Restriction map for
pFN21AA0651
(Circular) (Six-base) MAPSORT of: pFN21AA0651.seq Check: 2737 from: 1 to: 7945
pFN21AA0651 7945 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5755 278
Size: 53 83 186 936 4219 2468
Fragments arranged by size:
4219 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5288 1937
Size: 3351 4594
Acc65I G'GTAC_C
Cuts at: 2285 5266 2285
Size: 2981 4964
AclI AA'CG_TT
Cuts at: 1563 6072 6445 1563
Size: 4509 373 3063
Fragments arranged by size:
4509 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2302 2606 3068 3825 6008 7392 7660 1632
Size: 670 304 462 757 2183 1384 268 1917
Fragments arranged by size:
2183 1917 1384 757 670 462 304 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7748
AflIII A'CryG_T
Cuts at: 1203 6844 7821 1203
Size: 5641 977 1327
Fragments arranged by size:
5641 1327 977
AgeI A'CCGG_T
Cuts at: 6776 6776
Size: 7945
AhdI GACnn_n'nnGTC
Cuts at: 6674 6674
Size: 7945
AleI CACnn'nnGTG
Cuts at: 1166 2079 1166
Size: 913 7032
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2978 3095 1184
Size: 1794 117 6034
Fragments arranged by size:
6034 1794 117
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4568 4600 4568
Size: 32 7913
AlwNI CAG_nnn'CTG
Cuts at: 3121 3199 3529 3659 5074 5688 7260 3121
Size: 78 330 130 1415 614 1572 3806
Fragments arranged by size:
3806 1572 1415 614 330 130 78
ApaI G_GGCC'C
Cuts at: 3607 3646 3671 4598 4915 3607
Size: 39 25 927 317 6637
Fragments arranged by size:
6637 927 317 39 25
ApaLI G'TGCA_C
Cuts at: 3938 6002 7158 3938
Size: 2064 1156 4725
Fragments arranged by size:
4725 2064 1156
ApoI r'AATT_y
Cuts at: 1493 5254 5480 5632 7741 1493
Size: 3761 226 152 2109 1697
Fragments arranged by size:
3761 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6499 160
Size: 872 5467 1606
Fragments arranged by size:
5467 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7945
AvaI C'yCGr_G
Cuts at: 1870 1943 2289 2329 2355 3357 3639 3951 4326
Size: 73 346 40 26 1002 282 312 375
Cuts at: 4326 4632 4821 5270 1870
Size: 306 189 449 4545
Fragments arranged by size:
4545 1002 449 375 346 312 306 282
189 73 40 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2277 2310 3416 3449 2277
Size: 33 1106 33 6773
Fragments arranged by size:
6773 1106 33 33
BamHI G'GATC_C
Cuts at: 2796 3972 4927 5275 5642 2796
Size: 1176 955 348 367 5099
Fragments arranged by size:
5099 1176 955 367 348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2285 2406 5266 6721 618
Size: 325 217 627 498 121 2860 1455 1842
Fragments arranged by size:
2860 1842 1455 627 498 325 217 121
BanII G_rGCy'C
Cuts at: 1391 2699 3144 3607 3646 3671 4598 4915 5084
Size: 1308 445 463 39 25 927 317 169
Cuts at: 5084 5264 1391
Size: 180 4072
Fragments arranged by size:
4072 1308 927 463 445 317 180 169
39 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3737 928
Size: 971 1838 5136
Fragments arranged by size:
5136 1838 971
BbvCI CC'TCA_GC
Cuts at: 3167 4899 3167
Size: 1732 6213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6136 6170 1903
Size: 34 50 34 4115 34 3678
Fragments arranged by size:
4115 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2203 2236 5837 7047 2203
Size: 33 3601 1210 3101
Fragments arranged by size:
3601 3101 1210 33
BclI T'GATC_A
Cuts at: 1548 3906 1548
Size: 2358 5587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3675 4670 4850
Size: 107 122 71 631 605 2003 995 180
Cuts at: 4850 4910 6556 136
Size: 60 1646 1525
Fragments arranged by size:
2003 1646 1525 995 631 605 180 122
107 71 60
BglII A'GATC_T
Cuts at: 3618 7940 3618
Size: 4322 3623
BlpI GC'TnA_GC
Cuts at: 5362 5362
Size: 7945
Bme1580I G_kGCm'C
Cuts at: 1790 2409 3206 3607 3646 3671 3942 4096 4598
Size: 619 797 401 39 25 271 154 502
Cuts at: 4598 4828 4915 6006 7162 1790
Size: 230 87 1091 1156 2573
Fragments arranged by size:
2573 1156 1091 797 619 502 401 271
230 154 87 39 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7945
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2224 3551 3647
Size: 416 53 83 399 24 807 1327 96
Cuts at: 3647 5084 5676 6634 442
Size: 1437 592 958 1753
Fragments arranged by size:
1753 1437 1327 958 807 592 416 399
96 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3614 3779 4811 4853 6605 1239
Size: 2375 165 1032 42 1752 2579
Fragments arranged by size:
2579 2375 1752 1032 165 42
Bpu10I CC'TnA_GC
Cuts at: 3167 4599 4899 3167
Size: 1432 300 6213
Fragments arranged by size:
6213 1432 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3395 4244 4990 6070 6935 7233 3395
Size: 849 746 1080 865 298 4107
Fragments arranged by size:
4107 1080 865 849 746 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6608 882
Size: 617 5109 2219
Fragments arranged by size:
5109 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5736 493
Size: 711 4532 2702
Fragments arranged by size:
4532 2702 711
BsaBI GATnn'nnATC
Cuts at: 7939 7939
Size: 7945
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3190 5752 6134 6769
Size: 53 83 186 936 1657 2562 382 635
Cuts at: 6769 275
Size: 1451
Fragments arranged by size:
2562 1657 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2339 3384 3960 6378 6776 7050 7197 7608 2339
Size: 1045 576 2418 398 274 147 411 2676
Fragments arranged by size:
2676 2418 1045 576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3272 3302 3548 3578 4601
Size: 30 165 30 1869 30 246 30 1023
Cuts at: 4601 4631 1178
Size: 30 4492
Fragments arranged by size:
4492 1869 1023 246 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2290 2704 3527 3530 4508 4748 5144 5216
Size: 1104 414 823 3 978 240 396 72
Cuts at: 5216 1186
Size: 3915
Fragments arranged by size:
3915 1104 978 823 414 396 240 72
3
BseYI C'CCAG_C
Cuts at: 4557 4655 4894 4991 7148 7644 4557
Size: 98 239 97 2157 496 4858
Fragments arranged by size:
4858 2157 496 239 98 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3014 1163
Size: 573 1278 6094
Fragments arranged by size:
6094 1278 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5395 6156 7184 1540
Size: 275 186 3394 761 1028 2301
Fragments arranged by size:
3394 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3144 3411 3942 3968 6006 6091 7162 3144
Size: 267 531 26 2038 85 1071 3927
Fragments arranged by size:
3927 2038 1071 531 267 85 26
BsiWI C'GTAC_G
Cuts at: 3868 3868
Size: 7945
BsmI GAATG_Cn'
Cuts at: 3496 5190 5456 5549 3496
Size: 1694 266 93 5892
Fragments arranged by size:
5892 1694 266 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5198 5198
Size: 7945
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 2699 3144 3206 3411 3607 3646
Size: 399 619 290 445 62 205 196 39
Cuts at: 3646 3671 3942 3968 4096 4598 4828 4915 5084
Size: 25 271 26 128 502 230 87 169
Cuts at: 5084 5264 6006 6091 7162 1391
Size: 180 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 619 502 445 399 290
271 230 205 196 180 169 128 87
85 62 39 26 25
BspEI T'CCGG_A
Cuts at: 3384 7608 3384
Size: 4224 3721
BspHI T'CATG_A
Cuts at: 3054 5834 7564 3054
Size: 2780 1730 3435
Fragments arranged by size:
3435 2780 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2507 4062 4296 5300 844
Size: 1663 1555 234 1004 3489
Fragments arranged by size:
3489 1663 1555 1004 234
BsrBI CCG'CTC
Cuts at: 1394 1823 2644 2712 4212 4243 4603 5092 5832
Size: 429 821 68 1500 31 360 489 740
Cuts at: 5832 1394
Size: 3507
Fragments arranged by size:
3507 1500 821 740 489 429 360 68
31
BsrDI GCAATG_nn'
Cuts at: 65 1248 2509 2751 3876 6440 6614 65
Size: 1183 1261 242 1125 2564 174 1396
Fragments arranged by size:
2564 1396 1261 1183 1125 242 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4749 6589 6776 1700
Size: 110 2939 1840 187 2869
Fragments arranged by size:
2939 2869 1840 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7945
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7945
BssSI C'ACGA_G
Cuts at: 4233 4369 5169 6005 7017 4233
Size: 136 800 836 1012 5161
Fragments arranged by size:
5161 1012 836 800 136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3994 4958 794
Size: 3200 964 3781
Fragments arranged by size:
3781 3200 964
BstBI TT'CG_AA
Cuts at: 2620 6749 2620
Size: 4129 3816
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7945
BstXI CCAn_nnnn'nTGG
Cuts at: 3541 4696 3541
Size: 1155 6790
BstYI r'GATC_y
Cuts at: 1972 2038 2796 3618 3972 4927 5014 5275 5642
Size: 66 758 822 354 955 87 261 367
Cuts at: 5642 6027 6044 7496 7582 7594 7940 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 955 822 758 385 367 354
346 261 87 86 66 17 12
Bsu36I CC'TnA_GG
Cuts at: 2249 4590 2249
Size: 2341 5604
BtgI C'CryG_G
Cuts at: 513 1065 2704 2847 3502 4689 513
Size: 552 1639 143 655 1187 3769
Fragments arranged by size:
3769 1639 1187 655 552 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3218 4971 487
Size: 683 56 1992 1753 3461
Fragments arranged by size:
3461 1992 1753 683 56
BtsI GCAGTG_nn'
Cuts at: 4177 4383 4826 5113 5461 6255 6275 4177
Size: 206 443 287 348 794 20 5847
Fragments arranged by size:
5847 794 443 348 287 206 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2734 2769 529
Size: 35 2170 35 5705
Fragments arranged by size:
5705 2170 35 35
DraI TTT'AAA
Cuts at: 5249 5249
Size: 7945
DraIII CAC_nnn'GTG
Cuts at: 3037 3037
Size: 7945
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6952 809
Size: 507 5636 1802
Fragments arranged by size:
5636 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2645 2655
Size: 54 1425 50 275 18 315 500 10
Cuts at: 2655 2707 3676 4866 5392 6281 7910 8
Size: 52 969 1190 526 889 1629 43
Fragments arranged by size:
1629 1425 1190 969 889 526 500 315
275 54 52 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 5392 1537
Size: 275 3580 4090
Fragments arranged by size:
4090 3580 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 2417 2622 2862 3071 3081 4152 4842
Size: 681 383 205 240 209 10 1071 690
Cuts at: 4842 5082 5875 1353
Size: 240 793 3423
Fragments arranged by size:
3423 1071 793 690 681 383 240 240
209 205 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5145 6532 6906 7052 387
Size: 1064 138 3556 1387 374 146 1280
Fragments arranged by size:
3556 1387 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3142 3142
Size: 7945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2302 2606 3068 3614 3779 3825 4811
Size: 393 670 304 462 546 165 46 986
Cuts at: 4811 4853 6008 6605 7392 7660 1239
Size: 42 1155 597 787 268 1524
Fragments arranged by size:
1524 1155 986 787 670 597 546 462
393 304 268 165 46 42
EcoO109I rG'GnC_Cy
Cuts at: 2013 2024 3118 3196 3269 3526 3603 3667 3668
Size: 11 1094 78 73 257 77 64 1
Cuts at: 3668 4201 4594 4595 4903 2013
Size: 533 393 1 308 5055
Fragments arranged by size:
5055 1094 533 393 308 257 78 77
73 64 11 1 1
EcoRI G'AATT_C
Cuts at: 5254 5632 5254
Size: 378 7567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3688 3835 3688
Size: 147 7798
FspI TGC'GCA
Cuts at: 3998 6451 3998
Size: 2453 5492
FspAI rTGC'GCAy
Cuts at: 3998 3998
Size: 7945
HaeII r_GCGC'y
Cuts at: 1678 3523 3748 4747 7092 1678
Size: 1845 225 999 2345 2531
Fragments arranged by size:
2531 2345 1845 999 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3548 3580 4104 4136 4430
Size: 32 135 32 1976 32 524 32 294
Cuts at: 4430 4462 6586 6618 6660 6692 1373
Size: 32 2124 32 42 32 2626
Fragments arranged by size:
2626 2124 1976 524 294 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5289 5535 669
Size: 988 281 3351 246 3079
Fragments arranged by size:
3351 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7945
HpaI GTT'AAC
Cuts at: 5535 5535
Size: 7945
KpnI G_GTAC'C
Cuts at: 2289 5270 2289
Size: 2981 4964
MfeI C'AATT_G
Cuts at: 5544 5544
Size: 7945
MluI A'CGCG_T
Cuts at: 7821 7821
Size: 7945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2694 7059 7243 1305
Size: 1389 4365 184 2007
Fragments arranged by size:
4365 2007 1389 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6123
Fragments arranged by size:
6123 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2153 2441 2766 3077 4571 5903
Size: 648 913 74 288 325 311 1494 1332
Cuts at: 5903 6262 6421 518
Size: 359 159 2042
Fragments arranged by size:
2042 1494 1332 913 648 359 325 311
288 159 74
MspA1I CmG'CkG
Cuts at: 1606 2269 2523 2927 3592 4359 4477 4550 4711
Size: 663 254 404 665 767 118 73 161
Cuts at: 4711 4864 4898 5361 6038 7186 7431 1606
Size: 153 34 463 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 665 663 463 404
254 245 161 153 118 73 34
NaeI GCC'GGC
Cuts at: 4751 4751
Size: 7945
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7393
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7945
NgoMIV G'CCGG_C
Cuts at: 4749 4749
Size: 7945
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7945
NotI GC'GGCC_GC
Cuts at: 5392 5392
Size: 7945
NspI r_CATG'y
Cuts at: 1132 1229 2158 2830 4005 4721 5303 6848 1132
Size: 97 929 672 1175 716 582 1545 2229
Fragments arranged by size:
2229 1545 1175 929 716 672 582 97
PasI CC'CwG_GG
Cuts at: 1348 2782 4197 4873 4908 1348
Size: 1434 1415 676 35 4385
Fragments arranged by size:
4385 1434 1415 676 35
PciI A'CATG_T
Cuts at: 6844 6844
Size: 7945
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2126 3238 3659 1108
Size: 1018 1112 421 5394
Fragments arranged by size:
5394 1112 1018 421
PfoI T'CCnGG_A
Cuts at: 2637 3795 4803 5196 2637
Size: 1158 1008 393 5386
Fragments arranged by size:
5386 1158 1008 393
PmeI GTTT'AAAC
Cuts at: 5249 5249
Size: 7945
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6014 6046 7527 7559 6014
Size: 32 1481 32 6400
Fragments arranged by size:
6400 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3269 3526 3269
Size: 257 7688
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7945
PsiI TTA'TAA
Cuts at: 5515 5515
Size: 7945
PspOMI G'GGCC_C
Cuts at: 3603 3642 3667 4594 4911 3603
Size: 39 25 927 317 6637
Fragments arranged by size:
6637 927 317 39 25
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4686 4718 4686
Size: 32 7913
PstI C_TGCA'G
Cuts at: 830 2038 2264 2967 3314 3495 3615 3858 4254
Size: 1208 226 703 347 181 120 243 396
Cuts at: 4254 4433 4710 4731 5297 830
Size: 179 277 21 566 3478
Fragments arranged by size:
3478 1208 703 566 396 347 277 243
226 181 179 120 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7945
PvuII CAG'CTG
Cuts at: 2269 2927 3592 4477 4550 4864 4898 2269
Size: 658 665 885 73 314 34 5316
Fragments arranged by size:
5316 885 665 658 314 73 34
SacI G_AGCT'C
Cuts at: 3144 3144
Size: 7945
SalI G'TCGA_C
Cuts at: 1936 5287 1936
Size: 3351 4594
SapI GCTCTTCn'nnn_
Cuts at: 3081 5082 3081
Size: 2001 5944
SbfI CC_TGCA'GG
Cuts at: 5297 5297
Size: 7945
ScaI AGT'ACT
Cuts at: 6193 7905 6193
Size: 1712 6233
SfcI C'TryA_G
Cuts at: 826 1045 2034 2260 2878 2963 3310 3491 3611
Size: 219 989 226 618 85 347 181 120
Cuts at: 3611 3854 4250 4429 4541 4706 4727 5229 5293
Size: 243 396 179 112 165 21 502 64
Cuts at: 5293 6428 7109 7300 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 989 681 618 502 396 347
243 226 219 191 181 179 165 120
112 85 64 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 3675 4910 3675
Size: 1235 6710
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7945
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7945
SmaI CCC'GGG
Cuts at: 1872 2291 2357 3953 4823 5272 1872
Size: 419 66 1596 870 449 4545
Fragments arranged by size:
4545 1596 870 449 419 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3410 4223 4969 6049 6950 7212
Size: 197 926 1467 813 746 1080 901 262
Cuts at: 7212 820
Size: 1553
Fragments arranged by size:
1553 1467 1080 926 901 813 746 262
197
SnaBI TAC'GTA
Cuts at: 493 5736 493
Size: 5243 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7945
SphI G_CATG'C
Cuts at: 1132 2158 5303 1132
Size: 1026 3145 3774
Fragments arranged by size:
3774 3145 1026
SrfI GCCC'GGGC
Cuts at: 4823 4823
Size: 7945
SspI AAT'ATT
Cuts at: 5 52 5869 5
Size: 47 5817 2081
Fragments arranged by size:
5817 2081 47
StuI AGG'CCT
Cuts at: 1517 3329 1517
Size: 1812 6133
StyI C'CwwG_G
Cuts at: 513 1065 2017 2382 2475 2550 4344 5384 513
Size: 552 952 365 93 75 1794 1040 3074
Fragments arranged by size:
3074 1794 1040 952 552 365 93 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3230 4898 5073 5972 5989 6142 6327
Size: 1255 1650 1668 175 899 17 153 185
Cuts at: 6327 6772 325
Size: 445 1498
Fragments arranged by size:
1668 1650 1498 1255 899 445 185 175
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3781 3813 4965
Size: 274 80 33 51 1443 1804 32 1152
Cuts at: 4965 6191 7903 96
Size: 1226 1712 138
Fragments arranged by size:
1804 1712 1443 1226 1152 274 138 80
51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3932 3964 4601 4633 7905 7937 3272
Size: 32 628 32 637 32 3272 32 3280
Fragments arranged by size:
3280 3272 637 628 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7945
XbaI T'CTAG_A
Cuts at: 5281 5281
Size: 7945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2147 4267 2147
Size: 2120 5825
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7945
XmaI C'CCGG_G
Cuts at: 1870 2289 2355 3951 4821 5270 1870
Size: 419 66 1596 870 449 4545
Fragments arranged by size:
4545 1596 870 449 419 66
XmnI GAAnn'nnTTC
Cuts at: 6074 6074
Size: 7945
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5753 276
Size: 53 83 186 936 4219 2468
Fragments arranged by size:
4219 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BfrBI BplI BstZ17I
EcoNI EcoRV FseI KasI NarI NruI NsiI PacI
PmlI PspXI RsrII SacII SanDI SexAI SfoI SwaI