5747 6132 6149 Size: 149 819 1380 807 253 367 385 17 Cuts at: 6149 7601 7687 7699 8045 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1380 819 807 385 367 346 253 149 86 17 12 BstZ17I GTA'TAC Cuts at: 4870 4870 Size: 8050 Bsu36I CC'TnA_GG Cuts at: 3118 3118 Size: 8050 BtgI C'CryG_G Cuts at: 513 1065 2375 2397 2519 2565 2690 3420 513 Size: 552 1310 22 122 46 125 730 5143 Fragments arranged by size: 5143 1310 730 552 125 122 46 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7311 Fragments arranged by size: 7311 683 56 BtsI GCAGTG_nn' Cuts at: 2533 4065 4411 5566 6360 6380 2533 Size: 1532 346 1155 794 20 4203 Fragments arranged by size: 4203 1532 1155 794 346 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8050 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8015 DraI TTT'AAA Cuts at: 5354 5354 Size: 8050 DraIII CAC_nnn'GTG Cuts at: 2146 2146 Size: 8050 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2263 5344 7057 809 Size: 507 947 3081 1713 1802 Fragments arranged by size: 3081 1802 1713 947 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2196 2314 Size: 54 1425 50 275 18 249 117 118 Cuts at: 2314 2372 2460 3491 3622 4016 4200 5497 6386 Size: 58 88 1031 131 394 184 1297 889 Cuts at: 6386 8015 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1297 1031 889 394 275 249 184 131 118 117 88 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5497 1537 Size: 275 3685 4090 Fragments arranged by size: 4090 3685 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3661 4091 4323 5155 5980 1353 Size: 2308 430 232 832 825 3423 Fragments arranged by size: 3423 2308 832 825 430 232 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2375 3028 6637 7011 7157 387 Size: 1064 138 786 653 3609 374 146 1280 Fragments arranged by size: 3609 1280 1064 786 653 374 146 138 EcoICRI GAG'CTC Cuts at: 2629 5167 5248 2629 Size: 2538 81 5431 Fragments arranged by size: 5431 2538 81 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3273 4140 4431 4520 5333 6113 6710 Size: 393 1641 867 291 89 813 780 597 Cuts at: 6710 7497 7765 1239 Size: 787 268 1524 Fragments arranged by size: 1641 1524 867 813 787 780 597 393 291 268 89 EcoNI CCTnn'n_nnAGG Cuts at: 4371 4371 Size: 8050 EcoO109I rG'GnC_Cy Cuts at: 2365 2920 3978 5024 2365 Size: 555 1058 1046 5391 Fragments arranged by size: 5391 1058 1046 555 EcoRI G'AATT_C Cuts at: 5359 5737 5359 Size: 378 7672 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3562 5355 3562 Size: 1793 6257 FspI TGC'GCA Cuts at: 2068 6556 2068 Size: 4488 3562 HaeII r_GCGC'y Cuts at: 1678 2276 2355 2591 7197 1678 Size: 598 79 236 4606 2531 Fragments arranged by size: 4606 2531 598 236 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 2689 2721 3094 Size: 32 135 32 950 32 135 32 373 Cuts at: 3094 3126 3556 3588 6691 6723 6765 6797 1373 Size: 32 430 32 3103 32 42 32 2626 Fragments arranged by size: 3103 2626 950 430 373 135 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5394 5640 669 Size: 988 281 3456 246 3079 Fragments arranged by size: 3456 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8050 HpaI GTT'AAC Cuts at: 5640 5640 Size: 8050 KasI G'GCGC_C Cuts at: 2272 2351 2272 Size: 79 7971 KpnI G_GTAC'C Cuts at: 5375 5375 Size: 8050 MfeI C'AATT_G Cuts at: 5649 5649 Size: 8050 MluI A'CGCG_T Cuts at: 7926 7926 Size: 8050 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3247 3550 4202 5315 7164 7348 1305 Size: 1942 303 652 1113 1849 184 2007 Fragments arranged by size: 2007 1942 1849 1113 652 303 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2374 2462 3493 3624 4018 Size: 54 1425 343 542 88 1031 131 394 Cuts at: 4018 4202 10 Size: 184 3858 Fragments arranged by size: 3858 1425 1031 542 394 343 184 131 88 54 MslI CAynn'nnrTG Cuts at: 518 1166 2660 2978 3266 3971 6008 6367 6526 Size: 648 1494 318 288 705 2037 359 159 Cuts at: 6526 518 Size: 2042 Fragments arranged by size: 2042 2037 1494 705 648 359 318 288 159 MspA1I CmG'CkG Cuts at: 1606 3453 5116 5287 5466 6143 7291 7536 1606 Size: 1847 1663 171 179 677 1148 245 2120 Fragments arranged by size: 2120 1847 1663 1148 677 245 179 171 NarI GG'CG_CC Cuts at: 2273 2352 2273 Size: 79 7971 NcoI C'CATG_G Cuts at: 513 1065 2519 3420 513 Size: 552 1454 901 5143 Fragments arranged by size: 5143 1454 901 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8050 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8050 NotI GC'GGCC_GC Cuts at: 5497 5497 Size: 8050 NsiI A_TGCA'T Cuts at: 4446 4446 Size: 8050 NspI r_CATG'y Cuts at: 1132 1229 5408 6953 1132 Size: 97 4179 1545 2229 Fragments arranged by size: 4179 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8050 PciI A'CATG_T Cuts at: 6949 6949 Size: 8050 PflMI CCAn_nnn'nTGG Cuts at: 1108 2566 3088 1108 Size: 1458 522 6070 Fragments arranged by size: 6070 1458 522 PmeI GTTT'AAAC Cuts at: 5354 5354 Size: 8050 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3222 3254 6119 6151 7632 7664 3222 Size: 32 2865 32 1481 32 3608 Fragments arranged by size: 3608 2865 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2920 3978 5024 2920 Size: 1058 1046 5946 Fragments arranged by size: 5946 1058 1046 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8050 PsiI TTA'TAA Cuts at: 5620 5620 Size: 8050 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2774 2806 2774 Size: 32 8018 PstI C_TGCA'G Cuts at: 830 5286 5402 830 Size: 4456 116 3478 Fragments arranged by size: 4456 3478 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8050 PvuII CAG'CTG Cuts at: 3453 5116 5287 3453 Size: 1663 171 6216 Fragments arranged by size: 6216 1663 171 SacI G_AGCT'C Cuts at: 2631 5169 5250 2631 Size: 2538 81 5431 Fragments arranged by size: 5431 2538 81 SalI G'TCGA_C Cuts at: 1936 5392 1936 Size: 3456 4594 SanDI GG'GwC_CC Cuts at: 5024 5024 Size: 8050 SapI GCTCTTCn'nnn_ Cuts at: 4323 4323 Size: 8050 SbfI CC_TGCA'GG Cuts at: 5402 5402 Size: 8050 ScaI AGT'ACT Cuts at: 6298 8010 6298 Size: 1712 6338 SfcI C'TryA_G Cuts at: 826 1045 2936 3201 5282 5398 6533 7214 7405 Size: 219 1891 265 2081 116 1135 681 191 Cuts at: 7405 826 Size: 1471 Fragments arranged by size: 2081 1891 1471 1135 681 265 219 191 116 SfoI GGC'GCC Cuts at: 2274 2353 2274 Size: 79 7971 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8050 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8050 SmaI CCC'GGG Cuts at: 1872 2344 5377 1872 Size: 472 3033 4545 Fragments arranged by size: 4545 3033 472 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 2926 2948 3547 5249 6154 Size: 197 926 183 800 22 599 1702 905 Cuts at: 6154 7055 7317 820 Size: 901 262 1553 Fragments arranged by size: 1702 1553 926 905 901 800 599 262 197 183 22 SnaBI TAC'GTA Cuts at: 493 3169 5841 493 Size: 2676 2672 2702 Fragments arranged by size: 2702 2676 2672 SpeI A'CTAG_T Cuts at: 152 152 Size: 8050 SphI G_CATG'C Cuts at: 1132 5408 1132 Size: 4276 3774 SspI AAT'ATT Cuts at: 5 52 3299 5974 5 Size: 47 3247 2675 2081 Fragments arranged by size: 3247 2675 2081 47 StuI AGG'CCT Cuts at: 1517 2475 3336 1517 Size: 958 861 6231 Fragments arranged by size: 6231 958 861 StyI C'CwwG_G Cuts at: 513 1065 2519 2875 3420 4119 4372 5153 5489 Size: 552 1454 356 545 699 253 781 336 Cuts at: 5489 513 Size: 3074 Fragments arranged by size: 3074 1454 781 699 552 545 356 336 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2930 2986 3055 3845 4654 6077 6094 Size: 1255 1350 56 69 790 809 1423 17 Cuts at: 6094 6247 6432 6877 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1423 1350 1255 809 790 445 185 153 69 56 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6296 8008 96 Size: 274 80 33 51 1443 4319 1712 138 Fragments arranged by size: 4319 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2354 2386 8010 8042 2354 Size: 32 5624 32 2362 Fragments arranged by size: 5624 2362 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2636 5028 1316 Size: 1320 2392 4338 Fragments arranged by size: 4338 2392 1320 XbaI T'CTAG_A Cuts at: 3634 5386 3634 Size: 1752 6298 XhoI C'TCGA_G Cuts at: 1943 5249 1943 Size: 3306 4744 XmaI C'CCGG_G Cuts at: 1870 2342 5375 1870 Size: 472 3033 4545 Fragments arranged by size: 4545 3033 472 XmnI GAAnn'nnTTC Cuts at: 6179 6179 Size: 8050 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5858 276 Size: 53 83 186 936 4324 2468 Fragments arranged by size: 4324 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SexAI SfiI SrfI SwaI XcmI