Restriction map for
pFN21AA1520
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI AocI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BamHI BbrPI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsiWI BsmBI BspDI BsrDI BssHII
Bst1107I BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI HpaI KpnI
KspI MaeI MamI MfeI MluI
MseI MunI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tru9I Tsp509I XbaI
XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 1393 GTCTAC GT^[AC][GT]AC
AccIII 1133 TCCGGA T^CCGGA
AflIII 1226 ACATGT A^C[GA][CT]GT
Alw44I 1468 GTGCAC G^TGCAC
Aor51HI 2011 AGCGCT AGC^GCT
ApaLI 1468 GTGCAC G^TGCAC
Asp700I 1431 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 892 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 320 TGCGCA TGC^GCA
BbeI 1918 GGCGCC GGCGC^C
BbvCI 463 GCTGAGG CCTCAGC(-5/-2)
BlpI 2100 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2100 GCTGAGC GC^T[ACGT]AGC
BsaWI 1133 TCCGGA [AT]^CCGG[AT]
BseAI 1133 TCCGGA T^CCGGA
BsmI 1074 GAATGC GAATGC(1/-1)
Bsp1407I 1193 TGTACA T^GTACA
BspEI 1133 TCCGGA T^CCGGA
BspLU11I 1226 ACATGT A^CATGT
BsrGI 1193 TGTACA T^GTACA
BstAPI 522 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 782 TTCGAA TT^CGAA
BstEII 990 GGTCACC G^GT[ACGT]ACC
BstPI 990 GGTCACC G^GT[ACGT]ACC
BstYI 144 GGATCT [GA]^GATC[CT]
CelII 2100 GCTGAGC GC^T[ACGT]AGC
DraIII 1735 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 483 CGGCCG C^GGCCG
EclXI 483 CGGCCG C^GGCCG
Eco47III 2011 AGCGCT AGC^GCT
Eco52I 483 CGGCCG C^GGCCG
EcoO65I 990 GGTCACC G^GT[ACGT]ACC
FseI 484 GGCCGGCC GGCCGG^CC
FspI 320 TGCGCA TGC^GCA
HgaI 505 GCGTC GACGC(5/10)
HincII 1645 GTCAAC GT[CT]^[GA]AC
HindII 1645 GTCAAC GT[CT]^[GA]AC
HindIII 822 AAGCTT A^AGCTT
KasI 1918 GGCGCC G^GCGCC
MflI 144 GGATCT [GA]^GATC[CT]
MroI 1133 TCCGGA T^CCGGA
NaeI 485 GCCGGC GCC^GGC
NarI 1918 GGCGCC GG^CGCC
NgoMIV 485 GCCGGC G^CCGGC
NspI 1226 ACATGT [GA]CATG^[CT]
NspV 782 TTCGAA TT^CGAA
PflFI 892 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 432 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1635 GAGCTC GAGCT^C
SexAI 893 ACCTGGT A^CC[AT]GGT
SfoI 1918 GGCGCC GGC^GCC
SfuI 782 TTCGAA TT^CGAA
SmaI 1961 CCCGGG CCC^GGG
SspBI 1193 TGTACA T^GTACA
Tth111I 892 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 432 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 144 GGATCT [GA]^GATC[CT]
XmaCI 1961 CCCGGG C^CCGGG
XmaI 1961 CCCGGG C^CCGGG
XmnI 1431 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcyI 504 GGCGTC G[GA]^CG[CT]C
1918 GGCGCC
BglI 225 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
454 GCCACCGAGGC
BsaHI 504 GGCGTC G[GA]^CG[CT]C
1918 GGCGCC
BsgI 2155 GTGCAG GTGCAG(16/14)
2212 GTGCAG
BsiEI 483 CGGCCG CG[GA][CT]^CG
1449 CGACCG
BspHI 38 TCATGA T^CATGA
1016 TCATGA
BsrI 804 ACTGG ACTGG(1/-1)
1984 CCAGT
BstXI 194 CCATTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2009 CCAGCGCTTTGG
EcoNI 99 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1404 CCTGATGCAGG
Hin1I 504 GGCGTC G[GA]^CG[CT]C
1918 GGCGCC
HinfI 133 GACTC G^A[ACGT]TC
1378 GAGTC
PleI 133 GACTC GAGTC(4/5)
1378 GAGTC
RcaI 38 TCATGA T^CATGA
1016 TCATGA
SapI 1632 GAAGAGC GCTCTTC(1/4)
306 GCTCTTC
ScaI 1043 AGTACT AGT^ACT
1727 AGTACT
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BcnI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI