Restriction map for
pFN21AA0901
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AviII BbeI BciVI BfrI
BglII BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoT22I FseI FspI HindIII
HpaI KasI KspI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1506 GGTACC G^GTACC
AccI 3451 GTCTAC GT^[AC][GT]AC
AcyI 1180 GGCGTC G[GA]^CG[CT]C
AflIII 1973 ACATGT A^C[GA][CT]GT
Asp718I 1506 GGTACC G^GTACC
AspI 3018 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2581 CCTAGG C^CTAGG
BbrPI 688 CACGTG CAC^GTG
BlnI 2581 CCTAGG C^CTAGG
BsaHI 1180 GGCGTC G[GA]^CG[CT]C
BsiEI 2073 CGGCCG CG[GA][CT]^CG
BspHI 2507 TCATGA T^CATGA
BspLU11I 1973 ACATGT A^CATGT
BsrFI 10 ACCGGC [GA]^CCGG[CT]
BssSI 2606 CACGAG CACGAG(-5/-1)
BstBI 90 TTCGAA TT^CGAA
BstEII 2550 GGTCACC G^GT[ACGT]ACC
BstPI 2550 GGTCACC G^GT[ACGT]ACC
Cfr10I 10 ACCGGC [GA]^CCGG[CT]
DraIII 1235 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2073 CGGCCG C^GGCCG
EclXI 2073 CGGCCG C^GGCCG
Eco52I 2073 CGGCCG C^GGCCG
EcoO65I 2550 GGTCACC G^GT[ACGT]ACC
EcoRI 286 GAATTC G^AATTC
EcoRV 3129 GATATC GAT^ATC
Hin1I 1180 GGCGTC G[GA]^CG[CT]C
HincII 2101 GTCAAC GT[CT]^[GA]AC
HindII 2101 GTCAAC GT[CT]^[GA]AC
KpnI 1506 GGTACC GGTAC^C
NspV 90 TTCGAA TT^CGAA
PflFI 3018 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 688 CACGTG CAC^GTG
PmlI 688 CACGTG CAC^GTG
PstI 3368 CTGCAG CTGCA^G
RcaI 2507 TCATGA T^CATGA
SapI 415 GAAGAGC GCTCTTC(1/4)
SfuI 90 TTCGAA TT^CGAA
SmaI 1667 CCCGGG CCC^GGG
SphI 1914 GCATGC GCATG^C
SspI 100 AATATT AAT^ATT
Tth111I 3018 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1667 CCCGGG C^CCGGG
XmaI 1667 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccII 1165 CGCG CG^CG
1583 CGCG
AcsI 286 GAATTC [GA]^AATT[CT]
3498 AAATTC
Alw44I 760 GTGCAC G^TGCAC
1733 GTGCAC
ApaI 2119 GGGCCC GGGCC^C
2444 GGGCCC
ApaLI 760 GTGCAC G^TGCAC
1733 GTGCAC
ApoI 286 GAATTC [GA]^AATT[CT]
3498 AAATTC
BamHI 1925 GGATCC G^GATCC
2238 GGATCC
BbsI 193 GAAGAC GAAGAC(2/6)
2518 GAAGAC
BbvCI 1006 CCTCAGC CCTCAGC(-5/-2)
3519 CCTCAGC
BcgI 3266 CGATGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2063 GCAGCCAGATCG
BclI 2022 TGATCA T^GATCA
3297 TGATCA
BlpI 1600 GCTGAGC GC^T[ACGT]AGC
2590 GCTGAGC
Bpu1102I 1600 GCTGAGC GC^T[ACGT]AGC
2590 GCTGAGC
BpuAI 193 GAAGAC GAAGAC(2/6)
2518 GAAGAC
BsaAI 688 CACGTG [CT]AC^GT[GA]
1630 TACGTG
BstAPI 972 GCACGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1735 GCACAGCTTGC
BstUI 1165 CGCG CG^CG
1583 CGCG
CelII 1600 GCTGAGC GC^T[ACGT]AGC
2590 GCTGAGC
DrdI 76 GACTCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
799 GACCCCAGTGTC
EarI 415 GAAGAG CTCTTC(1/4)
2665 GAAGAG
FbaI 2022 TGATCA T^GATCA
3297 TGATCA
HaeII 1562 GGCGCT [GA]GCGC^[CT]
1750 GGCGCT
HgaI 1181 GCGTC GACGC(5/10)
1815 GCGTC
Ksp632I 415 GAAGAG CTCTTC(1/4)
2665 GAAGAG
MvnI 1165 CGCG CG^CG
1583 CGCG
PaeR7I 994 CTCGAG C^TCGAG
3072 CTCGAG
PflMI 180 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3342 CCATTTGGTGG
SexAI 929 ACCAGGT A^CC[AT]GGT
1236 ACCTGGT
Van91I 180 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3342 CCATTTGGTGG
XhoI 994 CTCGAG C^TCGAG
3072 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII