Restriction map for
pFN21AA1675
(Linear) (Six-base) MAPSORT of: KIAA1675.seq Check: 6041 from: 1 to: 3828
KIAA1675.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AatII G_ACGT'C
Cuts at: 0 1820 3828
Size: 1820 2008
AccI GT'mk_AC
Cuts at: 0 215 641 3828
Size: 215 426 3187
Fragments arranged by size:
3187 426 215
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1039 1792 2665 2946 3828
Size: 245 794 753 873 281 882
Fragments arranged by size:
882 873 794 753 281 245
AflIII A'CryG_T
Cuts at: 0 752 754 1790 2852 3828
Size: 752 2 1036 1062 976
Fragments arranged by size:
1062 1036 976 752 2
AhdI GACnn_n'nnGTC
Cuts at: 0 3426 3828
Size: 3426 402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2779 3828
Size: 2779 1049
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 670 702 3828
Size: 670 32 3126
Fragments arranged by size:
3126 670 32
AlwNI CAG_nnn'CTG
Cuts at: 0 280 2046 2187 2406 2864 3828
Size: 280 1766 141 219 458 964
Fragments arranged by size:
1766 964 458 280 219 141
ApaI G_GGCC'C
Cuts at: 0 334 1095 1724 1974 2374 2433 3828
Size: 334 761 629 250 400 59 1395
Fragments arranged by size:
1395 761 629 400 334 250 59
ApaLI G'TGCA_C
Cuts at: 0 622 3828
Size: 622 3206
AvaI C'yCGr_G
Cuts at: 0 350 808 1088 1374 1482 1542 1723 1965
Size: 350 458 280 286 108 60 181 242
Cuts at: 1965 2273 2751 3121 3439 3828
Size: 308 478 370 318 389
Fragments arranged by size:
478 458 389 370 350 318 308 286
280 242 181 108 60
BanI G'GyrC_C
Cuts at: 0 345 737 1506 1959 1992 2059 2500 3074
Size: 345 392 769 453 33 67 441 574
Cuts at: 3074 3828
Size: 754
Fragments arranged by size:
769 754 574 453 441 392 345 67
33
BanII G_rGCy'C
Cuts at: 0 71 196 257 334 683 803 1095 1612
Size: 71 125 61 77 349 120 292 517
Cuts at: 1612 1724 1867 1974 2152 2232 2374 2433 2465
Size: 112 143 107 178 80 142 59 32
Cuts at: 2465 2643 3446 3828
Size: 178 803 382
Fragments arranged by size:
803 517 382 349 292 178 178 143
142 125 120 112 107 80 77 71
61 59 32
BbeI G_GCGC'C
Cuts at: 0 741 1510 1963 2063 2504 3828
Size: 741 769 453 100 441 1324
Fragments arranged by size:
1324 769 741 453 441 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 233 452 1123 1780 1992 3212 3731 3828
Size: 233 219 671 657 212 1220 519 97
Fragments arranged by size:
1220 671 657 519 233 219 212 97
BbvCI CC'TCA_GC
Cuts at: 0 794 3527 3605 3828
Size: 794 2733 78 223
Fragments arranged by size:
2733 794 223 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 103 137 990 1024 2837 2871 3828
Size: 103 34 853 34 1813 34 957
Fragments arranged by size:
1813 957 853 103 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2447 2743 3493 3828
Size: 2447 296 750 335
Fragments arranged by size:
2447 750 335 296
BglII A'GATC_T
Cuts at: 0 437 2873 3828
Size: 437 2436 955
Fragments arranged by size:
2436 955 437
Bme1580I G_kGCm'C
Cuts at: 0 334 350 626 655 1095 1705 1724 1974
Size: 334 16 276 29 440 610 19 250
Cuts at: 1974 2314 2374 2433 2530 3315 3828
Size: 340 60 59 97 785 513
Fragments arranged by size:
785 610 513 440 340 334 276 250
97 60 59 29 19 16
BmgBI CAC'GTC
Cuts at: 0 402 1130 1884 2343 2478 2991 3351 3791
Size: 402 728 754 459 135 513 360 440
Cuts at: 3791 3828
Size: 37
Fragments arranged by size:
754 728 513 459 440 402 360 135
37
BmrI ACTGGGnnnn_n'
Cuts at: 0 1637 2294 2930 3309 3828
Size: 1637 657 636 379 519
Fragments arranged by size:
1637 657 636 519 379
BmtI G_CTAG'C
Cuts at: 0 31 3828
Size: 31 3797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1455 1768 2743 2965 3397 3803 3821
Size: 907 548 313 975 222 432 406 18
Cuts at: 3821 3828
Size: 7
Fragments arranged by size:
975 907 548 432 406 313 222 18
7
Bpu10I CC'TnA_GC
Cuts at: 0 794 2413 3527 3605 3828
Size: 794 1619 1114 78 223
Fragments arranged by size:
1619 1114 794 223 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 787 1126 1588 2269 3064 3828
Size: 490 297 339 462 681 795 764
Fragments arranged by size:
795 764 681 490 462 339 297
BsaI GGTCTCn'nnnn_
Cuts at: 0 1680 2698 3828
Size: 1680 1018 1130
Fragments arranged by size:
1680 1130 1018
BsaAI yAC'GTr
Cuts at: 0 627 755 1080 2855 3828
Size: 627 128 325 1775 973
Fragments arranged by size:
1775 973 627 325 128
BsaBI GATnn'nnATC
Cuts at: 0 479 3828
Size: 479 3349
BsaHI Gr'CG_yC
Cuts at: 0 638 738 1507 1631 1817 1960 2060 2279
Size: 638 100 769 124 186 143 100 219
Cuts at: 2279 2501 3828
Size: 222 1327
Fragments arranged by size:
1327 769 638 222 219 186 143 124
100 100
BsaWI w'CCGG_w
Cuts at: 0 2795 2993 3828
Size: 2795 198 835
Fragments arranged by size:
2795 835 198
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 670 700 907 937 1453 1483 1494 1524
Size: 670 30 207 30 516 30 11 30
Cuts at: 1524 2917 2947 3730 3760 3828
Size: 1393 30 783 30 68
Fragments arranged by size:
1393 783 670 516 207 68 30 30
30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 691 811 928 1296 1741 2410 3370
Size: 625 66 120 117 368 445 669 960
Cuts at: 3370 3373 3394 3481 3490 3722 3828
Size: 3 21 87 9 232 106
Fragments arranged by size:
960 669 625 445 368 232 120 117
106 87 66 21 9 3
BseYI C'CCAG_C
Cuts at: 0 240 327 1045 1393 1426 1659 2009 2145
Size: 240 87 718 348 33 233 350 136
Cuts at: 2145 2496 2543 3828
Size: 351 47 1285
Fragments arranged by size:
1285 718 351 350 348 240 233 136
87 47 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2877 3696 3828
Size: 2877 819 132
Fragments arranged by size:
2877 819 132
BsiEI CG_ry'CG
Cuts at: 0 1576 2665 2955 3828
Size: 1576 1089 290 873
Fragments arranged by size:
1576 1089 873 290
BsiHKAI G_wGCw'C
Cuts at: 0 257 529 626 803 3095 3828
Size: 257 272 97 177 2292 733
Fragments arranged by size:
2292 733 272 257 177 97
BsiWI C'GTAC_G
Cuts at: 0 200 3828
Size: 200 3628
Bsp1286I G_dGCh'C
Cuts at: 0 71 196 257 334 350 529 626 655
Size: 71 125 61 77 16 179 97 29
Cuts at: 655 683 803 1095 1612 1705 1724 1867 1974
Size: 28 120 292 517 93 19 143 107
Cuts at: 1974 2152 2232 2314 2374 2433 2465 2530 2643
Size: 178 80 82 60 59 32 65 113
Cuts at: 2643 3095 3315 3446 3828
Size: 452 220 131 382
Fragments arranged by size:
517 452 382 292 220 179 178 143
131 125 120 113 107 97 93 82
80 77 71 65 61 60 59 32
29 28 19 16
BspEI T'CCGG_A
Cuts at: 0 2993 3828
Size: 2993 835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 740 2666 2849 3828
Size: 89 651 1926 183 979
Fragments arranged by size:
1926 979 651 183 89
BsrBI CCG'CTC
Cuts at: 0 861 1070 2556 2957 3828
Size: 861 209 1486 401 871
Fragments arranged by size:
1486 871 861 401 209
BsrDI GCAATG_nn'
Cuts at: 0 2093 3710 3828
Size: 2093 1617 118
Fragments arranged by size:
2093 1617 118
BsrFI r'CCGG_y
Cuts at: 0 1229 1322 2503 3071 3828
Size: 1229 93 1181 568 757
Fragments arranged by size:
1229 1181 757 568 93
BssSI C'ACGA_G
Cuts at: 0 841 3088 3094 3828
Size: 841 2247 6 734
Fragments arranged by size:
2247 841 734 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 854 2095 3044 3828
Size: 854 1241 949 784
Fragments arranged by size:
1241 949 854 784
BstBI TT'CG_AA
Cuts at: 0 1010 2974 3828
Size: 1010 1964 854
Fragments arranged by size:
1964 1010 854
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 1650 2482 3828
Size: 141 1509 832 1346
Fragments arranged by size:
1509 1346 832 141
BstYI r'GATC_y
Cuts at: 0 437 2873 3828
Size: 437 2436 955
Fragments arranged by size:
2436 955 437
Bsu36I CC'TnA_GG
Cuts at: 0 2830 2908 3828
Size: 2830 78 920
Fragments arranged by size:
2830 920 78
BtgI C'CryG_G
Cuts at: 0 140 1502 1829 1853 2633 3497 3828
Size: 140 1362 327 24 780 864 331
Fragments arranged by size:
1362 864 780 331 327 140 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 548 2070 3828
Size: 548 1522 1758
Fragments arranged by size:
1758 1522 548
BtsI GCAGTG_nn'
Cuts at: 0 462 1809 3315 3828
Size: 462 1347 1506 513
Fragments arranged by size:
1506 1347 513 462
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1263 1298 3828
Size: 1263 35 2530
Fragments arranged by size:
2530 1263 35
DraI TTT'AAA
Cuts at: 0 3662 3828
Size: 3662 166
DraIII CAC_nnn'GTG
Cuts at: 0 554 3828
Size: 554 3274
DrdI GACnn_nn'nnGTC
Cuts at: 0 3153 3828
Size: 3153 675
EaeI y'GGCC_r
Cuts at: 0 86 831 1284 1573 1985 2662 3401 3494
Size: 86 745 453 289 412 677 739 93
Cuts at: 3494 3828
Size: 334
Fragments arranged by size:
745 739 677 453 412 334 289 93
86
EagI C'GGCC_G
Cuts at: 0 1573 2662 3828
Size: 1573 1089 1166
Fragments arranged by size:
1573 1166 1089
EarI CTCTTCn'nnn_
Cuts at: 0 718 2683 2976 3828
Size: 718 1965 293 852
Fragments arranged by size:
1965 852 718 293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 964 1668 1914 2761 3301 3828
Size: 964 704 246 847 540 527
Fragments arranged by size:
964 847 704 540 527 246
EcoICRI GAG'CTC
Cuts at: 0 255 801 3828
Size: 255 546 3027
Fragments arranged by size:
3027 546 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 907 1039 1455 1768 1792 2665 2743
Size: 245 662 132 416 313 24 873 78
Cuts at: 2743 2946 2965 3397 3803 3821 3828
Size: 203 19 432 406 18 7
Fragments arranged by size:
873 662 432 416 406 313 245 203
132 78 24 19 18 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 673 793 2833 3379 3828
Size: 673 120 2040 546 449
Fragments arranged by size:
2040 673 546 449 120
EcoO109I rG'GnC_Cy
Cuts at: 0 78 207 331 786 813 1720 1970 1971
Size: 78 129 124 455 27 907 250 1
Cuts at: 1971 2370 2371 3126 3828
Size: 399 1 755 702
Fragments arranged by size:
907 755 702 455 399 250 129 124
78 27 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3649 3828
Size: 3649 179
HaeII r_GCGC'y
Cuts at: 0 741 1088 1329 1510 1963 2063 2504 3828
Size: 741 347 241 181 453 100 441 1324
Fragments arranged by size:
1324 741 453 441 347 241 181 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2251 2283 3412 3444 3828
Size: 2251 32 1129 32 384
Fragments arranged by size:
2251 1129 384 32 32
HincII GTy'rAC
Cuts at: 0 1821 3828
Size: 1821 2007
KasI G'GCGC_C
Cuts at: 0 737 1506 1959 2059 2500 3828
Size: 737 769 453 100 441 1328
Fragments arranged by size:
1328 769 737 453 441 100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 989 1244 1775 2843 3155 3773 3828
Size: 989 255 531 1068 312 618 55
Fragments arranged by size:
1068 989 618 531 312 255 55
MscI TGG'CCA
Cuts at: 0 88 1286 3828
Size: 88 1198 2542
Fragments arranged by size:
2542 1198 88
MslI CAynn'nnrTG
Cuts at: 0 139 517 2128 2480 3828
Size: 139 378 1611 352 1348
Fragments arranged by size:
1611 1348 378 352 139
MspA1I CmG'CkG
Cuts at: 0 500 692 830 998 1059 1599 1714 2043
Size: 500 192 138 168 61 540 115 329
Cuts at: 2043 2406 2673 2985 3044 3604 3828
Size: 363 267 312 59 560 224
Fragments arranged by size:
560 540 500 363 329 312 267 224
192 168 138 115 61 59
NaeI GCC'GGC
Cuts at: 0 1231 2505 3828
Size: 1231 1274 1323
Fragments arranged by size:
1323 1274 1231
NarI GG'CG_CC
Cuts at: 0 738 1507 1960 2060 2501 3828
Size: 738 769 453 100 441 1327
Fragments arranged by size:
1327 769 738 453 441 100
NdeI CA'TA_TG
Cuts at: 0 1183 3699 3828
Size: 1183 2516 129
Fragments arranged by size:
2516 1183 129
NgoMIV G'CCGG_C
Cuts at: 0 1229 2503 3828
Size: 1229 1274 1325
Fragments arranged by size:
1325 1274 1229
NheI G'CTAG_C
Cuts at: 0 27 3828
Size: 27 3801
NspI r_CATG'y
Cuts at: 0 1794 3294 3828
Size: 1794 1500 534
Fragments arranged by size:
1794 1500 534
PasI CC'CwG_GG
Cuts at: 0 274 648 1650 3750 3828
Size: 274 374 1002 2100 78
Fragments arranged by size:
2100 1002 374 274 78
PciI A'CATG_T
Cuts at: 0 1790 3828
Size: 1790 2038
PflMI CCAn_nnn'nTGG
Cuts at: 0 280 3828
Size: 280 3548
PmlI CAC'GTG
Cuts at: 0 627 755 1080 2855 3828
Size: 627 128 325 1775 973
Fragments arranged by size:
1775 973 627 325 128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 850 882 1717 1749 3828
Size: 670 32 148 32 835 32 2079
Fragments arranged by size:
2079 835 670 148 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 78 3126 3828
Size: 78 3048 702
Fragments arranged by size:
3048 702 78
PshAI GACnn'nnGTC
Cuts at: 0 938 3828
Size: 938 2890
PspOMI G'GGCC_C
Cuts at: 0 330 1091 1720 1970 2370 2429 3828
Size: 330 761 629 250 400 59 1399
Fragments arranged by size:
1399 761 629 400 330 250 59
PstI C_TGCA'G
Cuts at: 0 2768 3828
Size: 2768 1060
PvuII CAG'CTG
Cuts at: 0 500 1714 2043 3604 3828
Size: 500 1214 329 1561 224
Fragments arranged by size:
1561 1214 500 329 224
RsrII CG'GwC_CG
Cuts at: 0 2223 3828
Size: 2223 1605
SacI G_AGCT'C
Cuts at: 0 257 803 3828
Size: 257 546 3025
Fragments arranged by size:
3025 546 257
SapI GCTCTTCn'nnn_
Cuts at: 0 2683 3828
Size: 2683 1145
ScaI AGT'ACT
Cuts at: 0 1462 2107 2947 3828
Size: 1462 645 840 881
Fragments arranged by size:
1462 881 840 645
SfcI C'TryA_G
Cuts at: 0 2598 2764 3828
Size: 2598 166 1064
Fragments arranged by size:
2598 1064 166
SfoI GGC'GCC
Cuts at: 0 739 1508 1961 2061 2502 3828
Size: 739 769 453 100 441 1326
Fragments arranged by size:
1326 769 739 453 441 100
SgrAI Cr'CCGG_yG
Cuts at: 0 1322 2503 3828
Size: 1322 1181 1325
Fragments arranged by size:
1325 1322 1181
SmaI CCC'GGG
Cuts at: 0 352 1376 1484 1544 1967 2275 3123 3828
Size: 352 1024 108 60 423 308 848 705
Fragments arranged by size:
1024 848 705 423 352 308 108 60
SmlI C'TyrA_G
Cuts at: 0 505 802 1141 1603 2284 3079 3828
Size: 505 297 339 462 681 795 749
Fragments arranged by size:
795 749 681 505 462 339 297
StuI AGG'CCT
Cuts at: 0 949 3326 3828
Size: 949 2377 502
Fragments arranged by size:
2377 949 502
StyI C'CwwG_G
Cuts at: 0 8 185 816 944 2366 3828
Size: 8 177 631 128 1422 1462
Fragments arranged by size:
1462 1422 631 177 128 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1639 1852 2240 3828
Size: 1639 213 388 1588
Fragments arranged by size:
1639 1588 388 213
TatI w'GTAC_w
Cuts at: 0 509 749 878 923 1460 2105 2945 3828
Size: 509 240 129 45 537 645 840 883
Fragments arranged by size:
883 840 645 537 509 240 129 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 970 1002 1494 1526 3730 3762 3828
Size: 970 32 492 32 2204 32 66
Fragments arranged by size:
2204 970 492 66 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 1401 1942 3828
Size: 306 1095 541 1886
Fragments arranged by size:
1886 1095 541 306
XmaI C'CCGG_G
Cuts at: 0 350 1374 1482 1542 1965 2273 3121 3828
Size: 350 1024 108 60 423 308 848 707
Fragments arranged by size:
1024 848 707 423 350 308 108 60
XmnI GAAnn'nnTTC
Cuts at: 0 230 1033 1120 1177 2875 3019 3828
Size: 230 803 87 57 1698 144 809
Fragments arranged by size:
1698 809 803 230 144 87 57
ZraI GAC'GTC
Cuts at: 0 1818 3828
Size: 1818 2010
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BglI BglII Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NspI
PasI PciI PflMI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuII RsrII SacI SapI ScaI SfcI SfoI
SgrAI SmaI SmlI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BfrBI BlpI BplI BsmI BsmBI BspHI BsrGI BssHII
BstEII BstZ17I ClaI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NcoI NotI NruI
NsiI PacI PfoI PmeI PsiI PspXI PsrI PvuI
SacII SalI SanDI SbfI SexAI SfiI SgfI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI