Restriction map for
pFN21AA1097
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspHI AspI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BciVI BcnI BfrI BglI BlnI
BmyI BsaAI BsaHI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AseI 1021 ATTAAT AT^TAAT
AsnI 1021 ATTAAT AT^TAAT
Asp700I 727 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1402 CTCGAG C^[CT]CG[GA]G
BanI 1257 GGCACC G^G[CT][GA]CC
BcgI 1509 CGAAGCATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1977 TGATCA T^GATCA
BglII 1416 AGATCT A^GATCT
BlpI 1453 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1453 GCTAAGC GC^T[ACGT]AGC
BsaBI 2503 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1405 GAGACC GGTCTC(1/5)
BsaWI 2664 TCCGGT [AT]^CCGG[AT]
BsgI 1366 GTGCAG GTGCAG(16/14)
BsmFI 1579 GTCCC GGGAC(10/14)
BsoBI 1402 CTCGAG C^[CT]CG[GA]G
BspHI 756 TCATGA T^CATGA
BsrDI 302 CATTGC GCAATG(2/0)
CelII 1453 GCTAAGC GC^T[ACGT]AGC
DraI 355 TTTAAA TTT^AAA
EcoNI 1228 CCTAAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 727 GAATTC G^AATTC
EcoRV 2082 GATATC GAT^ATC
FbaI 1977 TGATCA T^GATCA
HapII 2665 CCGG C^CGG
HindIII 2024 AAGCTT A^AGCTT
HpaII 2665 CCGG C^CGG
MamI 2503 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2312 CAGCTG C[AC]G^C[GT]G
MspI 2665 CCGG C^CGG
NspI 124 GCATGT [GA]CATG^[CT]
PaeR7I 1402 CTCGAG C^TCGAG
PflMI 1429 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2517 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1021 ATTAAT AT^TAAT
PstI 2620 CTGCAG CTGCA^G
PvuII 2312 CAGCTG CAG^CTG
RcaI 756 TCATGA T^CATGA
SalI 1081 GTCGAC G^TCGAC
SapI 2046 GAAGAGC GCTCTTC(1/4)
SexAI 2249 ACCTGGT A^CC[AT]GGT
SspI 472 AATATT AAT^ATT
Van91I 1429 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 913 TCTAGA T^CTAGA
XhoI 1402 CTCGAG C^TCGAG
XmnI 727 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 1081 GTCGAC GT^[AC][GT]AC
1167 GTCTAC
AhdI 1384 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2309 GACCAGCTGTC
AspEI 1384 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2309 GACCAGCTGTC
BalI 1246 TGGCCA TGG^CCA
2589 TGGCCA
BsaJI 78 CCTTGG C^C[ACGT][ACGT]GG
105 CCAAGG
BsmI 495 GAATGC GAATGC(1/-1)
1461 GCATTC
BspMI 582 ACCTGC ACCTGC(4/8)
2294 GCAGGT
BstBI 11 TTCGAA TT^CGAA
1155 TTCGAA
BstXI 112 CCAAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1522 CCACAAGGGTGG
DraII 2517 AGGTCCT [GA]G^G[ACGT]CC[CT]
2640 AGGGCCT
EaeI 1246 TGGCCA [CT]^GGCC[GA]
2589 TGGCCA
Eam1105I 1384 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2309 GACCAGCTGTC
EcoO109I 2517 AGGTCCT [GA]G^G[ACGT]CC[CT]
2640 AGGGCCT
EcoT14I 78 CCTTGG C^C[AT][AT]GG
105 CCAAGG
HincII 1081 GTCGAC GT[CT]^[GA]AC
2317 GTCAAC
HindII 1081 GTCGAC GT[CT]^[GA]AC
2317 GTCAAC
MaeII 1209 ACGT A^CGT
2030 ACGT
MluNI 1246 TGGCCA TGG^CCA
2589 TGGCCA
MscI 1246 TGGCCA TGG^CCA
2589 TGGCCA
NspV 11 TTCGAA TT^CGAA
1155 TTCGAA
PleI 1063 GACTC GAGTC(4/5)
2431 GACTC
SfuI 11 TTCGAA TT^CGAA
1155 TTCGAA
SmlI 722 CTCAAG C^T[CT][GA]AG
1402 CTCGAG
StyI 78 CCTTGG C^C[AT][AT]GG
105 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbsI BbvI
BfaI BpmI BpuAI BseRI BsiYI
BslI BsmAI Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII