Restriction map for
pFN21AA1033
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccIII AclI AcyI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BglI BlnI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstPI
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FauI FseI FspI HaeII
Hin1I HpaI KasI KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 69 enzymes) #
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Acc65I 1038 GGTACC G^GTACC
AflII 82 CTTAAG C^TTAAG
AhdI 3335 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2707 CCTGAGG CC^T[ACGT]AGG
AseI 2452 ATTAAT AT^TAAT
AsnI 2452 ATTAAT AT^TAAT
Asp700I 1726 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1038 GGTACC G^GTACC
AspEI 3335 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 12 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1553 TGGCCA TGG^CCA
BanI 1038 GGTACC G^G[CT][GA]CC
BanII 1227 GAGCTC G[GA]GC[CT]^C
BbvCI 853 GCTGAGG CCTCAGC(-5/-2)
BcnI 21 CCCGG CC^[GC]GG
BfrI 82 CTTAAG C^TTAAG
BlpI 3262 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3262 GCTAAGC GC^T[ACGT]AGC
BsaAI 2182 TACGTA [CT]AC^GT[GA]
BsmI 3078 GCATTC GAATGC(1/-1)
BspMI 1923 ACCTGC ACCTGC(4/8)
Bst1107I 111 GTATAC GTA^TAC
BstZ17I 111 GTATAC GTA^TAC
Bsu36I 2707 CCTGAGG CC^T[ACGT]AGG
CelII 3262 GCTAAGC GC^T[ACGT]AGC
CfoI 1911 GCGC GCG^C
DraII 2333 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 3152 CCATGG C^C[GA][CT]GG
EaeI 1553 TGGCCA [CT]^GGCC[GA]
Eam1105I 3335 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1535 CTCTTC CTCTTC(1/4)
Eco81I 2707 CCTGAGG CC^T[ACGT]AGG
EcoO109I 2333 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 704 GAATTC G^AATTC
EcoT14I 3152 CCATGG C^C[AT][AT]GG
HapII 22 CCGG C^CGG
HhaI 1911 GCGC GCG^C
HinP1I 1911 GCGC G^CGC
HincII 43 GTTGAC GT[CT]^[GA]AC
HindII 43 GTTGAC GT[CT]^[GA]AC
HpaII 22 CCGG C^CGG
KpnI 1038 GGTACC GGTAC^C
Ksp632I 1535 CTCTTC CTCTTC(1/4)
MfeI 155 CAATTG C^AATTG
MluNI 1553 TGGCCA TGG^CCA
MscI 1553 TGGCCA TGG^CCA
MspA1I 1109 CAGCTG C[AC]G^C[GT]G
MspI 22 CCGG C^CGG
MunI 155 CAATTG C^AATTG
NciI 21 CCCGG CC^[GC]GG
NcoI 3152 CCATGG C^CATGG
NdeI 1462 CATATG CA^TATG
PflFI 12 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 486 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2452 ATTAAT AT^TAAT
PvuII 1109 CAGCTG CAG^CTG
SacI 1227 GAGCTC GAGCT^C
SapI 1534 GCTCTTC GCTCTTC(1/4)
ScaI 844 AGTACT AGT^ACT
SnaBI 2182 TACGTA TAC^GTA
SpeI 2865 ACTAGT A^CTAGT
SphI 1935 GCATGC GCATG^C
StuI 785 AGGCCT AGG^CCT
StyI 3152 CCATGG C^C[AT][AT]GG
Tsp45I 1802 GTGAC ^GT[GC]AC
Tth111I 12 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 486 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2315 CCAGTCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1726 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 111 GTATAC GT^[AC][GT]AC
2983 GTAGAC
AccII 40 CGCG CG^CG
1912 CGCG
AflIII 1382 ACATGT A^C[GA][CT]GT
1895 ACATGT
AlwNI 433 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1106 CAGCAGCTG
AvaII 1645 GGACC G^G[AT]CC
2631 GGACC
BciVI 1161 GGATAC GTATCC(6/5)
2649 GTATCC
BpmI 2996 CTCCAG CTGGAG(16/14)
545 CTGGAG
BsaI 3476 GAGACC GGTCTC(1/5)
343 GGTCTC
BseRI 106 GAGGAG GAGGAG(10/8)
821 GAGGAG
BsgI 284 CTGCAC GTGCAG(16/14)
1428 GTGCAG
BspHI 1748 TCATGA T^CATGA
2235 TCATGA
BspLU11I 1382 ACATGT A^CATGT
1895 ACATGT
BsrDI 2813 GCAATG GCAATG(2/0)
3470 GCAATG
BstUI 40 CGCG CG^CG
1912 CGCG
BstXI 377 CCAGCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3152 CCATGGGTGTGG
DraIII 1685 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1796 CACAATGTG
EcoT22I 1337 ATGCAT ATGCA^T
1933 ATGCAT
HgaI 139 GACGC GACGC(5/10)
1596 GCGTC
HindIII 199 AAGCTT A^AGCTT
3175 AAGCTT
MvnI 40 CGCG CG^CG
1912 CGCG
NlaIV 1038 GGTACC GG[ACGT]^[ACGT]CC
3215 GGTTCC
NsiI 1337 ATGCAT ATGCA^T
1933 ATGCAT
RcaI 1748 TCATGA T^CATGA
2235 TCATGA
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BbsI BbvI BclI
BfaI BglII BmyI BpuAI BsaBI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstBI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EcoRII FbaI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NspI NspV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII