(Circular) (Six-base) MAPSORT of: pFN21AA1033.seq Check: 9365 from: 1 to: 8224 pFN21AA1033 8224 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6034 278 Size: 53 83 186 936 4498 2468 Fragments arranged by size: 4498 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2117 4989 5567 1937 Size: 180 2872 578 4594 Fragments arranged by size: 4594 2872 578 180 Acc65I G'GTAC_C Cuts at: 3043 5545 3043 Size: 2502 5722 AclI AA'CG_TT Cuts at: 1563 6351 6724 1563 Size: 4788 373 3063 Fragments arranged by size: 4788 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2771 3315 5267 5389 5448 6287 7671 7939 Size: 1139 544 1952 122 59 839 1384 268 Cuts at: 7939 1632 Size: 1917 Fragments arranged by size: 1952 1917 1384 1139 839 544 268 122 59 AflII C'TTAA_G Cuts at: 820 1017 2087 820 Size: 197 1070 6957 Fragments arranged by size: 6957 1070 197 AflIII A'CryG_T Cuts at: 1203 3387 3900 7123 8100 1203 Size: 2184 513 3223 977 1327 Fragments arranged by size: 3223 2184 1327 977 513 AgeI A'CCGG_T Cuts at: 7055 7055 Size: 8224 AhdI GACnn_n'nnGTC Cuts at: 5345 6953 5345 Size: 1608 6616 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8224 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8224 AlwNI CAG_nnn'CTG Cuts at: 2443 3116 5967 7539 2443 Size: 673 2851 1572 3128 Fragments arranged by size: 3128 2851 1572 673 ApaLI G'TGCA_C Cuts at: 6281 7437 6281 Size: 1156 7068 ApoI r'AATT_y Cuts at: 1493 2067 2102 2336 2406 2702 2709 2864 2955 Size: 574 35 234 70 296 7 155 91 Cuts at: 2955 3287 4002 4676 4858 4951 5006 5038 5360 Size: 332 715 674 182 93 55 32 322 Cuts at: 5360 5397 5533 5759 5911 8020 1493 Size: 37 136 226 152 2109 1697 Fragments arranged by size: 2109 1697 715 674 574 332 322 296 234 226 182 155 152 136 93 91 70 55 37 35 32 7 AseI AT'TA_AT Cuts at: 160 1032 4458 6778 160 Size: 872 3426 2320 1606 Fragments arranged by size: 3426 2320 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8224 AvaI C'yCGr_G Cuts at: 1870 1943 5549 1870 Size: 73 3606 4545 Fragments arranged by size: 4545 3606 73 BamHI G'GATC_C Cuts at: 5554 5921 5554 Size: 367 7857 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3043 5545 7000 618 Size: 325 217 627 1256 2502 1455 1842 Fragments arranged by size: 2502 1842 1455 1256 627 325 217 BanII G_rGCy'C Cuts at: 1391 3236 5543 1391 Size: 1845 2307 4072 Fragments arranged by size: 4072 2307 1845 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3134 3832 4796 928 Size: 971 1235 698 964 4356 Fragments arranged by size: 4356 1235 971 964 698 BbvCI CC'TCA_GC Cuts at: 2859 2859 Size: 8224 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6415 6449 1903 Size: 34 4478 34 3678 Fragments arranged by size: 4478 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3160 4665 6116 7326 3160 Size: 1505 1451 1210 4058 Fragments arranged by size: 4058 1505 1451 1210 BclI T'GATC_A Cuts at: 1548 4645 4732 4939 1548 Size: 3097 87 207 4833 Fragments arranged by size: 4833 3097 207 87 BfrBI ATG'CAT Cuts at: 3344 3940 3344 Size: 596 7628 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6835 136 Size: 107 122 71 631 605 5163 1525 Fragments arranged by size: 5163 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3298 4051 4793 8219 3298 Size: 753 742 3426 3303 Fragments arranged by size: 3426 3303 753 742 BlpI GC'TnA_GC Cuts at: 5268 5641 5268 Size: 373 7851 Bme1580I G_kGCm'C Cuts at: 1790 6285 7441 1790 Size: 4495 1156 2573 Fragments arranged by size: 4495 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8224 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 4255 4314 Size: 416 53 83 399 24 624 2214 59 Cuts at: 4314 5955 6913 442 Size: 1641 958 1753 Fragments arranged by size: 2214 1753 1641 958 624 416 399 83 59 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8224 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2137 2137 Size: 8224 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2571 4986 6884 1239 Size: 1332 2415 1898 2579 Fragments arranged by size: 2579 2415 1898 1332 Bpu10I CC'TnA_GC Cuts at: 2859 2859 Size: 8224 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2129 3437 6349 7214 7512 2129 Size: 1308 2912 865 298 2841 Fragments arranged by size: 2912 2841 1308 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2354 5475 6887 882 Size: 617 855 3121 1412 2219 Fragments arranged by size: 3121 2219 1412 855 617 BsaAI yAC'GTr Cuts at: 493 1204 4189 6015 493 Size: 711 2985 1826 2702 Fragments arranged by size: 2985 2702 1826 711 BsaBI GATnn'nnATC Cuts at: 2181 5193 5349 8218 2181 Size: 3012 156 2869 2187 Fragments arranged by size: 3012 2869 2187 156 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6031 6413 7048 275 Size: 53 83 186 936 4498 382 635 1451 Fragments arranged by size: 4498 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6657 7055 7329 7476 7887 6657 Size: 398 274 147 411 6994 Fragments arranged by size: 6994 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2006 2036 2820 2850 1178 Size: 30 165 30 603 30 784 30 6552 Fragments arranged by size: 6552 784 603 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2841 1186 Size: 940 715 6569 Fragments arranged by size: 6569 940 715 BseYI C'CCAG_C Cuts at: 7427 7923 7427 Size: 496 7728 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2274 3454 1163 Size: 573 538 1180 5933 Fragments arranged by size: 5933 1180 573 538 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5674 6435 7463 1540 Size: 275 186 3673 761 1028 2301 Fragments arranged by size: 3673 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2580 3236 3615 6285 6370 7441 2580 Size: 656 379 2670 85 1071 3363 Fragments arranged by size: 3363 2670 1071 656 379 85 BsmI GAATG_Cn' Cuts at: 5083 5735 5828 5083 Size: 652 93 7479 Fragments arranged by size: 7479 652 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2580 3236 3615 5543 6285 6370 7441 Size: 399 790 656 379 1928 742 85 1071 Cuts at: 7441 1391 Size: 2174 Fragments arranged by size: 2174 1928 1071 790 742 656 399 379 85 BspEI T'CCGG_A Cuts at: 7887 7887 Size: 8224 BspHI T'CATG_A Cuts at: 3753 4240 6113 7843 3753 Size: 487 1873 1730 4134 Fragments arranged by size: 4134 1873 1730 487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3937 5579 844 Size: 3093 1642 3489 Fragments arranged by size: 3489 3093 1642 BsrBI CCG'CTC Cuts at: 1394 1823 6111 1394 Size: 429 4288 3507 Fragments arranged by size: 4288 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4825 5482 6719 6893 65 Size: 1183 3577 657 1237 174 1396 Fragments arranged by size: 3577 1396 1237 1183 657 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6868 7055 1700 Size: 110 5058 187 2869 Fragments arranged by size: 5058 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2661 3208 5281 96 Size: 2565 547 2073 3039 Fragments arranged by size: 3039 2565 2073 547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8224 BssSI C'ACGA_G Cuts at: 6284 7296 6284 Size: 1012 7212 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8224 BstBI TT'CG_AA Cuts at: 2992 4486 5011 7028 2992 Size: 1494 525 2017 4188 Fragments arranged by size: 4188 2017 1494 525 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8224 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2389 5164 2011 Size: 378 2775 5071 Fragments arranged by size: 5071 2775 378 BstYI r'GATC_y Cuts at: 1972 3298 3766 4051 4793 5554 5921 6306 6323 Size: 1326 468 285 742 761 367 385 17 Cuts at: 6323 7775 7861 7873 8219 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1326 761 742 468 385 367 346 285 86 17 12 BstZ17I GTA'TAC Cuts at: 2118 2118 Size: 8224 Bsu36I CC'TnA_GG Cuts at: 4713 4713 Size: 8224 BtgI C'CryG_G Cuts at: 513 1065 2004 5157 513 Size: 552 939 3153 3580 Fragments arranged by size: 3580 3153 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7485 Fragments arranged by size: 7485 683 56 BtsI GCAGTG_nn' Cuts at: 5395 5740 6534 6554 5395 Size: 345 794 20 7065 Fragments arranged by size: 7065 794 345 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8224 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8189 DraI TTT'AAA Cuts at: 3721 4008 4080 5528 3721 Size: 287 72 1448 6417 Fragments arranged by size: 6417 1448 287 72 DraIII CAC_nnn'GTG Cuts at: 3695 3806 3695 Size: 111 8113 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7231 809 Size: 507 5915 1802 Fragments arranged by size: 5915 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3558 5671 6560 Size: 54 1425 50 275 18 1728 2113 889 Cuts at: 6560 8189 8 Size: 1629 43 Fragments arranged by size: 2113 1728 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5671 1537 Size: 275 3859 4090 Fragments arranged by size: 4090 3859 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3546 6154 1353 Size: 2193 2608 3423 Fragments arranged by size: 3423 2608 2193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6811 7185 7331 387 Size: 1064 138 5222 374 146 1280 Fragments arranged by size: 5222 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3234 3234 Size: 8224 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2571 2771 3315 4986 5267 5389 5448 Size: 393 939 200 544 1671 281 122 59 Cuts at: 5448 6287 6884 7671 7939 1239 Size: 839 597 787 268 1524 Fragments arranged by size: 1671 1524 939 839 787 597 544 393 281 268 200 122 59 EcoO109I rG'GnC_Cy Cuts at: 4339 4339 Size: 8224 EcoRI G'AATT_C Cuts at: 2709 5533 5911 2709 Size: 2824 378 5022 Fragments arranged by size: 5022 2824 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2102 2927 4904 2102 Size: 825 1977 5422 Fragments arranged by size: 5422 1977 825 FspI TGC'GCA Cuts at: 6730 6730 Size: 8224 HaeII r_GCGC'y Cuts at: 1678 7371 1678 Size: 5693 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2042 2074 3197 Size: 32 135 32 434 32 4 32 1123 Cuts at: 3197 3229 3599 3631 3777 3809 5331 5363 6865 Size: 32 370 32 146 32 1522 32 1502 Cuts at: 6865 6897 6939 6971 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1522 1502 1123 434 370 146 135 42 32 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 2050 5568 5814 669 Size: 988 281 112 3518 246 3079 Fragments arranged by size: 3518 3079 988 281 246 112 HindIII A'AGCT_T Cuts at: 748 2204 5180 748 Size: 1456 2976 3792 Fragments arranged by size: 3792 2976 1456 HpaI GTT'AAC Cuts at: 5814 5814 Size: 8224 KpnI G_GTAC'C Cuts at: 3047 5549 3047 Size: 2502 5722 MfeI C'AATT_G Cuts at: 2160 5823 2160 Size: 3663 4561 MluI A'CGCG_T Cuts at: 8100 8100 Size: 8224 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2107 2932 7338 7522 1305 Size: 802 825 4406 184 2007 Fragments arranged by size: 4406 2007 825 802 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3560 10 Size: 54 1425 343 1728 4674 Fragments arranged by size: 4674 1728 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3392 3491 3707 5162 6182 6541 Size: 648 843 1383 99 216 1455 1020 359 Cuts at: 6541 6700 518 Size: 159 2042 Fragments arranged by size: 2042 1455 1383 1020 843 648 359 216 159 99 MspA1I CmG'CkG Cuts at: 1606 3116 5640 6317 7465 7710 1606 Size: 1510 2524 677 1148 245 2120 Fragments arranged by size: 2524 2120 1510 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 5157 513 Size: 552 939 3153 3580 Fragments arranged by size: 3580 3153 939 552 NdeI CA'TA_TG Cuts at: 387 3468 387 Size: 3081 5143 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8224 NotI GC'GGCC_GC Cuts at: 5671 5671 Size: 8224 NsiI A_TGCA'T Cuts at: 3346 3942 3346 Size: 596 7628 NspI r_CATG'y Cuts at: 1132 1229 3391 3904 3944 5582 7127 1132 Size: 97 2162 513 40 1638 1545 2229 Fragments arranged by size: 2229 2162 1638 1545 513 97 40 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8224 PciI A'CATG_T Cuts at: 3387 3900 7123 3387 Size: 513 3223 4488 Fragments arranged by size: 4488 3223 513 PflMI CCAn_nnn'nTGG Cuts at: 1108 2497 1108 Size: 1389 6835 PmeI GTTT'AAAC Cuts at: 5528 5528 Size: 8224 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3455 3487 6293 6325 7806 7838 3455 Size: 32 2806 32 1481 32 3841 Fragments arranged by size: 3841 2806 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8224 PsiI TTA'TAA Cuts at: 3015 5794 3015 Size: 2779 5445 PstI C_TGCA'G Cuts at: 830 5576 830 Size: 4746 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8224 PvuII CAG'CTG Cuts at: 3116 3116 Size: 8224 SacI G_AGCT'C Cuts at: 3236 3236 Size: 8224 SalI G'TCGA_C Cuts at: 1936 5566 1936 Size: 3630 4594 SapI GCTCTTCn'nnn_ Cuts at: 3546 3546 Size: 8224 SbfI CC_TGCA'GG Cuts at: 5576 5576 Size: 8224 ScaI AGT'ACT Cuts at: 2851 6472 8184 2851 Size: 3621 1712 2891 Fragments arranged by size: 3621 2891 1712 SfcI C'TryA_G Cuts at: 826 1045 2193 2373 3475 4230 5354 5572 6707 Size: 219 1148 180 1102 755 1124 218 1135 Cuts at: 6707 7388 7579 826 Size: 681 191 1471 Fragments arranged by size: 1471 1148 1135 1124 1102 755 681 219 218 191 180 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8224 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8224 SmaI CCC'GGG Cuts at: 1872 5551 1872 Size: 3679 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2087 2108 3452 6328 7229 7491 Size: 197 926 144 21 1344 2876 901 262 Cuts at: 7491 820 Size: 1553 Fragments arranged by size: 2876 1553 1344 926 901 262 197 144 21 SnaBI TAC'GTA Cuts at: 493 4189 6015 493 Size: 3696 1826 2702 Fragments arranged by size: 3696 2702 1826 SpeI A'CTAG_T Cuts at: 152 4870 152 Size: 4718 3506 SphI G_CATG'C Cuts at: 1132 3944 5582 1132 Size: 2812 1638 3774 Fragments arranged by size: 3774 2812 1638 SspI AAT'ATT Cuts at: 5 52 3070 4462 4973 6148 5 Size: 47 3018 1392 511 1175 2081 Fragments arranged by size: 3018 2081 1392 1175 511 47 StuI AGG'CCT Cuts at: 1517 2792 1517 Size: 1275 6949 StyI C'CwwG_G Cuts at: 513 1065 2004 5157 5663 513 Size: 552 939 3153 506 3074 Fragments arranged by size: 3153 3074 939 552 506 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2545 2871 4107 5150 6251 6268 6421 Size: 1255 965 326 1236 1043 1101 17 153 Cuts at: 6421 6606 7051 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1236 1101 1043 965 445 326 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2661 2849 3208 Size: 274 80 33 51 1443 684 188 359 Cuts at: 3208 5281 6470 8182 96 Size: 2073 1189 1712 138 Fragments arranged by size: 2073 1712 1443 1189 684 359 274 188 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2820 2852 4577 4609 8184 8216 2820 Size: 32 1725 32 3575 32 2828 Fragments arranged by size: 3575 2828 1725 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2020 1316 Size: 704 7520 XbaI T'CTAG_A Cuts at: 5560 5560 Size: 8224 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4327 4327 Size: 8224 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8224 XmaI C'CCGG_G Cuts at: 1870 5549 1870 Size: 3679 4545 XmnI GAAnn'nnTTC Cuts at: 3735 6353 3735 Size: 2618 5606 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6032 276 Size: 53 83 186 936 4498 2468 Fragments arranged by size: 4498 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI