Restriction map for
pFN21AA1462
##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AclI AflII Aor51HI
AscI AseI AsnI Asp718I BfrI
BsaBI BsiWI BsmBI BspDI BspHI
BsrDI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FseI HpaI KpnI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 174 GACGTC GACGT^C
AccIII 3308 TCCGGA T^CCGGA
AhdI 3139 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2825 GTGCAC G^TGCAC
ApaI 2421 GGGCCC GGGCC^C
ApaLI 2825 GTGCAC G^TGCAC
AspEI 3139 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3556 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 793 TGCGCA TGC^GCA
BamHI 3263 GGATCC G^GATCC
BbeI 86 GGCGCC GGCGC^C
BcgI 3342 CGATGCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1950 AGATCT A^GATCT
BseAI 3308 TCCGGA T^CCGGA
BsiEI 1127 CGACCG CG[GA][CT]^CG
Bsp1407I 2 TGTACA T^GTACA
BspEI 3308 TCCGGA T^CCGGA
BsrGI 2 TGTACA T^GTACA
BstBI 3601 TTCGAA TT^CGAA
DraI 1300 TTTAAA TTT^AAA
Eam1105I 3139 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1156 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 793 TGCGCA TGC^GCA
HaeII 86 GGCGCC [GA]GCGC^[CT]
HincII 1112 GTCAAC GT[CT]^[GA]AC
HindII 1112 GTCAAC GT[CT]^[GA]AC
HindIII 1339 AAGCTT A^AGCTT
KasI 86 GGCGCC G^GCGCC
MroI 3308 TCCGGA T^CCGGA
NaeI 1386 GCCGGC GCC^GGC
NarI 86 GGCGCC GG^CGCC
NcoI 457 CCATGG C^CATGG
NgoMIV 1386 GCCGGC G^CCGGC
NspV 3601 TTCGAA TT^CGAA
PflFI 3556 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 4014 GAAGAGC GCTCTTC(1/4)
SfoI 86 GGCGCC GGC^GCC
SfuI 3601 TTCGAA TT^CGAA
SspBI 2 TGTACA T^GTACA
TaqI 3602 TCGA T^CGA
Tth111I 3556 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3602 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 192 ACATGT A^C[GA][CT]GT
654 ACATGT
AocI 1210 CCTCAGG CC^T[ACGT]AGG
3508 CCTCAGG
Asp700I 2930 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3701 GAAGCCCTTC
AvrII 2986 CCTAGG C^CTAGG
3751 CCTAGG
BalI 2041 TGGCCA TGG^CCA
2766 TGGCCA
BbrPI 442 CACGTG CAC^GTG
2774 CACGTG
BbvCI 299 CCTCAGC CCTCAGC(-5/-2)
2265 CCTCAGC
BciVI 34 GGATAC GTATCC(6/5)
780 GTATCC
BglI 1209 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3496 GCCAGGCGGGC
BlnI 2986 CCTAGG C^CTAGG
3751 CCTAGG
BsaI 50 GAGACC GGTCTC(1/5)
1550 GAGACC
BspLU11I 192 ACATGT A^CATGT
654 ACATGT
BspMI 1651 ACCTGC ACCTGC(4/8)
2905 GCAGGT
BsrBI 920 GAGCGG CCGCTC(-3/-3)
3314 GAGCGG
BstYI 1950 AGATCT [GA]^GATC[CT]
3263 GGATCC
Bsu36I 1210 CCTCAGG CC^T[ACGT]AGG
3508 CCTCAGG
CfoI 87 GCGC GCG^C
794 GCGC
Eco81I 1210 CCTCAGG CC^T[ACGT]AGG
3508 CCTCAGG
EcoRI 663 GAATTC G^AATTC
819 GAATTC
HhaI 87 GCGC GCG^C
794 GCGC
HinP1I 87 GCGC G^CGC
794 GCGC
KspI 179 CCGCGG CCGC^GG
2643 CCGCGG
MflI 1950 AGATCT [GA]^GATC[CT]
3263 GGATCC
MluNI 2041 TGGCCA TGG^CCA
2766 TGGCCA
MscI 2041 TGGCCA TGG^CCA
2766 TGGCCA
PflMI 948 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3933 CCAGAGATTGG
PmaCI 442 CACGTG CAC^GTG
2774 CACGTG
PmlI 442 CACGTG CAC^GTG
2774 CACGTG
PpuMI 528 AGGTCCT [GA]G^G[AT]CC[CT]
2260 AGGTCCC
PvuII 2107 CAGCTG CAG^CTG
2978 CAGCTG
SacII 179 CCGCGG CCGC^GG
2643 CCGCGG
StuI 1464 AGGCCT AGG^CCT
3900 AGGCCT
Van91I 948 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3933 CCAGAGATTGG
XhoII 1950 AGATCT [GA]^GATC[CT]
3263 GGATCC
XmnI 2930 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3701 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AgeI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI Cac8I CelII Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI Hin1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PinAI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmaCI XmaI