(Circular) (Six-base) MAPSORT of: pFN21AA0987.seq Check: 1413 from: 1 to: 7966 pFN21AA0987 7966 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3660 4542 3660 Size: 882 7084 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5776 278 Size: 53 83 186 936 4240 2468 Fragments arranged by size: 4240 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5309 1937 Size: 3372 4594 Acc65I G'GTAC_C Cuts at: 5287 5287 Size: 7966 AclI AA'CG_TT Cuts at: 1563 3313 6093 6466 1563 Size: 1750 2780 373 3063 Fragments arranged by size: 3063 2780 1750 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2616 4368 4384 6029 7413 7681 1632 Size: 984 1752 16 1645 1384 268 1917 Fragments arranged by size: 1917 1752 1645 1384 984 268 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7769 AflIII A'CryG_T Cuts at: 1203 5202 6865 7842 1203 Size: 3999 1663 977 1327 Fragments arranged by size: 3999 1663 1327 977 AgeI A'CCGG_T Cuts at: 6797 6797 Size: 7966 AhdI GACnn_n'nnGTC Cuts at: 2644 6695 2644 Size: 4051 3915 AleI CACnn'nnGTG Cuts at: 1166 2174 3404 4602 1166 Size: 1008 1230 1198 4530 Fragments arranged by size: 4530 1230 1198 1008 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2192 4335 1184 Size: 1008 2143 4815 Fragments arranged by size: 4815 2143 1008 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4326 4358 4326 Size: 32 7934 AlwNI CAG_nnn'CTG Cuts at: 2137 2582 4250 4766 5161 5709 7281 2137 Size: 445 1668 516 395 548 1572 2822 Fragments arranged by size: 2822 1668 1572 548 516 445 395 ApaLI G'TGCA_C Cuts at: 4526 4595 5019 6023 7179 4526 Size: 69 424 1004 1156 5313 Fragments arranged by size: 5313 1156 1004 424 69 ApoI r'AATT_y Cuts at: 1493 2564 3197 4433 4911 5275 5501 5653 7762 Size: 1071 633 1236 478 364 226 152 2109 Cuts at: 7762 1493 Size: 1697 Fragments arranged by size: 2109 1697 1236 1071 633 478 364 226 152 AseI AT'TA_AT Cuts at: 160 1032 6520 160 Size: 872 5488 1606 Fragments arranged by size: 5488 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7966 AvaI C'yCGr_G Cuts at: 1870 1943 4048 5291 1870 Size: 73 2105 1243 4545 Fragments arranged by size: 4545 2105 1243 73 AvrII C'CTAG_G Cuts at: 3700 3700 Size: 7966 BamHI G'GATC_C Cuts at: 5296 5663 5296 Size: 367 7599 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2095 5287 6742 618 Size: 325 217 627 308 3192 1455 1842 Fragments arranged by size: 3192 1842 1455 627 325 308 217 BanII G_rGCy'C Cuts at: 1391 2061 2693 2715 3588 3666 4011 4152 4688 Size: 670 632 22 873 78 345 141 536 Cuts at: 4688 5285 1391 Size: 597 4072 Fragments arranged by size: 4072 873 670 632 597 536 345 141 78 22 BbeI G_GCGC'C Cuts at: 2099 2099 Size: 7966 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 2802 4356 4865 928 Size: 971 360 543 1554 509 4029 Fragments arranged by size: 4029 1554 971 543 509 360 BbvCI CC'TCA_GC Cuts at: 3426 3426 Size: 7966 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4909 4943 6157 6191 1903 Size: 34 2972 34 1214 34 3678 Fragments arranged by size: 3678 2972 1214 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5858 7068 3809 Size: 2049 1210 4707 Fragments arranged by size: 4707 2049 1210 BclI T'GATC_A Cuts at: 1548 3396 1548 Size: 1848 6118 BfrBI ATG'CAT Cuts at: 2857 2857 Size: 7966 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6577 136 Size: 107 122 71 631 605 4905 1525 Fragments arranged by size: 4905 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4993 7961 4993 Size: 2968 4998 BlpI GC'TnA_GC Cuts at: 3645 4541 5383 3645 Size: 896 842 6228 Fragments arranged by size: 6228 896 842 Bme1580I G_kGCm'C Cuts at: 1790 3568 3906 4530 4599 5023 6027 7183 1790 Size: 1778 338 624 69 424 1004 1156 2573 Fragments arranged by size: 2573 1778 1156 1004 624 424 338 69 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7966 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5697 6655 442 Size: 416 53 83 399 24 4280 958 1753 Fragments arranged by size: 4280 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7966 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2407 2407 Size: 7966 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2377 3678 4025 5018 6626 1239 Size: 920 218 1301 347 993 1608 2579 Fragments arranged by size: 2579 1608 1301 993 920 347 218 Bpu10I CC'TnA_GC Cuts at: 2508 2913 3426 5166 2508 Size: 405 513 1740 5308 Fragments arranged by size: 5308 1740 513 405 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4045 4136 4161 6091 6956 7254 4045 Size: 91 25 1930 865 298 4757 Fragments arranged by size: 4757 1930 865 298 91 25 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2304 4103 4559 6629 882 Size: 617 805 1799 456 2070 2219 Fragments arranged by size: 2219 2070 1799 805 617 456 BsaAI yAC'GTr Cuts at: 493 1204 2466 5757 493 Size: 711 1262 3291 2702 Fragments arranged by size: 3291 2702 1262 711 BsaBI GATnn'nnATC Cuts at: 2904 7960 2904 Size: 5056 2910 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2096 2837 5773 6155 Size: 53 83 186 936 563 741 2936 382 Cuts at: 6155 6790 275 Size: 635 1451 Fragments arranged by size: 2936 1451 936 741 635 563 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2648 3412 6399 6797 7071 7218 7629 2648 Size: 764 2987 398 274 147 411 2985 Fragments arranged by size: 2987 2985 764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3394 3424 4556 4586 5250 Size: 30 165 30 1991 30 1132 30 664 Cuts at: 5250 5280 1178 Size: 30 3864 Fragments arranged by size: 3864 1991 1132 664 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 3443 3446 4595 4739 4802 4911 1186 Size: 949 1308 3 1149 144 63 109 4241 Fragments arranged by size: 4241 1308 1149 949 144 109 63 3 BseYI C'CCAG_C Cuts at: 2686 3280 4467 7169 7665 2686 Size: 594 1187 2702 496 2987 Fragments arranged by size: 2987 2702 1187 594 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2798 4580 4818 1163 Size: 573 1062 1782 238 4311 Fragments arranged by size: 4311 1782 1062 573 238 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2980 3062 5416 6177 7205 Size: 275 186 12 967 82 2354 761 1028 Cuts at: 7205 1540 Size: 2301 Fragments arranged by size: 2354 2301 1028 967 761 275 186 82 12 BsiHKAI G_wGCw'C Cuts at: 2715 3588 3651 4152 4530 4599 5023 6027 6112 Size: 873 63 501 378 69 424 1004 85 Cuts at: 6112 7183 2715 Size: 1071 3498 Fragments arranged by size: 3498 1071 1004 873 501 424 378 85 69 63 BsmI GAATG_Cn' Cuts at: 4658 5477 5570 4658 Size: 819 93 7054 Fragments arranged by size: 7054 819 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4505 4505 Size: 7966 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2693 2715 3568 3588 3651 3666 Size: 399 271 632 22 853 20 63 15 Cuts at: 3666 3906 4011 4152 4530 4599 4688 5023 5285 Size: 240 105 141 378 69 89 335 262 Cuts at: 5285 6027 6112 7183 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 853 742 632 399 378 335 271 262 240 141 105 89 85 69 63 22 20 15 BspEI T'CCGG_A Cuts at: 2648 3412 7629 2648 Size: 764 4217 2985 Fragments arranged by size: 4217 2985 764 BspHI T'CATG_A Cuts at: 5855 7585 5855 Size: 1730 6236 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3660 4542 5321 844 Size: 2816 882 779 3489 Fragments arranged by size: 3489 2816 882 779 BsrBI CCG'CTC Cuts at: 1394 1823 3436 3888 4585 5853 1394 Size: 429 1613 452 697 1268 3507 Fragments arranged by size: 3507 1613 1268 697 452 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6461 6635 65 Size: 1183 5213 174 1396 Fragments arranged by size: 5213 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2979 6610 6797 1700 Size: 110 1169 3631 187 2869 Fragments arranged by size: 3631 2869 1169 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7966 BssHII G'CGCG_C Cuts at: 1925 2086 1925 Size: 161 7805 BssSI C'ACGA_G Cuts at: 3500 6026 7038 3500 Size: 2526 1012 4428 Fragments arranged by size: 4428 2526 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2337 4534 794 Size: 1543 2197 4226 Fragments arranged by size: 4226 2197 1543 BstBI TT'CG_AA Cuts at: 2530 6770 2530 Size: 4240 3726 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7966 BstXI CCAn_nnnn'nTGG Cuts at: 5221 5221 Size: 7966 BstYI r'GATC_y Cuts at: 1972 3054 4993 5296 5663 6048 6065 7517 7603 Size: 1082 1939 303 367 385 17 1452 86 Cuts at: 7603 7615 7961 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1939 1452 1082 385 367 346 303 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7414 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7227 Fragments arranged by size: 7227 683 56 BtsI GCAGTG_nn' Cuts at: 2342 4248 4888 5482 6276 6296 2342 Size: 1906 640 594 794 20 4012 Fragments arranged by size: 4012 1906 794 640 594 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7966 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7931 DraI TTT'AAA Cuts at: 5270 5270 Size: 7966 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4360 6973 809 Size: 507 3044 2613 1802 Fragments arranged by size: 3044 2613 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3059 3355 3489 Size: 54 1425 50 275 18 1229 296 134 Cuts at: 3489 4302 5413 6302 7931 8 Size: 813 1111 889 1629 43 Fragments arranged by size: 1629 1425 1229 1111 889 813 296 275 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3059 5413 1537 Size: 275 1247 2354 4090 Fragments arranged by size: 4090 2354 1247 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3250 3441 3920 4062 4268 4344 4749 5896 Size: 1897 191 479 142 206 76 405 1147 Cuts at: 5896 1353 Size: 3423 Fragments arranged by size: 3423 1897 1147 479 405 206 191 142 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6553 6927 7073 387 Size: 1064 138 4964 374 146 1280 Fragments arranged by size: 4964 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2713 3586 4150 2713 Size: 873 564 6529 Fragments arranged by size: 6529 873 564 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2377 2616 3678 4025 4368 4384 Size: 393 527 218 239 1062 347 343 16 Cuts at: 4384 5018 6029 6626 7413 7681 1239 Size: 634 1011 597 787 268 1524 Fragments arranged by size: 1524 1062 1011 787 634 597 527 393 347 343 268 239 218 16 EcoNI CCTnn'n_nnAGG Cuts at: 3699 4748 3699 Size: 1049 6917 EcoO109I rG'GnC_Cy Cuts at: 2134 2134 Size: 7966 EcoRI G'AATT_C Cuts at: 4433 5275 5653 4433 Size: 842 378 6746 Fragments arranged by size: 6746 842 378 EcoRV GAT'ATC Cuts at: 2602 2602 Size: 7966 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2366 2556 2366 Size: 190 7776 FspI TGC'GCA Cuts at: 6472 6472 Size: 7966 HaeII r_GCGC'y Cuts at: 1678 2099 4032 5162 7113 1678 Size: 421 1933 1130 1951 2531 Fragments arranged by size: 2531 1951 1933 1130 421 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2625 2630 2657 Size: 32 135 32 446 32 575 5 27 Cuts at: 2657 2662 3494 3526 4180 4212 6607 6639 6681 Size: 5 832 32 654 32 2395 32 42 Cuts at: 6681 6713 1373 Size: 32 2626 Fragments arranged by size: 2626 2395 832 654 575 446 135 42 32 32 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 5310 5556 669 Size: 988 281 3372 246 3079 Fragments arranged by size: 3372 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7966 HpaI GTT'AAC Cuts at: 5556 5556 Size: 7966 KasI G'GCGC_C Cuts at: 2095 2095 Size: 7966 KpnI G_GTAC'C Cuts at: 5291 5291 Size: 7966 MfeI C'AATT_G Cuts at: 5565 5565 Size: 7966 MluI A'CGCG_T Cuts at: 7842 7842 Size: 7966 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2478 4107 7080 7264 1305 Size: 1173 1629 2973 184 2007 Fragments arranged by size: 2973 2007 1629 1173 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3357 10 Size: 54 1425 343 1525 4619 Fragments arranged by size: 4619 1525 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 3404 4602 5207 5924 6283 6442 Size: 648 1008 1230 1198 605 717 359 159 Cuts at: 6442 518 Size: 2042 Fragments arranged by size: 2042 1230 1198 1008 717 648 605 359 159 MspA1I CmG'CkG Cuts at: 1606 2156 4635 4766 5382 6059 7207 7452 1606 Size: 550 2479 131 616 677 1148 245 2120 Fragments arranged by size: 2479 2120 1148 677 616 550 245 131 NarI GG'CG_CC Cuts at: 2096 2096 Size: 7966 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7414 NdeI CA'TA_TG Cuts at: 387 4963 387 Size: 4576 3390 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7966 NotI GC'GGCC_GC Cuts at: 5413 5413 Size: 7966 NruI TCG'CGA Cuts at: 2976 2976 Size: 7966 NsiI A_TGCA'T Cuts at: 2859 2859 Size: 7966 NspI r_CATG'y Cuts at: 1132 1229 2335 5206 5324 6869 1132 Size: 97 1106 2871 118 1545 2229 Fragments arranged by size: 2871 2229 1545 1106 118 97 PasI CC'CwG_GG Cuts at: 1348 3686 1348 Size: 2338 5628 PciI A'CATG_T Cuts at: 5202 6865 5202 Size: 1663 6303 PflMI CCAn_nnn'nTGG Cuts at: 1108 5161 5255 1108 Size: 4053 94 3819 Fragments arranged by size: 4053 3819 94 PfoI T'CCnGG_A Cuts at: 4743 4743 Size: 7966 PmeI GTTT'AAAC Cuts at: 5270 5270 Size: 7966 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4042 4074 6035 6067 7548 7580 4042 Size: 32 1961 32 1481 32 4428 Fragments arranged by size: 4428 1961 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3534 1613 Size: 1921 6045 PsiI TTA'TAA Cuts at: 5536 5536 Size: 7966 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3029 3061 3029 Size: 32 7934 PstI C_TGCA'G Cuts at: 830 2161 4648 5318 830 Size: 1331 2487 670 3478 Fragments arranged by size: 3478 2487 1331 670 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7966 SacI G_AGCT'C Cuts at: 2715 3588 4152 2715 Size: 873 564 6529 Fragments arranged by size: 6529 873 564 SalI G'TCGA_C Cuts at: 1936 5308 1936 Size: 3372 4594 SapI GCTCTTCn'nnn_ Cuts at: 3250 3250 Size: 7966 SbfI CC_TGCA'GG Cuts at: 5318 5318 Size: 7966 ScaI AGT'ACT Cuts at: 6214 7926 6214 Size: 1712 6254 SexAI A'CCwGG_T Cuts at: 2606 2606 Size: 7966 SfcI C'TryA_G Cuts at: 826 1045 2157 3727 4644 5314 6449 7130 7321 Size: 219 1112 1570 917 670 1135 681 191 Cuts at: 7321 826 Size: 1471 Fragments arranged by size: 1570 1471 1135 1112 917 681 670 219 191 SfoI GGC'GCC Cuts at: 2097 2097 Size: 7966 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7966 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7966 SmaI CCC'GGG Cuts at: 1872 5293 1872 Size: 3421 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4024 4151 4176 6070 6971 7233 Size: 197 926 2081 127 25 1894 901 262 Cuts at: 7233 820 Size: 1553 Fragments arranged by size: 2081 1894 1553 926 901 262 197 127 25 SnaBI TAC'GTA Cuts at: 493 2466 5757 493 Size: 1973 3291 2702 Fragments arranged by size: 3291 2702 1973 SpeI A'CTAG_T Cuts at: 152 152 Size: 7966 SphI G_CATG'C Cuts at: 1132 2335 5324 1132 Size: 1203 2989 3774 Fragments arranged by size: 3774 2989 1203 SspI AAT'ATT Cuts at: 5 52 5890 5 Size: 47 5838 2081 Fragments arranged by size: 5838 2081 47 StuI AGG'CCT Cuts at: 1517 3694 1517 Size: 2177 5789 StyI C'CwwG_G Cuts at: 513 1065 2115 3009 3207 3669 3700 4011 5405 Size: 552 1050 894 198 462 31 311 1394 Cuts at: 5405 513 Size: 3074 Fragments arranged by size: 3074 1394 1050 894 552 462 311 198 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2027 2286 3572 5993 6010 6163 6348 Size: 1255 447 259 1286 2421 17 153 185 Cuts at: 6348 6793 325 Size: 445 1498 Fragments arranged by size: 2421 1498 1286 1255 447 445 259 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6212 7924 96 Size: 274 80 33 51 1443 4235 1712 138 Fragments arranged by size: 4235 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3127 3159 3394 3426 7926 7958 3127 Size: 32 235 32 4500 32 3135 Fragments arranged by size: 4500 3135 235 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4495 1316 Size: 3179 4787 XbaI T'CTAG_A Cuts at: 5302 5302 Size: 7966 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7966 XmaI C'CCGG_G Cuts at: 1870 5291 1870 Size: 3421 4545 XmnI GAAnn'nnTTC Cuts at: 6095 6095 Size: 7966 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5774 276 Size: 53 83 186 936 4240 2468 Fragments arranged by size: 4240 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BsiWI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SfiI SrfI SwaI XcmI