(Circular) (Six-base) MAPSORT of: pFN21AA0751.seq Check: 6825 from: 1 to: 8269 pFN21AA0751 8269 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4741 5110 4741 Size: 369 7900 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6079 278 Size: 53 83 186 936 4543 2468 Fragments arranged by size: 4543 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4958 5612 1937 Size: 3021 654 4594 Fragments arranged by size: 4594 3021 654 Acc65I G'GTAC_C Cuts at: 5590 5590 Size: 8269 AclI AA'CG_TT Cuts at: 1563 4844 6396 6769 1563 Size: 3281 1552 373 3063 Fragments arranged by size: 3281 3063 1552 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2520 4109 6332 7716 7984 1632 Size: 888 1589 2223 1384 268 1917 Fragments arranged by size: 2223 1917 1589 1384 888 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8072 AflIII A'CryG_T Cuts at: 1203 3215 7168 8145 1203 Size: 2012 3953 977 1327 Fragments arranged by size: 3953 2012 1327 977 AgeI A'CCGG_T Cuts at: 7100 7100 Size: 8269 AhdI GACnn_n'nnGTC Cuts at: 4529 6998 4529 Size: 2469 5800 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8269 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8269 AlwNI CAG_nnn'CTG Cuts at: 5092 6012 7584 5092 Size: 920 1572 5777 Fragments arranged by size: 5777 1572 920 ApaI G_GGCC'C Cuts at: 5173 5173 Size: 8269 ApaLI G'TGCA_C Cuts at: 6326 7482 6326 Size: 1156 7113 ApoI r'AATT_y Cuts at: 1493 2440 3873 3989 4055 5578 5804 5956 8065 Size: 947 1433 116 66 1523 226 152 2109 Cuts at: 8065 1493 Size: 1697 Fragments arranged by size: 2109 1697 1523 1433 947 226 152 116 66 AseI AT'TA_AT Cuts at: 160 1032 3800 4703 6823 160 Size: 872 2768 903 2120 1606 Fragments arranged by size: 2768 2120 1606 903 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8269 AvaI C'yCGr_G Cuts at: 1870 1943 2508 2673 3351 5594 1870 Size: 73 565 165 678 2243 4545 Fragments arranged by size: 4545 2243 678 565 165 73 BamHI G'GATC_C Cuts at: 2490 5599 5966 2490 Size: 3109 367 4793 Fragments arranged by size: 4793 3109 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5590 7045 618 Size: 325 217 627 3803 1455 1842 Fragments arranged by size: 3803 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4205 5173 5588 1391 Size: 2814 968 415 4072 Fragments arranged by size: 4072 2814 968 415 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 3514 4189 928 Size: 971 393 1222 675 5008 Fragments arranged by size: 5008 1222 971 675 393 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3081 3115 6460 6494 1903 Size: 34 50 34 1060 34 3345 34 3678 Fragments arranged by size: 3678 3345 1060 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2209 6161 7371 2209 Size: 3952 1210 3107 Fragments arranged by size: 3952 3107 1210 BclI T'GATC_A Cuts at: 1548 2293 1548 Size: 745 7524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6880 136 Size: 107 122 71 631 605 5208 1525 Fragments arranged by size: 5208 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2231 2612 3826 4312 4715 8264 2231 Size: 381 1214 486 403 3549 2236 Fragments arranged by size: 3549 2236 1214 486 403 381 BlpI GC'TnA_GC Cuts at: 5070 5686 5070 Size: 616 7653 Bme1580I G_kGCm'C Cuts at: 1790 5173 6330 7486 1790 Size: 3383 1157 1156 2573 Fragments arranged by size: 3383 2573 1157 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8269 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4422 4770 6000 Size: 416 53 83 399 24 3005 348 1230 Cuts at: 6000 6958 442 Size: 958 1753 Fragments arranged by size: 3005 1753 1230 958 416 399 348 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8269 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3665 3957 4536 4636 4776 5171 6929 1239 Size: 2426 292 579 100 140 395 1758 2579 Fragments arranged by size: 2579 2426 1758 579 395 292 140 100 Bpu10I CC'TnA_GC Cuts at: 2585 2682 2585 Size: 97 8172 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2791 4652 6394 7259 7557 2791 Size: 1861 1742 865 298 3503 Fragments arranged by size: 3503 1861 1742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2896 4386 4752 6932 882 Size: 617 1397 1490 366 2180 2219 Fragments arranged by size: 2219 2180 1490 1397 617 366 BsaAI yAC'GTr Cuts at: 493 1204 3216 6060 493 Size: 711 2012 2844 2702 Fragments arranged by size: 2844 2702 2012 711 BsaBI GATnn'nnATC Cuts at: 2236 2617 3294 4606 8263 2236 Size: 381 677 1312 3657 2242 Fragments arranged by size: 3657 2242 1312 677 381 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5083 6076 6458 7093 Size: 53 83 186 936 3550 993 382 635 Cuts at: 7093 275 Size: 1451 Fragments arranged by size: 3550 1451 993 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4490 6702 7100 7374 7521 7932 4490 Size: 2212 398 274 147 411 4827 Fragments arranged by size: 4827 2212 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4611 4641 5485 5515 1178 Size: 30 165 30 3208 30 844 30 3932 Fragments arranged by size: 3932 3208 844 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2755 2990 3254 4374 4428 5493 1186 Size: 1569 235 264 1120 54 1065 3962 Fragments arranged by size: 3962 1569 1120 1065 264 235 54 BseYI C'CCAG_C Cuts at: 7472 7968 7472 Size: 496 7773 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7696 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5719 6480 7508 1540 Size: 275 186 3718 761 1028 2301 Fragments arranged by size: 3718 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2515 4883 6330 6415 7486 2515 Size: 2368 1447 85 1071 3298 Fragments arranged by size: 3298 2368 1447 1071 85 BsmI GAATG_Cn' Cuts at: 2335 4008 5780 5873 2335 Size: 1673 1772 93 4731 Fragments arranged by size: 4731 1772 1673 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2515 4205 4883 5173 5588 6330 6415 Size: 399 725 1690 678 290 415 742 85 Cuts at: 6415 7486 1391 Size: 1071 2174 Fragments arranged by size: 2174 1690 1071 742 725 678 415 399 290 85 BspEI T'CCGG_A Cuts at: 7932 7932 Size: 8269 BspHI T'CATG_A Cuts at: 4683 6158 7888 4683 Size: 1475 1730 5064 Fragments arranged by size: 5064 1730 1475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4741 5110 5624 844 Size: 3897 369 514 3489 Fragments arranged by size: 3897 3489 514 369 BsrBI CCG'CTC Cuts at: 1394 1823 4636 4650 6156 1394 Size: 429 2813 14 1506 3507 Fragments arranged by size: 3507 2813 1506 429 14 BsrDI GCAATG_nn' Cuts at: 65 1248 2018 2229 3372 4145 5112 6764 6938 Size: 1183 770 211 1143 773 967 1652 174 Cuts at: 6938 65 Size: 1396 Fragments arranged by size: 1652 1396 1183 1143 967 773 770 211 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6913 7100 1700 Size: 110 5103 187 2869 Fragments arranged by size: 5103 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3540 96 Size: 3444 4825 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8269 BssSI C'ACGA_G Cuts at: 4032 6329 7341 4032 Size: 2297 1012 4960 Fragments arranged by size: 4960 2297 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5102 794 Size: 4308 3961 BstBI TT'CG_AA Cuts at: 5322 7073 5322 Size: 1751 6518 BstEII G'GTnAC_C Cuts at: 1184 3791 4422 1184 Size: 2607 631 5031 Fragments arranged by size: 5031 2607 631 BstYI r'GATC_y Cuts at: 1972 2231 2490 2612 2809 3826 4312 4601 4655 Size: 259 259 122 197 1017 486 289 54 Cuts at: 4655 4715 5488 5599 5966 6351 6368 7820 7906 Size: 60 773 111 367 385 17 1452 86 Cuts at: 7906 7918 8264 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1017 773 486 385 367 346 289 259 259 197 122 111 86 60 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2637 4192 4914 5173 513 Size: 552 1572 1555 722 259 3609 Fragments arranged by size: 3609 1572 1555 722 552 259 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7530 Fragments arranged by size: 7530 683 56 BtsI GCAGTG_nn' Cuts at: 5204 5785 6579 6599 5204 Size: 581 794 20 6874 Fragments arranged by size: 6874 794 581 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8269 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8234 DraI TTT'AAA Cuts at: 5573 5573 Size: 8269 DraIII CAC_nnn'GTG Cuts at: 4430 4430 Size: 8269 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4305 7276 809 Size: 507 2989 2971 1802 Fragments arranged by size: 2989 2971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3151 4911 5374 Size: 54 1425 50 275 18 1321 1760 463 Cuts at: 5374 5716 6605 8234 8 Size: 342 889 1629 43 Fragments arranged by size: 1760 1629 1425 1321 889 463 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5716 1537 Size: 275 3904 4090 Fragments arranged by size: 4090 3904 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2403 3159 3979 4147 5319 5509 6199 1353 Size: 1050 756 820 168 1172 190 690 3423 Fragments arranged by size: 3423 1172 1050 820 756 690 190 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6856 7230 7376 387 Size: 1064 138 5267 374 146 1280 Fragments arranged by size: 5267 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2520 3665 3957 4109 4536 4636 4776 Size: 393 888 1145 292 152 427 100 140 Cuts at: 4776 5171 6332 6929 7716 7984 1239 Size: 395 1161 597 787 268 1524 Fragments arranged by size: 1524 1161 1145 888 787 597 427 395 393 292 268 152 140 100 EcoNI CCTnn'n_nnAGG Cuts at: 3236 4741 3236 Size: 1505 6764 EcoO109I rG'GnC_Cy Cuts at: 4476 4476 Size: 8269 EcoRI G'AATT_C Cuts at: 4055 5578 5956 4055 Size: 1523 378 6368 Fragments arranged by size: 6368 1523 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3599 3879 5535 3599 Size: 280 1656 6333 Fragments arranged by size: 6333 1656 280 FspI TGC'GCA Cuts at: 6775 6775 Size: 8269 HaeII r_GCGC'y Cuts at: 1678 7416 1678 Size: 5738 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 2402 2434 2585 Size: 32 135 32 671 32 127 32 151 Cuts at: 2585 2617 2801 2833 4515 4547 4735 4767 5375 Size: 32 184 32 1682 32 188 32 608 Cuts at: 5375 5407 6910 6942 6984 7016 1373 Size: 32 1503 32 42 32 2626 Fragments arranged by size: 2626 1682 1503 671 608 188 184 151 135 127 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5613 5859 669 Size: 988 281 3675 246 3079 Fragments arranged by size: 3675 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8269 HpaI GTT'AAC Cuts at: 5859 5859 Size: 8269 KpnI G_GTAC'C Cuts at: 5594 5594 Size: 8269 MfeI C'AATT_G Cuts at: 5868 5868 Size: 8269 MluI A'CGCG_T Cuts at: 8145 8145 Size: 8269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 3081 4430 4827 5516 7383 7567 1305 Size: 1035 741 1349 397 689 1867 184 2007 Fragments arranged by size: 2007 1867 1349 1035 741 689 397 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3153 10 Size: 54 1425 343 1321 5126 Fragments arranged by size: 5126 1425 1321 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 2535 6227 6586 6745 518 Size: 648 889 480 3692 359 159 2042 Fragments arranged by size: 3692 2042 889 648 480 359 159 MspA1I CmG'CkG Cuts at: 1606 5149 5685 6362 7510 7755 1606 Size: 3543 536 677 1148 245 2120 Fragments arranged by size: 3543 2120 1148 677 536 245 NcoI C'CATG_G Cuts at: 513 1065 2637 4192 513 Size: 552 1572 1555 4590 Fragments arranged by size: 4590 1572 1555 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8269 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8269 NotI GC'GGCC_GC Cuts at: 5716 5716 Size: 8269 NspI r_CATG'y Cuts at: 1132 1229 3638 5627 7172 1132 Size: 97 2409 1989 1545 2229 Fragments arranged by size: 2409 2229 1989 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8269 PciI A'CATG_T Cuts at: 7168 7168 Size: 8269 PflMI CCAn_nnn'nTGG Cuts at: 1108 3160 5092 1108 Size: 2052 1932 4285 Fragments arranged by size: 4285 2052 1932 PfoI T'CCnGG_A Cuts at: 2570 3718 5049 2570 Size: 1148 1331 5790 Fragments arranged by size: 5790 1331 1148 PmeI GTTT'AAAC Cuts at: 5573 5573 Size: 8269 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6338 6370 7851 7883 6338 Size: 32 1481 32 6724 Fragments arranged by size: 6724 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4476 4476 Size: 8269 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8269 PsiI TTA'TAA Cuts at: 4500 5839 4500 Size: 1339 6930 PspOMI G'GGCC_C Cuts at: 5169 5169 Size: 8269 PstI C_TGCA'G Cuts at: 830 5621 830 Size: 4791 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8269 PvuII CAG'CTG Cuts at: 5149 5149 Size: 8269 SalI G'TCGA_C Cuts at: 1936 5611 1936 Size: 3675 4594 SanDI GG'GwC_CC Cuts at: 4476 4476 Size: 8269 SapI GCTCTTCn'nnn_ Cuts at: 5509 5509 Size: 8269 SbfI CC_TGCA'GG Cuts at: 5621 5621 Size: 8269 ScaI AGT'ACT Cuts at: 4789 6517 8229 4789 Size: 1728 1712 4829 Fragments arranged by size: 4829 1728 1712 SexAI A'CCwGG_T Cuts at: 3113 3478 5191 3113 Size: 365 1713 6191 Fragments arranged by size: 6191 1713 365 SfcI C'TryA_G Cuts at: 826 1045 3462 4959 4981 5617 6752 7433 7624 Size: 219 2417 1497 22 636 1135 681 191 Cuts at: 7624 826 Size: 1471 Fragments arranged by size: 2417 1497 1471 1135 681 636 219 191 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8269 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8269 SmaI CCC'GGG Cuts at: 1872 5596 1872 Size: 3724 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2673 2770 3351 4631 6373 7274 Size: 197 926 730 97 581 1280 1742 901 Cuts at: 7274 7536 820 Size: 262 1553 Fragments arranged by size: 1742 1553 1280 926 901 730 581 262 197 97 SnaBI TAC'GTA Cuts at: 493 6060 493 Size: 5567 2702 SpeI A'CTAG_T Cuts at: 152 3080 152 Size: 2928 5341 SphI G_CATG'C Cuts at: 1132 5627 1132 Size: 4495 3774 SspI AAT'ATT Cuts at: 5 52 2195 6193 5 Size: 47 2143 3998 2081 Fragments arranged by size: 3998 2143 2081 47 StuI AGG'CCT Cuts at: 1517 3598 4908 1517 Size: 2081 1310 4878 Fragments arranged by size: 4878 2081 1310 StyI C'CwwG_G Cuts at: 513 1065 2637 3854 4192 5059 5496 5708 513 Size: 552 1572 1217 338 867 437 212 3074 Fragments arranged by size: 3074 1572 1217 867 552 437 338 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4441 5099 6296 6313 6466 6651 7096 Size: 1255 2861 658 1197 17 153 185 445 Cuts at: 7096 325 Size: 1498 Fragments arranged by size: 2861 1498 1255 1197 658 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3141 3540 4787 Size: 274 80 33 51 1443 1164 399 1247 Cuts at: 4787 4895 6515 8227 96 Size: 108 1620 1712 138 Fragments arranged by size: 1712 1620 1443 1247 1164 399 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4416 4448 4651 4683 8229 8261 4416 Size: 32 203 32 3546 32 4424 Fragments arranged by size: 4424 3546 203 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8269 XbaI T'CTAG_A Cuts at: 3553 5605 3553 Size: 2052 6217 XhoI C'TCGA_G Cuts at: 1943 2673 3351 1943 Size: 730 678 6861 Fragments arranged by size: 6861 730 678 XmaI C'CCGG_G Cuts at: 1870 5594 1870 Size: 3724 4545 XmnI GAAnn'nnTTC Cuts at: 5518 6398 5518 Size: 880 7389 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6077 276 Size: 53 83 186 936 4543 2468 Fragments arranged by size: 4543 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI