Restriction map for
pFN21AA0583
(Circular) (Six-base) MAPSORT of: pFN21AA0583.seq Check: 7511 from: 1 to: 10462
pFN21AA0583 10462 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2256 2857 3226 5274 5644 2256
Size: 601 369 2048 370 7074
Fragments arranged by size:
7074 2048 601 370 369
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8272 278
Size: 53 83 186 936 6736 2468
Fragments arranged by size:
6736 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7371 7805 1937
Size: 5434 434 4594
Fragments arranged by size:
5434 4594 434
Acc65I G'GTAC_C
Cuts at: 6347 7783 6347
Size: 1436 9026
AclI AA'CG_TT
Cuts at: 1563 8589 8962 1563
Size: 7026 373 3063
Fragments arranged by size:
7026 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 2744 3071 3551 3761 4403 4839 5262
Size: 965 147 327 480 210 642 436 423
Cuts at: 5262 6728 6752 7029 8525 9909 10177 1632
Size: 1466 24 277 1496 1384 268 1917
Fragments arranged by size:
1917 1496 1466 1384 965 642 480 436
423 327 277 268 210 147 24
AfeI AGC'GCT
Cuts at: 2109 2109
Size: 10462
AflII C'TTAA_G
Cuts at: 820 1017 4009 820
Size: 197 2992 7273
Fragments arranged by size:
7273 2992 197
AflIII A'CryG_T
Cuts at: 1203 6505 9361 10338 1203
Size: 5302 2856 977 1327
Fragments arranged by size:
5302 2856 1327 977
AgeI A'CCGG_T
Cuts at: 9293 9293
Size: 10462
AhdI GACnn_n'nnGTC
Cuts at: 4318 5938 9191 4318
Size: 1620 3253 5589
Fragments arranged by size:
5589 3253 1620
AleI CACnn'nnGTG
Cuts at: 1166 3236 3977 4871 5117 5564 6552 1166
Size: 2070 741 894 246 447 988 5076
Fragments arranged by size:
5076 2070 988 894 741 447 246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6224 1184
Size: 5040 5422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 10430
AlwNI CAG_nnn'CTG
Cuts at: 2050 2215 2617 2767 3529 4817 4877 4988 5401
Size: 165 402 150 762 1288 60 111 413
Cuts at: 5401 5721 6187 7052 8205 9777 2050
Size: 320 466 865 1153 1572 2735
Fragments arranged by size:
2735 1572 1288 1153 865 762 466 413
402 320 165 150 111 60
ApaI G_GGCC'C
Cuts at: 4778 6805 4778
Size: 2027 8435
ApaLI G'TGCA_C
Cuts at: 6296 6545 8519 9675 6296
Size: 249 1974 1156 7083
Fragments arranged by size:
7083 1974 1156 249
ApoI r'AATT_y
Cuts at: 1493 6983 7771 7997 8149 10258 1493
Size: 5490 788 226 152 2109 1697
Fragments arranged by size:
5490 2109 1697 788 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9016 160
Size: 872 7984 1606
Fragments arranged by size:
7984 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10462
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 2093 2475 3949 5146 5174 5643
Size: 73 141 9 382 1474 1197 28 469
Cuts at: 5643 6008 6167 7787 1870
Size: 365 159 1620 4545
Fragments arranged by size:
4545 1620 1474 1197 469 382 365 159
141 73 28 9
BamHI G'GATC_C
Cuts at: 7792 8159 7792
Size: 367 10095
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 2392 5461 6347 6374
Size: 325 217 627 366 239 3069 886 27
Cuts at: 6374 7783 9238 618
Size: 1409 1455 1842
Fragments arranged by size:
3069 1842 1455 1409 886 627 366 325
239 217 27
BanII G_rGCy'C
Cuts at: 1391 2013 2493 3750 4403 4471 4685 4778 5184
Size: 622 480 1257 653 68 214 93 406
Cuts at: 5184 5451 5573 5870 6074 6805 7681 7781 1391
Size: 267 122 297 204 731 876 100 4072
Fragments arranged by size:
4072 1257 876 731 653 622 480 406
297 267 214 204 122 100 93 68
BbeI G_GCGC'C
Cuts at: 2157 2396 2157
Size: 239 10223
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2240 3248 4569 6497 6505 928
Size: 971 341 1008 1321 1928 8 4885
Fragments arranged by size:
4885 1928 1321 1008 971 341 8
BbvCI CC'TCA_GC
Cuts at: 2366 2744 3681 5965 2366
Size: 378 937 2284 6863
Fragments arranged by size:
6863 2284 937 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3237 3271 3489 3523 5137 5171 5315
Size: 34 1300 34 218 34 1614 34 144
Cuts at: 5315 5349 5451 5485 5785 5819 6076 6110 8653
Size: 34 102 34 300 34 257 34 2543
Cuts at: 8653 8687 1903
Size: 34 3678
Fragments arranged by size:
3678 2543 1614 1300 300 257 218 144
102 34 34 34 34 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8354 9564 8354
Size: 1210 9252
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4082 9073 136
Size: 107 122 71 631 605 2410 4991 1525
Fragments arranged by size:
4991 2410 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7701 10457 7701
Size: 2756 7706
BlpI GC'TnA_GC
Cuts at: 4859 6305 6998 7879 4859
Size: 1446 693 881 7442
Fragments arranged by size:
7442 1446 881 693
Bme1580I G_kGCm'C
Cuts at: 1790 2381 2857 4778 5464 5534 6300 6549 6805
Size: 591 476 1921 686 70 766 249 256
Cuts at: 6805 6893 8523 9679 1790
Size: 88 1630 1156 2573
Fragments arranged by size:
2573 1921 1630 1156 766 686 591 476
256 249 88 70
BmgBI CAC'GTC
Cuts at: 1318 2734 4732 5167 6667 7255 1318
Size: 1416 1998 435 1500 588 4525
Fragments arranged by size:
4525 1998 1500 1416 588 435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2029 3586 3763
Size: 416 53 83 399 24 612 1557 177
Cuts at: 3763 3917 4653 5349 5667 6274 8193 9151 442
Size: 154 736 696 318 607 1919 958 1753
Fragments arranged by size:
1919 1753 1557 958 736 696 612 607
416 399 318 177 154 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10462
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 4903 5113 6168 2467
Size: 2436 210 1055 6761
Fragments arranged by size:
6761 2436 1055 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 3038 3527 4226 5108 5361 5423 5435
Size: 1010 789 489 699 882 253 62 12
Cuts at: 5435 5758 6209 6347 6448 6518 6554 6668 6973
Size: 323 451 138 101 70 36 114 305
Cuts at: 6973 9122 1239
Size: 2149 2579
Fragments arranged by size:
2579 2149 1010 882 789 699 489 451
323 305 253 138 114 101 70 62
36 12
Bpu10I CC'TnA_GC
Cuts at: 2366 2744 3008 3681 4679 5696 5708 5965 2366
Size: 378 264 673 998 1017 12 257 6863
Fragments arranged by size:
6863 1017 998 673 378 264 257 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 3368 3734 7331 7652 8587 9452 9750 2159
Size: 1209 366 3597 321 935 865 298 2871
Fragments arranged by size:
3597 2871 1209 935 865 366 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4757 7554 9125 882
Size: 617 3258 2797 1571 2219
Fragments arranged by size:
3258 2797 2219 1571 617
BsaAI yAC'GTr
Cuts at: 493 1204 6055 6295 6868 8253 493
Size: 711 4851 240 573 1385 2702
Fragments arranged by size:
4851 2702 1385 711 573 240
BsaBI GATnn'nnATC
Cuts at: 10456 10456
Size: 10462
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2154 2274 2393 2958
Size: 53 83 186 936 621 120 119 565
Cuts at: 2958 5692 6723 7415 8269 8651 9286 275
Size: 2734 1031 692 854 382 635 1451
Fragments arranged by size:
2734 1451 1031 936 854 692 635 621
565 382 186 120 119 83 53
BsaWI w'CCGG_w
Cuts at: 3624 8895 9293 9567 9714 10125 3624
Size: 5271 398 274 147 411 3961
Fragments arranged by size:
5271 3961 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2394 2424 3773 3803 4508
Size: 30 165 30 991 30 1349 30 705
Cuts at: 4508 4538 5285 5315 6821 6851 7437 7467 1178
Size: 30 747 30 1506 30 586 30 4173
Fragments arranged by size:
4173 1506 1349 991 747 705 586 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2402 2669 2837 3047 3139 3287 3434
Size: 970 246 267 168 210 92 148 147
Cuts at: 3434 4079 4393 5248 5898 6056 6767 7689 1186
Size: 645 314 855 650 158 711 922 3959
Fragments arranged by size:
3959 970 922 855 711 650 645 314
267 246 210 168 158 148 147 92
BseYI C'CCAG_C
Cuts at: 2013 2856 3025 3072 3467 5007 5320 5555 6082
Size: 843 169 47 395 1540 313 235 527
Cuts at: 6082 6445 6528 6959 7329 9665 10161 2013
Size: 363 83 431 370 2336 496 2314
Fragments arranged by size:
2336 2314 1540 843 527 496 431 395
370 363 313 235 169 83 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4148 6083 6758 7038 7195 1163
Size: 573 2412 1935 675 280 157 4430
Fragments arranged by size:
4430 2412 1935 675 573 280 157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5366 6814 7912 8673 9701 1540
Size: 275 186 3365 1448 1098 761 1028 2301
Fragments arranged by size:
3365 2301 1448 1098 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2493 3750 4403 4685 5451 5573 6300 6549
Size: 408 1257 653 282 766 122 727 249
Cuts at: 6549 8523 8608 9679 2085
Size: 1974 85 1071 2868
Fragments arranged by size:
2868 1974 1257 1071 766 727 653 408
282 249 122 85
BsmI GAATG_Cn'
Cuts at: 5479 5903 6934 7597 7973 8066 5479
Size: 424 1031 663 376 93 7875
Fragments arranged by size:
7875 1031 663 424 376 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3875 6130 3875
Size: 2255 8207
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2085 2381 2493 2857 3750 4403
Size: 399 223 72 296 112 364 893 653
Cuts at: 4403 4471 4685 4778 5184 5451 5464 5534 5573
Size: 68 214 93 406 267 13 70 39
Cuts at: 5573 5870 6074 6300 6549 6805 6893 7681 7781
Size: 297 204 226 249 256 88 788 100
Cuts at: 7781 8523 8608 9679 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 893 788 742 653 406 399
364 297 296 267 256 249 226 223
214 204 112 100 93 88 85 72
70 68 39 13
BspEI T'CCGG_A
Cuts at: 3624 10125 3624
Size: 6501 3961
BspHI T'CATG_A
Cuts at: 3309 8351 10081 3309
Size: 5042 1730 3690
Fragments arranged by size:
5042 3690 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 2256 2616 2790 2857 3189 3226 4627
Size: 1379 33 360 174 67 332 37 1401
Cuts at: 4627 4983 5274 5644 5729 6216 6573 7128 7638
Size: 356 291 370 85 487 357 555 510
Cuts at: 7638 7817 844
Size: 179 3489
Fragments arranged by size:
3489 1401 1379 555 510 487 370 360
357 356 332 291 179 174 85 67
37 33
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 2197 3277 3655 4692 6016 7217
Size: 429 302 72 1080 378 1037 1324 1201
Cuts at: 7217 7345 7483 7759 8349 1394
Size: 128 138 276 590 3507
Fragments arranged by size:
3507 1324 1201 1080 1037 590 429 378
302 276 138 128 72
BsrDI GCAATG_nn'
Cuts at: 65 1248 3993 4302 5220 5975 7461 7716 8957
Size: 1183 2745 309 918 755 1486 255 1241
Cuts at: 8957 9131 65
Size: 174 1396
Fragments arranged by size:
2745 1486 1396 1241 1183 918 755 309
255 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2421 2697 3567 4740 5269 5337 5487
Size: 110 611 276 870 1173 529 68 150
Cuts at: 5487 6344 6813 7059 9106 9293 1700
Size: 857 469 246 2047 187 2869
Fragments arranged by size:
2869 2047 1173 870 857 611 529 469
276 246 187 150 110 68
BsrGI T'GTAC_A
Cuts at: 96 3288 96
Size: 3192 7270
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10462
BssSI C'ACGA_G
Cuts at: 2819 4405 6626 8522 9534 2819
Size: 1586 2221 1896 1012 3747
Fragments arranged by size:
3747 2221 1896 1586 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2617 2767 7296 794
Size: 1823 150 4529 3960
Fragments arranged by size:
4529 3960 1823 150
BstBI TT'CG_AA
Cuts at: 3499 9266 3499
Size: 5767 4695
BstEII G'GTnAC_C
Cuts at: 1184 2843 6133 1184
Size: 1659 3290 5513
Fragments arranged by size:
5513 3290 1659
BstXI CCAn_nnnn'nTGG
Cuts at: 4114 5119 6371 4114
Size: 1005 1252 8205
Fragments arranged by size:
8205 1252 1005
BstYI r'GATC_y
Cuts at: 1972 2580 6939 7701 7792 8159 8544 8561 10013
Size: 608 4359 762 91 367 385 17 1452
Cuts at: 10013 10099 10111 10457 1972
Size: 86 12 346 1977
Fragments arranged by size:
4359 1977 1452 762 608 385 367 346
91 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3122 3644 6750 7574 3122
Size: 522 3106 824 6010
Fragments arranged by size:
6010 3106 824 522
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 2922 3162 3843 4471 4711
Size: 552 939 96 822 240 681 628 240
Cuts at: 4711 4866 5752 5852 6138 6219 513
Size: 155 886 100 286 81 4756
Fragments arranged by size:
4756 939 886 822 681 628 552 286
240 240 155 100 96 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6133 487
Size: 683 56 4907 4816
Fragments arranged by size:
4907 4816 683 56
BtsI GCAGTG_nn'
Cuts at: 2377 3191 3512 4748 4878 5765 6260 6376 7447
Size: 814 321 1236 130 887 495 116 1071
Cuts at: 7447 7978 8772 8792 2377
Size: 531 794 20 4047
Fragments arranged by size:
4047 1236 1071 887 814 794 531 495
321 130 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10462
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4159 4194 529
Size: 35 3595 35 6797
Fragments arranged by size:
6797 3595 35 35
DraI TTT'AAA
Cuts at: 7766 7766
Size: 10462
DraIII CAC_nnn'GTG
Cuts at: 3635 3635
Size: 10462
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9469 809
Size: 507 8153 1802
Fragments arranged by size:
8153 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2661 2919
Size: 54 1425 50 275 18 357 474 258
Cuts at: 2919 3087 3369 3411 3558 3825 4003 4414 4738
Size: 168 282 42 147 267 178 411 324
Cuts at: 4738 4745 5271 5339 5363 5424 6582 7390 7909
Size: 7 526 68 24 61 1158 808 519
Cuts at: 7909 8798 10427 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1158 889 808 526 519 474
411 357 324 282 275 267 258 178
168 147 68 61 54 50 43 42
24 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 5363 7909 1537
Size: 275 3551 2546 4090
Fragments arranged by size:
4090 3551 2546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2639 2963 8392 1353
Size: 1286 324 5429 3423
Fragments arranged by size:
5429 3423 1286 324
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2177 3566 4141 5831 6716 9049
Size: 1064 138 588 1389 575 1690 885 2333
Cuts at: 9049 9423 9569 387
Size: 374 146 1280
Fragments arranged by size:
2333 1690 1389 1280 1064 885 588 575
374 146 138
EcoICRI GAG'CTC
Cuts at: 2491 3748 4401 4683 5449 5571 2491
Size: 1257 653 282 766 122 7382
Fragments arranged by size:
7382 1257 766 653 282 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2597 2744 3038 3071 3527 3551
Size: 393 617 348 147 294 33 456 24
Cuts at: 3551 3761 4226 4403 4839 5108 5262 5361 5423
Size: 210 465 177 436 269 154 99 62
Cuts at: 5423 5435 5758 6209 6347 6448 6518 6554 6668
Size: 12 323 451 138 101 70 36 114
Cuts at: 6668 6728 6752 6973 7029 8525 9122 9909 10177
Size: 60 24 221 56 1496 597 787 268
Cuts at: 10177 1239
Size: 1524
Fragments arranged by size:
1524 1496 787 617 597 465 456 451
436 393 348 323 294 269 268 221
210 177 154 147 138 114 101 99
70 62 60 56 36 33 24 24
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 2548 2644 3545 4382 4805 5017 2054
Size: 494 96 901 837 423 212 7499
Fragments arranged by size:
7499 901 837 494 423 212 96
EcoO109I rG'GnC_Cy
Cuts at: 2212 2541 3178 3685 4263 4580 4628 4775 4839
Size: 329 637 507 578 317 48 147 64
Cuts at: 4839 4985 5027 5293 5718 6431 6614 6637 6772
Size: 146 42 266 425 713 183 23 135
Cuts at: 6772 6801 6802 6900 7241 2212
Size: 29 1 98 341 5433
Fragments arranged by size:
5433 713 637 578 507 425 341 329
317 266 183 147 146 135 98 64
48 42 29 23 1
EcoRI G'AATT_C
Cuts at: 6983 7771 8149 6983
Size: 788 378 9296
Fragments arranged by size:
9296 788 378
EcoRV GAT'ATC
Cuts at: 5849 5849
Size: 10462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 4283 4939 2200
Size: 2083 656 7723
Fragments arranged by size:
7723 2083 656
FspI TGC'GCA
Cuts at: 3692 5603 8968 3692
Size: 1911 3365 5186
Fragments arranged by size:
5186 3365 1911
HaeII r_GCGC'y
Cuts at: 1678 2020 2111 2157 2228 2396 2503 3434 4088
Size: 342 91 46 71 168 107 931 654
Cuts at: 4088 5154 5781 6219 7032 7539 9609 1678
Size: 1066 627 438 813 507 2070 2531
Fragments arranged by size:
2531 2070 1066 931 813 654 627 507
438 342 168 107 91 71 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2227 2259 4269 4301 4304
Size: 32 135 32 655 32 2010 32 3
Cuts at: 4304 4336 5240 5272 5285 5317 5377 5409 5924
Size: 32 904 32 13 32 60 32 515
Cuts at: 5924 5956 6380 6412 7437 7469 7551 7583 9103
Size: 32 424 32 1025 32 82 32 1520
Cuts at: 9103 9135 9177 9209 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2010 1520 1025 904 655 515 424
135 82 60 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 13 3
HincII GTy'rAC
Cuts at: 669 1657 1938 5995 7372 7806 8052 669
Size: 988 281 4057 1377 434 246 3079
Fragments arranged by size:
4057 3079 1377 988 434 281 246
HindIII A'AGCT_T
Cuts at: 748 5369 7628 748
Size: 4621 2259 3582
Fragments arranged by size:
4621 3582 2259
HpaI GTT'AAC
Cuts at: 8052 8052
Size: 10462
KasI G'GCGC_C
Cuts at: 2153 2392 2153
Size: 239 10223
KpnI G_GTAC'C
Cuts at: 6351 7787 6351
Size: 1436 9026
MfeI C'AATT_G
Cuts at: 8061 8061
Size: 10462
MluI A'CGCG_T
Cuts at: 6505 10338 6505
Size: 3833 6629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2583 2913 3237 3753 3817 4851 5784 9576
Size: 1278 330 324 516 64 1034 933 3792
Cuts at: 9576 9760 1305
Size: 184 2007
Fragments arranged by size:
3792 2007 1278 1034 933 516 330 324
184 64
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 2663 3560 4416 10
Size: 54 1425 343 357 474 897 856 6056
Fragments arranged by size:
6056 1425 897 856 474 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2921 3236 3977 4112 4118 4871 5117
Size: 648 1755 315 741 135 6 753 246
Cuts at: 5117 5564 5673 6552 7398 8420 8779 8938 518
Size: 447 109 879 846 1022 359 159 2042
Fragments arranged by size:
2042 1755 1022 879 846 753 741 648
447 359 315 246 159 135 109 6
MspA1I CmG'CkG
Cuts at: 1606 2102 2134 2461 2764 3025 3043 3097 3799
Size: 496 32 327 303 261 18 54 702
Cuts at: 3799 4162 4229 4833 5854 6082 6532 6919 7049
Size: 363 67 604 1021 228 450 387 130
Cuts at: 7049 7098 7192 7878 8555 9703 9948 1606
Size: 49 94 686 677 1148 245 2120
Fragments arranged by size:
2120 1148 1021 702 686 677 604 496
450 387 363 327 303 261 245 228
130 94 67 54 49 32 18
NaeI GCC'GGC
Cuts at: 2699 3569 4742 5271 5339 5489 2699
Size: 870 1173 529 68 150 7672
Fragments arranged by size:
7672 1173 870 529 150 68
NarI GG'CG_CC
Cuts at: 2154 2393 2154
Size: 239 10223
NcoI C'CATG_G
Cuts at: 513 1065 2004 2922 3843 4471 5752 513
Size: 552 939 918 921 628 1281 5223
Fragments arranged by size:
5223 1281 939 921 918 628 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10462
NgoMIV G'CCGG_C
Cuts at: 2697 3567 4740 5269 5337 5487 2697
Size: 870 1173 529 68 150 7672
Fragments arranged by size:
7672 1173 870 529 150 68
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10462
NotI GC'GGCC_GC
Cuts at: 7909 7909
Size: 10462
NspI r_CATG'y
Cuts at: 1132 1229 2743 7006 7498 7820 9365 1132
Size: 97 1514 4263 492 322 1545 2229
Fragments arranged by size:
4263 2229 1545 1514 492 322 97
PasI CC'CwG_GG
Cuts at: 1348 4138 4622 5659 6714 6886 1348
Size: 2790 484 1037 1055 172 4924
Fragments arranged by size:
4924 2790 1055 1037 484 172
PciI A'CATG_T
Cuts at: 9361 9361
Size: 10462
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3849 4622 4988 5311 7204 1108
Size: 2741 773 366 323 1893 4366
Fragments arranged by size:
4366 2741 1893 773 366 323
PfoI T'CCnGG_A
Cuts at: 2208 3543 3585 5400 5451 6464 6645 6902 6989
Size: 1335 42 1815 51 1013 181 257 87
Cuts at: 6989 7455 2208
Size: 466 5215
Fragments arranged by size:
5215 1815 1335 1013 466 257 181 87
51 42
PmeI GTTT'AAAC
Cuts at: 7766 7766
Size: 10462
PmlI CAC'GTG
Cuts at: 6055 6295 6055
Size: 240 10222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 8531 8563 10044 10076 3773
Size: 32 4726 32 1481 32 4159
Fragments arranged by size:
4726 4159 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 3178 4263 4628 4839 4985 5293 5718 6614
Size: 966 1085 365 211 146 308 425 896
Cuts at: 6614 6637 6900 2212
Size: 23 263 5774
Fragments arranged by size:
5774 1085 966 896 425 365 308 263
211 146 23
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10462
PsiI TTA'TAA
Cuts at: 4896 8032 4896
Size: 3136 7326
PspOMI G'GGCC_C
Cuts at: 4774 6801 4774
Size: 2027 8435
PspXI vC'TCGA_Gb
Cuts at: 2093 6167 2093
Size: 4074 6388
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5576 5608 5576
Size: 32 10430
PstI C_TGCA'G
Cuts at: 830 2613 2622 2790 3102 3186 3207 3354 5627
Size: 1783 9 168 312 84 21 147 2273
Cuts at: 5627 5726 7301 7814 830
Size: 99 1575 513 3478
Fragments arranged by size:
3478 2273 1783 1575 513 312 168 147
99 84 21 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10462
PvuII CAG'CTG
Cuts at: 2134 2461 2764 3025 3097 3799 6532 6919 2134
Size: 327 303 261 72 702 2733 387 5677
Fragments arranged by size:
5677 2733 702 387 327 303 261 72
SacI G_AGCT'C
Cuts at: 2493 3750 4403 4685 5451 5573 2493
Size: 1257 653 282 766 122 7382
Fragments arranged by size:
7382 1257 766 653 282 122
SacII CC_GC'GG
Cuts at: 2103 5855 2103
Size: 3752 6710
SalI G'TCGA_C
Cuts at: 1936 7370 7804 1936
Size: 5434 434 4594
Fragments arranged by size:
5434 4594 434
SanDI GG'GwC_CC
Cuts at: 4263 4628 4263
Size: 365 10097
SbfI CC_TGCA'GG
Cuts at: 7814 7814
Size: 10462
ScaI AGT'ACT
Cuts at: 8710 10422 8710
Size: 1712 8750
SexAI A'CCwGG_T
Cuts at: 3324 3324
Size: 10462
SfcI C'TryA_G
Cuts at: 826 1045 2317 2609 2618 2786 3098 3182 3203
Size: 219 1272 292 9 168 312 84 21
Cuts at: 3203 3350 5623 5722 7297 7810 8945 9626 9817
Size: 147 2273 99 1575 513 1135 681 191
Cuts at: 9817 826
Size: 1471
Fragments arranged by size:
2273 1575 1471 1272 1135 681 513 312
292 219 191 168 147 99 84 21
9
SfoI GGC'GCC
Cuts at: 2155 2394 2155
Size: 239 10223
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10462
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10462
SmaI CCC'GGG
Cuts at: 1872 2477 3951 5176 7789 1872
Size: 605 1474 1225 2613 4545
Fragments arranged by size:
4545 2613 1474 1225 605
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2093 2174 3383 3749 4009 6167
Size: 197 926 150 81 1209 366 260 2158
Cuts at: 6167 7310 7631 8566 9467 9729 820
Size: 1143 321 935 901 262 1553
Fragments arranged by size:
2158 1553 1209 1143 935 926 901 366
321 262 260 197 150 81
SnaBI TAC'GTA
Cuts at: 493 8253 493
Size: 7760 2702
SpeI A'CTAG_T
Cuts at: 152 5138 152
Size: 4986 5476
SphI G_CATG'C
Cuts at: 1132 7820 1132
Size: 6688 3774
SspI AAT'ATT
Cuts at: 5 52 8386 5
Size: 47 8334 2081
Fragments arranged by size:
8334 2081 47
StuI AGG'CCT
Cuts at: 1517 4652 4796 6031 7225 7325 1517
Size: 3135 144 1235 1194 100 4654
Fragments arranged by size:
4654 3135 1235 1194 144 100
StyI C'CwwG_G
Cuts at: 513 1065 2004 2157 2190 2664 2922 3843 4471
Size: 552 939 153 33 474 258 921 628
Cuts at: 4471 4848 5752 6129 6609 6768 7104 7128 7901
Size: 377 904 377 480 159 336 24 773
Cuts at: 7901 513
Size: 3074
Fragments arranged by size:
3074 939 921 904 773 628 552 480
474 377 377 336 258 159 153 33
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3233 3371 4130 4781 5765 8489 8506
Size: 1255 1653 138 759 651 984 2724 17
Cuts at: 8506 8659 8844 9289 325
Size: 153 185 445 1498
Fragments arranged by size:
2724 1653 1498 1255 984 759 651 445
185 153 138 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3288 3297 6282
Size: 274 80 33 51 1443 1311 9 2985
Cuts at: 6282 7641 8708 10420 96
Size: 1359 1067 1712 138
Fragments arranged by size:
2985 1712 1443 1359 1311 1067 274 138
80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2394 2426 3170 3202 3619 3651 4508 4540 5399
Size: 32 744 32 417 32 857 32 859
Cuts at: 5399 5431 6305 6337 10422 10454 2394
Size: 32 874 32 4085 32 2402
Fragments arranged by size:
4085 2402 874 859 857 744 417 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3326 4372 7042 1316
Size: 2010 1046 2670 4736
Fragments arranged by size:
4736 2670 2010 1046
XbaI T'CTAG_A
Cuts at: 7798 7798
Size: 10462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2929 3421 5782 6136 6271 6331 6536 2068
Size: 861 492 2361 354 135 60 205 5994
Fragments arranged by size:
5994 2361 861 492 354 205 135 60
XhoI C'TCGA_G
Cuts at: 1943 2093 6167 1943
Size: 150 4074 6238
Fragments arranged by size:
6238 4074 150
XmaI C'CCGG_G
Cuts at: 1870 2475 3949 5174 7787 1870
Size: 605 1474 1225 2613 4545
Fragments arranged by size:
4545 2613 1474 1225 605
XmnI GAAnn'nnTTC
Cuts at: 3495 4388 8591 3495
Size: 893 4203 5366
Fragments arranged by size:
5366 4203 893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8270 276
Size: 53 83 186 936 6736 2468
Fragments arranged by size:
6736 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI
NruI NsiI PacI RsrII SapI SfiI SrfI SwaI