Restriction map for
pFN21AA0829
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI Asp718I AspEI AspI AvrII
BanI BbeI BbrPI BbvCI BcgI
BcnI BglI BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII Cfr10I CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI FauI FseI HapII
HgaI HpaII KasI KpnI KspI
MluI MroI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SwaI Tth111I
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 407 GACGTC GACGT^C
AciI 3625 GCGG CCGC(-3/-1)
AcyI 407 GACGTC G[GA]^CG[CT]C
AocI 859 CCTAAGG CC^T[ACGT]AGG
ApaI 1400 GGGCCC GGGCC^C
AseI 3117 ATTAAT AT^TAAT
AsnI 3117 ATTAAT AT^TAAT
AvaI 2420 CTCGAG C^[CT]CG[GA]G
AviII 2327 TGCGCA TGC^GCA
BamHI 2194 GGATCC G^GATCC
BsaBI 988 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 407 GACGTC G[GA]^CG[CT]C
BsaI 1586 GAGACC GGTCTC(1/5)
BsoBI 2420 CTCGAG C^[CT]CG[GA]G
BspDI 1483 ATCGAT AT^CGAT
BstEII 1910 GGTCACC G^GT[ACGT]ACC
BstPI 1910 GGTCACC G^GT[ACGT]ACC
Bsu36I 859 CCTAAGG CC^T[ACGT]AGG
ClaI 1483 ATCGAT AT^CGAT
DsaI 2902 CCACGG C^C[GA][CT]GG
Eco81I 859 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1910 GGTCACC G^GT[ACGT]ACC
FspI 2327 TGCGCA TGC^GCA
HaeII 7 AGCGCC [GA]GCGC^[CT]
Hin1I 407 GACGTC G[GA]^CG[CT]C
HindIII 2588 AAGCTT A^AGCTT
MamI 988 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 257 CAATTG C^AATTG
MunI 257 CAATTG C^AATTG
PaeR7I 2420 CTCGAG C^TCGAG
PflMI 2277 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3280 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3117 ATTAAT AT^TAAT
SapI 1312 GCTCTTC GCTCTTC(1/4)
SexAI 1870 ACCAGGT A^CC[AT]GGT
SnaBI 1504 TACGTA TAC^GTA
SspI 895 AATATT AAT^ATT
StuI 1933 AGGCCT AGG^CCT
Van91I 2277 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2420 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflII 2550 CTTAAG C^TTAAG
2908 CTTAAG
AflIII 1367 ACATGT A^C[GA][CT]GT
3454 ACATGT
BalI 597 TGGCCA TGG^CCA
2132 TGGCCA
BclI 993 TGATCA T^GATCA
1743 TGATCA
BfrI 2550 CTTAAG C^TTAAG
2908 CTTAAG
BglII 636 AGATCT A^GATCT
1698 AGATCT
BpmI 353 CTCCAG CTGGAG(16/14)
1565 CTCCAG
BsaAI 1504 TACGTA [CT]AC^GT[GA]
3446 TACGTG
BseRI 1526 CTCCTC GAGGAG(10/8)
2172 CTCCTC
BsmFI 1779 GGGAC GGGAC(10/14)
2439 GGGAC
BspLU11I 1367 ACATGT A^CATGT
3454 ACATGT
BstXI 26 CCAATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
113 CCATCAAGTTGG
CfoI 8 GCGC GCG^C
2328 GCGC
DraI 1147 TTTAAA TTT^AAA
3211 TTTAAA
DraII 1399 GGGGCCC [GA]G^G[ACGT]CC[CT]
1400 GGGCCCT
EaeI 597 TGGCCA [CT]^GGCC[GA]
2132 TGGCCA
EcoO109I 1399 GGGGCCC [GA]G^G[ACGT]CC[CT]
1400 GGGCCCT
EcoRI 1108 GAATTC G^AATTC
3589 GAATTC
EcoRV 2922 GATATC GAT^ATC
3301 GATATC
EcoT22I 2116 ATGCAT ATGCA^T
2633 ATGCAT
FbaI 993 TGATCA T^GATCA
1743 TGATCA
HhaI 8 GCGC GCG^C
2328 GCGC
HinP1I 8 GCGC G^CGC
2328 GCGC
HpaI 1371 GTTAAC GTT^AAC
1875 GTTAAC
MluNI 597 TGGCCA TGG^CCA
2132 TGGCCA
MscI 597 TGGCCA TGG^CCA
2132 TGGCCA
NdeI 2780 CATATG CA^TATG
2936 CATATG
NsiI 2116 ATGCAT ATGCA^T
2633 ATGCAT
PleI 1287 GAGTC GAGTC(4/5)
3573 GAGTC
PstI 100 CTGCAG CTGCA^G
3415 CTGCAG
SacI 2086 GAGCTC GAGCT^C
2249 GAGCTC
ScaI 818 AGTACT AGT^ACT
3411 AGTACT
SpeI 2698 ACTAGT A^CTAGT
3685 ACTAGT
SphI 2330 GCATGC GCATG^C
2425 GCATGC
XbaI 2079 TCTAGA T^CTAGA
3276 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BciVI BfaI BmyI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI