Restriction map for
pFN21AA0751
(Linear) (Six-base) MAPSORT of: KIAA0751.seq Check: 9589 from: 1 to: 3564
KIAA0751.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2736 3105 3564
Size: 2736 369 459
Fragments arranged by size:
2736 459 369
AccI GT'mk_AC
Cuts at: 0 2953 3564
Size: 2953 611
AclI AA'CG_TT
Cuts at: 0 2839 3564
Size: 2839 725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 2104 3564
Size: 515 1589 1460
Fragments arranged by size:
1589 1460 515
AflIII A'CryG_T
Cuts at: 0 1210 3564
Size: 1210 2354
AhdI GACnn_n'nnGTC
Cuts at: 0 2524 3564
Size: 2524 1040
AlwNI CAG_nnn'CTG
Cuts at: 0 3087 3564
Size: 3087 477
ApaI G_GGCC'C
Cuts at: 0 3168 3564
Size: 3168 396
ApoI r'AATT_y
Cuts at: 0 435 1868 1984 2050 3564
Size: 435 1433 116 66 1514
Fragments arranged by size:
1514 1433 435 116 66
AseI AT'TA_AT
Cuts at: 0 1795 2698 3564
Size: 1795 903 866
Fragments arranged by size:
1795 903 866
AvaI C'yCGr_G
Cuts at: 0 503 668 1346 3564
Size: 503 165 678 2218
Fragments arranged by size:
2218 678 503 165
BamHI G'GATC_C
Cuts at: 0 485 3564
Size: 485 3079
BanII G_rGCy'C
Cuts at: 0 2200 3168 3564
Size: 2200 968 396
Fragments arranged by size:
2200 968 396
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 1509 2184 3564
Size: 287 1222 675 1380
Fragments arranged by size:
1380 1222 675 287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1076 1110 3564
Size: 1076 34 2454
Fragments arranged by size:
2454 1076 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 204 3564
Size: 204 3360
BclI T'GATC_A
Cuts at: 0 288 3564
Size: 288 3276
BglII A'GATC_T
Cuts at: 0 226 607 1821 2307 2710 3564
Size: 226 381 1214 486 403 854
Fragments arranged by size:
1214 854 486 403 381 226
BlpI GC'TnA_GC
Cuts at: 0 3065 3564
Size: 3065 499
Bme1580I G_kGCm'C
Cuts at: 0 3168 3564
Size: 3168 396
BmrI ACTGGGnnnn_n'
Cuts at: 0 2417 2765 3564
Size: 2417 348 799
Fragments arranged by size:
2417 799 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1660 1952 2531 2631 2771 3166 3564
Size: 1660 292 579 100 140 395 398
Fragments arranged by size:
1660 579 398 395 292 140 100
Bpu10I CC'TnA_GC
Cuts at: 0 580 677 3564
Size: 580 97 2887
Fragments arranged by size:
2887 580 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 2647 3564
Size: 786 1861 917
Fragments arranged by size:
1861 917 786
BsaI GGTCTCn'nnnn_
Cuts at: 0 891 2381 2747 3564
Size: 891 1490 366 817
Fragments arranged by size:
1490 891 817 366
BsaAI yAC'GTr
Cuts at: 0 1211 3564
Size: 1211 2353
BsaBI GATnn'nnATC
Cuts at: 0 231 612 1289 2601 3564
Size: 231 381 677 1312 963
Fragments arranged by size:
1312 963 677 381 231
BsaHI Gr'CG_yC
Cuts at: 0 3078 3564
Size: 3078 486
BsaWI w'CCGG_w
Cuts at: 0 2485 3564
Size: 2485 1079
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2606 2636 3480 3510 3564
Size: 2606 30 844 30 54
Fragments arranged by size:
2606 844 54 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 750 985 1249 2369 2423 3488 3564
Size: 750 235 264 1120 54 1065 76
Fragments arranged by size:
1120 1065 750 264 235 76 54
BsiHKAI G_wGCw'C
Cuts at: 0 510 2878 3564
Size: 510 2368 686
Fragments arranged by size:
2368 686 510
BsmI GAATG_Cn'
Cuts at: 0 330 2003 3564
Size: 330 1673 1561
Fragments arranged by size:
1673 1561 330
Bsp1286I G_dGCh'C
Cuts at: 0 510 2200 2878 3168 3564
Size: 510 1690 678 290 396
Fragments arranged by size:
1690 678 510 396 290
BspHI T'CATG_A
Cuts at: 0 2678 3564
Size: 2678 886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2736 3105 3564
Size: 2736 369 459
Fragments arranged by size:
2736 459 369
BsrBI CCG'CTC
Cuts at: 0 2631 2645 3564
Size: 2631 14 919
Fragments arranged by size:
2631 919 14
BsrDI GCAATG_nn'
Cuts at: 0 13 224 1367 2140 3107 3564
Size: 13 211 1143 773 967 457
Fragments arranged by size:
1143 967 773 457 211 13
BsrGI T'GTAC_A
Cuts at: 0 1535 3564
Size: 1535 2029
BssSI C'ACGA_G
Cuts at: 0 2027 3564
Size: 2027 1537
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3097 3564
Size: 3097 467
BstBI TT'CG_AA
Cuts at: 0 3317 3564
Size: 3317 247
BstEII G'GTnAC_C
Cuts at: 0 1786 2417 3564
Size: 1786 631 1147
Fragments arranged by size:
1786 1147 631
BstYI r'GATC_y
Cuts at: 0 226 485 607 804 1821 2307 2596 2650
Size: 226 259 122 197 1017 486 289 54
Cuts at: 2650 2710 3483 3564
Size: 60 773 81
Fragments arranged by size:
1017 773 486 289 259 226 197 122
81 60 54
BtgI C'CryG_G
Cuts at: 0 632 2187 2909 3168 3564
Size: 632 1555 722 259 396
Fragments arranged by size:
1555 722 632 396 259
BtsI GCAGTG_nn'
Cuts at: 0 3199 3564
Size: 3199 365
DraIII CAC_nnn'GTG
Cuts at: 0 2425 3564
Size: 2425 1139
DrdI GACnn_nn'nnGTC
Cuts at: 0 2300 3564
Size: 2300 1264
EaeI y'GGCC_r
Cuts at: 0 1146 2906 3369 3564
Size: 1146 1760 463 195
Fragments arranged by size:
1760 1146 463 195
EarI CTCTTCn'nnn_
Cuts at: 0 398 1154 1974 2142 3314 3504 3564
Size: 398 756 820 168 1172 190 60
Fragments arranged by size:
1172 820 756 398 190 168 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1660 1952 2104 2531 2631 2771 3166
Size: 515 1145 292 152 427 100 140 395
Cuts at: 3166 3564
Size: 398
Fragments arranged by size:
1145 515 427 398 395 292 152 140
100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1231 2736 3564
Size: 1231 1505 828
Fragments arranged by size:
1505 1231 828
EcoO109I rG'GnC_Cy
Cuts at: 0 2471 3564
Size: 2471 1093
EcoRI G'AATT_C
Cuts at: 0 2050 3564
Size: 2050 1514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1594 1874 3530 3564
Size: 1594 280 1656 34
Fragments arranged by size:
1656 1594 280 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 397 429 580 612 796 828
Size: 238 32 127 32 151 32 184 32
Cuts at: 828 2510 2542 2730 2762 3370 3402 3564
Size: 1682 32 188 32 608 32 162
Fragments arranged by size:
1682 608 238 188 184 162 151 127
32 32 32 32 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 1076 2425 2822 3511 3564
Size: 335 741 1349 397 689 53
Fragments arranged by size:
1349 741 689 397 335 53
MscI TGG'CCA
Cuts at: 0 1148 3564
Size: 1148 2416
MslI CAynn'nnrTG
Cuts at: 0 50 530 3564
Size: 50 480 3034
Fragments arranged by size:
3034 480 50
MspA1I CmG'CkG
Cuts at: 0 3144 3564
Size: 3144 420
NcoI C'CATG_G
Cuts at: 0 632 2187 3564
Size: 632 1555 1377
Fragments arranged by size:
1555 1377 632
NspI r_CATG'y
Cuts at: 0 1633 3564
Size: 1633 1931
PflMI CCAn_nnn'nTGG
Cuts at: 0 1155 3087 3564
Size: 1155 1932 477
Fragments arranged by size:
1932 1155 477
PfoI T'CCnGG_A
Cuts at: 0 565 1713 3044 3564
Size: 565 1148 1331 520
Fragments arranged by size:
1331 1148 565 520
PpuMI rG'GwC_Cy
Cuts at: 0 2471 3564
Size: 2471 1093
PsiI TTA'TAA
Cuts at: 0 2495 3564
Size: 2495 1069
PspOMI G'GGCC_C
Cuts at: 0 3164 3564
Size: 3164 400
PvuII CAG'CTG
Cuts at: 0 3144 3564
Size: 3144 420
SanDI GG'GwC_CC
Cuts at: 0 2471 3564
Size: 2471 1093
SapI GCTCTTCn'nnn_
Cuts at: 0 3504 3564
Size: 3504 60
ScaI AGT'ACT
Cuts at: 0 2784 3564
Size: 2784 780
SexAI A'CCwGG_T
Cuts at: 0 1108 1473 3186 3564
Size: 1108 365 1713 378
Fragments arranged by size:
1713 1108 378 365
SfcI C'TryA_G
Cuts at: 0 1457 2954 2976 3564
Size: 1457 1497 22 588
Fragments arranged by size:
1497 1457 588 22
SmlI C'TyrA_G
Cuts at: 0 668 765 1346 2626 3564
Size: 668 97 581 1280 938
Fragments arranged by size:
1280 938 668 581 97
SpeI A'CTAG_T
Cuts at: 0 1075 3564
Size: 1075 2489
SspI AAT'ATT
Cuts at: 0 190 3564
Size: 190 3374
StuI AGG'CCT
Cuts at: 0 1593 2903 3564
Size: 1593 1310 661
Fragments arranged by size:
1593 1310 661
StyI C'CwwG_G
Cuts at: 0 632 1849 2187 3054 3491 3564
Size: 632 1217 338 867 437 73
Fragments arranged by size:
1217 867 632 437 338 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2436 3094 3564
Size: 2436 658 470
Fragments arranged by size:
2436 658 470
TatI w'GTAC_w
Cuts at: 0 1136 1535 2782 2890 3564
Size: 1136 399 1247 108 674
Fragments arranged by size:
1247 1136 674 399 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2411 2443 2646 2678 3564
Size: 2411 32 203 32 886
Fragments arranged by size:
2411 886 203 32 32
XbaI T'CTAG_A
Cuts at: 0 1548 3564
Size: 1548 2016
XhoI C'TCGA_G
Cuts at: 0 668 1346 3564
Size: 668 678 2218
Fragments arranged by size:
2218 678 668
XmnI GAAnn'nnTTC
Cuts at: 0 3513 3564
Size: 3513 51
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AhdI AlwNI ApaI
ApoI AseI AvaI BamHI BanII BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtsI DraIII DrdI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
MmeI MscI MslI MspA1I NcoI NspI PflMI PfoI
PpuMI PsiI PspOMI PvuII SanDI SapI ScaI SexAI
SfcI SmlI SpeI SspI StuI StyI TaqII TatI
TstI XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AleI AlfI AloI
ApaLI AscI AsiSI AvrII BaeI BanI BbeI BbvCI
BfrBI BglI BmgBI BmtI BplI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BsrFI BssHII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI EagI EciI EcoICRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SwaI Tth111I XcmI XmaI ZraI