(Circular) (Six-base) MAPSORT of: pFN21AA1938.seq Check: 6668 from: 1 to: 8734 pFN21AA1938 8734 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6544 278 Size: 53 83 186 936 5008 2468 Fragments arranged by size: 5008 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2784 3423 3582 6077 1937 Size: 847 639 159 2495 4594 Fragments arranged by size: 4594 2495 847 639 159 Acc65I G'GTAC_C Cuts at: 3090 6055 3090 Size: 2965 5769 AclI AA'CG_TT Cuts at: 1563 6861 7234 1563 Size: 5298 373 3063 Fragments arranged by size: 5298 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2050 3719 4151 4295 5411 6797 8181 8449 Size: 418 1669 432 144 1116 1386 1384 268 Cuts at: 8449 1632 Size: 1917 Fragments arranged by size: 1917 1669 1386 1384 1116 432 418 268 144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8537 AflIII A'CryG_T Cuts at: 1203 5634 7633 8610 1203 Size: 4431 1999 977 1327 Fragments arranged by size: 4431 1999 1327 977 AgeI A'CCGG_T Cuts at: 3426 7565 3426 Size: 4139 4595 AhdI GACnn_n'nnGTC Cuts at: 2258 4312 7463 2258 Size: 2054 3151 3529 Fragments arranged by size: 3529 3151 2054 AleI CACnn'nnGTG Cuts at: 1166 4905 1166 Size: 3739 4995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2261 5819 1184 Size: 1077 3558 4099 Fragments arranged by size: 4099 3558 1077 AlwNI CAG_nnn'CTG Cuts at: 2482 3901 4765 5150 5456 6477 8049 2482 Size: 1419 864 385 306 1021 1572 3167 Fragments arranged by size: 3167 1572 1419 1021 864 385 306 ApaI G_GGCC'C Cuts at: 2518 2518 Size: 8734 ApaLI G'TGCA_C Cuts at: 2630 3351 3595 6791 7947 2630 Size: 721 244 3196 1156 3417 Fragments arranged by size: 3417 3196 1156 721 244 ApoI r'AATT_y Cuts at: 1493 3539 6043 6269 6421 8530 1493 Size: 2046 2504 226 152 2109 1697 Fragments arranged by size: 2504 2109 2046 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7288 160 Size: 872 6256 1606 Fragments arranged by size: 6256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8734 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2158 2317 2817 3157 3688 4326 Size: 73 73 142 159 500 340 531 638 Cuts at: 4326 6059 1870 Size: 1733 4545 Fragments arranged by size: 4545 1733 638 531 500 340 159 142 73 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2833 2866 2833 Size: 33 8701 BamHI G'GATC_C Cuts at: 6064 6431 6064 Size: 367 8367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 3069 3090 6055 7510 Size: 325 217 627 576 706 21 2965 1455 Cuts at: 7510 618 Size: 1842 Fragments arranged by size: 2965 1842 1455 706 627 576 325 217 21 BanII G_rGCy'C Cuts at: 1391 2518 2598 2856 3158 3315 4071 4917 6053 Size: 1127 80 258 302 157 756 846 1136 Cuts at: 6053 1391 Size: 4072 Fragments arranged by size: 4072 1136 1127 846 756 302 258 157 80 BbeI G_GCGC'C Cuts at: 3073 3073 Size: 8734 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2914 928 Size: 971 1015 6748 Fragments arranged by size: 6748 1015 971 BbvCI CC'TCA_GC Cuts at: 2583 4233 4721 5330 2583 Size: 1650 488 609 5987 Fragments arranged by size: 5987 1650 609 488 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6925 6959 1903 Size: 34 4988 34 3678 Fragments arranged by size: 4988 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6626 7836 6626 Size: 1210 7524 BclI T'GATC_A Cuts at: 1548 1548 Size: 8734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2383 3062 7345 Size: 107 122 71 631 605 711 679 4283 Cuts at: 7345 136 Size: 1525 Fragments arranged by size: 4283 1525 711 679 631 605 122 107 71 BglII A'GATC_T Cuts at: 4002 8729 4002 Size: 4727 4007 BlpI GC'TnA_GC Cuts at: 4112 5847 6151 4112 Size: 1735 304 6695 Fragments arranged by size: 6695 1735 304 Bme1580I G_kGCm'C Cuts at: 1790 2368 2518 2634 2971 3355 3599 5518 6795 Size: 578 150 116 337 384 244 1919 1277 Cuts at: 6795 7951 1790 Size: 1156 2573 Fragments arranged by size: 2573 1919 1277 1156 578 384 337 244 150 116 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3813 6465 7423 Size: 416 53 83 399 24 2396 2652 958 Cuts at: 7423 442 Size: 1753 Fragments arranged by size: 2652 2396 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8734 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 2563 4984 5629 2031 Size: 532 2421 645 5136 Fragments arranged by size: 5136 2421 645 532 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3983 5060 5172 5747 7394 1239 Size: 2744 1077 112 575 1647 2579 Fragments arranged by size: 2744 2579 1647 1077 575 112 Bpu10I CC'TnA_GC Cuts at: 2402 2583 3169 4233 4721 5330 5747 5886 2402 Size: 181 586 1064 488 609 417 139 5250 Fragments arranged by size: 5250 1064 609 586 488 417 181 139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3506 4603 5528 6859 7724 8022 3506 Size: 1097 925 1331 865 298 4218 Fragments arranged by size: 4218 1331 1097 925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2019 5482 7397 882 Size: 617 520 3463 1915 2219 Fragments arranged by size: 3463 2219 1915 617 520 BsaAI yAC'GTr Cuts at: 493 1204 6525 493 Size: 711 5321 2702 Fragments arranged by size: 5321 2702 711 BsaBI GATnn'nnATC Cuts at: 8728 8728 Size: 8734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3070 4672 6541 6923 Size: 53 83 186 936 1537 1602 1869 382 Cuts at: 6923 7558 275 Size: 635 1451 Fragments arranged by size: 1869 1602 1537 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2217 2457 3426 5643 7167 7565 7839 7986 8397 Size: 240 969 2217 1524 398 274 147 411 Cuts at: 8397 2217 Size: 2554 Fragments arranged by size: 2554 2217 1524 969 411 398 274 240 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2314 2344 1178 Size: 30 165 30 911 30 7568 Fragments arranged by size: 7568 911 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2236 2335 3353 4145 5615 5618 5828 5831 Size: 1050 99 1018 792 1470 3 210 3 Cuts at: 5831 1186 Size: 4089 Fragments arranged by size: 4089 1470 1050 1018 792 210 99 3 3 BseYI C'CCAG_C Cuts at: 3065 3802 4107 4135 4758 5965 7937 8433 3065 Size: 737 305 28 623 1207 1972 496 3366 Fragments arranged by size: 3366 1972 1207 737 623 496 305 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2598 3321 4450 4598 5615 1163 Size: 573 862 723 1129 148 1017 4282 Fragments arranged by size: 4282 1129 1017 862 723 573 148 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2715 4599 6184 6945 7973 1540 Size: 275 186 714 1884 1585 761 1028 2301 Fragments arranged by size: 2301 1884 1585 1028 761 714 275 186 BsiHKAI G_wGCw'C Cuts at: 2598 2634 2856 2937 3355 3447 3553 3599 3621 Size: 36 222 81 418 92 106 46 22 Cuts at: 3621 4071 6795 6880 7951 2598 Size: 450 2724 85 1071 3381 Fragments arranged by size: 3381 2724 1071 450 418 222 106 92 85 81 46 36 22 BsmI GAATG_Cn' Cuts at: 5413 6245 6338 5413 Size: 832 93 7809 Fragments arranged by size: 7809 832 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2245 2245 Size: 8734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2368 2518 2598 2634 2856 2937 2971 Size: 399 578 150 80 36 222 81 34 Cuts at: 2971 3158 3315 3355 3447 3553 3599 3621 4071 Size: 187 157 40 92 106 46 22 450 Cuts at: 4071 4917 5518 6053 6795 6880 7951 1391 Size: 846 601 535 742 85 1071 2174 Fragments arranged by size: 2174 1071 846 742 601 578 535 450 399 222 187 157 150 106 92 85 81 80 46 40 36 34 22 BspEI T'CCGG_A Cuts at: 8397 8397 Size: 8734 BspHI T'CATG_A Cuts at: 2551 3948 6623 8353 2551 Size: 1397 2675 1730 2932 Fragments arranged by size: 2932 2675 1730 1397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2001 2961 3639 4617 5094 6089 844 Size: 1157 960 678 978 477 995 3489 Fragments arranged by size: 3489 1157 995 978 960 678 477 BsrBI CCG'CTC Cuts at: 1394 1823 2901 4222 5163 5461 5527 5626 5665 Size: 429 1078 1321 941 298 66 99 39 Cuts at: 5665 6621 1394 Size: 956 3507 Fragments arranged by size: 3507 1321 1078 956 941 429 298 99 66 39 BsrDI GCAATG_nn' Cuts at: 65 1248 3879 5204 7229 7403 65 Size: 1183 2631 1325 2025 174 1396 Fragments arranged by size: 2631 2025 1396 1325 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2269 2956 3426 5574 7378 7565 1700 Size: 110 459 687 470 2148 1804 187 2869 Fragments arranged by size: 2869 2148 1804 687 470 459 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8734 BssSI C'ACGA_G Cuts at: 4468 6794 7806 4468 Size: 2326 1012 5396 Fragments arranged by size: 5396 2326 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4504 5095 794 Size: 3710 591 4433 Fragments arranged by size: 4433 3710 591 BstBI TT'CG_AA Cuts at: 4586 7538 4586 Size: 2952 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8734 BstXI CCAn_nnnn'nTGG Cuts at: 4688 4907 4688 Size: 219 8515 BstYI r'GATC_y Cuts at: 1972 2478 3122 4002 6064 6431 6816 6833 8285 Size: 506 644 880 2062 367 385 17 1452 Cuts at: 8285 8371 8383 8729 1972 Size: 86 12 346 1977 Fragments arranged by size: 2062 1977 1452 880 644 506 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5143 5143 Size: 8734 BtgI C'CryG_G Cuts at: 513 1065 2287 3243 4837 4855 4942 5315 5841 Size: 552 1222 956 1594 18 87 373 526 Cuts at: 5841 5945 513 Size: 104 3302 Fragments arranged by size: 3302 1594 1222 956 552 526 373 104 87 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2063 3739 487 Size: 683 56 837 1676 5482 Fragments arranged by size: 5482 1676 837 683 56 BtsI GCAGTG_nn' Cuts at: 2581 3092 3121 3676 4076 4142 4585 6250 7044 Size: 511 29 555 400 66 443 1665 794 Cuts at: 7044 7064 2581 Size: 20 4251 Fragments arranged by size: 4251 1665 794 555 511 443 400 66 29 20 ClaI AT'CG_AT Cuts at: 1554 5561 1554 Size: 4007 4727 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8699 DraI TTT'AAA Cuts at: 6038 6038 Size: 8734 DraIII CAC_nnn'GTG Cuts at: 3682 3682 Size: 8734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3507 7741 809 Size: 507 2191 4234 1802 Fragments arranged by size: 4234 2191 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2712 3054 3408 Size: 54 1425 50 275 18 882 342 354 Cuts at: 3408 3903 4561 4596 4834 4852 5258 6181 7070 Size: 495 658 35 238 18 406 923 889 Cuts at: 7070 8699 8 Size: 1629 43 Fragments arranged by size: 1629 1425 923 889 882 658 495 406 354 342 275 238 54 50 43 35 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2712 4596 6181 1537 Size: 275 900 1884 1585 4090 Fragments arranged by size: 4090 1884 1585 900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 3483 3788 4802 4975 5526 5649 5735 Size: 968 1162 305 1014 173 551 123 86 Cuts at: 5735 5864 6664 1353 Size: 129 800 3423 Fragments arranged by size: 3423 1162 1014 968 800 551 305 173 129 123 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5388 7321 7695 7841 387 Size: 1064 138 3799 1933 374 146 1280 Fragments arranged by size: 3799 1933 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2596 2854 4069 2596 Size: 258 1215 7261 Fragments arranged by size: 7261 1215 258 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 3719 3983 4151 4295 5060 5172 Size: 393 418 1669 264 168 144 765 112 Cuts at: 5172 5411 5747 6797 7394 8181 8449 1239 Size: 239 336 1050 597 787 268 1524 Fragments arranged by size: 1669 1524 1050 787 765 597 418 393 336 268 264 239 168 144 112 EcoNI CCTnn'n_nnAGG Cuts at: 2648 3659 4679 5333 2648 Size: 1011 1020 654 6049 Fragments arranged by size: 6049 1020 1011 654 EcoO109I rG'GnC_Cy Cuts at: 2081 4288 4606 4823 4963 4978 5147 2081 Size: 2207 318 217 140 15 169 5668 Fragments arranged by size: 5668 2207 318 217 169 140 15 EcoRI G'AATT_C Cuts at: 3539 6043 6421 3539 Size: 2504 378 5852 Fragments arranged by size: 5852 2504 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3952 3952 Size: 8734 FspI TGC'GCA Cuts at: 3733 7240 3733 Size: 3507 5227 HaeII r_GCGC'y Cuts at: 1678 3073 3777 7881 1678 Size: 1395 704 4104 2531 Fragments arranged by size: 4104 2531 1395 704 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2010 2042 2244 2276 2470 Size: 32 135 32 438 32 202 32 194 Cuts at: 2470 2502 2722 2754 3528 3560 3994 4026 4298 Size: 32 220 32 774 32 434 32 272 Cuts at: 4298 4330 5698 5730 7375 7407 7449 7481 1373 Size: 32 1368 32 1645 32 42 32 2626 Fragments arranged by size: 2626 1645 1368 774 438 434 272 220 202 194 135 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3670 4516 5232 6078 6324 669 Size: 988 281 1732 846 716 846 246 3079 Fragments arranged by size: 3079 1732 988 846 846 716 281 246 HindIII A'AGCT_T Cuts at: 748 2530 748 Size: 1782 6952 HpaI GTT'AAC Cuts at: 6324 6324 Size: 8734 KasI G'GCGC_C Cuts at: 3069 3069 Size: 8734 KpnI G_GTAC'C Cuts at: 3094 6059 3094 Size: 2965 5769 MfeI C'AATT_G Cuts at: 6333 6333 Size: 8734 MluI A'CGCG_T Cuts at: 8610 8610 Size: 8734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2114 5505 5664 5939 7848 8032 1305 Size: 809 3391 159 275 1909 184 2007 Fragments arranged by size: 3391 2007 1909 809 275 184 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 3056 3410 3905 4854 10 Size: 54 1425 343 1224 354 495 949 3890 Fragments arranged by size: 3890 1425 1224 949 495 354 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2550 3410 4905 6692 7051 7210 518 Size: 648 1384 860 1495 1787 359 159 2042 Fragments arranged by size: 2042 1787 1495 1384 860 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2170 2582 2746 5175 5268 5358 5367 5854 Size: 564 412 164 2429 93 90 9 487 Cuts at: 5854 6150 6827 7975 8220 1606 Size: 296 677 1148 245 2120 Fragments arranged by size: 2429 2120 1148 677 564 487 412 296 245 164 93 90 9 NaeI GCC'GGC Cuts at: 2958 5576 2958 Size: 2618 6116 NarI GG'CG_CC Cuts at: 3070 3070 Size: 8734 NcoI C'CATG_G Cuts at: 513 1065 3243 4837 4855 4942 5841 513 Size: 552 2178 1594 18 87 899 3406 Fragments arranged by size: 3406 2178 1594 899 552 87 18 NdeI CA'TA_TG Cuts at: 387 387 Size: 8734 NgoMIV G'CCGG_C Cuts at: 2956 5574 2956 Size: 2618 6116 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8734 NotI GC'GGCC_GC Cuts at: 6181 6181 Size: 8734 NspI r_CATG'y Cuts at: 1132 1229 2872 4085 4717 5098 5488 5638 6092 Size: 97 1643 1213 632 381 390 150 454 Cuts at: 6092 7637 1132 Size: 1545 2229 Fragments arranged by size: 2229 1643 1545 1213 632 454 390 381 150 97 PasI CC'CwG_GG Cuts at: 1348 2336 4681 4919 1348 Size: 988 2345 238 5163 Fragments arranged by size: 5163 2345 988 238 PciI A'CATG_T Cuts at: 5634 7633 5634 Size: 1999 6735 PflMI CCAn_nnn'nTGG Cuts at: 1108 3901 4400 4943 5300 1108 Size: 2793 499 543 357 4542 Fragments arranged by size: 4542 2793 543 499 357 PfoI T'CCnGG_A Cuts at: 2211 5076 2211 Size: 2865 5869 PmeI GTTT'AAAC Cuts at: 6038 6038 Size: 8734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6803 6835 8316 8348 6803 Size: 32 1481 32 7189 Fragments arranged by size: 7189 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2081 4288 4606 4823 4963 4978 2081 Size: 2207 318 217 140 15 5837 Fragments arranged by size: 5837 2207 318 217 140 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8734 PsiI TTA'TAA Cuts at: 6304 6304 Size: 8734 PspOMI G'GGCC_C Cuts at: 2514 2514 Size: 8734 PspXI vC'TCGA_Gb Cuts at: 2317 4326 2317 Size: 2009 6725 PstI C_TGCA'G Cuts at: 830 2745 3372 5091 5994 6086 830 Size: 1915 627 1719 903 92 3478 Fragments arranged by size: 3478 1915 1719 903 627 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8734 PvuII CAG'CTG Cuts at: 2170 2582 2170 Size: 412 8322 SacI G_AGCT'C Cuts at: 2598 2856 4071 2598 Size: 258 1215 7261 Fragments arranged by size: 7261 1215 258 SalI G'TCGA_C Cuts at: 1936 6076 1936 Size: 4140 4594 SanDI GG'GwC_CC Cuts at: 2081 4978 2081 Size: 2897 5837 SbfI CC_TGCA'GG Cuts at: 6086 6086 Size: 8734 ScaI AGT'ACT Cuts at: 5552 6982 8694 5552 Size: 1430 1712 5592 Fragments arranged by size: 5592 1712 1430 SfcI C'TryA_G Cuts at: 826 1045 2741 3368 4948 5020 5087 5990 6082 Size: 219 1696 627 1580 72 67 903 92 Cuts at: 6082 7217 7898 8089 826 Size: 1135 681 191 1471 Fragments arranged by size: 1696 1580 1471 1135 903 681 627 219 191 92 72 67 SfoI GGC'GCC Cuts at: 3071 3071 Size: 8734 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8734 SmaI CCC'GGG Cuts at: 1872 2819 6061 1872 Size: 947 3242 4545 Fragments arranged by size: 4545 3242 947 SmlI C'TyrA_G Cuts at: 820 1017 1943 2016 2317 3521 4326 4618 5543 Size: 197 926 73 301 1204 805 292 925 Cuts at: 5543 6838 7739 8001 820 Size: 1295 901 262 1553 Fragments arranged by size: 1553 1295 1204 926 925 901 805 301 292 262 197 73 SnaBI TAC'GTA Cuts at: 493 6525 493 Size: 6032 2702 SpeI A'CTAG_T Cuts at: 152 3355 152 Size: 3203 5531 SphI G_CATG'C Cuts at: 1132 4085 4717 6092 1132 Size: 2953 632 1375 3774 Fragments arranged by size: 3774 2953 1375 632 SspI AAT'ATT Cuts at: 5 52 6658 5 Size: 47 6606 2081 Fragments arranged by size: 6606 2081 47 StuI AGG'CCT Cuts at: 1517 4332 4628 1517 Size: 2815 296 5623 Fragments arranged by size: 5623 2815 296 StyI C'CwwG_G Cuts at: 513 1065 3243 3387 3885 4374 4644 4837 4855 Size: 552 2178 144 498 489 270 193 18 Cuts at: 4855 4942 5841 5907 6173 513 Size: 87 899 66 266 3074 Fragments arranged by size: 3074 2178 899 552 498 489 270 266 193 144 87 66 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2694 4721 6761 6778 6931 7116 7561 Size: 1255 1114 2027 2040 17 153 185 445 Cuts at: 7561 325 Size: 1498 Fragments arranged by size: 2040 2027 1498 1255 1114 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2349 3374 5535 Size: 274 80 33 51 1443 372 1025 2161 Cuts at: 5535 5550 6980 8692 96 Size: 15 1430 1712 138 Fragments arranged by size: 2161 1712 1443 1430 1025 372 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2314 2346 3283 3315 5084 5107 5116 5139 8694 Size: 32 937 32 1769 23 9 23 3555 Cuts at: 8694 8726 2314 Size: 32 2322 Fragments arranged by size: 3555 2322 1769 937 32 32 32 23 23 9 Tth111I GACn'n_nGTC Cuts at: 1316 2918 4748 1316 Size: 1602 1830 5302 Fragments arranged by size: 5302 1830 1602 XbaI T'CTAG_A Cuts at: 6070 6070 Size: 8734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4904 5117 5647 4904 Size: 213 530 7991 Fragments arranged by size: 7991 530 213 XhoI C'TCGA_G Cuts at: 1943 2016 2317 4326 1943 Size: 73 301 2009 6351 Fragments arranged by size: 6351 2009 301 73 XmaI C'CCGG_G Cuts at: 1870 2817 6059 1870 Size: 947 3242 4545 Fragments arranged by size: 4545 3242 947 XmnI GAAnn'nnTTC Cuts at: 6863 6863 Size: 8734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6542 276 Size: 53 83 186 936 5008 2468 Fragments arranged by size: 5008 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI