Restriction map for pFN21AA1520
 (Linear) (Six-base) MAPSORT of: KIAA1520.seq  Check: 7260  from: 1  to: 2298



KIAA1520.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1179    2298 
   Size:       1179    1119
 
AccI GT'mk_AC

Cuts at:      0    1394    2298 
   Size:       1394     904
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63    1563    2218    2298 
   Size:         63    1500     655      80

  Fragments arranged by size: 

                1500     655      80      63
 
AfeI AGC'GCT

Cuts at:      0    2013    2298 
   Size:       2013     285
 
AflIII A'CryG_T

Cuts at:      0    1226    2298 
   Size:       1226    1072
 
AleI CACnn'nnGTG

Cuts at:      0     262     946    2298 
   Size:        262     684    1352

  Fragments arranged by size: 

                1352     684     262
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     193    2298 
   Size:        193    2105
 
AlwNI CAG_nnn'CTG

Cuts at:      0     438     528    1563    2178    2181    2226    2298 
   Size:        438      90    1035     615       3      45      72

  Fragments arranged by size: 

                1035     615     438      90      72      45       3
 
ApaI G_GGCC'C

Cuts at:      0     227     354    1619    2298 
   Size:        227     127    1265     679

  Fragments arranged by size: 

                1265     679     227     127
 
ApaLI G'TGCA_C

Cuts at:      0    1468    2298 
   Size:       1468     830
 
AvaI C'yCGr_G

Cuts at:      0     496    1620    1961    2298 
   Size:        496    1124     341     337

  Fragments arranged by size: 

                1124     496     341     337
 
BanI G'GyrC_C

Cuts at:      0     427    1918    2164    2298 
   Size:        427    1491     246     134

  Fragments arranged by size: 

                1491     427     246     134
 
BanII G_rGCy'C

Cuts at:      0     227     354    1253    1390    1619    1639    1766    1968 
   Size:        227     127     899     137     229      20     127     202

Cuts at:   1968    2298 
   Size:        330

  Fragments arranged by size: 

                 899     330     229     227     202     137     127     127
                  20
 
BbeI G_GCGC'C

Cuts at:      0    1922    2298 
   Size:       1922     376
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      69     909     948    1132    2298 
   Size:         69     840      39     184    1166

  Fragments arranged by size: 

                1166     840     184      69      39
 
BbvCI CC'TCA_GC

Cuts at:      0     464    2298 
   Size:        464    1834
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     666     700    1317    1351    1449    1483    2298 
   Size:        666      34     617      34      98      34     815

  Fragments arranged by size: 

                 815     666     617      98      34      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     231     460    2298 
   Size:        231     229    1838

  Fragments arranged by size: 

                1838     231     229
 
BlpI GC'TnA_GC

Cuts at:      0    2101    2298 
   Size:       2101     197
 
Bme1580I G_kGCm'C

Cuts at:      0     227     354    1472    1619    2167    2298 
   Size:        227     127    1118     147     548     131

  Fragments arranged by size: 

                1118     548     227     147     131     127
 
BmgBI CAC'GTC

Cuts at:      0     552    2298 
   Size:        552    1746
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1978    2298 
   Size:       1978     320
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     460    1519    1675    1693    1867    2298 
   Size:        124     336    1059     156      18     174     431

  Fragments arranged by size: 

                1059     431     336     174     156     124      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     464    2298 
   Size:        464    1834
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     598     903    1102    2298 
   Size:        598     305     199    1196

  Fragments arranged by size: 

                1196     598     305     199
 
BsaHI Gr'CG_yC

Cuts at:      0     505    1919    2298 
   Size:        505    1414     379

  Fragments arranged by size: 

                1414     505     379
 
BsaWI w'CCGG_w

Cuts at:      0    1133    2298 
   Size:       1133    1165
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     841     871     883     913    2298 
   Size:        841      30      12      30    1385

  Fragments arranged by size: 

                1385     841      30      30      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1120    1165    1168    1171    2298 
   Size:       1120      45       3       3    1127

  Fragments arranged by size: 

                1127    1120      45       3       3
 
BseYI C'CCAG_C

Cuts at:      0     183     219    1922    2226    2298 
   Size:        183      36    1703     304      72

  Fragments arranged by size: 

                1703     304     183      72      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2176    2233    2298 
   Size:       2176      57      65

  Fragments arranged by size: 

                2176      65      57
 
BsiEI CG_ry'CG

Cuts at:      0     486    1452    2298 
   Size:        486     966     846

  Fragments arranged by size: 

                 966     846     486
 
BsiHKAI G_wGCw'C

Cuts at:      0    1472    1639    2107    2117    2298 
   Size:       1472     167     468      10     181

  Fragments arranged by size: 

                1472     468     181     167      10
 
BsmI GAATG_Cn'

Cuts at:      0    1080    2298 
   Size:       1080    1218
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     354    1253    1390    1472    1619    1639    1766 
   Size:        227     127     899     137      82     147      20     127

Cuts at:   1766    1968    2107    2117    2167    2298 
   Size:        202     139      10      50     131

  Fragments arranged by size: 

                 899     227     202     147     139     137     131     127
                 127      82      50      20      10
 
BspEI T'CCGG_A

Cuts at:      0    1133    2298 
   Size:       1133    1165
 
BspHI T'CATG_A

Cuts at:      0      38    1016    2298 
   Size:         38     978    1282

  Fragments arranged by size: 

                1282     978      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     718    1179    1181    2072    2298 
   Size:        718     461       2     891     226

  Fragments arranged by size: 

                 891     718     461     226       2
 
BsrBI CCG'CTC

Cuts at:      0     482     800    1781    2298 
   Size:        482     318     981     517

  Fragments arranged by size: 

                 981     517     482     318
 
BsrFI r'CCGG_y

Cuts at:      0     485    1451    2096    2298 
   Size:        485     966     645     202

  Fragments arranged by size: 

                 966     645     485     202
 
BsrGI T'GTAC_A

Cuts at:      0    1193    2298 
   Size:       1193    1105
 
BssSI C'ACGA_G

Cuts at:      0     243     262     562    2298 
   Size:        243      19     300    1736

  Fragments arranged by size: 

                1736     300     243      19
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     528    2298 
   Size:        528    1770
 
BstBI TT'CG_AA

Cuts at:      0     783    2298 
   Size:        783    1515
 
BstEII G'GTnAC_C

Cuts at:      0     990    2298 
   Size:        990    1308
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     201    2016    2298 
   Size:        201    1815     282

  Fragments arranged by size: 

                1815     282     201
 
BstYI r'GATC_y

Cuts at:      0     144    2298 
   Size:        144    2154
 
BtgI C'CryG_G

Cuts at:      0      62     290     881    1382    1463    1863    1955    2055 
   Size:         62     228     591     501      81     400      92     100

Cuts at:   2055    2108    2298 
   Size:         53     190

  Fragments arranged by size: 

                 591     501     400     228     190     100      92      81
                  62      53
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1320    2073    2298 
   Size:       1320     753     225

  Fragments arranged by size: 

                1320     753     225
 
BtsI GCAGTG_nn'

Cuts at:      0    1255    1632    2298 
   Size:       1255     377     666

  Fragments arranged by size: 

                1255     666     377
 
DraIII CAC_nnn'GTG

Cuts at:      0    1740    2298 
   Size:       1740     558
 
EaeI y'GGCC_r

Cuts at:      0      65     203     421     452     483     487     566     704 
   Size:         65     138     218      31      31       4      79     138

Cuts at:    704    1082    1935    1952    2259    2298 
   Size:        378     853      17     307      39

  Fragments arranged by size: 

                 853     378     307     218     138     138      79      65
                  39      31      31      17       4
 
EagI C'GGCC_G

Cuts at:      0     483    2298 
   Size:        483    1815
 
EarI CTCTTCn'nnn_

Cuts at:      0     277     313     799    1627    2298 
   Size:        277      36     486     828     671

  Fragments arranged by size: 

                 828     671     486     277      36
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     103    1117    2298 
   Size:        103    1014    1181

  Fragments arranged by size: 

                1181    1014     103
 
EcoICRI GAG'CTC

Cuts at:      0    1637    2298 
   Size:       1637     661
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     124     460    1519    1563    1675    1693    1867 
   Size:         63      61     336    1059      44     112      18     174

Cuts at:   1867    2218    2298 
   Size:        351      80

  Fragments arranged by size: 

                1059     351     336     174     112      80      63      61
                  44      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     103    1408    2298 
   Size:        103    1305     890

  Fragments arranged by size: 

                1305     890     103
 
EcoO109I rG'GnC_Cy

Cuts at:      0     223     224     336     351     435     849    1560    1615 
   Size:        223       1     112      15      84     414     711      55

Cuts at:   1615    1616    2298 
   Size:          1     682

  Fragments arranged by size: 

                 711     682     414     223     112      84      55      15
                   1       1
 
FseI GG_CCGG'CC

Cuts at:      0     489    2298 
   Size:        489    1809
 
FspI TGC'GCA

Cuts at:      0     322    2298 
   Size:        322    1976
 
HaeII r_GCGC'y

Cuts at:      0    1715    1922    2015    2298 
   Size:       1715     207      93     283

  Fragments arranged by size: 

                1715     283     207      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     490     522     841     873     883     915    1783    1815 
   Size:        490      32     319      32      10      32     868      32

Cuts at:   1815    2019    2051    2298 
   Size:        204      32     247

  Fragments arranged by size: 

                 868     490     319     247     204      32      32      32
                  32      32      10
 
HincII GTy'rAC

Cuts at:      0    1647    2298 
   Size:       1647     651
 
HindIII A'AGCT_T

Cuts at:      0     822    2298 
   Size:        822    1476
 
KasI G'GCGC_C

Cuts at:      0    1918    2298 
   Size:       1918     380
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1052    2298 
   Size:       1052    1246
 
MscI TGG'CCA

Cuts at:      0     205     706    1084    1937    1954    2261    2298 
   Size:        205     501     378     853      17     307      37

  Fragments arranged by size: 

                 853     501     378     307     205      37      17
 
MslI CAynn'nnrTG

Cuts at:      0      43      55     199     262     880     946     997    1015 
   Size:         43      12     144      63     618      66      51      18

Cuts at:   1015    1018    1021    2131    2298 
   Size:          3       3    1110     167

  Fragments arranged by size: 

                1110     618     167     144      66      63      51      43
                  18      12       3       3
 
MspA1I CmG'CkG

Cuts at:      0     173    1219    1247    1325    1926    1947    2178    2217 
   Size:        173    1046      28      78     601      21     231      39

Cuts at:   2217    2257    2298 
   Size:         40      41

  Fragments arranged by size: 

                1046     601     231     173      78      41      40      39
                  28      21
 
NaeI GCC'GGC

Cuts at:      0     487    2298 
   Size:        487    1811
 
NarI GG'CG_CC

Cuts at:      0    1919    2298 
   Size:       1919     379
 
NcoI C'CATG_G

Cuts at:      0     290     881    1463    1955    2055    2298 
   Size:        290     591     582     492     100     243

  Fragments arranged by size: 

                 591     582     492     290     243     100
 
NgoMIV G'CCGG_C

Cuts at:      0     485    2298 
   Size:        485    1813
 
NspI r_CATG'y

Cuts at:      0    1230    2298 
   Size:       1230    1068
 
PasI CC'CwG_GG

Cuts at:      0     475    2187    2298 
   Size:        475    1712     111

  Fragments arranged by size: 

                1712     475     111
 
PciI A'CATG_T

Cuts at:      0    1226    2298 
   Size:       1226    1072
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     438    2298 
   Size:        438    1860
 
PfoI T'CCnGG_A

Cuts at:      0     101     332    1652    1883    1994    2298 
   Size:        101     231    1320     231     111     304

  Fragments arranged by size: 

                1320     304     231     231     111     101
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1420    1452    2298 
   Size:       1420      32     846

  Fragments arranged by size: 

                1420     846      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     336     849    1560    2298 
   Size:        336     513     711     738

  Fragments arranged by size: 

                 738     711     513     336
 
PspOMI G'GGCC_C

Cuts at:      0     223     350    1615    2298 
   Size:        223     127    1265     683

  Fragments arranged by size: 

                1265     683     223     127
 
PstI C_TGCA'G

Cuts at:      0     524    1187    1568    2069    2298 
   Size:        524     663     381     501     229

  Fragments arranged by size: 

                 663     524     501     381     229
 
PvuII CAG'CTG

Cuts at:      0     173    1219    1926    2178    2257    2298 
   Size:        173    1046     707     252      79      41

  Fragments arranged by size: 

                1046     707     252     173      79      41
 
SacI G_AGCT'C

Cuts at:      0    1639    2298 
   Size:       1639     659
 
SanDI GG'GwC_CC

Cuts at:      0     336    2298 
   Size:        336    1962
 
SapI GCTCTTCn'nnn_

Cuts at:      0     313    1627    2298 
   Size:        313    1314     671

  Fragments arranged by size: 

                1314     671     313
 
ScaI AGT'ACT

Cuts at:      0    1045    1729    2298 
   Size:       1045     684     569

  Fragments arranged by size: 

                1045     684     569
 
SexAI A'CCwGG_T

Cuts at:      0     893    2298 
   Size:        893    1405
 
SfcI C'TryA_G

Cuts at:      0     520    1183    1395    1564    1893    2065    2273    2298 
   Size:        520     663     212     169     329     172     208      25

  Fragments arranged by size: 

                 663     520     329     212     208     172     169      25
            
 
SfoI GGC'GCC

Cuts at:      0    1920    2298 
   Size:       1920     378
 
SmaI CCC'GGG

Cuts at:      0    1963    2298 
   Size:       1963     335
 
SrfI GCCC'GGGC

Cuts at:      0    1963    2298 
   Size:       1963     335
 
StyI C'CwwG_G

Cuts at:      0     290     881     986    1463    1955    2055    2298 
   Size:        290     591     105     477     492     100     243

  Fragments arranged by size: 

                 591     492     477     290     243     105     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     445    1570    2182    2298 
   Size:        445    1125     612     116

  Fragments arranged by size: 

                1125     612     445     116
 
TatI w'GTAC_w

Cuts at:      0    1043    1193    1229    1727    2298 
   Size:       1043     150      36     498     571

  Fragments arranged by size: 

                1043     571     498     150      36
 
Tth111I GACn'n_nGTC

Cuts at:      0     895    2298 
   Size:        895    1403
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     198     549    1017    1930    2013    2298 
   Size:        198     351     468     913      83     285

  Fragments arranged by size: 

                 913     468     351     285     198      83
 
XmaI C'CCGG_G

Cuts at:      0    1961    2298 
   Size:       1961     337
 
XmnI GAAnn'nnTTC

Cuts at:      0    1435    2298 
   Size:       1435     863
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FseI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PpiI
    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SapI     ScaI
    SexAI     SfcI     SfoI     SmaI     SrfI     StyI    TaqII     TatI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI     BclI
    BfrBI    BglII     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsiWI
    BsmBI    BsrDI   BssHII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     DrdI    EcoRI    EcoRV     FalI    FspAI     HpaI     KpnI     MfeI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SbfI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     SwaI     TstI     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com