(Circular) (Six-base) MAPSORT of: pFN21AA0720.seq Check: 7437 from: 1 to: 7891 pFN21AA0720 7891 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4797 4797 Size: 7891 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5701 278 Size: 53 83 186 936 4165 2468 Fragments arranged by size: 4165 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2973 5234 1937 Size: 1036 2261 4594 Fragments arranged by size: 4594 2261 1036 Acc65I G'GTAC_C Cuts at: 5212 5212 Size: 7891 AclI AA'CG_TT Cuts at: 1563 6018 6391 1563 Size: 4455 373 3063 Fragments arranged by size: 4455 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2905 3617 3878 4344 5954 7338 7606 1632 Size: 1273 712 261 466 1610 1384 268 1917 Fragments arranged by size: 1917 1610 1384 1273 712 466 268 261 AfeI AGC'GCT Cuts at: 3414 3731 3414 Size: 317 7574 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7694 AflIII A'CryG_T Cuts at: 1203 2235 2787 3750 4158 6790 7767 1203 Size: 1032 552 963 408 2632 977 1327 Fragments arranged by size: 2632 1327 1032 977 963 552 408 AgeI A'CCGG_T Cuts at: 6722 6722 Size: 7891 AhdI GACnn_n'nnGTC Cuts at: 2665 4105 4480 6620 2665 Size: 1440 375 2140 3936 Fragments arranged by size: 3936 2140 1440 375 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7891 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7891 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3305 3337 3305 Size: 32 7859 AlwNI CAG_nnn'CTG Cuts at: 2113 3595 3784 3928 4273 4282 4828 4838 5634 Size: 1482 189 144 345 9 546 10 796 Cuts at: 5634 7206 2113 Size: 1572 2798 Fragments arranged by size: 2798 1572 1482 796 546 345 189 144 10 9 ApaI G_GGCC'C Cuts at: 2130 2709 4702 4947 4969 2130 Size: 579 1993 245 22 5052 Fragments arranged by size: 5052 1993 579 245 22 ApaLI G'TGCA_C Cuts at: 4242 5948 7104 4242 Size: 1706 1156 5029 Fragments arranged by size: 5029 1706 1156 ApoI r'AATT_y Cuts at: 1493 2411 3863 5200 5426 5578 7687 1493 Size: 918 1452 1337 226 152 2109 1697 Fragments arranged by size: 2109 1697 1452 1337 918 226 152 AseI AT'TA_AT Cuts at: 160 1032 6445 160 Size: 872 5413 1606 Fragments arranged by size: 5413 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7891 AvaI C'yCGr_G Cuts at: 1870 1943 2819 3039 3425 4490 4695 5056 5216 Size: 73 876 220 386 1065 205 361 160 Cuts at: 5216 1870 Size: 4545 Fragments arranged by size: 4545 1065 876 386 361 220 205 160 73 BamHI G'GATC_C Cuts at: 5221 5588 5221 Size: 367 7524 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3379 3899 4631 4841 5212 Size: 325 217 627 1592 520 732 210 371 Cuts at: 5212 6667 618 Size: 1455 1842 Fragments arranged by size: 1842 1592 1455 732 627 520 371 325 217 210 BanII G_rGCy'C Cuts at: 1391 2021 2130 2306 2709 2832 3141 4254 4702 Size: 630 109 176 403 123 309 1113 448 Cuts at: 4702 4887 4928 4947 4969 5088 5210 1391 Size: 185 41 19 22 119 122 4072 Fragments arranged by size: 4072 1113 630 448 403 309 185 176 123 122 119 109 41 22 19 BbeI G_GCGC'C Cuts at: 3383 3903 3383 Size: 520 7371 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3093 3680 928 Size: 971 1194 587 5139 Fragments arranged by size: 5139 1194 971 587 BbvCI CC'TCA_GC Cuts at: 3668 3668 Size: 7891 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6082 6116 1903 Size: 34 4145 34 3678 Fragments arranged by size: 4145 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5783 6993 5783 Size: 1210 6681 BclI T'GATC_A Cuts at: 1548 3243 1548 Size: 1695 6196 BfrBI ATG'CAT Cuts at: 2176 2176 Size: 7891 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3043 3698 6502 Size: 107 122 71 631 605 1371 655 2804 Cuts at: 6502 136 Size: 1525 Fragments arranged by size: 2804 1525 1371 655 631 605 122 107 71 BglII A'GATC_T Cuts at: 7886 7886 Size: 7891 BlpI GC'TnA_GC Cuts at: 3602 5308 3602 Size: 1706 6185 Bme1580I G_kGCm'C Cuts at: 1790 2130 2709 4246 4702 4947 4969 5952 7108 Size: 340 579 1537 456 245 22 983 1156 Cuts at: 7108 1790 Size: 2573 Fragments arranged by size: 2573 1537 1156 983 579 456 340 245 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7891 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2480 4513 5622 Size: 416 53 83 399 24 1063 2033 1109 Cuts at: 5622 6580 442 Size: 958 1753 Fragments arranged by size: 2033 1753 1109 1063 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3372 1056 Size: 2316 5575 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2320 2320 Size: 7891 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2646 2738 2827 3011 3140 3410 3953 4805 Size: 1407 92 89 184 129 270 543 852 Cuts at: 4805 6551 1239 Size: 1746 2579 Fragments arranged by size: 2579 1746 1407 852 543 270 184 129 92 89 Bpu10I CC'TnA_GC Cuts at: 3668 5082 3668 Size: 1414 6477 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3641 4778 6016 6881 7179 3641 Size: 1137 1238 865 298 4353 Fragments arranged by size: 4353 1238 1137 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4436 4523 5040 5104 6554 882 Size: 617 2937 87 517 64 1450 2219 Fragments arranged by size: 2937 2219 1450 617 517 87 64 BsaAI yAC'GTr Cuts at: 493 1204 3564 3745 5682 493 Size: 711 2360 181 1937 2702 Fragments arranged by size: 2702 2360 1937 711 181 BsaBI GATnn'nnATC Cuts at: 7885 7885 Size: 7891 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2730 3380 3810 3900 Size: 53 83 186 936 1197 650 430 90 Cuts at: 3900 5698 6080 6715 275 Size: 1798 382 635 1451 Fragments arranged by size: 1798 1451 1197 936 650 635 430 382 186 90 83 53 BsaWI w'CCGG_w Cuts at: 2325 4591 6324 6722 6996 7143 7554 2325 Size: 2266 1733 398 274 147 411 2662 Fragments arranged by size: 2662 2266 1733 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3047 3062 3077 3092 3179 Size: 30 165 30 1644 15 15 15 87 Cuts at: 3179 3209 3305 3335 4589 4619 1178 Size: 30 96 30 1254 30 4450 Fragments arranged by size: 4450 1644 1254 165 96 87 30 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 2303 2306 2309 2312 2383 3086 3089 Size: 1114 3 3 3 3 71 703 3 Cuts at: 3089 3509 3512 3695 3926 4304 4307 4322 4331 Size: 420 3 183 231 378 3 15 9 Cuts at: 4331 4334 4337 4340 4343 4346 4349 4808 1186 Size: 3 3 3 3 3 3 459 4269 Fragments arranged by size: 4269 1114 703 459 420 378 231 183 71 15 9 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2449 2515 2614 2696 2858 2945 4194 4865 4940 Size: 66 99 82 162 87 1249 671 75 Cuts at: 4940 4946 4950 5106 5130 7094 7590 2449 Size: 6 4 156 24 1964 496 2750 Fragments arranged by size: 2750 1964 1249 671 496 162 156 99 87 82 75 66 24 6 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2099 2987 3191 3335 3854 1163 Size: 573 363 888 204 144 519 5200 Fragments arranged by size: 5200 888 573 519 363 204 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3792 4603 5341 6102 7130 1540 Size: 275 186 1791 811 738 761 1028 2301 Fragments arranged by size: 2301 1791 1028 811 761 738 275 186 BsiHKAI G_wGCw'C Cuts at: 2306 2336 3141 4246 4887 5952 6037 7108 2306 Size: 30 805 1105 641 1065 85 1071 3089 Fragments arranged by size: 3089 1105 1071 1065 805 641 85 30 BsmI GAATG_Cn' Cuts at: 2402 5402 5495 2402 Size: 3000 93 4798 Fragments arranged by size: 4798 3000 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3908 4748 3908 Size: 840 7051 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2130 2306 2336 2709 2832 3141 Size: 399 231 109 176 30 373 123 309 Cuts at: 3141 4246 4254 4702 4887 4928 4947 4969 5088 Size: 1105 8 448 185 41 19 22 119 Cuts at: 5088 5210 5952 6037 7108 1391 Size: 122 742 85 1071 2174 Fragments arranged by size: 2174 1105 1071 742 448 399 373 309 231 185 176 123 122 119 109 85 41 30 22 19 8 BspEI T'CCGG_A Cuts at: 7554 7554 Size: 7891 BspHI T'CATG_A Cuts at: 5780 7510 5780 Size: 1730 6161 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3285 4797 5026 5058 5246 844 Size: 2441 1512 229 32 188 3489 Fragments arranged by size: 3489 2441 1512 229 188 32 BsrBI CCG'CTC Cuts at: 1394 1823 3775 4605 5778 1394 Size: 429 1952 830 1173 3507 Fragments arranged by size: 3507 1952 1173 830 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2508 6386 6560 65 Size: 1183 1260 3878 174 1396 Fragments arranged by size: 3878 1396 1260 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3382 6535 6722 1700 Size: 110 1572 3153 187 2869 Fragments arranged by size: 3153 2869 1572 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7891 BssHII G'CGCG_C Cuts at: 1925 3688 1925 Size: 1763 6128 BssSI C'ACGA_G Cuts at: 4067 4713 5951 6963 4067 Size: 646 1238 1012 4995 Fragments arranged by size: 4995 1238 1012 646 BstAPI GCAn_nnn'nTGC Cuts at: 794 2397 3928 4237 4273 794 Size: 1603 1531 309 36 4412 Fragments arranged by size: 4412 1603 1531 309 36 BstBI TT'CG_AA Cuts at: 6695 6695 Size: 7891 BstEII G'GTnAC_C Cuts at: 1184 4012 1184 Size: 2828 5063 BstXI CCAn_nnnn'nTGG Cuts at: 4172 4459 4172 Size: 287 7604 BstYI r'GATC_y Cuts at: 1972 4000 5221 5588 5973 5990 7442 7528 7540 Size: 2028 1221 367 385 17 1452 86 12 Cuts at: 7540 7886 1972 Size: 346 1977 Fragments arranged by size: 2028 1977 1452 1221 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3113 3944 4932 3113 Size: 831 988 6072 Fragments arranged by size: 6072 988 831 BtgI C'CryG_G Cuts at: 513 1065 2004 3723 3792 4189 4437 4452 4614 Size: 552 939 1719 69 397 248 15 162 Cuts at: 4614 4846 513 Size: 232 3558 Fragments arranged by size: 3558 1719 939 552 397 248 232 162 69 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7152 Fragments arranged by size: 7152 683 56 BtsI GCAGTG_nn' Cuts at: 2280 2875 3179 4029 4545 5175 5407 6201 6221 Size: 595 304 850 516 630 232 794 20 Cuts at: 6221 2280 Size: 3950 Fragments arranged by size: 3950 850 794 630 595 516 304 232 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7891 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7856 DraI TTT'AAA Cuts at: 5195 5195 Size: 7891 DraIII CAC_nnn'GTG Cuts at: 3745 3745 Size: 7891 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2043 6898 809 Size: 507 727 4855 1802 Fragments arranged by size: 4855 1802 727 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2690 2770 Size: 54 1425 50 275 18 249 611 80 Cuts at: 2770 2910 2962 3615 3789 4174 4449 4600 5007 Size: 140 52 653 174 385 275 151 407 Cuts at: 5007 5338 6227 7856 8 Size: 331 889 1629 43 Fragments arranged by size: 1629 1425 889 653 611 407 385 331 275 275 249 174 151 140 80 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3789 4600 5338 1537 Size: 275 1977 811 738 4090 Fragments arranged by size: 4090 1977 811 738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2324 3023 3470 3551 3998 4281 4308 5030 Size: 971 699 447 81 447 283 27 722 Cuts at: 5030 5821 1353 Size: 791 3423 Fragments arranged by size: 3423 971 791 722 699 447 447 283 81 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3326 3584 4736 6478 6852 6998 Size: 1064 138 1737 258 1152 1742 374 146 Cuts at: 6998 387 Size: 1280 Fragments arranged by size: 1742 1737 1280 1152 1064 374 258 146 138 EcoICRI GAG'CTC Cuts at: 2304 3139 4885 2304 Size: 835 1746 5310 Fragments arranged by size: 5310 1746 835 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2646 2738 2827 2905 3011 3140 3410 Size: 393 1014 92 89 78 106 129 270 Cuts at: 3410 3617 3878 3953 4344 4805 5954 6551 7338 Size: 207 261 75 391 461 1149 597 787 Cuts at: 7338 7606 1239 Size: 268 1524 Fragments arranged by size: 1524 1149 1014 787 597 461 393 391 270 268 261 207 129 106 92 89 78 75 EcoNI CCTnn'n_nnAGG Cuts at: 2141 2804 3112 3226 4286 5068 2141 Size: 663 308 114 1060 782 4964 Fragments arranged by size: 4964 1060 782 663 308 114 EcoO109I rG'GnC_Cy Cuts at: 2126 2127 2706 4099 4107 4152 4699 4965 4966 Size: 1 579 1393 8 45 547 266 1 Cuts at: 4966 5061 2126 Size: 95 4956 Fragments arranged by size: 4956 1393 579 547 266 95 45 8 1 1 EcoRI G'AATT_C Cuts at: 5200 5578 5200 Size: 378 7513 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 7891 FspI TGC'GCA Cuts at: 6397 6397 Size: 7891 HaeII r_GCGC'y Cuts at: 1678 2958 3319 3383 3416 3733 3890 3903 7038 Size: 1280 361 64 33 317 157 13 3135 Cuts at: 7038 1678 Size: 2531 Fragments arranged by size: 3135 2531 1280 361 317 157 64 33 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2651 2683 3047 3079 4091 Size: 32 135 32 1079 32 364 32 1012 Cuts at: 4091 4123 4466 4498 4517 4549 4589 4621 5039 Size: 32 343 32 19 32 40 32 418 Cuts at: 5039 5071 6532 6564 6606 6638 1373 Size: 32 1461 32 42 32 2626 Fragments arranged by size: 2626 1461 1079 1012 418 364 343 135 42 40 32 32 32 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 5235 5481 669 Size: 988 281 3297 246 3079 Fragments arranged by size: 3297 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7891 HpaI GTT'AAC Cuts at: 5481 5481 Size: 7891 KasI G'GCGC_C Cuts at: 3379 3899 3379 Size: 520 7371 KpnI G_GTAC'C Cuts at: 5216 5216 Size: 7891 MfeI C'AATT_G Cuts at: 5490 5490 Size: 7891 MluI A'CGCG_T Cuts at: 3750 7767 3750 Size: 4017 3874 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2571 2772 7005 7189 1305 Size: 1266 201 4233 184 2007 Fragments arranged by size: 4233 2007 1266 201 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3617 4176 4451 10 Size: 54 1425 343 1785 559 275 3450 Fragments arranged by size: 3450 1785 1425 559 343 275 54 MslI CAynn'nnrTG Cuts at: 518 1166 2181 2379 4457 5849 6208 6367 518 Size: 648 1015 198 2078 1392 359 159 2042 Fragments arranged by size: 2078 2042 1392 1015 648 359 198 159 MspA1I CmG'CkG Cuts at: 1606 2024 2113 2696 2847 2862 2895 2925 2989 Size: 418 89 583 151 15 33 30 64 Cuts at: 2989 3129 3349 3631 3649 3766 3781 3925 4228 Size: 140 220 282 18 117 15 144 303 Cuts at: 4228 4237 4364 4398 4503 4768 4825 4950 4983 Size: 9 127 34 105 265 57 125 33 Cuts at: 4983 5307 5984 7132 7377 1606 Size: 324 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 583 418 324 303 282 265 245 220 151 144 140 127 125 117 105 89 64 57 34 33 33 30 18 15 15 9 NarI GG'CG_CC Cuts at: 3380 3900 3380 Size: 520 7371 NcoI C'CATG_G Cuts at: 513 1065 2004 4452 4614 4846 513 Size: 552 939 2448 162 232 3558 Fragments arranged by size: 3558 2448 939 552 232 162 NdeI CA'TA_TG Cuts at: 387 387 Size: 7891 NheI G'CTAG_C Cuts at: 1052 3368 1052 Size: 2316 5575 NotI GC'GGCC_GC Cuts at: 5338 5338 Size: 7891 NsiI A_TGCA'T Cuts at: 2178 2178 Size: 7891 NspI r_CATG'y Cuts at: 1132 1229 2192 2791 3520 3613 3760 5249 6794 Size: 97 963 599 729 93 147 1489 1545 Cuts at: 6794 1132 Size: 2229 Fragments arranged by size: 2229 1545 1489 963 729 599 147 97 93 PasI CC'CwG_GG Cuts at: 1348 4103 4585 5070 1348 Size: 2755 482 485 4169 Fragments arranged by size: 4169 2755 485 482 PciI A'CATG_T Cuts at: 2787 6790 2787 Size: 4003 3888 PflMI CCAn_nnn'nTGG Cuts at: 1108 4828 1108 Size: 3720 4171 PfoI T'CCnGG_A Cuts at: 3333 3333 Size: 7891 PmeI GTTT'AAAC Cuts at: 5195 5195 Size: 7891 PmlI CAC'GTG Cuts at: 3564 3745 3564 Size: 181 7710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3305 3337 5960 5992 7473 7505 3305 Size: 32 2623 32 1481 32 3691 Fragments arranged by size: 3691 2623 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4099 4107 4099 Size: 8 7883 PshAI GACnn'nnGTC Cuts at: 1613 4046 4503 1613 Size: 2433 457 5001 Fragments arranged by size: 5001 2433 457 PsiI TTA'TAA Cuts at: 5461 5461 Size: 7891 PspOMI G'GGCC_C Cuts at: 2126 2705 4698 4943 4965 2126 Size: 579 1993 245 22 5052 Fragments arranged by size: 5052 1993 579 245 22 PstI C_TGCA'G Cuts at: 830 4278 5023 5243 830 Size: 3448 745 220 3478 Fragments arranged by size: 3478 3448 745 220 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7891 PvuII CAG'CTG Cuts at: 2113 2696 2847 2895 2989 3129 3349 3925 4228 Size: 583 151 48 94 140 220 576 303 Cuts at: 4228 4237 4364 4768 4825 4950 2113 Size: 9 127 404 57 125 5054 Fragments arranged by size: 5054 583 576 404 303 220 151 140 127 125 94 57 48 9 SacI G_AGCT'C Cuts at: 2306 3141 4887 2306 Size: 835 1746 5310 Fragments arranged by size: 5310 1746 835 SalI G'TCGA_C Cuts at: 1936 5233 1936 Size: 3297 4594 SanDI GG'GwC_CC Cuts at: 4099 4099 Size: 7891 SapI GCTCTTCn'nnn_ Cuts at: 2324 3470 2324 Size: 1146 6745 SbfI CC_TGCA'GG Cuts at: 5023 5243 5023 Size: 220 7671 ScaI AGT'ACT Cuts at: 6139 7851 6139 Size: 1712 6179 SfcI C'TryA_G Cuts at: 826 1045 2578 3010 3419 4146 4274 5019 5239 Size: 219 1533 432 409 727 128 745 220 Cuts at: 5239 6374 7055 7246 826 Size: 1135 681 191 1471 Fragments arranged by size: 1533 1471 1135 745 727 681 432 409 220 219 191 128 SfoI GGC'GCC Cuts at: 3381 3901 3381 Size: 520 7371 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7891 SgrAI Cr'CCGG_yG Cuts at: 1700 3382 1700 Size: 1682 6209 SmaI CCC'GGG Cuts at: 1872 2821 3041 3427 5218 1872 Size: 949 220 386 1791 4545 Fragments arranged by size: 4545 1791 949 386 220 SmlI C'TyrA_G Cuts at: 820 1017 1943 3656 4793 5995 6896 7158 820 Size: 197 926 1713 1137 1202 901 262 1553 Fragments arranged by size: 1713 1553 1202 1137 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5682 493 Size: 5189 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7891 SphI G_CATG'C Cuts at: 1132 3760 5249 1132 Size: 2628 1489 3774 Fragments arranged by size: 3774 2628 1489 SspI AAT'ATT Cuts at: 5 52 5815 5 Size: 47 5763 2081 Fragments arranged by size: 5763 2081 47 StuI AGG'CCT Cuts at: 1517 2147 2340 2576 3218 4181 1517 Size: 630 193 236 642 963 5227 Fragments arranged by size: 5227 963 642 630 236 193 StyI C'CwwG_G Cuts at: 513 1065 2004 2082 3693 4452 4614 4846 5330 Size: 552 939 78 1611 759 162 232 484 Cuts at: 5330 513 Size: 3074 Fragments arranged by size: 3074 1611 939 759 552 484 232 162 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3594 3692 4186 4859 5918 5935 6088 Size: 1255 2014 98 494 673 1059 17 153 Cuts at: 6088 6273 6718 325 Size: 185 445 1498 Fragments arranged by size: 2014 1498 1255 1059 673 494 445 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3513 3981 6137 Size: 274 80 33 51 1443 1536 468 2156 Cuts at: 6137 7849 96 Size: 1712 138 Fragments arranged by size: 2156 1712 1536 1443 468 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3179 3211 5026 5058 7851 7883 3179 Size: 32 1815 32 2793 32 3187 Fragments arranged by size: 3187 2793 1815 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7891 XbaI T'CTAG_A Cuts at: 5227 5227 Size: 7891 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 4444 2272 Size: 2172 5719 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7891 XmaI C'CCGG_G Cuts at: 1870 2819 3039 3425 5216 1870 Size: 949 220 386 1791 4545 Fragments arranged by size: 4545 1791 949 386 220 XmnI GAAnn'nnTTC Cuts at: 6020 6020 Size: 7891 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5699 276 Size: 53 83 186 936 4165 2468 Fragments arranged by size: 4165 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI