Restriction map for
pFN21AA0674
(Linear) (Six-base) MAPSORT of: KIAA0674.seq Check: 6244 from: 1 to: 3657
KIAA0674.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 486 2468 2946 3657
Size: 486 1982 478 711
Fragments arranged by size:
1982 711 486 478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 1550 1843 2050 2506 2761 3281 3657
Size: 1219 331 293 207 456 255 520 376
Fragments arranged by size:
1219 520 456 376 331 293 255 207
AgeI A'CCGG_T
Cuts at: 0 3394 3657
Size: 3394 263
AleI CACnn'nnGTG
Cuts at: 0 2065 3657
Size: 2065 1592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1660 3657
Size: 1660 1997
AlwNI CAG_nnn'CTG
Cuts at: 0 94 2511 3040 3657
Size: 94 2417 529 617
Fragments arranged by size:
2417 617 529 94
ApaI G_GGCC'C
Cuts at: 0 3119 3657
Size: 3119 538
ApoI r'AATT_y
Cuts at: 0 109 283 1553 2119 2288 3657
Size: 109 174 1270 566 169 1369
Fragments arranged by size:
1369 1270 566 174 169 109
AvaI C'yCGr_G
Cuts at: 0 2345 3304 3657
Size: 2345 959 353
Fragments arranged by size:
2345 959 353
AvrII C'CTAG_G
Cuts at: 0 3144 3657
Size: 3144 513
BanI G'GyrC_C
Cuts at: 0 52 1275 1305 2943 3657
Size: 52 1223 30 1638 714
Fragments arranged by size:
1638 1223 714 52 30
BanII G_rGCy'C
Cuts at: 0 1301 2090 3119 3143 3657
Size: 1301 789 1029 24 514
Fragments arranged by size:
1301 1029 789 514 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 2865 3578 3657
Size: 2865 713 79
Fragments arranged by size:
2865 713 79
BbvCI CC'TCA_GC
Cuts at: 0 1280 2883 3041 3353 3657
Size: 1280 1603 158 312 304
Fragments arranged by size:
1603 1280 312 304 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 643 677 812 846 1432 1466 3657
Size: 643 34 135 34 586 34 2191
Fragments arranged by size:
2191 643 586 135 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3165 3657
Size: 3165 492
BfrBI ATG'CAT
Cuts at: 0 244 3657
Size: 244 3413
BglI GCCn_nnn'nGGC
Cuts at: 0 61 1452 2545 3657
Size: 61 1391 1093 1112
Fragments arranged by size:
1391 1112 1093 61
BglII A'GATC_T
Cuts at: 0 2040 3657
Size: 2040 1617
BlpI GC'TnA_GC
Cuts at: 0 2396 3206 3657
Size: 2396 810 451
Fragments arranged by size:
2396 810 451
Bme1580I G_kGCm'C
Cuts at: 0 2948 3119 3657
Size: 2948 171 538
Fragments arranged by size:
2948 538 171
BmrI ACTGGGnnnn_n'
Cuts at: 0 1304 2403 3657
Size: 1304 1099 1254
Fragments arranged by size:
1304 1254 1099
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3066 3469 3657
Size: 3066 403 188
Fragments arranged by size:
3066 403 188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1285 2194 2527 2731 3657
Size: 761 524 909 333 204 926
Fragments arranged by size:
926 909 761 524 333 204
Bpu10I CC'TnA_GC
Cuts at: 0 1280 2883 3041 3137 3353 3657
Size: 1280 1603 158 96 216 304
Fragments arranged by size:
1603 1280 304 216 158 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 2143 2237 2888 3352 3657
Size: 803 1340 94 651 464 305
Fragments arranged by size:
1340 803 651 464 305 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 1959 3657
Size: 1959 1698
BsaHI Gr'CG_yC
Cuts at: 0 3594 3657
Size: 3594 63
BsaWI w'CCGG_w
Cuts at: 0 2141 3394 3657
Size: 2141 1253 263
Fragments arranged by size:
2141 1253 263
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1942 2191 2287 2290 2975 3190 3322 3574
Size: 1942 249 96 3 685 215 132 252
Cuts at: 3574 3577 3657
Size: 3 80
Fragments arranged by size:
1942 685 252 249 215 132 96 80
3 3
BseYI C'CCAG_C
Cuts at: 0 639 719 735 1436 1464 3460 3473 3651
Size: 639 80 16 701 28 1996 13 178
Cuts at: 3651 3657
Size: 6
Fragments arranged by size:
1996 701 639 178 80 28 16 13
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2089 3657
Size: 2089 1568
BsiHKAI G_wGCw'C
Cuts at: 0 211 545 2090 2459 3657
Size: 211 334 1545 369 1198
Fragments arranged by size:
1545 1198 369 334 211
BsmI GAATG_Cn'
Cuts at: 0 737 3657
Size: 737 2920
BsmBI CGTCTCn'nnnn_
Cuts at: 0 935 3657
Size: 935 2722
Bsp1286I G_dGCh'C
Cuts at: 0 211 545 1301 2090 2459 2948 3119 3143
Size: 211 334 756 789 369 489 171 24
Cuts at: 3143 3657
Size: 514
Fragments arranged by size:
789 756 514 489 369 334 211 171
24
BspHI T'CATG_A
Cuts at: 0 1523 1598 2669 2750 3657
Size: 1523 75 1071 81 907
Fragments arranged by size:
1523 1071 907 81 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 486 1381 1941 2468 2946 3418 3657
Size: 486 895 560 527 478 472 239
Fragments arranged by size:
895 560 527 486 478 472 239
BsrBI CCG'CTC
Cuts at: 0 47 1473 2850 3657
Size: 47 1426 1377 807
Fragments arranged by size:
1426 1377 807 47
BsrDI GCAATG_nn'
Cuts at: 0 503 3657
Size: 503 3154
BsrFI r'CCGG_y
Cuts at: 0 613 1239 3394 3657
Size: 613 626 2155 263
Fragments arranged by size:
2155 626 613 263
BstAPI GCAn_nnn'nTGC
Cuts at: 0 241 1312 3657
Size: 241 1071 2345
Fragments arranged by size:
2345 1071 241
BstBI TT'CG_AA
Cuts at: 0 1557 3657
Size: 1557 2100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 222 2620 3198 3657
Size: 222 2398 578 459
Fragments arranged by size:
2398 578 459 222
BstYI r'GATC_y
Cuts at: 0 1739 2040 3657
Size: 1739 301 1617
Fragments arranged by size:
1739 1617 301
Bsu36I CC'TnA_GG
Cuts at: 0 2987 3327 3557 3657
Size: 2987 340 230 100
Fragments arranged by size:
2987 340 230 100
BtgI C'CryG_G
Cuts at: 0 99 2939 3158 3657
Size: 99 2840 219 499
Fragments arranged by size:
2840 499 219 99
BtsI GCAGTG_nn'
Cuts at: 0 545 659 1943 2826 3657
Size: 545 114 1284 883 831
Fragments arranged by size:
1284 883 831 545 114
ClaI AT'CG_AT
Cuts at: 0 249 3657
Size: 249 3408
DraI TTT'AAA
Cuts at: 0 3516 3657
Size: 3516 141
DrdI GACnn_nn'nnGTC
Cuts at: 0 2349 3657
Size: 2349 1308
EaeI y'GGCC_r
Cuts at: 0 96 1940 2851 3657
Size: 96 1844 911 806
Fragments arranged by size:
1844 911 806 96
EarI CTCTTCn'nnn_
Cuts at: 0 531 1022 1238 1607 1837 2780 2804 2840
Size: 531 491 216 369 230 943 24 36
Cuts at: 2840 3494 3657
Size: 654 163
Fragments arranged by size:
943 654 531 491 369 230 216 163
36 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2433 3657
Size: 2433 1224
EcoICRI GAG'CTC
Cuts at: 0 2088 3657
Size: 2088 1569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1219 1285 1550 1843 2050 2194 2506
Size: 761 458 66 265 293 207 144 312
Cuts at: 2506 2527 2731 2761 3281 3657
Size: 21 204 30 520 376
Fragments arranged by size:
761 520 458 376 312 293 265 207
204 144 66 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 143 2176 3657
Size: 143 2033 1481
Fragments arranged by size:
2033 1481 143
EcoO109I rG'GnC_Cy
Cuts at: 0 552 2991 3115 3116 3148 3315 3331 3657
Size: 552 2439 124 1 32 167 16 326
Fragments arranged by size:
2439 552 326 167 124 32 16 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1759 2229 3657
Size: 1759 470 1428
Fragments arranged by size:
1759 1428 470
FspI TGC'GCA
Cuts at: 0 1949 2484 3657
Size: 1949 535 1173
Fragments arranged by size:
1949 1173 535
FspAI rTGC'GCAy
Cuts at: 0 2484 3657
Size: 2484 1173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 871 903 940 972 1333 1365
Size: 22 32 817 32 37 32 361 32
Cuts at: 1365 2194 2226 2365 2397 2757 2789 3205 3237
Size: 829 32 139 32 360 32 416 32
Cuts at: 3237 3657
Size: 420
Fragments arranged by size:
829 817 420 416 361 360 139 37
32 32 32 32 32 32 32 32
22
HincII GTy'rAC
Cuts at: 0 349 3657
Size: 349 3308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 567 3657
Size: 446 121 3090
Fragments arranged by size:
3090 446 121
MscI TGG'CCA
Cuts at: 0 98 3657
Size: 98 3559
MslI CAynn'nnrTG
Cuts at: 0 104 1687 2065 3657
Size: 104 1583 378 1592
Fragments arranged by size:
1592 1583 378 104
MspA1I CmG'CkG
Cuts at: 0 91 1146 1354 1440 1939 1968 2016 2368
Size: 91 1055 208 86 499 29 48 352
Cuts at: 2368 2379 2781 3657
Size: 11 402 876
Fragments arranged by size:
1055 876 499 402 352 208 91 86
48 29 11
NaeI GCC'GGC
Cuts at: 0 1241 3657
Size: 1241 2416
NcoI C'CATG_G
Cuts at: 0 99 2939 3158 3657
Size: 99 2840 219 499
Fragments arranged by size:
2840 499 219 99
NgoMIV G'CCGG_C
Cuts at: 0 1239 3657
Size: 1239 2418
NsiI A_TGCA'T
Cuts at: 0 246 3657
Size: 246 3411
NspI r_CATG'y
Cuts at: 0 1662 1900 3657
Size: 1662 238 1757
Fragments arranged by size:
1757 1662 238
PpuMI rG'GwC_Cy
Cuts at: 0 552 3315 3657
Size: 552 2763 342
Fragments arranged by size:
2763 552 342
PspOMI G'GGCC_C
Cuts at: 0 3115 3657
Size: 3115 542
PspXI vC'TCGA_Gb
Cuts at: 0 2345 3657
Size: 2345 1312
PstI C_TGCA'G
Cuts at: 0 297 924 1445 1967 2009 2373 2465 3657
Size: 297 627 521 522 42 364 92 1192
Fragments arranged by size:
1192 627 522 521 364 297 92 42
PvuII CAG'CTG
Cuts at: 0 91 1146 1354 1440 1968 2016 2368 2379
Size: 91 1055 208 86 528 48 352 11
Cuts at: 2379 2781 3657
Size: 402 876
Fragments arranged by size:
1055 876 528 402 352 208 91 86
48 11
SacI G_AGCT'C
Cuts at: 0 2090 3657
Size: 2090 1567
SanDI GG'GwC_CC
Cuts at: 0 3315 3657
Size: 3315 342
SapI GCTCTTCn'nnn_
Cuts at: 0 1022 2840 3657
Size: 1022 1818 817
Fragments arranged by size:
1818 1022 817
SexAI A'CCwGG_T
Cuts at: 0 2675 3657
Size: 2675 982
SfcI C'TryA_G
Cuts at: 0 293 414 578 920 1441 1963 2005 2369
Size: 293 121 164 342 521 522 42 364
Cuts at: 2369 2401 2461 3657
Size: 32 60 1196
Fragments arranged by size:
1196 522 521 364 342 293 164 121
60 42 32
SmlI C'TyrA_G
Cuts at: 0 818 2158 2252 2345 2903 3367 3657
Size: 818 1340 94 93 558 464 290
Fragments arranged by size:
1340 818 558 464 290 94 93
SphI G_CATG'C
Cuts at: 0 1900 3657
Size: 1900 1757
StyI C'CwwG_G
Cuts at: 0 99 1494 1916 2213 2447 2939 3144 3158
Size: 99 1395 422 297 234 492 205 14
Cuts at: 3158 3657
Size: 499
Fragments arranged by size:
1395 499 492 422 297 234 205 99
14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 996 3657
Size: 996 2661
TatI w'GTAC_w
Cuts at: 0 119 2388 3657
Size: 119 2269 1269
Fragments arranged by size:
2269 1269 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3154 3186 3657
Size: 3154 32 471
Fragments arranged by size:
3154 471 32
Tth111I GACn'n_nGTC
Cuts at: 0 1215 3657
Size: 1215 2442
XhoI C'TCGA_G
Cuts at: 0 2345 3657
Size: 2345 1312
XmnI GAAnn'nnTTC
Cuts at: 0 2862 3657
Size: 2862 795
Enzymes that do cut:
AarI AcuI AgeI AleI AlfI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI
ClaI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI FspAI Hin4I HincII MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PpuMI PspOMI PspXI PstI PvuII SacI SanDI SapI
SexAI SfcI SmlI SphI StyI TaqII TatI TstI
Tth111I XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BclI BmgBI BmtI BsaAI BsaBI BsaXI BsiEI BsiWI
BspEI BsrGI BssHII BssSI BstEII BstZ17I BtgZI CspCI
DraIII EagI EcoRI EcoRV FseI HaeII HindIII HpaI
KasI KpnI MfeI MluI NarI NdeI NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI XbaI XcmI XmaI
ZraI