(Circular) (Six-base) MAPSORT of: pFN21AA1825.seq Check: 1057 from: 1 to: 8317 pFN21AA1825 8317 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2399 4467 4498 2399 Size: 2068 31 6218 Fragments arranged by size: 6218 2068 31 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6127 278 Size: 53 83 186 936 4591 2468 Fragments arranged by size: 4591 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2913 3045 5214 5313 5660 1937 Size: 976 132 2169 99 347 4594 Fragments arranged by size: 4594 2169 976 347 132 99 Acc65I G'GTAC_C Cuts at: 3401 5638 3401 Size: 2237 6080 AclI AA'CG_TT Cuts at: 1563 6444 6817 1563 Size: 4881 373 3063 Fragments arranged by size: 4881 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3233 3914 4580 4640 6380 7764 8032 1632 Size: 1601 681 666 60 1740 1384 268 1917 Fragments arranged by size: 1917 1740 1601 1384 681 666 268 60 AfeI AGC'GCT Cuts at: 3872 3872 Size: 8317 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8120 AflIII A'CryG_T Cuts at: 1203 2865 3540 7216 8193 1203 Size: 1662 675 3676 977 1327 Fragments arranged by size: 3676 1662 1327 977 675 AgeI A'CCGG_T Cuts at: 7148 7148 Size: 8317 AhdI GACnn_n'nnGTC Cuts at: 7046 7046 Size: 8317 AleI CACnn'nnGTG Cuts at: 1166 3170 3758 4946 1166 Size: 2004 588 1188 4537 Fragments arranged by size: 4537 2004 1188 588 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2051 2300 3299 4394 4397 1184 Size: 867 249 999 1095 3 5104 Fragments arranged by size: 5104 1095 999 867 249 3 AlwNI CAG_nnn'CTG Cuts at: 2255 3856 4285 4483 4816 5068 5533 6060 7632 Size: 1601 429 198 333 252 465 527 1572 Cuts at: 7632 2255 Size: 2940 Fragments arranged by size: 2940 1601 1572 527 465 429 333 252 198 ApaI G_GGCC'C Cuts at: 2050 3715 3973 2050 Size: 1665 258 6394 Fragments arranged by size: 6394 1665 258 ApaLI G'TGCA_C Cuts at: 2369 3445 5195 6374 7530 2369 Size: 1076 1750 1179 1156 3156 Fragments arranged by size: 3156 1750 1179 1156 1076 ApoI r'AATT_y Cuts at: 1493 2522 2666 3861 5626 5852 6004 8113 1493 Size: 1029 144 1195 1765 226 152 2109 1697 Fragments arranged by size: 2109 1765 1697 1195 1029 226 152 144 AseI AT'TA_AT Cuts at: 160 1032 6871 160 Size: 872 5839 1606 Fragments arranged by size: 5839 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8317 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2353 4041 4107 4392 4692 4826 Size: 73 144 266 1688 66 285 300 134 Cuts at: 4826 5127 5246 5390 5642 1870 Size: 301 119 144 252 4545 Fragments arranged by size: 4545 1688 301 300 285 266 252 144 144 134 119 73 66 BamHI G'GATC_C Cuts at: 3564 5647 6014 3564 Size: 2083 367 5867 Fragments arranged by size: 5867 2083 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2193 2243 2437 2695 Size: 325 217 627 248 158 50 194 258 Cuts at: 2695 2775 3004 3164 3401 3752 4598 4613 5638 Size: 80 229 160 237 351 846 15 1025 Cuts at: 5638 7093 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1025 846 627 351 325 258 248 237 229 217 194 160 158 80 50 15 BanII G_rGCy'C Cuts at: 1391 2050 2469 2772 3093 3558 3715 3935 3973 Size: 659 419 303 321 465 157 220 38 Cuts at: 3973 4054 4400 4764 5246 5636 1391 Size: 81 346 364 482 390 4072 Fragments arranged by size: 4072 659 482 465 419 390 364 346 321 303 220 157 81 38 BbeI G_GCGC'C Cuts at: 2197 4617 2197 Size: 2420 5897 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2502 2643 3105 928 Size: 971 603 141 462 6140 Fragments arranged by size: 6140 971 603 462 141 BbvCI CC'TCA_GC Cuts at: 3104 3104 Size: 8317 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3018 3052 6508 6542 1903 Size: 34 1081 34 3456 34 3678 Fragments arranged by size: 3678 3456 1081 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6209 7419 6209 Size: 1210 7107 BclI T'GATC_A Cuts at: 1548 4239 4923 1548 Size: 2691 684 4942 Fragments arranged by size: 4942 2691 684 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2074 2123 Size: 107 122 71 631 605 337 65 49 Cuts at: 2123 2173 4440 4523 5484 6928 136 Size: 50 2267 83 961 1444 1525 Fragments arranged by size: 2267 1525 1444 961 631 605 337 122 107 83 71 65 50 49 BglII A'GATC_T Cuts at: 4035 8312 4035 Size: 4277 4040 BlpI GC'TnA_GC Cuts at: 5734 5734 Size: 8317 Bme1580I G_kGCm'C Cuts at: 1790 2040 2050 2373 3167 3449 3715 3892 3973 Size: 250 10 323 794 282 266 177 81 Cuts at: 3973 4007 5199 6378 7534 1790 Size: 34 1192 1179 1156 2573 Fragments arranged by size: 2573 1192 1179 1156 794 323 282 266 250 177 81 34 10 BmgBI CAC'GTC Cuts at: 1318 3016 3151 4609 4633 1318 Size: 1698 135 1458 24 5002 Fragments arranged by size: 5002 1698 1458 135 24 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3674 3994 4865 Size: 416 53 83 399 24 2257 320 871 Cuts at: 4865 6048 7006 442 Size: 1183 958 1753 Fragments arranged by size: 2257 1753 1183 958 871 416 399 320 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8317 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4375 4375 Size: 8317 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2576 3113 3350 3461 4151 4439 4720 5045 Size: 1337 537 237 111 690 288 281 325 Cuts at: 5045 5168 6977 1239 Size: 123 1809 2579 Fragments arranged by size: 2579 1809 1337 690 537 325 288 281 237 123 111 Bpu10I CC'TnA_GC Cuts at: 3104 3477 4662 4709 4805 5204 3104 Size: 373 1185 47 96 399 6217 Fragments arranged by size: 6217 1185 399 373 96 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 4127 4163 5099 6442 7307 7605 2525 Size: 1602 36 936 1343 865 298 3237 Fragments arranged by size: 3237 1602 1343 936 865 298 36 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3326 4171 4815 6980 882 Size: 617 1827 845 644 2165 2219 Fragments arranged by size: 2219 2165 1827 845 644 617 BsaAI yAC'GTr Cuts at: 493 1204 4273 4576 4921 6108 493 Size: 711 3069 303 345 1187 2702 Fragments arranged by size: 3069 2702 1187 711 345 303 BsaBI GATnn'nnATC Cuts at: 8311 8311 Size: 8317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2194 4614 6124 6506 Size: 53 83 186 936 661 2420 1510 382 Cuts at: 6506 7141 275 Size: 635 1451 Fragments arranged by size: 2420 1510 1451 936 661 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2877 3254 3561 6750 7148 7422 7569 7980 2877 Size: 377 307 3189 398 274 147 411 3214 Fragments arranged by size: 3214 3189 411 398 377 307 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 2957 2987 4041 Size: 30 165 30 1038 30 486 30 1054 Cuts at: 4041 4071 1178 Size: 30 5424 Fragments arranged by size: 5424 1054 1038 486 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4790 5441 5449 1186 Size: 3604 651 8 4054 Fragments arranged by size: 4054 3604 651 8 BseYI C'CCAG_C Cuts at: 2262 2268 2411 3413 3928 4066 4081 4476 4567 Size: 6 143 1002 515 138 15 395 91 Cuts at: 4567 7520 8016 2262 Size: 2953 496 2563 Fragments arranged by size: 2953 2563 1002 515 496 395 143 138 91 15 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 2377 2462 2867 3131 3197 3532 Size: 573 570 71 85 405 264 66 335 Cuts at: 3532 3968 4156 4271 4526 4924 1163 Size: 436 188 115 255 398 4556 Fragments arranged by size: 4556 573 570 436 405 398 335 264 255 188 115 85 71 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2983 3468 3795 3960 4757 5569 Size: 275 186 982 485 327 165 797 812 Cuts at: 5569 5767 6528 7556 1540 Size: 198 761 1028 2301 Fragments arranged by size: 2301 1028 982 812 797 761 485 327 275 198 186 165 BsiHKAI G_wGCw'C Cuts at: 2373 2469 3449 5199 6378 6463 7534 2373 Size: 96 980 1750 1179 85 1071 3156 Fragments arranged by size: 3156 1750 1179 1071 980 96 85 BsmI GAATG_Cn' Cuts at: 4222 5828 5921 4222 Size: 1606 93 6618 Fragments arranged by size: 6618 1606 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2977 3654 2977 Size: 677 7640 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2050 2373 2469 2772 3093 3167 Size: 399 250 10 323 96 303 321 74 Cuts at: 3167 3449 3558 3715 3892 3935 3973 4007 4054 Size: 282 109 157 177 43 38 34 47 Cuts at: 4054 4400 4764 5199 5246 5636 6378 6463 7534 Size: 346 364 435 47 390 742 85 1071 Cuts at: 7534 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 435 399 390 364 346 323 321 303 282 250 177 157 109 96 85 74 47 47 43 38 34 10 BspEI T'CCGG_A Cuts at: 2877 3561 7980 2877 Size: 684 4419 3214 Fragments arranged by size: 4419 3214 684 BspHI T'CATG_A Cuts at: 4236 6206 7936 4236 Size: 1970 1730 4617 Fragments arranged by size: 4617 1970 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2399 4467 4498 5672 844 Size: 1555 2068 31 1174 3489 Fragments arranged by size: 3489 2068 1555 1174 31 BsrBI CCG'CTC Cuts at: 1394 1823 2985 4177 4258 4666 4678 6204 1394 Size: 429 1162 1192 81 408 12 1526 3507 Fragments arranged by size: 3507 1526 1192 1162 429 408 81 12 BsrDI GCAATG_nn' Cuts at: 65 1248 2377 2491 2937 5520 6812 6986 65 Size: 1183 1129 114 446 2583 1292 174 1396 Fragments arranged by size: 2583 1396 1292 1183 1129 446 174 114 BsrFI r'CCGG_y Cuts at: 1700 1810 2122 2172 3217 4341 5078 6961 7148 Size: 110 312 50 1045 1124 737 1883 187 Cuts at: 7148 1700 Size: 2869 Fragments arranged by size: 2869 1883 1124 1045 737 312 187 110 50 BsrGI T'GTAC_A Cuts at: 96 5160 5274 96 Size: 5064 114 3139 Fragments arranged by size: 5064 3139 114 BssHII G'CGCG_C Cuts at: 1925 2101 2103 2382 1925 Size: 176 2 279 7860 Fragments arranged by size: 7860 279 176 2 BssSI C'ACGA_G Cuts at: 2309 2468 3721 3758 5489 6377 7389 2309 Size: 159 1253 37 1731 888 1012 3237 Fragments arranged by size: 3237 1731 1253 1012 888 159 37 BstAPI GCAn_nnn'nTGC Cuts at: 794 2857 794 Size: 2063 6254 BstBI TT'CG_AA Cuts at: 2520 7121 2520 Size: 4601 3716 BstEII G'GTnAC_C Cuts at: 1184 3673 3764 4948 1184 Size: 2489 91 1184 4553 Fragments arranged by size: 4553 2489 1184 91 BstXI CCAn_nnnn'nTGG Cuts at: 2824 4609 5338 2824 Size: 1785 729 5803 Fragments arranged by size: 5803 1785 729 BstYI r'GATC_y Cuts at: 1972 2289 2646 2874 3564 4035 4209 4971 5647 Size: 317 357 228 690 471 174 762 676 Cuts at: 5647 6014 6399 6416 7868 7954 7966 8312 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 762 690 676 471 385 367 357 346 317 228 174 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2460 2577 3204 3795 5325 513 Size: 552 939 456 117 627 591 1530 3505 Fragments arranged by size: 3505 1530 939 627 591 552 456 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3355 3505 3937 4855 487 Size: 683 56 2129 150 432 918 3949 Fragments arranged by size: 3949 2129 918 683 432 150 56 BtsI GCAGTG_nn' Cuts at: 4260 4352 5833 6627 6647 4260 Size: 92 1481 794 20 5930 Fragments arranged by size: 5930 1481 794 92 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8317 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8282 DraI TTT'AAA Cuts at: 5621 5621 Size: 8317 DraIII CAC_nnn'GTG Cuts at: 4231 4231 Size: 8317 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3682 7324 809 Size: 507 2366 3642 1802 Fragments arranged by size: 3642 2366 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2165 2980 3501 Size: 54 1425 50 275 18 335 815 521 Cuts at: 3501 3528 3648 3792 3957 4650 5076 5322 5328 Size: 27 120 144 165 693 426 246 6 Cuts at: 5328 5340 5433 5562 5764 6653 8282 8 Size: 12 93 129 202 889 1629 43 Fragments arranged by size: 1629 1425 889 815 693 521 426 335 275 246 202 165 144 129 120 93 54 50 43 27 18 12 6 EagI C'GGCC_G Cuts at: 1537 1812 2980 3792 3957 5764 1537 Size: 275 1168 812 165 1807 4090 Fragments arranged by size: 4090 1807 1168 812 275 165 EarI CTCTTCn'nnn_ Cuts at: 1353 3302 3356 5096 6247 1353 Size: 1949 54 1740 1151 3423 Fragments arranged by size: 3423 1949 1740 1151 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4478 6904 7278 7424 387 Size: 1064 138 2889 2426 374 146 1280 Fragments arranged by size: 2889 2426 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2467 2467 Size: 8317 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2576 3113 3233 3350 3461 3914 4151 Size: 393 944 537 120 117 111 453 237 Cuts at: 4151 4439 4580 4640 4720 5045 5168 6380 6977 Size: 288 141 60 80 325 123 1212 597 Cuts at: 6977 7764 8032 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1212 944 787 597 537 453 393 325 288 268 237 141 123 120 117 111 80 60 EcoNI CCTnn'n_nnAGG Cuts at: 4792 4808 4792 Size: 16 8301 EcoO109I rG'GnC_Cy Cuts at: 2046 3409 3712 3969 3970 4126 5530 5592 2046 Size: 1363 303 257 1 156 1404 62 4771 Fragments arranged by size: 4771 1404 1363 303 257 156 62 1 EcoRI G'AATT_C Cuts at: 2522 5626 6004 2522 Size: 3104 378 4835 Fragments arranged by size: 4835 3104 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2519 2519 Size: 8317 FspI TGC'GCA Cuts at: 3287 6823 3287 Size: 3536 4781 HaeII r_GCGC'y Cuts at: 1678 2129 2186 2197 3874 4067 4360 4617 4646 Size: 451 57 11 1677 193 293 257 29 Cuts at: 4646 4982 5558 7464 1678 Size: 336 576 1906 2531 Fragments arranged by size: 2531 1906 1677 576 451 336 293 257 193 57 29 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 2957 2989 4041 Size: 32 135 32 926 32 427 32 1052 Cuts at: 4041 4073 6958 6990 7032 7064 1373 Size: 32 2885 32 42 32 2626 Fragments arranged by size: 2885 2626 1052 926 427 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3223 3508 3618 5302 5661 5907 Size: 988 281 1285 285 110 1684 359 246 Cuts at: 5907 669 Size: 3079 Fragments arranged by size: 3079 1684 1285 988 359 285 281 246 110 HindIII A'AGCT_T Cuts at: 748 748 Size: 8317 HpaI GTT'AAC Cuts at: 5907 5907 Size: 8317 KasI G'GCGC_C Cuts at: 2193 4613 2193 Size: 2420 5897 KpnI G_GTAC'C Cuts at: 3405 5642 3405 Size: 2237 6080 MfeI C'AATT_G Cuts at: 5916 5916 Size: 8317 MluI A'CGCG_T Cuts at: 8193 8193 Size: 8317 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2850 7431 7615 1305 Size: 1545 4581 184 2007 Fragments arranged by size: 4581 2007 1545 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3530 4652 5324 5330 5342 Size: 54 1425 343 1698 1122 672 6 12 Cuts at: 5342 5435 10 Size: 93 2892 Fragments arranged by size: 2892 1698 1425 1122 672 343 93 54 12 6 MslI CAynn'nnrTG Cuts at: 518 1166 2484 2822 3170 3758 4946 5177 5324 Size: 648 1318 338 348 588 1188 231 147 Cuts at: 5324 5330 5336 5501 6275 6634 6793 518 Size: 6 6 165 774 359 159 2042 Fragments arranged by size: 2042 1318 1188 774 648 588 359 348 338 231 165 159 147 6 6 MspA1I CmG'CkG Cuts at: 1606 2015 2255 2657 3036 3228 3380 3742 4483 Size: 409 240 402 379 192 152 362 741 Cuts at: 4483 4753 4938 5733 6410 7558 7803 1606 Size: 270 185 795 677 1148 245 2120 Fragments arranged by size: 2120 1148 795 741 677 409 402 379 362 270 245 240 192 185 152 NaeI GCC'GGC Cuts at: 2124 4343 5080 2124 Size: 2219 737 5361 Fragments arranged by size: 5361 2219 737 NarI GG'CG_CC Cuts at: 2194 4614 2194 Size: 2420 5897 NcoI C'CATG_G Cuts at: 513 1065 2004 5325 513 Size: 552 939 3321 3505 Fragments arranged by size: 3505 3321 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8317 NgoMIV G'CCGG_C Cuts at: 2122 4341 5078 2122 Size: 2219 737 5361 Fragments arranged by size: 5361 2219 737 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8317 NotI GC'GGCC_GC Cuts at: 5764 5764 Size: 8317 NspI r_CATG'y Cuts at: 1132 1229 2869 3544 4269 4628 5675 7220 1132 Size: 97 1640 675 725 359 1047 1545 2229 Fragments arranged by size: 2229 1640 1545 1047 725 675 359 97 PasI CC'CwG_GG Cuts at: 1348 3271 1348 Size: 1923 6394 PciI A'CATG_T Cuts at: 2865 3540 7216 2865 Size: 675 3676 3966 Fragments arranged by size: 3966 3676 675 PflMI CCAn_nnn'nTGG Cuts at: 1108 4231 1108 Size: 3123 5194 PfoI T'CCnGG_A Cuts at: 2631 4203 4736 5538 2631 Size: 1572 533 802 5410 Fragments arranged by size: 5410 1572 802 533 PmeI GTTT'AAAC Cuts at: 5621 5621 Size: 8317 PmlI CAC'GTG Cuts at: 4273 4921 4273 Size: 648 7669 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6386 6418 7899 7931 6386 Size: 32 1481 32 6772 Fragments arranged by size: 6772 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3409 5530 5592 3409 Size: 2121 62 6134 Fragments arranged by size: 6134 2121 62 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8317 PsiI TTA'TAA Cuts at: 5887 5887 Size: 8317 PspOMI G'GGCC_C Cuts at: 2046 3711 3969 2046 Size: 1665 258 6394 Fragments arranged by size: 6394 1665 258 PspXI vC'TCGA_Gb Cuts at: 4826 4826 Size: 8317 PstI C_TGCA'G Cuts at: 830 3379 4323 4464 5580 5669 830 Size: 2549 944 141 1116 89 3478 Fragments arranged by size: 3478 2549 1116 944 141 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8317 PvuII CAG'CTG Cuts at: 2657 3380 3742 4483 2657 Size: 723 362 741 6491 Fragments arranged by size: 6491 741 723 362 RsrII CG'GwC_CG Cuts at: 3252 3252 Size: 8317 SacI G_AGCT'C Cuts at: 2469 2469 Size: 8317 SalI G'TCGA_C Cuts at: 1936 5659 1936 Size: 3723 4594 SanDI GG'GwC_CC Cuts at: 5592 5592 Size: 8317 SbfI CC_TGCA'GG Cuts at: 4464 5669 4464 Size: 1205 7112 ScaI AGT'ACT Cuts at: 2789 6565 8277 2789 Size: 3776 1712 2829 Fragments arranged by size: 3776 2829 1712 SexAI A'CCwGG_T Cuts at: 3000 3000 Size: 8317 SfcI C'TryA_G Cuts at: 826 1045 2797 3375 3696 4319 4460 4958 5002 Size: 219 1752 578 321 623 141 498 44 Cuts at: 5002 5060 5576 5665 6800 7481 7672 826 Size: 58 516 89 1135 681 191 1471 Fragments arranged by size: 1752 1471 1135 681 623 578 516 498 321 219 191 141 89 58 44 SfoI GGC'GCC Cuts at: 2195 4615 2195 Size: 2420 5897 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8317 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8317 SmaI CCC'GGG Cuts at: 1872 2089 4043 4109 4394 4694 5644 1872 Size: 217 1954 66 285 300 950 4545 Fragments arranged by size: 4545 1954 950 300 285 217 66 SmlI C'TyrA_G Cuts at: 820 1017 1943 2504 4142 4178 4826 5114 6421 Size: 197 926 561 1638 36 648 288 1307 Cuts at: 6421 7322 7584 820 Size: 901 262 1553 Fragments arranged by size: 1638 1553 1307 926 901 648 561 288 262 197 36 SnaBI TAC'GTA Cuts at: 493 6108 493 Size: 5615 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8317 SphI G_CATG'C Cuts at: 1132 4269 4628 5675 1132 Size: 3137 359 1047 3774 Fragments arranged by size: 3774 3137 1047 359 SrfI GCCC'GGGC Cuts at: 2089 2089 Size: 8317 SspI AAT'ATT Cuts at: 5 52 6241 5 Size: 47 6189 2081 Fragments arranged by size: 6189 2081 47 StuI AGG'CCT Cuts at: 1517 2837 1517 Size: 1320 6997 StyI C'CwwG_G Cuts at: 513 1065 2004 3168 3405 4191 5325 5756 513 Size: 552 939 1164 237 786 1134 431 3074 Fragments arranged by size: 3074 1164 1134 939 786 552 431 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 2272 3454 3752 4625 6344 6361 Size: 1255 651 41 1182 298 873 1719 17 Cuts at: 6361 6514 6699 7144 325 Size: 153 185 445 1498 Fragments arranged by size: 1719 1498 1255 1182 873 651 445 298 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2787 3543 5160 Size: 274 80 33 51 1443 810 756 1617 Cuts at: 5160 5274 6563 8275 96 Size: 114 1289 1712 138 Fragments arranged by size: 1712 1617 1443 1289 810 756 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8277 8309 8277 Size: 32 8285 Tth111I GACn'n_nGTC Cuts at: 1316 2969 5570 1316 Size: 1653 2601 4063 Fragments arranged by size: 4063 2601 1653 XbaI T'CTAG_A Cuts at: 3772 5653 3772 Size: 1881 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2467 3628 4852 2467 Size: 1161 1224 5932 Fragments arranged by size: 5932 1224 1161 XhoI C'TCGA_G Cuts at: 1943 4826 1943 Size: 2883 5434 XmaI C'CCGG_G Cuts at: 1870 2087 4041 4107 4392 4692 5642 1870 Size: 217 1954 66 285 300 950 4545 Fragments arranged by size: 4545 1954 950 300 285 217 66 XmnI GAAnn'nnTTC Cuts at: 3827 6446 3827 Size: 2619 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6125 276 Size: 53 83 186 936 4591 2468 Fragments arranged by size: 4591 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PsrI SacII SapI SfiI SwaI