Restriction map for
pFN21AA0706
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BciVI
BclI BfrI BlnI BsaBI BsiEI
BsmI Bsp1407I BspDI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI KspI
MamI MfeI MluI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SapI SbfI
ScaI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1864 GTCGAC GT^[AC][GT]AC
AccII 385 CGCG CG^CG
AccIII 1637 TCCGGA T^CCGGA
AcsI 933 AAATTT [GA]^AATT[CT]
AflIII 269 ACGTGT A^C[GA][CT]GT
ApoI 933 AAATTT [GA]^AATT[CT]
AviII 2885 TGCGCA TGC^GCA
BalI 2971 TGGCCA TGG^CCA
BbeI 1195 GGCGCC GGCGC^C
BcgI 213 GCAAGTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 832 CTAG C^TAG
BsaAI 529 TACGTG [CT]AC^GT[GA]
BseAI 1637 TCCGGA T^CCGGA
BsiWI 527 CGTACG C^GTACG
BspEI 1637 TCCGGA T^CCGGA
BspHI 2631 TCATGA T^CATGA
BsrFI 3053 GCCGGC [GA]^CCGG[CT]
BstUI 385 CGCG CG^CG
Cfr10I 3053 GCCGGC [GA]^CCGG[CT]
DraIII 1498 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 2885 TGCGCA TGC^GCA
HincII 1864 GTCGAC GT[CT]^[GA]AC
HindII 1864 GTCGAC GT[CT]^[GA]AC
KasI 1195 GGCGCC G^GCGCC
MaeI 832 CTAG C^TAG
MluNI 2971 TGGCCA TGG^CCA
MroI 1637 TCCGGA T^CCGGA
MscI 2971 TGGCCA TGG^CCA
MseI 44 TTAA T^TAA
MvnI 385 CGCG CG^CG
NaeI 3053 GCCGGC GCC^GGC
NarI 1195 GGCGCC GG^CGCC
NdeI 1703 CATATG CA^TATG
NgoMIV 3053 GCCGGC G^CCGGC
PshAI 2115 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2631 TCATGA T^CATGA
SalI 1864 GTCGAC G^TCGAC
SexAI 1499 ACCTGGT A^CC[AT]GGT
SfiI 3215 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1195 GGCGCC GGC^GCC
StuI 1164 AGGCCT AGG^CCT
Tru9I 44 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Aor51HI 680 AGCGCT AGC^GCT
2045 AGCGCT
BanI 7 GGTGCC G^G[CT][GA]CC
1195 GGCGCC
BglII 443 AGATCT A^GATCT
2261 AGATCT
BlpI 406 GCTCAGC GC^T[ACGT]AGC
1354 GCTGAGC
Bpu1102I 406 GCTCAGC GC^T[ACGT]AGC
1354 GCTGAGC
BsgI 3134 CTGCAC GTGCAG(16/14)
532 GTGCAG
BsmBI 2378 GAGACG CGTCTC(1/5)
3261 GAGACG
CelII 406 GCTCAGC GC^T[ACGT]AGC
1354 GCTGAGC
Eco47III 680 AGCGCT AGC^GCT
2045 AGCGCT
EcoNI 1684 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2619 CCTGGCCCAGG
EcoT14I 1319 CCATGG C^C[AT][AT]GG
2228 CCATGG
HindIII 144 AAGCTT A^AGCTT
1717 AAGCTT
NcoI 1319 CCATGG C^CATGG
2228 CCATGG
StyI 1319 CCATGG C^C[AT][AT]GG
2228 CCATGG
TfiI 1953 GAATC G^A[AT]TC
2526 GATTC
XcmI 1548 CCACATCAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2749 CCACTGTCAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI