Restriction map for
pFN21AA0829
(Linear) (Six-base) MAPSORT of: KIAA0829.seq Check: 8347 from: 1 to: 3690
KIAA0829.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AatII G_ACGT'C
Cuts at: 0 411 3690
Size: 411 3279
AccI GT'mk_AC
Cuts at: 0 537 2488 3391 3690
Size: 537 1951 903 299
Fragments arranged by size:
1951 903 537 299
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 1631 1819 3690
Size: 1267 364 188 1871
Fragments arranged by size:
1871 1267 364 188
AflII C'TTAA_G
Cuts at: 0 2550 2908 3690
Size: 2550 358 782
Fragments arranged by size:
2550 782 358
AflIII A'CryG_T
Cuts at: 0 1367 3454 3690
Size: 1367 2087 236
Fragments arranged by size:
2087 1367 236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 298 3690
Size: 298 3392
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1937 1969 3690
Size: 1937 32 1721
Fragments arranged by size:
1937 1721 32
ApaI G_GGCC'C
Cuts at: 0 1404 3690
Size: 1404 2286
ApoI r'AATT_y
Cuts at: 0 195 784 1108 1473 2968 3310 3589 3690
Size: 195 589 324 365 1495 342 279 101
Fragments arranged by size:
1495 589 365 342 324 279 195 101
AseI AT'TA_AT
Cuts at: 0 3118 3690
Size: 3118 572
AvaI C'yCGr_G
Cuts at: 0 2420 3690
Size: 2420 1270
BamHI G'GATC_C
Cuts at: 0 2194 3690
Size: 2194 1496
BanII G_rGCy'C
Cuts at: 0 1404 2090 2253 3690
Size: 1404 686 163 1437
Fragments arranged by size:
1437 1404 686 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 1351 1527 3053 3690
Size: 1351 176 1526 637
Fragments arranged by size:
1526 1351 637 176
BciVI GTATCCnnnnn_n'
Cuts at: 0 2305 3129 3652 3690
Size: 2305 824 523 38
Fragments arranged by size:
2305 824 523 38
BclI T'GATC_A
Cuts at: 0 993 1743 3690
Size: 993 750 1947
Fragments arranged by size:
1947 993 750
BfrBI ATG'CAT
Cuts at: 0 2118 2635 3690
Size: 2118 517 1055
Fragments arranged by size:
2118 1055 517
BglII A'GATC_T
Cuts at: 0 636 1698 3690
Size: 636 1062 1992
Fragments arranged by size:
1992 1062 636
Bme1580I G_kGCm'C
Cuts at: 0 1404 3690
Size: 1404 2286
BmgBI CAC'GTC
Cuts at: 0 1051 3690
Size: 1051 2639
BmrI ACTGGGnnnn_n'
Cuts at: 0 540 2011 3690
Size: 540 1471 1679
Fragments arranged by size:
1679 1471 540
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2381 3690
Size: 2381 1309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 1550 3690
Size: 338 1212 2140
Fragments arranged by size:
2140 1212 338
Bpu10I CC'TnA_GC
Cuts at: 0 3617 3690
Size: 3617 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1514 2466 3690
Size: 778 736 952 1224
Fragments arranged by size:
1224 952 778 736
BsaI GGTCTCn'nnnn_
Cuts at: 0 1580 3690
Size: 1580 2110
BsaAI yAC'GTr
Cuts at: 0 1506 3448 3690
Size: 1506 1942 242
Fragments arranged by size:
1942 1506 242
BsaBI GATnn'nnATC
Cuts at: 0 992 3690
Size: 992 2698
BsaHI Gr'CG_yC
Cuts at: 0 408 3690
Size: 408 3282
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1110 1140 1937 1967 3690
Size: 1110 30 797 30 1723
Fragments arranged by size:
1723 1110 797 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1517 2163 3690
Size: 1517 646 1527
Fragments arranged by size:
1527 1517 646
BseYI C'CCAG_C
Cuts at: 0 1906 3690
Size: 1906 1784
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1073 1115 2846 3428 3690
Size: 258 815 42 1731 582 262
Fragments arranged by size:
1731 815 582 262 258 42
BsiHKAI G_wGCw'C
Cuts at: 0 2090 2253 2362 3690
Size: 2090 163 109 1328
Fragments arranged by size:
2090 1328 163 109
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1041 1128 2509 3690
Size: 1041 87 1381 1181
Fragments arranged by size:
1381 1181 1041 87
Bsp1286I G_dGCh'C
Cuts at: 0 1404 2090 2253 2362 3690
Size: 1404 686 163 109 1328
Fragments arranged by size:
1404 1328 686 163 109
BsrDI GCAATG_nn'
Cuts at: 0 798 971 1256 2393 3690
Size: 798 173 285 1137 1297
Fragments arranged by size:
1297 1137 798 285 173
BsrGI T'GTAC_A
Cuts at: 0 1221 3266 3457 3690
Size: 1221 2045 191 233
Fragments arranged by size:
2045 1221 233 191
BstAPI GCAn_nnn'nTGC
Cuts at: 0 379 970 1098 2632 3690
Size: 379 591 128 1534 1058
Fragments arranged by size:
1534 1058 591 379 128
BstEII G'GTnAC_C
Cuts at: 0 1910 3690
Size: 1910 1780
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 120 3690
Size: 33 87 3570
Fragments arranged by size:
3570 87 33
BstYI r'GATC_y
Cuts at: 0 636 1698 2194 3126 3690
Size: 636 1062 496 932 564
Fragments arranged by size:
1062 932 636 564 496
Bsu36I CC'TnA_GG
Cuts at: 0 860 3690
Size: 860 2830
BtgI C'CryG_G
Cuts at: 0 2902 3690
Size: 2902 788
BtsI GCAGTG_nn'
Cuts at: 0 575 3690
Size: 575 3115
ClaI AT'CG_AT
Cuts at: 0 1484 3690
Size: 1484 2206
DraI TTT'AAA
Cuts at: 0 1149 3213 3690
Size: 1149 2064 477
Fragments arranged by size:
2064 1149 477
EaeI y'GGCC_r
Cuts at: 0 597 2132 3690
Size: 597 1535 1558
Fragments arranged by size:
1558 1535 597
EarI CTCTTCn'nnn_
Cuts at: 0 1154 1319 3063 3565 3690
Size: 1154 165 1744 502 125
Fragments arranged by size:
1744 1154 502 165 125
EcoICRI GAG'CTC
Cuts at: 0 2088 2251 3690
Size: 2088 163 1439
Fragments arranged by size:
2088 1439 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 1267 1550 1631 1819 3690
Size: 338 929 283 81 188 1871
Fragments arranged by size:
1871 929 338 283 188 81
EcoO109I rG'GnC_Cy
Cuts at: 0 1400 1401 3690
Size: 1400 1 2289
Fragments arranged by size:
2289 1400 1
EcoRI G'AATT_C
Cuts at: 0 1108 3589 3690
Size: 1108 2481 101
Fragments arranged by size:
2481 1108 101
EcoRV GAT'ATC
Cuts at: 0 2924 3303 3690
Size: 2924 379 387
Fragments arranged by size:
2924 387 379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 319 517 1636 2934 3690
Size: 319 198 1119 1298 756
Fragments arranged by size:
1298 1119 756 319 198
FspI TGC'GCA
Cuts at: 0 2329 3690
Size: 2329 1361
HaeII r_GCGC'y
Cuts at: 0 11 3690
Size: 11 3679
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 1110 1142 1477 1509 2186 2218
Size: 667 32 411 32 335 32 677 32
Cuts at: 2218 3287 3319 3643 3675 3690
Size: 1069 32 324 32 15
Fragments arranged by size:
1069 677 667 411 335 324 32 32
32 32 32 32 15
HincII GTy'rAC
Cuts at: 0 551 686 1373 1877 2704 3690
Size: 551 135 687 504 827 986
Fragments arranged by size:
986 827 687 551 504 135
HindIII A'AGCT_T
Cuts at: 0 2588 3690
Size: 2588 1102
HpaI GTT'AAC
Cuts at: 0 1373 1877 3690
Size: 1373 504 1813
Fragments arranged by size:
1813 1373 504
MfeI C'AATT_G
Cuts at: 0 257 3690
Size: 257 3433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 1018 1563 2543 3404 3690
Size: 101 917 545 980 861 286
Fragments arranged by size:
980 917 861 545 286 101
MscI TGG'CCA
Cuts at: 0 599 2134 3690
Size: 599 1535 1556
Fragments arranged by size:
1556 1535 599
MslI CAynn'nnrTG
Cuts at: 0 280 2650 3258 3690
Size: 280 2370 608 432
Fragments arranged by size:
2370 608 432 280
MspA1I CmG'CkG
Cuts at: 0 1057 1735 2059 2446 2623 3690
Size: 1057 678 324 387 177 1067
Fragments arranged by size:
1067 1057 678 387 324 177
NdeI CA'TA_TG
Cuts at: 0 2781 2937 3690
Size: 2781 156 753
Fragments arranged by size:
2781 753 156
NsiI A_TGCA'T
Cuts at: 0 2120 2637 3690
Size: 2120 517 1053
Fragments arranged by size:
2120 1053 517
NspI r_CATG'y
Cuts at: 0 285 1371 2122 2334 2429 3458 3690
Size: 285 1086 751 212 95 1029 232
Fragments arranged by size:
1086 1029 751 285 232 212 95
PciI A'CATG_T
Cuts at: 0 1367 3454 3690
Size: 1367 2087 236
Fragments arranged by size:
2087 1367 236
PflMI CCAn_nnn'nTGG
Cuts at: 0 2283 3690
Size: 2283 1407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1937 1969 3690
Size: 1937 32 1721
Fragments arranged by size:
1937 1721 32
PshAI GACnn'nnGTC
Cuts at: 0 3284 3690
Size: 3284 406
PsiI TTA'TAA
Cuts at: 0 892 932 3187 3690
Size: 892 40 2255 503
Fragments arranged by size:
2255 892 503 40
PspOMI G'GGCC_C
Cuts at: 0 1400 3690
Size: 1400 2290
PspXI vC'TCGA_Gb
Cuts at: 0 2420 3690
Size: 2420 1270
PstI C_TGCA'G
Cuts at: 0 104 3419 3690
Size: 104 3315 271
Fragments arranged by size:
3315 271 104
PvuII CAG'CTG
Cuts at: 0 1057 1735 2059 2446 2623 3690
Size: 1057 678 324 387 177 1067
Fragments arranged by size:
1067 1057 678 387 324 177
SacI G_AGCT'C
Cuts at: 0 2090 2253 3690
Size: 2090 163 1437
Fragments arranged by size:
2090 1437 163
SapI GCTCTTCn'nnn_
Cuts at: 0 1319 3690
Size: 1319 2371
ScaI AGT'ACT
Cuts at: 0 820 3413 3690
Size: 820 2593 277
Fragments arranged by size:
2593 820 277
SexAI A'CCwGG_T
Cuts at: 0 1870 3690
Size: 1870 1820
SfcI C'TryA_G
Cuts at: 0 100 1076 1880 2040 2447 2489 3415 3690
Size: 100 976 804 160 407 42 926 275
Fragments arranged by size:
976 926 804 407 275 160 100 42
SmlI C'TyrA_G
Cuts at: 0 757 1529 2420 2481 2550 2908 3690
Size: 757 772 891 61 69 358 782
Fragments arranged by size:
891 782 772 757 358 69 61
SnaBI TAC'GTA
Cuts at: 0 1506 3690
Size: 1506 2184
SpeI A'CTAG_T
Cuts at: 0 2698 3685 3690
Size: 2698 987 5
Fragments arranged by size:
2698 987 5
SphI G_CATG'C
Cuts at: 0 2334 2429 3690
Size: 2334 95 1261
Fragments arranged by size:
2334 1261 95
SspI AAT'ATT
Cuts at: 0 897 3690
Size: 897 2793
StuI AGG'CCT
Cuts at: 0 1935 3690
Size: 1935 1755
StyI C'CwwG_G
Cuts at: 0 461 1797 3077 3690
Size: 461 1336 1280 613
Fragments arranged by size:
1336 1280 613 461
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3443 3690
Size: 3443 247
TatI w'GTAC_w
Cuts at: 0 818 1221 1422 3266 3411 3457 3690
Size: 818 403 201 1844 145 46 233
Fragments arranged by size:
1844 818 403 233 201 145 46
XbaI T'CTAG_A
Cuts at: 0 2079 3276 3690
Size: 2079 1197 414
Fragments arranged by size:
2079 1197 414
XhoI C'TCGA_G
Cuts at: 0 2420 3690
Size: 2420 1270
XmnI GAAnn'nnTTC
Cuts at: 0 302 1100 1616 1952 3690
Size: 302 798 516 336 1738
Fragments arranged by size:
1738 798 516 336 302
ZraI GAC'GTC
Cuts at: 0 409 3690
Size: 409 3281
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AlfI AloI ApaI
ApoI AseI AvaI BamHI BanII BbsI BciVI BclI
BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BsrDI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI DraI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI
MslI MspA1I NdeI NsiI NspI PciI PflMI PpiI
PshAI PsiI PspOMI PspXI PstI PvuII SacI SapI
ScaI SexAI SfcI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI XbaI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AleI AlwNI
ApaLI AscI AsiSI AvrII BaeI BanI BbeI BbvCI
BcgI BglI BlpI BmtI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI
BstZ17I BtgZI CspCI DraIII DrdI EagI EciI EcoNI
FseI FspAI KasI KpnI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PfoI PmeI
PmlI PpuMI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SrfI SwaI
TstI Tth111I XcmI XmaI