(Circular) (Six-base) MAPSORT of: pFN21AA1291.seq Check: 4042 from: 1 to: 8317 pFN21AA1291 8317 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6127 278 Size: 53 83 186 936 4591 2468 Fragments arranged by size: 4591 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4179 5660 1937 Size: 2242 1481 4594 Fragments arranged by size: 4594 2242 1481 Acc65I G'GTAC_C Cuts at: 5325 5638 5325 Size: 313 8004 AclI AA'CG_TT Cuts at: 1563 6444 6817 1563 Size: 4881 373 3063 Fragments arranged by size: 4881 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 2294 2557 2777 2981 3124 6380 7764 Size: 560 102 263 220 204 143 3256 1384 Cuts at: 7764 8032 1632 Size: 268 1917 Fragments arranged by size: 3256 1917 1384 560 268 263 220 204 143 102 AfeI AGC'GCT Cuts at: 2087 5085 2087 Size: 2998 5319 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8120 AflIII A'CryG_T Cuts at: 1203 2221 7216 8193 1203 Size: 1018 4995 977 1327 Fragments arranged by size: 4995 1327 1018 977 AgeI A'CCGG_T Cuts at: 7148 7148 Size: 8317 AhdI GACnn_n'nnGTC Cuts at: 2716 7046 2716 Size: 4330 3987 AleI CACnn'nnGTG Cuts at: 1166 4962 5228 1166 Size: 3796 266 4255 Fragments arranged by size: 4255 3796 266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8317 AlwNI CAG_nnn'CTG Cuts at: 2215 3026 4041 4312 6060 7632 2215 Size: 811 1015 271 1748 1572 2900 Fragments arranged by size: 2900 1748 1572 1015 811 271 ApaLI G'TGCA_C Cuts at: 4158 6374 7530 4158 Size: 2216 1156 4945 Fragments arranged by size: 4945 2216 1156 ApoI r'AATT_y Cuts at: 1493 3442 3579 5626 5852 6004 8113 1493 Size: 1949 137 2047 226 152 2109 1697 Fragments arranged by size: 2109 2047 1949 1697 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 5090 6871 160 Size: 872 4058 1781 1606 Fragments arranged by size: 4058 1781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8317 AvaI C'yCGr_G Cuts at: 1870 1943 3321 3786 4902 5642 1870 Size: 73 1378 465 1116 740 4545 Fragments arranged by size: 4545 1378 1116 740 465 73 AvrII C'CTAG_G Cuts at: 4555 4555 Size: 8317 BamHI G'GATC_C Cuts at: 3364 3637 4138 5647 6014 3364 Size: 273 501 1509 367 5667 Fragments arranged by size: 5667 1509 501 367 273 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4024 5325 5638 7093 618 Size: 325 217 627 2237 1301 313 1455 1842 Fragments arranged by size: 2237 1842 1455 1301 627 325 313 217 BanII G_rGCy'C Cuts at: 1391 3136 5636 1391 Size: 1745 2500 4072 Fragments arranged by size: 4072 2500 1745 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2633 2807 2856 3481 3715 4480 928 Size: 971 734 174 49 625 234 765 4765 Fragments arranged by size: 4765 971 765 734 625 234 174 49 BbvCI CC'TCA_GC Cuts at: 5020 5020 Size: 8317 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6508 6542 1903 Size: 34 4571 34 3678 Fragments arranged by size: 4571 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4750 6209 7419 4750 Size: 1459 1210 5648 Fragments arranged by size: 5648 1459 1210 BclI T'GATC_A Cuts at: 1548 2373 1548 Size: 825 7492 BfrBI ATG'CAT Cuts at: 2893 2893 Size: 8317 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4388 6928 136 Size: 107 122 71 631 605 2716 2540 1525 Fragments arranged by size: 2716 2540 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8312 8312 Size: 8317 BlpI GC'TnA_GC Cuts at: 4627 5734 4627 Size: 1107 7210 Bme1580I G_kGCm'C Cuts at: 1790 3946 4162 6378 7534 1790 Size: 2156 216 2216 1156 2573 Fragments arranged by size: 2573 2216 2156 1156 216 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8317 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2722 6048 7006 Size: 416 53 83 399 24 1305 3326 958 Cuts at: 7006 442 Size: 1753 Fragments arranged by size: 3326 1753 1305 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4382 1056 Size: 3326 4991 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4803 4803 Size: 8317 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2777 3486 4154 4895 5351 5582 6977 1239 Size: 1538 709 668 741 456 231 1395 2579 Fragments arranged by size: 2579 1538 1395 741 709 668 456 231 Bpu10I CC'TnA_GC Cuts at: 5020 5020 Size: 8317 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 2179 4035 4848 6442 7307 7605 2090 Size: 89 1856 813 1594 865 298 2802 Fragments arranged by size: 2802 1856 1594 865 813 298 89 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6980 882 Size: 617 5481 2219 Fragments arranged by size: 5481 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5214 6108 493 Size: 711 4010 894 2702 Fragments arranged by size: 4010 2702 894 711 BsaBI GATnn'nnATC Cuts at: 2468 8311 2468 Size: 5843 2474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6124 6506 7141 275 Size: 53 83 186 936 4591 382 635 1451 Fragments arranged by size: 4591 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2853 6750 7148 7422 7569 7980 2853 Size: 3897 398 274 147 411 3190 Fragments arranged by size: 3897 3190 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 2252 2282 2750 Size: 30 165 30 657 30 162 30 468 Cuts at: 2750 2780 2922 2952 4127 4157 1178 Size: 30 142 30 1175 30 5338 Fragments arranged by size: 5338 1175 657 468 165 162 142 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2342 2943 3307 3820 3893 4028 4892 Size: 946 210 601 364 513 73 135 864 Cuts at: 4892 5159 1186 Size: 267 4344 Fragments arranged by size: 4344 946 864 601 513 364 267 210 135 73 BseYI C'CCAG_C Cuts at: 2082 2989 3013 3389 3852 4241 4614 4972 7520 Size: 907 24 376 463 389 373 358 2548 Cuts at: 7520 8016 2082 Size: 496 2383 Fragments arranged by size: 2548 2383 907 496 463 389 376 373 358 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3039 3527 3660 4282 1163 Size: 573 1303 488 133 622 5198 Fragments arranged by size: 5198 1303 622 573 488 133 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5767 6528 7556 1540 Size: 275 186 3766 761 1028 2301 Fragments arranged by size: 3766 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3328 4162 4707 4725 4969 6378 6463 7534 3328 Size: 834 545 18 244 1409 85 1071 4111 Fragments arranged by size: 4111 1409 1071 834 545 244 85 18 BsmI GAATG_Cn' Cuts at: 4185 4515 5828 5921 4185 Size: 330 1313 93 6581 Fragments arranged by size: 6581 1313 330 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4977 5558 4977 Size: 581 7736 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3136 3328 3946 4162 4707 4725 4969 Size: 399 1346 192 618 216 545 18 244 Cuts at: 4969 5636 6378 6463 7534 1391 Size: 667 742 85 1071 2174 Fragments arranged by size: 2174 1346 1071 742 667 618 545 399 244 216 192 85 18 BspEI T'CCGG_A Cuts at: 7980 7980 Size: 8317 BspHI T'CATG_A Cuts at: 2061 6206 7936 2061 Size: 4145 1730 2442 Fragments arranged by size: 4145 2442 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5672 844 Size: 4828 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6204 1394 Size: 429 4381 3507 Fragments arranged by size: 4381 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2820 3008 5463 6812 6986 65 Size: 1183 1572 188 2455 1349 174 1396 Fragments arranged by size: 2455 1572 1396 1349 1183 188 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 6961 7148 1700 Size: 110 281 4870 187 2869 Fragments arranged by size: 4870 2869 281 187 110 BsrGI T'GTAC_A Cuts at: 96 5078 96 Size: 4982 3335 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8317 BssSI C'ACGA_G Cuts at: 6377 7389 6377 Size: 1012 7305 BstAPI GCAn_nnn'nTGC Cuts at: 794 2918 4066 794 Size: 2124 1148 5045 Fragments arranged by size: 5045 2124 1148 BstBI TT'CG_AA Cuts at: 3559 7121 3559 Size: 3562 4755 BstEII G'GTnAC_C Cuts at: 1184 5011 1184 Size: 3827 4490 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2397 3389 4411 5329 2011 Size: 386 992 1022 918 4999 Fragments arranged by size: 4999 1022 992 918 386 BstYI r'GATC_y Cuts at: 1972 2211 2568 3364 3637 4138 5647 6014 6399 Size: 239 357 796 273 501 1509 367 385 Cuts at: 6399 6416 7868 7954 7966 8312 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1509 1452 796 501 385 367 357 346 273 239 86 17 12 BstZ17I GTA'TAC Cuts at: 4180 4180 Size: 8317 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6826 Fragments arranged by size: 6826 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2024 3303 487 Size: 683 56 798 1279 5501 Fragments arranged by size: 5501 1279 798 683 56 BtsI GCAGTG_nn' Cuts at: 2733 3515 3798 5192 5312 5833 6627 6647 2733 Size: 782 283 1394 120 521 794 20 4403 Fragments arranged by size: 4403 1394 794 782 521 283 120 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8317 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5590 5625 529 Size: 35 5026 35 3221 Fragments arranged by size: 5026 3221 35 35 DraI TTT'AAA Cuts at: 2125 5406 5621 2125 Size: 3281 215 4821 Fragments arranged by size: 4821 3281 215 DraIII CAC_nnn'GTG Cuts at: 4066 4066 Size: 8317 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4484 7324 809 Size: 507 3168 2840 1802 Fragments arranged by size: 3168 2840 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2387 4032 5001 Size: 54 1425 50 275 18 557 1645 969 Cuts at: 5001 5598 5764 6653 8282 8 Size: 597 166 889 1629 43 Fragments arranged by size: 1645 1629 1425 969 889 597 557 275 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5764 1537 Size: 275 3952 4090 Fragments arranged by size: 4090 3952 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 2957 3115 3143 4109 4224 4712 6247 Size: 966 638 158 28 966 115 488 1535 Cuts at: 6247 1353 Size: 3423 Fragments arranged by size: 3423 1535 966 966 638 488 158 115 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2896 6904 7278 7424 387 Size: 1064 138 1307 4008 374 146 1280 Fragments arranged by size: 4008 1307 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2294 2557 2777 2981 3124 3486 Size: 393 560 102 263 220 204 143 362 Cuts at: 3486 4154 4895 5351 5582 6380 6977 7764 8032 Size: 668 741 456 231 798 597 787 268 Cuts at: 8032 1239 Size: 1524 Fragments arranged by size: 1524 798 787 741 668 597 560 456 393 362 268 263 231 220 204 143 102 EcoNI CCTnn'n_nnAGG Cuts at: 3716 4229 4559 5137 3716 Size: 513 330 578 6896 Fragments arranged by size: 6896 578 513 330 EcoO109I rG'GnC_Cy Cuts at: 3775 4568 4603 3775 Size: 793 35 7489 Fragments arranged by size: 7489 793 35 EcoRI G'AATT_C Cuts at: 3442 5626 6004 3442 Size: 2184 378 5755 Fragments arranged by size: 5755 2184 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3247 3247 Size: 8317 FspI TGC'GCA Cuts at: 6823 6823 Size: 8317 HaeII r_GCGC'y Cuts at: 1678 2089 5087 5440 7464 1678 Size: 411 2998 353 2024 2531 Fragments arranged by size: 2998 2531 2024 411 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2336 2368 2702 Size: 32 135 32 488 32 244 32 334 Cuts at: 2702 2734 2956 2988 5114 5146 6958 6990 7032 Size: 32 222 32 2126 32 1812 32 42 Cuts at: 7032 7064 1373 Size: 32 2626 Fragments arranged by size: 2626 2126 1812 488 334 244 222 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4639 4899 5661 5907 669 Size: 988 281 2701 260 762 246 3079 Fragments arranged by size: 3079 2701 988 762 281 260 246 HindIII A'AGCT_T Cuts at: 748 5399 748 Size: 4651 3666 HpaI GTT'AAC Cuts at: 4899 5907 4899 Size: 1008 7309 KpnI G_GTAC'C Cuts at: 5329 5642 5329 Size: 313 8004 MfeI C'AATT_G Cuts at: 5916 5916 Size: 8317 MluI A'CGCG_T Cuts at: 8193 8193 Size: 8317 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2826 3439 7431 7615 1305 Size: 1521 613 3992 184 2007 Fragments arranged by size: 3992 2007 1521 613 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2389 4034 5003 5600 10 Size: 54 1425 343 557 1645 969 597 2727 Fragments arranged by size: 2727 1645 1425 969 597 557 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3551 3833 4962 5070 5228 6275 Size: 648 843 1542 282 1129 108 158 1047 Cuts at: 6275 6634 6793 518 Size: 359 159 2042 Fragments arranged by size: 2042 1542 1129 1047 843 648 359 282 159 158 108 MspA1I CmG'CkG Cuts at: 1606 2041 2807 3001 5733 6410 7558 7803 1606 Size: 435 766 194 2732 677 1148 245 2120 Fragments arranged by size: 2732 2120 1148 766 677 435 245 194 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6826 Fragments arranged by size: 6826 939 552 NdeI CA'TA_TG Cuts at: 387 4776 387 Size: 4389 3928 NheI G'CTAG_C Cuts at: 1052 4378 1052 Size: 3326 4991 NotI GC'GGCC_GC Cuts at: 5764 5764 Size: 8317 NsiI A_TGCA'T Cuts at: 2895 2895 Size: 8317 NspI r_CATG'y Cuts at: 1132 1229 2251 2410 3357 3815 3880 4060 5675 Size: 97 1022 159 947 458 65 180 1615 Cuts at: 5675 7220 1132 Size: 1545 2229 Fragments arranged by size: 2229 1615 1545 1022 947 458 180 159 97 65 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8317 PciI A'CATG_T Cuts at: 7216 7216 Size: 8317 PflMI CCAn_nnn'nTGG Cuts at: 1108 2560 4041 4979 1108 Size: 1452 1481 938 4446 Fragments arranged by size: 4446 1481 1452 938 PfoI T'CCnGG_A Cuts at: 2214 2754 2214 Size: 540 7777 PmeI GTTT'AAAC Cuts at: 5621 5621 Size: 8317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6386 6418 7899 7931 6386 Size: 32 1481 32 6772 Fragments arranged by size: 6772 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4603 4603 Size: 8317 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8317 PsiI TTA'TAA Cuts at: 3885 4343 5887 3885 Size: 458 1544 6315 Fragments arranged by size: 6315 1544 458 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4339 4371 4339 Size: 32 8285 PstI C_TGCA'G Cuts at: 830 3457 4846 5421 5669 830 Size: 2627 1389 575 248 3478 Fragments arranged by size: 3478 2627 1389 575 248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8317 PvuII CAG'CTG Cuts at: 2041 3001 2041 Size: 960 7357 SalI G'TCGA_C Cuts at: 1936 5659 1936 Size: 3723 4594 SapI GCTCTTCn'nnn_ Cuts at: 3115 4109 4712 3115 Size: 994 603 6720 Fragments arranged by size: 6720 994 603 SbfI CC_TGCA'GG Cuts at: 5669 5669 Size: 8317 ScaI AGT'ACT Cuts at: 6565 8277 6565 Size: 1712 6605 SfcI C'TryA_G Cuts at: 826 1045 3453 3697 4842 5417 5665 6800 7481 Size: 219 2408 244 1145 575 248 1135 681 Cuts at: 7481 7672 826 Size: 191 1471 Fragments arranged by size: 2408 1471 1145 1135 681 575 248 244 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8317 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8317 SmaI CCC'GGG Cuts at: 1872 3788 5644 1872 Size: 1916 1856 4545 Fragments arranged by size: 4545 1916 1856 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 2158 4050 4863 6421 7322 Size: 197 926 162 53 1892 813 1558 901 Cuts at: 7322 7584 820 Size: 262 1553 Fragments arranged by size: 1892 1558 1553 926 901 813 262 197 162 53 SnaBI TAC'GTA Cuts at: 493 6108 493 Size: 5615 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8317 SphI G_CATG'C Cuts at: 1132 3815 5675 1132 Size: 2683 1860 3774 Fragments arranged by size: 3774 2683 1860 SspI AAT'ATT Cuts at: 5 52 3266 4651 6241 5 Size: 47 3214 1385 1590 2081 Fragments arranged by size: 3214 2081 1590 1385 47 StuI AGG'CCT Cuts at: 1517 4131 1517 Size: 2614 5703 StyI C'CwwG_G Cuts at: 513 1065 2004 3633 4404 4555 5487 5756 513 Size: 552 939 1629 771 151 932 269 3074 Fragments arranged by size: 3074 1629 939 932 771 552 269 151 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2096 2704 3005 3563 4181 6344 6361 Size: 1255 516 608 301 558 618 2163 17 Cuts at: 6361 6514 6699 7144 325 Size: 153 185 445 1498 Fragments arranged by size: 2163 1498 1255 618 608 558 516 445 301 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5078 6563 8275 Size: 274 80 33 51 1443 3101 1485 1712 Cuts at: 8275 96 Size: 138 Fragments arranged by size: 3101 1712 1485 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2750 2782 3073 3105 4127 4159 4239 4271 8277 Size: 32 291 32 1022 32 80 32 4006 Cuts at: 8277 8309 2750 Size: 32 2758 Fragments arranged by size: 4006 2758 1022 291 80 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8317 XbaI T'CTAG_A Cuts at: 5653 5653 Size: 8317 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3214 3214 Size: 8317 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8317 XmaI C'CCGG_G Cuts at: 1870 3786 5642 1870 Size: 1916 1856 4545 Fragments arranged by size: 4545 1916 1856 XmnI GAAnn'nnTTC Cuts at: 3069 3579 4004 6446 3069 Size: 510 425 2442 4940 Fragments arranged by size: 4940 2442 510 425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6125 276 Size: 53 83 186 936 4591 2468 Fragments arranged by size: 4591 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BsiWI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI