Restriction map for
pFN21AA1033
(Linear) (Six-base) MAPSORT of: KIAA1033.seq Check: 8929 from: 1 to: 3519
KIAA1033.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 112 2984 3519
Size: 112 2872 535
Fragments arranged by size:
2872 535 112
Acc65I G'GTAC_C
Cuts at: 0 1038 3519
Size: 1038 2481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1310 3262 3384 3443 3519
Size: 766 544 1952 122 59 76
Fragments arranged by size:
1952 766 544 122 76 59
AflII C'TTAA_G
Cuts at: 0 82 3519
Size: 82 3437
AflIII A'CryG_T
Cuts at: 0 1382 1895 3519
Size: 1382 513 1624
Fragments arranged by size:
1624 1382 513
AhdI GACnn_n'nnGTC
Cuts at: 0 3340 3519
Size: 3340 179
AlwNI CAG_nnn'CTG
Cuts at: 0 438 1111 3519
Size: 438 673 2408
Fragments arranged by size:
2408 673 438
ApoI r'AATT_y
Cuts at: 0 62 97 331 401 697 704 859 950
Size: 62 35 234 70 296 7 155 91
Cuts at: 950 1282 1997 2671 2853 2946 3001 3033 3355
Size: 332 715 674 182 93 55 32 322
Cuts at: 3355 3392 3519
Size: 37 127
Fragments arranged by size:
715 674 332 322 296 234 182 155
127 93 91 70 62 55 37 35
32 7
AseI AT'TA_AT
Cuts at: 0 2453 3519
Size: 2453 1066
BanI G'GyrC_C
Cuts at: 0 1038 3519
Size: 1038 2481
BanII G_rGCy'C
Cuts at: 0 1231 3519
Size: 1231 2288
BbsI GAAGACnn'nnnn_
Cuts at: 0 1129 1827 2791 3519
Size: 1129 698 964 728
Fragments arranged by size:
1129 964 728 698
BbvCI CC'TCA_GC
Cuts at: 0 854 3519
Size: 854 2665
BciVI GTATCCnnnnn_n'
Cuts at: 0 1155 2660 3519
Size: 1155 1505 859
Fragments arranged by size:
1505 1155 859
BclI T'GATC_A
Cuts at: 0 2640 2727 2934 3519
Size: 2640 87 207 585
Fragments arranged by size:
2640 585 207 87
BfrBI ATG'CAT
Cuts at: 0 1339 1935 3519
Size: 1339 596 1584
Fragments arranged by size:
1584 1339 596
BglII A'GATC_T
Cuts at: 0 1293 2046 2788 3519
Size: 1293 753 742 731
Fragments arranged by size:
1293 753 742 731
BlpI GC'TnA_GC
Cuts at: 0 3263 3519
Size: 3263 256
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 2250 2309 3519
Size: 36 2214 59 1210
Fragments arranged by size:
2214 1210 59 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 132 3519
Size: 132 3387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 2981 3519
Size: 566 2415 538
Fragments arranged by size:
2415 566 538
Bpu10I CC'TnA_GC
Cuts at: 0 854 3519
Size: 854 2665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 1432 3519
Size: 124 1308 2087
Fragments arranged by size:
2087 1308 124
BsaI GGTCTCn'nnnn_
Cuts at: 0 349 3470 3519
Size: 349 3121 49
Fragments arranged by size:
3121 349 49
BsaAI yAC'GTr
Cuts at: 0 2184 3519
Size: 2184 1335
BsaBI GATnn'nnATC
Cuts at: 0 176 3188 3344 3519
Size: 176 3012 156 175
Fragments arranged by size:
3012 176 175 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 815 845 3519
Size: 1 30 784 30 2674
Fragments arranged by size:
2674 784 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 836 3519
Size: 121 715 2683
Fragments arranged by size:
2683 715 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1449 3519
Size: 269 1180 2070
Fragments arranged by size:
2070 1180 269
BsiHKAI G_wGCw'C
Cuts at: 0 575 1231 1610 3519
Size: 575 656 379 1909
Fragments arranged by size:
1909 656 575 379
BsmI GAATG_Cn'
Cuts at: 0 3078 3519
Size: 3078 441
Bsp1286I G_dGCh'C
Cuts at: 0 575 1231 1610 3519
Size: 575 656 379 1909
Fragments arranged by size:
1909 656 575 379
BspHI T'CATG_A
Cuts at: 0 1748 2235 3519
Size: 1748 487 1284
Fragments arranged by size:
1748 1284 487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1932 3519
Size: 1932 1587
BsrDI GCAATG_nn'
Cuts at: 0 2820 3477 3519
Size: 2820 657 42
Fragments arranged by size:
2820 657 42
BsrGI T'GTAC_A
Cuts at: 0 656 1203 3276 3519
Size: 656 547 2073 243
Fragments arranged by size:
2073 656 547 243
BstBI TT'CG_AA
Cuts at: 0 987 2481 3006 3519
Size: 987 1494 525 513
Fragments arranged by size:
1494 987 525 513
BstXI CCAn_nnnn'nTGG
Cuts at: 0 384 3159 3519
Size: 384 2775 360
Fragments arranged by size:
2775 384 360
BstYI r'GATC_y
Cuts at: 0 1293 1761 2046 2788 3519
Size: 1293 468 285 742 731
Fragments arranged by size:
1293 742 731 468 285
BstZ17I GTA'TAC
Cuts at: 0 113 3519
Size: 113 3406
Bsu36I CC'TnA_GG
Cuts at: 0 2708 3519
Size: 2708 811
BtgI C'CryG_G
Cuts at: 0 3152 3519
Size: 3152 367
BtsI GCAGTG_nn'
Cuts at: 0 3390 3519
Size: 3390 129
DraI TTT'AAA
Cuts at: 0 1716 2003 2075 3519
Size: 1716 287 72 1444
Fragments arranged by size:
1716 1444 287 72
DraIII CAC_nnn'GTG
Cuts at: 0 1690 1801 3519
Size: 1690 111 1718
Fragments arranged by size:
1718 1690 111
EaeI y'GGCC_r
Cuts at: 0 1553 3519
Size: 1553 1966
EarI CTCTTCn'nnn_
Cuts at: 0 1541 3519
Size: 1541 1978
EcoICRI GAG'CTC
Cuts at: 0 1229 3519
Size: 1229 2290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 766 1310 2981 3262 3384 3443 3519
Size: 566 200 544 1671 281 122 59 76
Fragments arranged by size:
1671 566 544 281 200 122 76 59
EcoO109I rG'GnC_Cy
Cuts at: 0 2334 3519
Size: 2334 1185
EcoRI G'AATT_C
Cuts at: 0 704 3519
Size: 704 2815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 97 922 2899 3519
Size: 97 825 1977 620
Fragments arranged by size:
1977 825 620 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 37 69 1192 1224 1594 1626
Size: 1 32 4 32 1123 32 370 32
Cuts at: 1626 1772 1804 3326 3358 3519
Size: 146 32 1522 32 161
Fragments arranged by size:
1522 1123 370 161 146 32 32 32
32 32 32 4 1
HincII GTy'rAC
Cuts at: 0 45 3519
Size: 45 3474
HindIII A'AGCT_T
Cuts at: 0 199 3175 3519
Size: 199 2976 344
Fragments arranged by size:
2976 344 199
KpnI G_GTAC'C
Cuts at: 0 1042 3519
Size: 1042 2477
MfeI C'AATT_G
Cuts at: 0 155 3519
Size: 155 3364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 927 3519
Size: 102 825 2592
Fragments arranged by size:
2592 825 102
MscI TGG'CCA
Cuts at: 0 1555 3519
Size: 1555 1964
MslI CAynn'nnrTG
Cuts at: 0 1387 1486 1702 3157 3519
Size: 1387 99 216 1455 362
Fragments arranged by size:
1455 1387 362 216 99
MspA1I CmG'CkG
Cuts at: 0 1111 3519
Size: 1111 2408
NcoI C'CATG_G
Cuts at: 0 3152 3519
Size: 3152 367
NdeI CA'TA_TG
Cuts at: 0 1463 3519
Size: 1463 2056
NsiI A_TGCA'T
Cuts at: 0 1341 1937 3519
Size: 1341 596 1582
Fragments arranged by size:
1582 1341 596
NspI r_CATG'y
Cuts at: 0 1386 1899 1939 3519
Size: 1386 513 40 1580
Fragments arranged by size:
1580 1386 513 40
PciI A'CATG_T
Cuts at: 0 1382 1895 3519
Size: 1382 513 1624
Fragments arranged by size:
1624 1382 513
PflMI CCAn_nnn'nTGG
Cuts at: 0 492 3519
Size: 492 3027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1450 1482 3519
Size: 1450 32 2037
Fragments arranged by size:
2037 1450 32
PsiI TTA'TAA
Cuts at: 0 1010 3519
Size: 1010 2509
PvuII CAG'CTG
Cuts at: 0 1111 3519
Size: 1111 2408
SacI G_AGCT'C
Cuts at: 0 1231 3519
Size: 1231 2288
SapI GCTCTTCn'nnn_
Cuts at: 0 1541 3519
Size: 1541 1978
ScaI AGT'ACT
Cuts at: 0 846 3519
Size: 846 2673
SfcI C'TryA_G
Cuts at: 0 188 368 1470 2225 3349 3519
Size: 188 180 1102 755 1124 170
Fragments arranged by size:
1124 1102 755 188 180 170
SmlI C'TyrA_G
Cuts at: 0 82 103 1447 3519
Size: 82 21 1344 2072
Fragments arranged by size:
2072 1344 82 21
SnaBI TAC'GTA
Cuts at: 0 2184 3519
Size: 2184 1335
SpeI A'CTAG_T
Cuts at: 0 2865 3519
Size: 2865 654
SphI G_CATG'C
Cuts at: 0 1939 3519
Size: 1939 1580
SspI AAT'ATT
Cuts at: 0 1065 2457 2968 3519
Size: 1065 1392 511 551
Fragments arranged by size:
1392 1065 551 511
StuI AGG'CCT
Cuts at: 0 787 3519
Size: 787 2732
StyI C'CwwG_G
Cuts at: 0 3152 3519
Size: 3152 367
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 540 866 2102 3145 3519
Size: 540 326 1236 1043 374
Fragments arranged by size:
1236 1043 540 374 326
TatI w'GTAC_w
Cuts at: 0 656 844 1203 3276 3519
Size: 656 188 359 2073 243
Fragments arranged by size:
2073 656 359 243 188
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 815 847 2572 2604 3519
Size: 815 32 1725 32 915
Fragments arranged by size:
1725 915 815 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 15 3519
Size: 15 3504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2322 3519
Size: 2322 1197
XmnI GAAnn'nnTTC
Cuts at: 0 1730 3519
Size: 1730 1789
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AhdI AlwNI ApoI
AseI BanI BanII BbsI BbvCI BciVI BclI BfrBI
BglII BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtsI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII KpnI
MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PciI PflMI PpiI PsiI PvuII SacI SapI
ScaI SfcI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AleI AlfI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BglI Bme1580I BmgBI BmtI BsaHI BsaWI
BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BssSI BstAPI BstEII BtgZI ClaI CspCI DrdI EagI
EciI EcoNI EcoRV FseI FspI FspAI HaeII HpaI
KasI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PfoI PmeI PmlI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SrfI
SwaI XbaI XhoI XmaI ZraI