Restriction map for pFN21AA0720
 (Linear) (Six-base) MAPSORT of: KIAA0720.seq  Check: 1531  from: 1  to: 3186



KIAA0720.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2792    3186 
   Size:       2792     394
 
AccI GT'mk_AC

Cuts at:      0     968    3186 
   Size:        968    2218
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     900    1612    1873    2339    3186 
   Size:        900     712     261     466     847

  Fragments arranged by size: 

                 900     847     712     466     261
 
AfeI AGC'GCT

Cuts at:      0    1409    1726    3186 
   Size:       1409     317    1460

  Fragments arranged by size: 

                1460    1409     317
 
AflIII A'CryG_T

Cuts at:      0     230     782    1745    2153    3186 
   Size:        230     552     963     408    1033

  Fragments arranged by size: 

                1033     963     552     408     230
 
AhdI GACnn_n'nnGTC

Cuts at:      0     660    2100    2475    3186 
   Size:        660    1440     375     711

  Fragments arranged by size: 

                1440     711     660     375
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1300    1332    3186 
   Size:       1300      32    1854

  Fragments arranged by size: 

                1854    1300      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     108    1590    1779    1923    2268    2277    2823    2833 
   Size:        108    1482     189     144     345       9     546      10

Cuts at:   2833    3186 
   Size:        353

  Fragments arranged by size: 

                1482     546     353     345     189     144     108      10
                   9
 
ApaI G_GGCC'C

Cuts at:      0     125     704    2697    2942    2964    3186 
   Size:        125     579    1993     245      22     222

  Fragments arranged by size: 

                1993     579     245     222     125      22
 
ApaLI G'TGCA_C

Cuts at:      0    2237    3186 
   Size:       2237     949
 
ApoI r'AATT_y

Cuts at:      0     406    1858    3186 
   Size:        406    1452    1328

  Fragments arranged by size: 

                1452    1328     406
 
AvaI C'yCGr_G

Cuts at:      0     814    1034    1420    2485    2690    3051    3186 
   Size:        814     220     386    1065     205     361     135

  Fragments arranged by size: 

                1065     814     386     361     220     205     135
 
BanI G'GyrC_C

Cuts at:      0    1374    1894    2626    2836    3186 
   Size:       1374     520     732     210     350

  Fragments arranged by size: 

                1374     732     520     350     210
 
BanII G_rGCy'C

Cuts at:      0      16     125     301     704     827    1136    2249    2697 
   Size:         16     109     176     403     123     309    1113     448

Cuts at:   2697    2882    2923    2942    2964    3083    3186 
   Size:        185      41      19      22     119     103

  Fragments arranged by size: 

                1113     448     403     309     185     176     123     119
                 109     103      41      22      19      16
 
BbeI G_GCGC'C

Cuts at:      0    1378    1898    3186 
   Size:       1378     520    1288

  Fragments arranged by size: 

                1378    1288     520
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1088    1675    3186 
   Size:       1088     587    1511

  Fragments arranged by size: 

                1511    1088     587
 
BbvCI CC'TCA_GC

Cuts at:      0    1663    3186 
   Size:       1663    1523
 
BclI T'GATC_A

Cuts at:      0    1238    3186 
   Size:       1238    1948
 
BfrBI ATG'CAT

Cuts at:      0     171    3186 
   Size:        171    3015
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1038    1693    3186 
   Size:       1038     655    1493

  Fragments arranged by size: 

                1493    1038     655
 
BlpI GC'TnA_GC

Cuts at:      0    1597    3186 
   Size:       1597    1589
 
Bme1580I G_kGCm'C

Cuts at:      0     125     704    2241    2697    2942    2964    3186 
   Size:        125     579    1537     456     245      22     222

  Fragments arranged by size: 

                1537     579     456     245     222     125      22
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     475    2508    3186 
   Size:        475    2033     678

  Fragments arranged by size: 

                2033     678     475
 
BmtI G_CTAG'C

Cuts at:      0    1367    3186 
   Size:       1367    1819
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     315    3186 
   Size:        315    2871
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     733     822    1006    1135    1405    1948    2800 
   Size:        641      92      89     184     129     270     543     852

Cuts at:   2800    3186 
   Size:        386

  Fragments arranged by size: 

                 852     641     543     386     270     184     129      92
                  89
 
Bpu10I CC'TnA_GC

Cuts at:      0    1663    3077    3186 
   Size:       1663    1414     109

  Fragments arranged by size: 

                1663    1414     109
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1636    2773    3186 
   Size:       1636    1137     413

  Fragments arranged by size: 

                1636    1137     413
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2431    2518    3035    3099    3186 
   Size:       2431      87     517      64      87

  Fragments arranged by size: 

                2431     517      87      87      64
 
BsaAI yAC'GTr

Cuts at:      0    1559    1740    3186 
   Size:       1559     181    1446

  Fragments arranged by size: 

                1559    1446     181
 
BsaHI Gr'CG_yC

Cuts at:      0     725    1375    1805    1895    3186 
   Size:        725     650     430      90    1291

  Fragments arranged by size: 

                1291     725     650     430      90
 
BsaWI w'CCGG_w

Cuts at:      0     320    2586    3186 
   Size:        320    2266     600

  Fragments arranged by size: 

                2266     600     320
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1042    1057    1072    1087    1174    1204    1300    1330 
   Size:       1042      15      15      15      87      30      96      30

Cuts at:   1330    2584    2614    3186 
   Size:       1254      30     572

  Fragments arranged by size: 

                1254    1042     572      96      87      30      30      30
                  15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     295     298     301     304     307     378    1081    1084 
   Size:        295       3       3       3       3      71     703       3

Cuts at:   1084    1504    1507    1690    1921    2299    2302    2317    2326 
   Size:        420       3     183     231     378       3      15       9

Cuts at:   2326    2329    2332    2335    2338    2341    2344    2803    3186 
   Size:          3       3       3       3       3       3     459     383

  Fragments arranged by size: 

                 703     459     420     383     378     295     231     183
                  71      15       9       3       3       3       3       3
                   3       3       3       3       3       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     444     510     609     691     853     940    2189    2860 
   Size:        444      66      99      82     162      87    1249     671

Cuts at:   2860    2935    2941    2945    3101    3125    3186 
   Size:         75       6       4     156      24      61

  Fragments arranged by size: 

                1249     671     444     162     156      99      87      82
                  75      66      61      24       6       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     982    1186    1330    1849    3186 
   Size:         94     888     204     144     519    1337

  Fragments arranged by size: 

                1337     888     519     204     144      94
 
BsiEI CG_ry'CG

Cuts at:      0    1787    2598    3186 
   Size:       1787     811     588

  Fragments arranged by size: 

                1787     811     588
 
BsiHKAI G_wGCw'C

Cuts at:      0     301     331    1136    2241    2882    3186 
   Size:        301      30     805    1105     641     304

  Fragments arranged by size: 

                1105     805     641     304     301      30
 
BsmI GAATG_Cn'

Cuts at:      0     397    3186 
   Size:        397    2789
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1903    2743    3186 
   Size:       1903     840     443

  Fragments arranged by size: 

                1903     840     443
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     125     301     331     704     827    1136    2241 
   Size:         16     109     176      30     373     123     309    1105

Cuts at:   2241    2249    2697    2882    2923    2942    2964    3083    3186 
   Size:          8     448     185      41      19      22     119     103

  Fragments arranged by size: 

                1105     448     373     309     185     176     123     119
                 109     103      41      30      22      19      16       8
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1280    2792    3021    3053    3186 
   Size:       1280    1512     229      32     133

  Fragments arranged by size: 

                1512    1280     229     133      32
 
BsrBI CCG'CTC

Cuts at:      0    1770    2600    3186 
   Size:       1770     830     586

  Fragments arranged by size: 

                1770     830     586
 
BsrDI GCAATG_nn'

Cuts at:      0     503    3186 
   Size:        503    2683
 
BsrFI r'CCGG_y

Cuts at:      0    1377    3186 
   Size:       1377    1809
 
BssHII G'CGCG_C

Cuts at:      0    1683    3186 
   Size:       1683    1503
 
BssSI C'ACGA_G

Cuts at:      0    2062    2708    3186 
   Size:       2062     646     478

  Fragments arranged by size: 

                2062     646     478
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     392    1923    2232    2268    3186 
   Size:        392    1531     309      36     918

  Fragments arranged by size: 

                1531     918     392     309      36
 
BstEII G'GTnAC_C

Cuts at:      0    2007    3186 
   Size:       2007    1179
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2167    2454    3186 
   Size:       2167     287     732

  Fragments arranged by size: 

                2167     732     287
 
BstYI r'GATC_y

Cuts at:      0    1995    3186 
   Size:       1995    1191
 
Bsu36I CC'TnA_GG

Cuts at:      0    1108    1939    2927    3186 
   Size:       1108     831     988     259

  Fragments arranged by size: 

                1108     988     831     259
 
BtgI C'CryG_G

Cuts at:      0    1718    1787    2184    2432    2447    2609    2841    3186 
   Size:       1718      69     397     248      15     162     232     345

  Fragments arranged by size: 

                1718     397     345     248     232     162      69      15
            
 
BtsI GCAGTG_nn'

Cuts at:      0     275     870    1174    2024    2540    3170    3186 
   Size:        275     595     304     850     516     630      16

  Fragments arranged by size: 

                 850     630     595     516     304     275      16
 
DraIII CAC_nnn'GTG

Cuts at:      0    1740    3186 
   Size:       1740    1446
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      38    3186 
   Size:         38    3148
 
EaeI y'GGCC_r

Cuts at:      0      74     685     765     905     957    1610    1784    2169 
   Size:         74     611      80     140      52     653     174     385

Cuts at:   2169    2444    2595    3002    3186 
   Size:        275     151     407     184

  Fragments arranged by size: 

                 653     611     407     385     275     184     174     151
                 140      80      74      52
 
EagI C'GGCC_G

Cuts at:      0    1784    2595    3186 
   Size:       1784     811     591

  Fragments arranged by size: 

                1784     811     591
 
EarI CTCTTCn'nnn_

Cuts at:      0     319    1018    1465    1546    1993    2276    2303    3025 
   Size:        319     699     447      81     447     283      27     722

Cuts at:   3025    3186 
   Size:        161

  Fragments arranged by size: 

                 722     699     447     447     319     283     161      81
                  27
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1321    1579    2731    3186 
   Size:       1321     258    1152     455

  Fragments arranged by size: 

                1321    1152     455     258
 
EcoICRI GAG'CTC

Cuts at:      0     299    1134    2880    3186 
   Size:        299     835    1746     306

  Fragments arranged by size: 

                1746     835     306     299
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     733     822     900    1006    1135    1405    1612 
   Size:        641      92      89      78     106     129     270     207

Cuts at:   1612    1873    1948    2339    2800    3186 
   Size:        261      75     391     461     386

  Fragments arranged by size: 

                 641     461     391     386     270     261     207     129
                 106      92      89      78      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     136     799    1107    1221    2281    3063    3186 
   Size:        136     663     308     114    1060     782     123

  Fragments arranged by size: 

                1060     782     663     308     136     123     114
 
EcoO109I rG'GnC_Cy

Cuts at:      0     121     122     701    2094    2102    2147    2694    2960 
   Size:        121       1     579    1393       8      45     547     266

Cuts at:   2960    2961    3056    3186 
   Size:          1      95     130

  Fragments arranged by size: 

                1393     579     547     266     130     121      95      45
                   8       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     411    3186 
   Size:        411    2775
 
HaeII r_GCGC'y

Cuts at:      0     953    1314    1378    1411    1728    1885    1898    3186 
   Size:        953     361      64      33     317     157      13    1288

  Fragments arranged by size: 

                1288     953     361     317     157      64      33      13
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     646     678    1042    1074    2086    2118    2461    2493 
   Size:        646      32     364      32    1012      32     343      32

Cuts at:   2493    2512    2544    2584    2616    3034    3066    3186 
   Size:         19      32      40      32     418      32     120

  Fragments arranged by size: 

                1012     646     418     364     343     120      40      32
                  32      32      32      32      32      32      19
 
KasI G'GCGC_C

Cuts at:      0    1374    1894    3186 
   Size:       1374     520    1292

  Fragments arranged by size: 

                1374    1292     520
 
MluI A'CGCG_T

Cuts at:      0    1745    3186 
   Size:       1745    1441
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     767    3186 
   Size:        566     201    2419

  Fragments arranged by size: 

                2419     566     201
 
MscI TGG'CCA

Cuts at:      0    1612    2171    2446    3186 
   Size:       1612     559     275     740

  Fragments arranged by size: 

                1612     740     559     275
 
MslI CAynn'nnrTG

Cuts at:      0     176     374    2452    3186 
   Size:        176     198    2078     734

  Fragments arranged by size: 

                2078     734     198     176
 
MspA1I CmG'CkG

Cuts at:      0      19     108     691     842     857     890     920     984 
   Size:         19      89     583     151      15      33      30      64

Cuts at:    984    1124    1344    1626    1644    1761    1776    1920    2223 
   Size:        140     220     282      18     117      15     144     303

Cuts at:   2223    2232    2359    2393    2498    2763    2820    2945    2978 
   Size:          9     127      34     105     265      57     125      33

Cuts at:   2978    3186 
   Size:        208

  Fragments arranged by size: 

                 583     303     282     265     220     208     151     144
                 140     127     125     117     105      89      64      57
                  34      33      33      30      19      18      15      15
                   9
 
NarI GG'CG_CC

Cuts at:      0    1375    1895    3186 
   Size:       1375     520    1291

  Fragments arranged by size: 

                1375    1291     520
 
NcoI C'CATG_G

Cuts at:      0    2447    2609    2841    3186 
   Size:       2447     162     232     345

  Fragments arranged by size: 

                2447     345     232     162
 
NheI G'CTAG_C

Cuts at:      0    1363    3186 
   Size:       1363    1823
 
NsiI A_TGCA'T

Cuts at:      0     173    3186 
   Size:        173    3013
 
NspI r_CATG'y

Cuts at:      0     187     786    1515    1608    1755    3186 
   Size:        187     599     729      93     147    1431

  Fragments arranged by size: 

                1431     729     599     187     147      93
 
PasI CC'CwG_GG

Cuts at:      0    2098    2580    3065    3186 
   Size:       2098     482     485     121

  Fragments arranged by size: 

                2098     485     482     121
 
PciI A'CATG_T

Cuts at:      0     782    3186 
   Size:        782    2404
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2823    3186 
   Size:       2823     363
 
PfoI T'CCnGG_A

Cuts at:      0    1328    3186 
   Size:       1328    1858
 
PmlI CAC'GTG

Cuts at:      0    1559    1740    3186 
   Size:       1559     181    1446

  Fragments arranged by size: 

                1559    1446     181
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1300    1332    3186 
   Size:       1300      32    1854

  Fragments arranged by size: 

                1854    1300      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2094    2102    3186 
   Size:       2094       8    1084

  Fragments arranged by size: 

                2094    1084       8
 
PshAI GACnn'nnGTC

Cuts at:      0    2041    2498    3186 
   Size:       2041     457     688

  Fragments arranged by size: 

                2041     688     457
 
PspOMI G'GGCC_C

Cuts at:      0     121     700    2693    2938    2960    3186 
   Size:        121     579    1993     245      22     226

  Fragments arranged by size: 

                1993     579     245     226     121      22
 
PstI C_TGCA'G

Cuts at:      0    2273    3018    3186 
   Size:       2273     745     168

  Fragments arranged by size: 

                2273     745     168
 
PvuII CAG'CTG

Cuts at:      0     108     691     842     890     984    1124    1344    1920 
   Size:        108     583     151      48      94     140     220     576

Cuts at:   1920    2223    2232    2359    2763    2820    2945    3186 
   Size:        303       9     127     404      57     125     241

  Fragments arranged by size: 

                 583     576     404     303     241     220     151     140
                 127     125     108      94      57      48       9
 
SacI G_AGCT'C

Cuts at:      0     301    1136    2882    3186 
   Size:        301     835    1746     304

  Fragments arranged by size: 

                1746     835     304     301
 
SanDI GG'GwC_CC

Cuts at:      0    2094    3186 
   Size:       2094    1092
 
SapI GCTCTTCn'nnn_

Cuts at:      0     319    1465    3186 
   Size:        319    1146    1721

  Fragments arranged by size: 

                1721    1146     319
 
SbfI CC_TGCA'GG

Cuts at:      0    3018    3186 
   Size:       3018     168
 
SfcI C'TryA_G

Cuts at:      0     573    1005    1414    2141    2269    3014    3186 
   Size:        573     432     409     727     128     745     172

  Fragments arranged by size: 

                 745     727     573     432     409     172     128
 
SfoI GGC'GCC

Cuts at:      0    1376    1896    3186 
   Size:       1376     520    1290

  Fragments arranged by size: 

                1376    1290     520
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1377    3186 
   Size:       1377    1809
 
SmaI CCC'GGG

Cuts at:      0     816    1036    1422    3186 
   Size:        816     220     386    1764

  Fragments arranged by size: 

                1764     816     386     220
 
SmlI C'TyrA_G

Cuts at:      0    1651    2788    3186 
   Size:       1651    1137     398

  Fragments arranged by size: 

                1651    1137     398
 
SphI G_CATG'C

Cuts at:      0    1755    3186 
   Size:       1755    1431
 
StuI AGG'CCT

Cuts at:      0     142     335     571    1213    2176    3186 
   Size:        142     193     236     642     963    1010

  Fragments arranged by size: 

                1010     963     642     236     193     142
 
StyI C'CwwG_G

Cuts at:      0      77    1688    2447    2609    2841    3186 
   Size:         77    1611     759     162     232     345

  Fragments arranged by size: 

                1611     759     345     232     162      77
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1589    1687    2181    2854    3186 
   Size:       1589      98     494     673     332

  Fragments arranged by size: 

                1589     673     494     332      98
 
TatI w'GTAC_w

Cuts at:      0    1508    1976    3186 
   Size:       1508     468    1210

  Fragments arranged by size: 

                1508    1210     468
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1174    1206    3021    3053    3186 
   Size:       1174      32    1815      32     133

  Fragments arranged by size: 

                1815    1174     133      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     267    2439    3186 
   Size:        267    2172     747

  Fragments arranged by size: 

                2172     747     267
 
XmaI C'CCGG_G

Cuts at:      0     814    1034    1420    3186 
   Size:        814     220     386    1766

  Fragments arranged by size: 

                1766     814     386     220
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I     KasI     MluI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NheI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI     PstI
    PvuII     SacI    SanDI     SapI     SbfI     SfcI     SfoI    SgrAI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AleI     AlfI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI    BglII
    BmgBI    BsaBI    BsiWI    BspEI    BspHI    BsrGI    BstBI  BstZ17I
    BtgZI     ClaI    CspCI     DraI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     NaeI     NdeI
   NgoMIV     NotI     NruI     PacI     PmeI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     ScaI    SexAI     SfiI     SgfI
    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com